Skip to main content
SpringerLink
Log in

Affinity chromatography of soluble galactosyltransferases using an easily synthesized N-acetylglucosamine-agarose bead

  • Specialia
  • Published:
Experientia Aims and scope Submit manuscript

Summary

Agarose derivatized withp-nitrophenyl-N-acetyl-β-d-glucosamine was used for affinity chromatography for soluble galactosyltransferase from various sources. Moreover, this adsorbant acts as an acceptor for galactose when incubated with prepurified galactosyltransferase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. R. Barker, K. W. Olsen, J. H. Shaper andR. L. Hill, J. biol. Chem.247, 7135 (1972).

    Article  CAS  PubMed  Google Scholar 

  2. R. Bloch andM. M. Burger, FEBS Lett.44, 286 (1974).

    Article  CAS  PubMed  Google Scholar 

  3. Y. S. Kim, J. Perdomo, J. S. Whitehead andK. J. Curtis, J. clin. Invest.51, 2033 (1972).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. D. K. Podolsky andM. M. Weiser, Biochem. J.146, 213 (1975).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. W. E. Treveylan, D. P. Procter andJ. S. Harrison, J. biol. Chem.245, 4150 (1970).

    Article  Google Scholar 

  6. O. H. Lowry, N. J. Rosebrough, A. L. Farr andR. J. Randall, J. biol. Chem.193, 265 (1951).

    Article  CAS  PubMed  Google Scholar 

  7. I. P. Trayer andR. L. Hill, J. biol. Chem.246, 6666 (1971).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medicine, Harvard Medical School and Gastrointestinal Unit, Massachusetts General Hospital, 02114, Boston, Massachusetts, USA

    E. G. Berger (EGB is a Fellow sponsored by the Schweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung), M. M. Weiser & K. J. Isselbacher

Authors
  1. E. G. Berger
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. M. Weiser
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. J. Isselbacher
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by a grant from the USPHS (grant No. 1 PO1 CA 14294-2).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Berger, E.G., Weiser, M.M. & Isselbacher, K.J. Affinity chromatography of soluble galactosyltransferases using an easily synthesized N-acetylglucosamine-agarose bead. Experientia 32, 690–691 (1976). https://doi.org/10.1007/BF01919834

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01919834

Keywords

Search

Navigation