In recent years, the efficient removal of organic pollutants from wastewater has emerged as a critical area of global research interest. Against this backdrop, an array of innovative technologies for wastewater treatment has been developed. Among numerous advanced oxidation processes (AOPs), periodate (PI), an emerging oxidizing agent in AOPs, has garnered significant attention from researchers. Particularly, the integration of ultrasound (US)-activated PI systems has been recognized as an exceptionally promising approach for the synergistic degradation of organic pollutants in wastewater. In this paper, we conducted a thorough analysis of the mechanisms underlying the degradation of organic pollutants using the US/PI system. Furthermore, we comprehensively delineated the effects of ultrasonic power, periodate concentration, temperature, pH, coexisting inorganic ions, and dissolved organic matter on the removal efficiency of organic pollutants and summarized application cases of the US/PI system for the degradation of different pollutants. Finally, we also offered prospective discussions on the future trajectories of US/PI technology development. Full article

International conservation approaches recognise architectural heritage as crucial in promoting sustainable development and enhancing human well-being. This has been highlighted by the recent New European Bauhaus (NEB) movement. As for the reuse of architectural heritage, this debate has led to the formulation of new guidelines that aim to maximise the tangible and intangible values of the assets from a long-term and circular economy perspective. In turning theory into operational practice, it is essential to reuse heritage structures while keeping these principles in mind and remaining within the boundaries of conservation objectives. To achieve this, evaluation tools that can aid in the decision-making process need to be identified. This research presents a novel model of indicators that can monitor and evaluate the quality of architectural heritage reuse projects, proposing a perspective that considers both the concept of well-being and the NEB principles. The proposed model is tested on Turin’s IPIM (Provincial Institute for Childhood and Maternity). This structure has been transformed from an uncomfortable heritage into a cultural centre for contemporary art called Flashback Habitat. The case study tests the model’s practical applicability and demonstrates its effectiveness in identifying the most challenging principles to apply in practice. Full article

In West Africa, the construction of small earth dams is common against water scarcity. Burkina Faso, an inland country in West Africa, is home to 1001 dams that serve agricultural and pastoral needs. These embankments are predominantly made of compacted laterite, a cost-effective material abundant in over 2/3 of the country. However, these dams degrade over time, hindering their functionality. This study aims to establish a catalog of typical degradation occurring on small dams in Burkina Faso, which is virtually non-existent in the region while identifying and analyzing the potential causes. The study uses a diagnostic analysis followed up with technical visits on a representative sample of 24 dams in the Centre and Centre-South regions as a basis for future studies. The results reveal that these dams were constructed between 1965 and 2018, with capacities ranging from 150,000 to 4,740,000 m³. 33% of these dams have undergone total failure, likely attributed to factors such as internal erosion, pore overpressures, settlement, and deformation. Although 67% of the dams remain functional, their structural integrity could be improved. Erosion observed in riprap indicates vulnerability during high flood periods. Additionally, the absence of proper maintenance, as shown by the vegetation development weakening embankments, contributes to deterioration. The analysis also suggests that variability in construction techniques and lateritic material properties across time and regions may further exacerbate degradation. These findings inform infrastructure improvements and policy development for sustainable water resource management in Burkina Faso and similar regions. Full article

Hybrid lateral closed-wedge high tibial osteotomy (HBHTO) carries certain advantages over medial open-wedge high tibial osteotomy (OWHTO). We investigated the potential difference in the required correction angle between HBHTO and OWHTO to achieve an equal amount of whole lower-extremity alignment correction, retrospectively analyzing the preoperative plain radiographic images of 100 patients. The medial proximal tibial angle (MPTA), joint line convergence angle (JLCA), mechanical lateral distal femoral angle (mLDFA), hip–knee–ankle axis (HKA), length of the tibia, width of the tibial plateau, length of the lower limb (leg length), and location of the center of deformity (CD) were measured. Differences in the required correction angle at the hinge point between the two techniques (CAD) were compared, and correlation analysis was performed to reveal the influential factors. The mean difference in CAD between HBHTO and OWHTO was 0.78 ± 0.22 (0.4~1.5)°, and mean WBL position change per correction angle was 3.9 ± 0.3 (3.0~4.6)% in HBHTO and 4.1 ± 0.3 (3.1~4.7)% in OWHTO. Correlation analysis revealed a strong positive correlation between CAD and HKA. mLDFA, JLCA, MPTA, leg length, OWCD, HBCD, and HCD were also significantly correlated with CAD. HBHTO required a 5.6% larger correction angle at the hinge point to achieve the same amount of alignment correction as OWHTO. Full article

