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Endocytosis and inositol hexakisphosphate levels inras transformants ofDictyostelium discoideum amoebae

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Summary

Fluid-phase pinocytosis kinetics and lysosomal enzyme secretion parameters were measured inDictyostelium discoideum amoebae constructed from strain AX3 by transformation with a multicopy plasmid carrying either a normalras gene (ras-Gly12), a mutatedras gene (ras-Thr12) or by the vector carrying the geneticin resistance gene only (pDNEO2). It was found that the pinocytosis rate and extent as well as the lysosomal enzyme secretion were slightly different in the three strains. These changes, however, were related to minor modifications of the cellular volumes. The overall concentration of inositol hexakisphosphate was similar in the three strains.

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Authors and Affiliations

  1. DRF/LBIO/Biochimie (UA 1130 CNRS), 85 X, F-38041, Grenoble cedex, France

    G. Klein & M. Satre

  2. DRF/SPh/Résonance Magnétique en Biologie et Médecine, Centre d'Etudes Nucléaires, 85X, F-38041, Grenoble cedex, France

    J. -B. Martin

  3. Swiss Institute for Experimental Cancer Research (ISREC), CH-1066, Epalinges, Switzerland

    C. Reymond

Authors
  1. G. Klein
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  2. J. -B. Martin
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  3. M. Satre
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  4. C. Reymond
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Additional information

This work was supported in part by a grant from the Ligue Nationale Française contre le Cancer (no 880258) to MS, and by a grant from the Fonds National Suisse de la Recherche Scientifique (No. 3.623-087) to CR.

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Klein, G., Martin, J.B., Satre, M. et al. Endocytosis and inositol hexakisphosphate levels inras transformants ofDictyostelium discoideum amoebae. Experientia 45, 365–367 (1989). https://doi.org/10.1007/BF01957480

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  • DOI: https://doi.org/10.1007/BF01957480

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