Summary
The influence of inorganic phosphate and immobilization on cells of Claviceps purpurea strain 1029/N5 producing ergot peptides in shake culture was examined. Immobilization in Ca-alginate beads resulted in a marked reduction of some metabolic activities, i.e. the periods of alkaloid formation and cell growth were prolonged. High concentrations of inorganic phosphate (1 g/l KH2PO4) could reduce or stop alkaloid formation both by free and immobilized cells at any time during fermentation. The optimum phosphate concentration for alkaloid production by immobilized cells (about 0.5 mM) was a quarter of that required by free cells. This optimum shift was attributed to (i) the diminished phosphate demand of immobilized cells, due to their reduced metabolic activities, and (ii) the phosphate-dependent morphological behaviour of the biocatalyst. The observed decrease in alkaloid concentrations during later periods of the fermentation supported the idea of alkaloid-degradative enzymes, activated by high phosphate concentrations. Immobilization showed an advantageous influence on this undesirable effect.
Similar content being viewed by others
References
Amici AM, Minghetti A, Scotti T, Spalla C, Tognoli L (1966) Production of ergotamine by a strain of Claviceps purpurea (Fr.) Tul. Experientia 22:415–416
Amici AM, Minghetti A, Scotti T, Spalla C, Tognoli L (1967) Ergotamine production in submerged culture and physiology of Claviceps purpurea. Appl Microbiol 15:597–602
Bethke H, Delz B, Stich K (1976) Determination of the content and purity of ergotamine preparations by means of high-pressure-liquid chromatography. J Chromatogr 123:193–203
Beunink J, Rehm H-J (1988) Synchronous anaerobic and aerobic degradation of DDT by an immobilized mixed culture system. Appl Microbiol Biotechnol 29:72–80
Beunink J, Baumgärtl H, Zimelka W, Rehm H-J (1989) Determination of oxygen gradients in single Ca-alginate beads by means of oxygen-microelectrodes. Experientia (in press)
Demain AL (1972) Cellular and environmental factors affecting the synthesis and excretion of metabolites. J Appl Chem Biotechnol 22:345–362
Eikmeier H, Rehm H-J (1987) Stability of calcium-alginate during citric acid production of immobilized Aspergillus niger. Appl Microbiol Biotechnol 26:105–111
Eikmeier H, Westmeier F, Rehm H-J (1984) Morphological development of Aspergillus niger immobilized in Ca-alginate and k-carrageenan. Appl Microbiol Biotechnol 19:53–57
Fiske CH, Subbarow Y (1925) The colorimetric determination of phosphorus. J Biol Chem 66:375–400
Gosmann B, Rehm H-J (1986) Oxygen uptake of microorganisms entrapped in Ca-alginate. Appl Microbiol Biotechnol 23:163–167
Gosmann B, Rehm H-J (1988) Influence of growth behaviour and physiology of alginate-entrapped microorganisms on the oxygen consumption. Appl Microbiol Biotechnol 29:554–559
Hartmeier W, Schmitz M (1987) Peptide alkaloids using immobilized mycelia of Claviceps purpurea. In: Chmiel H, Hammes WP, Bailey JE (eds) Biochemical engineering. Fischer, Stuttgart, pp 469–473
Keller U (1983) Highly efficient mutagenesis of Claviceps purpurea by using protoplasts. Appl Environ Microbiol 46:580–584
Keweloh H, Heipieper HJ, Rehm H-J (1989) Protection of bacteria against toxicity of phenol by immobilization in calcium alginate. Appl Microbiol Biotechnol 31:383–389
Kobel H, Sanglier JJ (1986) Ergot alkaloids. In: Pape H, Rehm H-J (eds) Biotechnology, vol 4, VCH, Weinheim, pp 569–609
Kopp B (1987) Long-term alkaloid production by immobilized cells of Claviceps purpurea. Methods Enzymol 136:317–329
Kopp B, Rehm H-J (1983) Alkaloid production by immobilized mycelia of Claviceps purpurea. Eur J Appl Microbiol Biotechnol 18:257–263
Kopp B, Rehm H-J (1984) Semicontinuous cultivation of immobilized Claviceps purpurea. Appl Microbiol Biotechnol 19:141–145
Kusch J, Rehm H-J (1986) Regulation aspects of roquefortine production by free and Ca-alginate immobilized mycelia of Penicillium roqueforti. Appl Microbiol Biotechnol 23:394–399
Martin JF (1977) Control of antibiotic synthesis by phosphate. In: Ghose TK, Fiechter A, Blakebrough N (eds) Advances in biochemical engineering, vol 6, Springer, Berlin, Heidelberg, New York, pp 105–127
Michelon LE, Kelleher WJ (1963) Production of lysergic acid derivatives in submerged cultures. A modified procedure using paradimethylaminobenzaldehyde. Lloydia 26:192–201
Pazoutova S, Votruba J, Rehacek Z (1981) A mathematical model of growth and alkaloid production in the submerged culture of Claviceps purpurea. Biotechnol Bioeng 23:2837–2849
Rehacek Z, Sajdl P, Kozova J, Malik KA, Ricicova A (1971) Correlation of certain alterations in metabolic activity with alkaloid production by submerged Claviceps. Appl Microbiol 22:946–956
Robbers JE, Robertson LW, Hornemann KM (1972) Physiological studies on ergot: further studies on the induction of alkaloid synthesis by trytophan and its inhibition by phosphate. J Bacteriol 112:791–796
Robbers JE, Eggert WW, Floss HG (1978) Physiological studies on ergot: time factor influence on the inhibitory effect of phosphate and the induction effect of tryptophan on alkaloid production. Lloydia 41:120–129
Taber WA (1985) Biology of Claviceps. Biotechnology Series 6 (Biology of industrial organisms):449-486
Author information
Authors and Affiliations
Additional information
Offprint requests to: H.J. Rehm
Rights and permissions
About this article
Cite this article
Lohmeyer, M., Dierkes, W. & Rehm, HJ. Influence of inorganic phosphate and immobilization on Claviceps purpurea . Appl Microbiol Biotechnol 33, 196–201 (1990). https://doi.org/10.1007/BF00176524
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00176524