Abstract
Spores of Streptomyces melanosporofaciens EF-76, an actinomycete that inhibits the growth of several plant pathogens, were incorporated in beads of chitosan and polyphosphate using the entrapment technique called complex coacervation. The degradation of spore-loaded beads was monitored by measuring the residual amount of chitosan in soil and by enumerating the S. melanosporofaciens population over time. After the introduction of spore-loaded chitosan beads into soil, the amount of chitosan in sterile soil remained at 1.550 mg/g throughout the first week and diminished to 0.101 mg/g after 7 weeks. Bead degradation proceeded faster in non-sterile soil but a progressive release of both chitosan oligomers and the antagonistic microbial agent was nevertheless observed. Application of these spore-loaded chitosan beads to seed potato tubers protected progeny tubers against common scab.
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Acknowledgements
This work was supported by a grant from the Conseil de Recherches en Pêches et en Agroalimentaire du Québec and the Natural Sciences and Engineering Research Council of Canada. Chitosan was kindly donated by Marinard Biotech. We thank J. Beauséjour and N. Clermont for their technical assistance and B. Talbot for a critical review of the manuscript
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Jobin, G., Couture, G., Goyer, C. et al. Streptomycete spores entrapped in chitosan beads as a novel biocontrol tool against common scab of potato. Appl Microbiol Biotechnol 68, 104–110 (2005). https://doi.org/10.1007/s00253-004-1843-8
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DOI: https://doi.org/10.1007/s00253-004-1843-8