Progress in composite materials has led to their use in applications where improved mechanical and resistance characteristics are required. Most composites are obtained in such a way that they present specific mechanical properties and/or have the role of both a thermal conductor and insulator; these properties are important, specific, specialized, and useful. The advantages of these materials compared to the classic ones are as follows: low weight, high resistance to wear and corrosion, and mechanical characteristics consistent with the subsequent use of the product. The slightly high costs of these materials are justified by their precision, the quality of the products obtained, and the fact that their use leads to increased reliability, maintenance, and, in the cases of the automotive and aeronautical industries, reduced energy consumption. This paper aims to bring to readers’ attention the latest research related to glass fibre-reinforced composite materials in transport-related applications, such as automotive and aeronautic applications, including the manufacturing of unmanned aerial vehicles (UAVs). Considering the long period of use, the recycling and reuse of composite materials used in aeronautical transport is a must considering the environmental aspects and the need of achieving a circular economy. In recent years, considerable efforts have been made to find new alternatives to improve the performance and durability of materials in the aeronautical transport sector. Full article

Transformer has emerged as one of the modern neural networks that has been applied in numerous applications. However, transformers’ large and deep architecture makes them computationally and memory-intensive. In this paper, we propose the block g-circulant matrices to replace the dense weight matrices in the feedforward layers of the transformer and leverage the DCT-DST algorithm to multiply these matrices with the input vector. Our test using Portuguese-English datasets shows that the suggested method improves model memory efficiency compared to the dense transformer but at the cost of a slight drop in accuracy. We found that the model Dense-block 1-circulant DCT-DST of 128 dimensions achieved the highest model memory efficiency at 22.14%. We further show that the same model achieved a BLEU score of 26.47%. Full article

The permafrost in Northeast China is experiencing rapid degradation due to the influence of climate change and human activities, profoundly impacting the local ecological environment and engineering construction. Understanding the spatiotemporal dynamics of long-term permafrost in this region is crucial; however, systematic research on this topic remains scarce. This study combines meteorological station data, MODIS land surface temperature (LST) datasets, and borehole locations to apply the surface frost number (SFn) model. This approach enables the simulation and estimation of the spatial distribution and changes in the area of the surface frost number without vegetation effects (SFnv) and permafrost temperature (PT) in Northeast China from 1971 to 2020. The area of the SFnv > 0.49 within the permafrost region decreased substantially from approximately 44.353 × 10⁴ km² to 19.909 × 10⁴ km² between 1971 and 2020, with a notable change in 1988. The area of permafrost calculated using PT < 0 was slightly smaller, declining from 39.388 × 10⁴ km² to 29.852 × 10⁴ km². There was also a significant increase in the area with PT ranging from −1 °C to 0 °C, indicating a decline in permafrost stability. Approximately 10.926 × 10⁴ km² of stable permafrost has been transformed into semi-stable and unstable permafrost. Moreover, from 1982 to 2020, the NDVI was negatively correlated with the area of stable permafrost and positively correlated with the area of transitional or unstable permafrost. Vegetation cover decreased as transitional or unstable permafrost degraded. These findings provide valuable information for permafrost research and engineering development in cold regions, as well as for future planning and adaptation strategies. Full article

