Abstract
Newly isolated strains Bacillus sp. FME 1 and FME 2 were evaluated for the cellulolytic enzymes production during submerged fermentation (SmF) of different substrates including rice husk, Whatman filter paper and cellulose powder CF 11. Extracellular enzyme assays for CMCase, FPase and β-glucosidase were examined up to 8 days of submerged fermentation. Among the three substrates, rice husk was the most suitable substrate for higher production of cellulolytic enzymes. Maximum titers of 100, 45, and 3.5 U/mL in respect of CMCase, FPase and β-glucosidase in Bacillus sp. FME 2 were recovered as against 45, 12, and 0.39 U/mL in Bacillus sp. FME 1 respectively, at their respective peak time intervals. Bacillus sp. FME 2 was found to produce higher cellulolytic enzyme activities than Bacillus sp. FME 1.
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Abbreviations
- CMC:
-
carboxymethyl cellulose
- CMCase:
-
carboxymethyl cellulase
- DNS:
-
dinitrosalicylic acid
- FME:
-
flour mill effluent
- FPase:
-
filter paperase
- PNPG:
-
p-nitrophenyl-β-D-glucopyranoside
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The first two authors contributed equally to this work and should be considered as joint first authors.
The first two authors contributed equally to this work and should be considered as joint first authors.
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Kumar, G.S., Chandra, M.S., Sumanth, M. et al. Cellulolytic Enzymes Production from Submerged Fermentation of Different Substrates by Newly Isolated Bacillus spp. FME. J. Korean Soc. Appl. Biol. Chem. 52, 17–21 (2009). https://doi.org/10.3839/jksabc.2009.003
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DOI: https://doi.org/10.3839/jksabc.2009.003