Summary
The rate of development of Ruhemann's purple in the ninhydrin reaction of two deuterated primary amines, αα-d2-p-tyramine and αα-d2-β-phenylethylamine, is significantly reduced It appears to be a primary isotope effect and indicates that the cleavage of the carbon-hydrogen bond at the α-position is involved in the rate-determining step of the color reaction.
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References
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Acknowledgements. We thank Dr A.A. Boulton for his advice and encouragement and the Canadian Medical Research Councel and Saskatchewan Health for their continuing financial support.
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Yu, P.H., Davis, B.A. Deuterium isotope effects in the ninhydrin reaction of primary amines. Experientia 38, 299–300 (1982). https://doi.org/10.1007/BF01949352
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DOI: https://doi.org/10.1007/BF01949352