Summary
Brush-border membrane vesicles (BBMV) from rat kidney cortex possessed two uptake systems for γ-aminobutyric acid (GABA), a high affinity system (Km=10.9 μM) and a low affinity system (Km=1203 μM). Both uptake systems were inhibited by p-hydroxymercuribenzoic acid and ouabain, and by the action of neuraminidase, whereas the GABA analogs nipecotic acid, β-alanine, 2,4-diaminobutyric acid and 4,5,6,7-tetrahydroisoxazolo-[4,5c]-pyridin-3-ol had no effect on the GABA uptake activity. The BBMW uptake systems were clearly different from the GABA transport systems present in brain tissue.
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References
Levi, G., and Raiteri, Life Sci.12 (1973) 81.
Wood, J. D., and Sidhu, H. S., J. Neurochem.46 (1986) 739.
Wood, J. D., and Sidhu, H. S., J. Neurochem.49 (1987) 1202.
Goodyer, P. R., Rozen, R., and Scriver, C. R., Biochim. biophys. Acta818 (1985) 45.
Zaleska, M. M., and Erecinska, M., Proc. natl Acad. Sci.84 (1987) 1709.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. biol. Chem.193 (1951) 265.
Hartree, E. F., Analyt. Biochem.48 (1972) 422.
Munson, P. J., and Rodbard, D., Analyt. Biochem.107 (1980) 220.
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Sidhu, H.S., Wood, J.D. Gamma-aminobutyric acid uptake by rat kidney brush-border membrane vesicles. Experientia 45, 726–728 (1989). https://doi.org/10.1007/BF01974569
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DOI: https://doi.org/10.1007/BF01974569