Allometry reflects the quantitative relationship between the allocation of resources among different organs. Understanding patterns of forest biomass allocation is critical to comprehending global climate change and the response of terrestrial vegetation to climate change. By collecting and reorganizing the existing allometric models of tree species in China, we established a database containing over 3000 empirical allometric models. Based on this database, we analyzed the model parameters and the effect of climate on forest biomass allocation under the context of ‘optimal allocation theory’. We showed that (1) the average and median exponent of power functions for above-ground biomass were 2.344 and 2.385, respectively, which significantly deviated from the theoretical prediction of 2.667 by metabolic theory (p < 0.01). (2) The parameters of the allometric model were not constant, and not significantly correlated with temperature, precipitation, latitude, and elevation (p > 0.05), but were more closely related to individual size (p < 0.01). (3) Among different types of forests, the proportion of above-ground biomass in tropical rainforests and subtropical evergreen rainforests was significantly higher than that in temperate forests and boreal forests (p < 0.05). The proportion of trunk and branch biomass allocated to tropical rainforest was significantly higher than that of boreal forest (p < 0.05), while the proportion of root and leaf biomass allocated to tropical rainforest was significantly lower than that of boreal forest (p < 0.05). (4) The abiotic environment plays a crucial role in determining the allocation of plant biomass. The ratio of below-ground/above-ground biomass is significantly and negatively correlated with both temperature and rainfall (p < 0.01), and significantly and positively correlated with altitude and latitude (p < 0.01). This means that as temperature and rainfall increase, there is a decrease in the amount of biomass allocated to below-ground structures such as roots. On the other hand, as altitude and latitude increase, there is an increase in below-ground biomass allocation. These findings highlight the importance of considering the influence of abiotic factors on plant growth and development. Full article

Infectious bursal disease virus (IBDV) is a significant burden for poultry production and market due to both direct disease and induced immunosuppression. In the present study, the expression of different cytokines in the bursa of Fabricius and thymus was evaluated during a 28-day-long experimental infection with two strains classified in the G1a (Classical) and G6 (ITA) genogroups. Although both strains significantly affected and modulated the expression of different molecules, the G6 strain seemed to induce a delayed immune response or suppress it more promptly. A recovery in the expression of several mediators was observed in the G1a-infected group at the end of the study, but not in the G6 one, further supporting a more persistent immunosuppression. This evidence fits with the higher replication level previously reported for the G6 and with the clinical outcome, as this genotype, although subclinical, has often been considered more immunosuppressive. However, unlike other studies focused on shorter time periods after infection, the patterns observed in this paper were highly variable and complex, depending on the strain, tissue, and time point, and characterized by a non-negligible within-group variability. Besides confirming the strain/genogroup effect on immune system modulation, the present study suggests the usefulness of longer monitoring activities after experimental infection to better understand the complex patterns and interactions with the host response. Full article

This comprehensive review delineates the extensive roles of Akkermansia muciniphila in various health domains, spanning from metabolic and inflammatory diseases to neurodegenerative disorders. A. muciniphila, known for its ability to reside in the mucous layer of the intestine, plays a pivotal role in maintaining gut integrity and interacting with host metabolic processes. Its influence extends to modulating immune responses and potentially easing symptoms across several non-communicable diseases, including obesity, diabetes, inflammatory bowel disease, and cancer. Recent studies highlight its capacity to interact with the gut–brain axis, suggesting a possible impact on neuropsychiatric conditions. Despite the promising therapeutic potential of A. muciniphila highlighted in animal and preliminary human studies, challenges remain in its practical application due to stability and cultivation issues. However, the development of pasteurized forms and synthetic mediums offers new avenues for its use in clinical settings, as recognized by regulatory bodies like the European Food Safety Authority. This narrative review serves as a crucial resource for understanding the broad implications of A. muciniphila across different health conditions and its potential integration into therapeutic strategies. Full article

Ginkgo biloba L. is a rare dioecious species that is valued for its diverse applications and is cultivated globally. This study aimed to develop a rapid and effective method for determining the sex of a Ginkgo biloba. Green and yellow leaves representing annual growth stages were scanned with a hyperspectral imager, and classification models for RGB images, spectral features, and a fusion of spectral and image features were established. Initially, a ResNet101 model classified the RGB dataset using the proportional scaling–background expansion preprocessing method, achieving an accuracy of 90.27%. Further, machine learning algorithms like support vector machine (SVM), linear discriminant analysis (LDA), and subspace discriminant analysis (SDA) were applied. Optimal results were achieved with SVM and SDA in the green leaf stage and LDA in the yellow leaf stage, with prediction accuracies of 87.35% and 98.85%, respectively. To fully utilize the optimal model, a two-stage Period-Predetermined (PP) method was proposed, and a fusion dataset was built using the spectral and image features. The overall accuracy for the prediction set was as high as 96.30%. This is the first study to establish a standard technique framework for Ginkgo sex classification using hyperspectral imaging, offering an efficient tool for industrial and ecological applications and the potential for classifying other dioecious plants. Full article

