Summary
A method for a screening program for haemoglobinopathies in a starch agar gel mixed with saponin is presented. Normal and abnormal blood containing haemoglobins S, C, I, M Boston, D Punjab, beta thalassaemia major and beta thalassaemia minor, were applied, in a tray with the capacity for 100 samples. The electrophoresis was performed in 45 min using 300 V. This method offers special advantages for the examination of a large number of samples, using a small amount of whole blood and without the previous preparation of haemoglobin solution.
Similar content being viewed by others
References
G. Stamatoyannopoulos, A. Rev. Genet.6, 47 (1972).
R. M. Schmidt, J. Am. med. Ass.224, 1276 (1973).
M. D. Garrick, P. Dembure and R. Guthrie, N. Engl. J. Med.288, 1265 (1973).
R. M. Schmidt and S. Holland, Clin. Chem.20, 591 (1974).
H. Lehmann and R. G. Hunstman, in: Man's Haemoglobins, p. 478.
P. F. Milner and H. Gooden, Am. J. clin. Path.64, 58 (1975).
C. Baglioní, Biochim. biophys. Acta48, 253 (1962).
J. E. Cradock-Watson, J. C. B. Fenton and H. Lehmann, J. clin. Path.12, 372 (1959).
M. D. Poulik, Nature180, 1477 (1957).
Author information
Authors and Affiliations
Additional information
Acknowledgments. We would like to express our gratitude to Dr C. Daghlian and Dr Lewis J. Greene for their valuable suggestions. We also thank the Conselho Nacional do Desenvolvimento Científico e Tecnológico, CNPq, for financial support.
Rights and permissions
About this article
Cite this article
Naoum, P.C., Campos, M.C.R.M., Parenti, M.F. et al. An improved electrophoretic method for a screening program for haemoglobinopathies. Experientia 36, 875–876 (1980). https://doi.org/10.1007/BF01978625
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01978625