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Bacillus stearothermophilus spores on strips. Calibration of thermoresistance kinetics to prior biological validation

  • Biotechnology
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Abstract

Filter-paper strips saturated with Bacillus stearothermophilus spores were prepared and then stored for up to 1470 days. The strips were used to measures spore activation time and death kinetics in two systems: a serum bottle/oil bath combination, and glassine packages in a gravity autoclave. The data collected were analyzed primarily by analysis of variance and the interaction of spore storage time, processing temperature, inertial time and exposure time was determined.

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Authors and Affiliations

  1. Departamento de Technologia Bioquímico-Farmacêutica, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, P.O. Box 66083, 05389-970, São Paulo, Brasil

    T. C. Vessoni Penna, I. A. Machoshvili & E. Aquarone

Authors
  1. T. C. Vessoni Penna
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  2. I. A. Machoshvili
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  3. E. Aquarone
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Vessoni Penna, T.C., Machoshvili, I.A. & Aquarone, E. Bacillus stearothermophilus spores on strips. Calibration of thermoresistance kinetics to prior biological validation. Appl Microbiol Biotechnol 46, 340–346 (1996). https://doi.org/10.1007/BF00166227

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  • DOI: https://doi.org/10.1007/BF00166227

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