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The inability of nuclear dehydrogenating clostridia to oxidize bile salt hydroxyl groups

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Summary

In a survey of the intracellular bile salt oxidoreductase activity in fecal bacteria, 16 strains of nuclear dehydrogenating clostridia and 2 strains of non-nuclear dehydrogenatingC. paraputrificum were demonstrated unable to oxidize cholate at any of the 3 OH groups. Since nuclear dehydrogenation at the Δ-1 and Δ-4 position requires a 3-oxo precursor steroid, it appears that these organisms require the presence of a 3α-hydroxysteroid dehydrogenating organism for nuclear dehydrogenation.

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Authors and Affiliations

  1. Department of Medicine, Dalhousie University Halifax, B3H 1W2, Nova Scotia, Canada

    I. A. Macdonald & M. J. Hill

  2. Bacterial Metabolism Research Laboratory Colindale Hospital, NW9 5DX, London, England

    I. A. Macdonald & M. J. Hill

Authors
  1. I. A. Macdonald
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  2. M. J. Hill
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Additional information

This work is supported by the National Cancer Institute of Canada (IAM) and by the Cancer Research Campaign (MJH).

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Macdonald, I.A., Hill, M.J. The inability of nuclear dehydrogenating clostridia to oxidize bile salt hydroxyl groups. Experientia 35, 722–723 (1979). https://doi.org/10.1007/BF01968203

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