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Differential sensitivity to ethidium bromide of replicative DNA synthesis and bleomycin-induced unscheduled DNA synthesis in permeable mouse sarcoma cells1

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Summary

Replicative DNA synthesis in permeable mouse sarcoma cells was more sensitive to ethidium bromide (EtBr) than bleomycin-induced unscheduled DNA synthesis (UDS). A similar difference in sensitivity to EtBr was observed between DNA polymerases α and β. The difference in sensitivity to EtBr of replicative DNA synthesis and UDS in the present system seems to reflect mainly the sensitivity difference between DNA polymerases α and β.

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Literatur

  1. Meyer, R. R., and Simpson, M. V., Biochem. biophys. Res. Commun.34 (1969) 238.

    Article  CAS  PubMed  Google Scholar 

  2. Friedlender, B., and Weissbach, A., Proc. natl Acad. Sci. USA68 (1971) 3116.

    Article  Google Scholar 

  3. Hirschman, S. Z., Science173 (1971) 441.

    Article  CAS  PubMed  Google Scholar 

  4. Bolden, A., Noy, G. P., and Weissbach, A., J. biol. Chem.252, (1977) 3351.

    Article  CAS  PubMed  Google Scholar 

  5. Cozzarelli, N. R., A. Rev. Biochem.46 (1977) 641.

    Article  CAS  Google Scholar 

  6. Fujisawa, T., Tanaka, S., Kobayashi, M., and Koike, K., Biochim. biophys. Acta475 (1977) 611.

    Article  CAS  PubMed  Google Scholar 

  7. Hirschman, S. Z., and Garfinkel, E., Nature271 (1978) 681.

    Article  CAS  PubMed  Google Scholar 

  8. Tarrago-Litvak, L., Viratelle, O., Darriet, D., Dalibart, R., Graves, P. V., and Litvak, S., Nucl. Acids Res.5 (1978) 2197.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Mattern, M. R., and Painter, R. B., Biochim. biophys. Acta563 (1979) 293.

    Article  CAS  PubMed  Google Scholar 

  10. Sarih, L., Hevia-Campos, E., Tharaud, D., and Litvak, S., FEBS Lett.122 (1980) 100.

    Article  CAS  PubMed  Google Scholar 

  11. Kaufman, D. G., Grisham, J. W., and Stenstrom, M. L., Biochim. biophys. Acta272 (1972) 212.

    Article  CAS  Google Scholar 

  12. Seki, S., Oda, T., and Ohashi, M., Biochim. biophys. Acta610 (1980) 413.

    Article  CAS  PubMed  Google Scholar 

  13. Seki, S., and Mueller, G. C., Biochim. biophys. Acta435 (1976) 236.

    Article  CAS  PubMed  Google Scholar 

  14. Chang, L. M. S., J. biol. Chem.248 (1973) 3789.

    Article  CAS  PubMed  Google Scholar 

  15. Seki, S., and Oda, T., Biochim. biophys. Acta479 (1977) 391.

    Article  CAS  PubMed  Google Scholar 

  16. Seki, S., and Oda, T., Biochim. biophys. Acta521 (1978) 520.

    Article  CAS  PubMed  Google Scholar 

  17. Huberman, J. A., Cell23 (1981) 647.

    Article  CAS  PubMed  Google Scholar 

  18. Waring, M. J., J. molec. Biol.13 (1965) 269.

    Article  CAS  PubMed  Google Scholar 

  19. Lurquin, P. F., and Seligy, V. L., Biochem. biophys. Res. Commun.46 (1972) 1399.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Cancer Institute, Okayama University Medical School, 2-5-1, Shikato-cho, 700, Okayama, Japan

    S. Seki & T. Oda

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  1. S. Seki
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  2. T. Oda
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Additional information

Acknowledgments. The authors wish to thank Nippon Kayaku Co. (Tokyo, Japan) for providing copper-free bleomycin A2. This research was supported in part by a grant from the Japan Ministry of Education, Science and Culture.

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Seki, S., Oda, T. Differential sensitivity to ethidium bromide of replicative DNA synthesis and bleomycin-induced unscheduled DNA synthesis in permeable mouse sarcoma cells1. Experientia 40, 869–871 (1984). https://doi.org/10.1007/BF01952001

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