In this paper, different test cases where robotic arms are tested will be presented. A robotic arm is tested for the gravity effects that can be observed on it. The other robotic arm is tested for how much precision it has by using it to learn to write. The other robotic arm is tested on how well it can function as a solar tracker and how precisely it can function as an energy harvester. On the basis of these tests, the robotic arm’s mechanical structure, electronics, and software are put to the test. The software is based on evolutionary software that implements genetic algorithms. The entire command system is also ported to FPGAs (to hardware) to increase speed and response time. Full article

In recent years, cell migration assays (CMAs) have emerged as a tool to study the migration of cells along with their physiological responses under various stimuli, including both mechanical and bio-chemical properties. CMAs are a generic system in that they support various biological applications, such as wound healing assays. In this paper, we review the development of the CMA in the context of its application to wound healing assays. As such, the wound healing assay will be used to derive the requirements on CMAs. This paper will provide a comprehensive and critical review of the development of CMAs along with their application to wound healing assays. One salient feature of our methodology in this paper is the application of the so-called design thinking; namely we define the requirements of CMAs first and then take them as a benchmark for various developments of CMAs in the literature. The state-of-the-art CMAs are compared with this benchmark to derive the knowledge and technological gap with CMAs in the literature. We will also discuss future research directions for the CMA together with its application to wound healing assays. Full article

New β-amino-substituted porphyrin derivatives bearing carboxy groups were synthesized and their performance as sensitizers in dye-sensitized solar cells (DSSC) was evaluated. The new compounds were obtained in good yields (63–74%) through nucleophilic aromatic substitution reactions with 3-sulfanyl- and 4-sulfanylbenzoic acids. Although the electrochemical studies indicated suitable HOMO and LUMO energy levels for use in DSSC, the devices fabricated with these compounds revealed a low power conversion efficiency (PCE) that is primarily due to the low open-circuit voltage (V_oc) and short-circuit current density (J_sc) values. Full article

We report on the XPS analysis of degraded surfaces inside San Pietro Barisano, the rupestrian church carved into the calcarenite rock of ancient Matera, which has been a UNESCO World Heritage Site since 1993. As reported in previous works, the “Sassi” district and the park of rupestrian churches were available as open laboratories for the National Smart Cities SCN_00520 research project dedicated to the sustainable recovery of this remarkable architectural heritage. In that context, XPS functionality was shown to reside in the possibility of analyzing surfaces by feasible sampling, acquiring spectra without any preliminary sample treatment, and processing data using a well-established curve fitting procedure. The obtained results allowed us to identify the degradation products of the investigated surfaces, thus contributing to defining a diagnostic framework for subsequent actions. Accordingly, the samples here considered, collected from the internal wall surfaces of the church, were all analyzed in comparison with the reference calcarenite, and the XPS results were evaluated as a function of local environmental factors and the historical context of the church itself. The final aim was to provide, for each sample, the most representative indicator(s) of biotic and/or abiotic degradation for reliable use, in a multidisciplinary context, in planning care interventions for building heritage. Full article

Pulmonary metastasectomy has become a well-established procedure for patients with certain types of solid tumors. Patients are usually scheduled for staged lung metastasectomy in case of primary tumor control, the absence of distant non-lung metastases, and when complete resection is achievable. Nodules are removed with precision resection in order to ensure radical resection with minimal margins; this technique permits good oncological results, preserving the surrounding pulmonary parenchyma and causing minimal distortion compared to staplers. When possible, anatomical resections should be avoided since they are not justified by real oncological advantages and, in the majority of cases, sacrifice too much healthy tissue, possibly leading to inoperability in the case of metachronous relapses. Thus, preserving the maximum amount of pulmonary parenchyma is crucial because repeated metastasectomies are possible and frequent, with no theoretical limits to the number of reinterventions. In our multidisciplinary board team, we support the role of pulmonary metastasectomy as a useful curative therapy, with acceptable morbidity and mortality, with indications to be discussed case-by-case. Full article

