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A comparison of cycloartenol and lanosterol biosynthesis and metabolism by the Oomycetes

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Summary

Representative members in each of the four orders of Oomycetes (Phytophthora cactorum, Peronosporales;Lagenidium callinectes, L. giganteum, Lagenidiales;Saprolegnia ferax, Saprolegniales;Apodachylella completa, Leptomitales) have been examined for their ability to synthesize and polycyclize squalene-oxide (SO) to a tetracyclic product and to differentiate between cycloartenol and lanosterol metabolism to sterols.P. cactorum andL. giganteum failed to synthesize or metabolize SO, cycloartenol or lanosterol. While the other three fungi synthesized sterols via SO and lanosterol, a minor metabolism of added cycloartenol to the 4,4-desmethyl-14α-methylcyclosteroid dehydropollinastanol was observed.

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Authors and Affiliations

  1. Plant Physiology and Chemistry Research Unit, ARS-US Department of Agriculture, 94710, Berkeley, California, USA

    W. D. Nes, P. H. Le, L. R. Berg, G. W. Patterson & J. L. Kerwin

  2. Department of Botany, University of Maryland, 20742, College Park, Maryland, USA

    W. D. Nes, P. H. Le, L. R. Berg, G. W. Patterson & J. L. Kerwin

  3. Department of Entomology, University of California, 95616, Davis, California, USA

    W. D. Nes, P. H. Le, L. R. Berg, G. W. Patterson & J. L. Kerwin

Authors
  1. W. D. Nes
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  2. P. H. Le
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  3. L. R. Berg
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  4. G. W. Patterson
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  5. J. L. Kerwin
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Additional information

Acknowledgment.Saprolegnia ferax-ATCC 3605 (1),Lagenidium callinectes ATCC 24973 (2),Apodachlyella completa (3) were obtained from Dr J. Aronson, Arizona State Univ. (3),Lagenidium gigateum was obtained from a drainage ditch in California (4), andPhytophthora cactorum was obtained from the U.C. Berkeley Fungal Collection (5). The fungi were cultured as previously described: Cultures 1,2,3: Berg, L. B., Ph. D. dissertation, Univ. of MD., College Park (1983); culture 4: Kerwin, J.L., and Washino, R.K., Exp. Mycol.7 (1983) 109; culture 5: Nes, W.D., and Stafford, A.E., Lipids19 (1984) 544. Preliminary observations involving the labeled substrates were presented by Le, P.H., Nes, W.D., and Parish, E.J. J. Am. Oil Chem. Soc.62 (1985) 655(A). Please address all correspondence to W.D. Nes, Plant Physiology and Chemistry Research Unit, ARS-US Dept of Agriculture, Berkeley, CA 94710, USA.

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Nes, W.D., Le, P.H., Berg, L.R. et al. A comparison of cycloartenol and lanosterol biosynthesis and metabolism by the Oomycetes. Experientia 42, 556–558 (1986). https://doi.org/10.1007/BF01946701

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  • DOI: https://doi.org/10.1007/BF01946701

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