Stable hydrogen isotopes in monsoonal precipitation (δDp) at three sites (Port Blair, Barisal and Darjeeling) reveal the factors governing δDp variations over a south-north gradient across the Bay of Bengal. We found that the δDp at each site continuously decreases from May to September and these trends become more pronounced from south to north. The decreasing trends of downstream δDp closely follow the decreasing trends of upstream stable hydrogen isotopes in water vapor (δDv), which indicates that upstream δDv properties shape initial spatiotemporal patterns of the downstream δDp (“shaping effect”). Additionally, our results demonstrate that, during moisture transport, upstream vertical air motions (convection and downward motion) and topographic relief magnify the amplitude of the decreasing trends of downstream δD (“magnifying effect”). Our findings imply that upstream δD properties and relevant atmospheric and pv topographical conditions along the moisture transport pathway need to be considered collectively to better interpret paleoclimate records.