Background and Objectives: Changes in activities of daily living (ADL) and quality of life (QOL) of patients with bone metastasis who underwent surgical treatment through Bone Metastasis Cancer Boards (BMCBs), a recent multidisciplinary approach for managing bone metastases, have been reported; however, no reports exist on patients who undergo conservative treatment. In this study, we aimed to evaluate these patients’ ADL and QOL and examine the factors influencing changes in these parameters. Materials and Methods: We retrospectively reviewed 200 patients with bone metastases who underwent conservative therapy through BMCBs between 2013 and 2021. A reassessment was conducted within 2–8 weeks after the initial assessment. Patients’ background and changes in performance status (PS), Barthel Index (BI), EuroQol five-dimension (EQ-5D) scores, and Numerical Rating Scale (NRS) scores were initially assessed. Furthermore, we categorized patients into two groups based on improvements or deteriorations in ADL and QOL and performed comparative analyses. Results: Significant improvements in EQ-5D (0.57 ± 0.02 versus [vs.] 0.64 ± 0.02), NRS max (5.21 ± 0.24 vs. 3.56 ± 0.21), and NRS average (2.98 ± 0.18 vs. 1.85 ± 0.13) scores were observed between the initial assessment and reassessment (all p < 0.001). PS (1.84 ± 0.08 vs. 1.72 ± 0.08) and BI (83.15 ± 1.68 vs. 84.42 ± 1.73) also showed improvements (p = 0.06, and 0.054, respectively). In addition, spinal cord paralysis (odds ratio [OR]: 3.69, p = 0.049; OR: 8.42, p < 0.001), chemotherapy (OR: 0.43, p = 0.02; OR: 0.25, p = 0.007), and NRS average scores (OR: 0.38, p = 0.02; OR: 0.14, p < 0.001) were independent factors associated with ADL and QOL. Conclusions: Patients with bone metastases who underwent conservative treatment through BMCBs exhibited an increase in QOL without a decline in ADL. The presence of spinal cord paralysis, absence of chemotherapy, and poor pain control were associated with a higher risk of deterioration in ADL and QOL. Full article

With abundant bandwidth resources, terahertz communications are considered one of the key technologies to meet the requirement for high data-rate transmission in the future. In order to compensate for the severe propagation loss of terahertz communications, directional antennas with high gain and narrow beams are expected to be adopted, making beam tracking significant for robust communications. In this paper, a tracking method based on power measurement is proposed, consisting of beam status monitoring, recognition of the deviation direction, and movement toward the optimal angle. By observing the change in the received signal power, beam misalignment is first checked, and whether the misalignment is out of tracking range is also determined. Then, the deviation direction is recognized by comparing the received power variations in the candidate directions, and the beam angle is adjusted accordingly until it reaches the optimal angle. With a small scanning range, the deviation direction is recognized in a short duration, allowing for rapid beam tracking. Numerical results indicate that the alignment error is competitively low and stable in the proposed beam tracking method, and its technical superiority is particularly dominant in situations involving variable motion at high speeds. Full article

Pertuzumab (Perjeta^®), a humanized antibody binding to the dimerization arm of HER2 (Human epidermal growth factor receptor-2), has failed as a monotherapy agent in HER2 overexpressing malignancies. Since the molecular interaction of HER2 with ligand-bound EGFR (epidermal growth factor receptor) has been implied in mitogenic signaling and malignant proliferation, we hypothesized that this interaction, rather than HER2 expression and oligomerization alone, could be a potential molecular target and predictor of the efficacy of pertuzumab treatment. Therefore, we investigated static and dynamic interactions between HER2 and EGFR molecules upon EGF stimulus in the presence and absence of pertuzumab in HER2+ EGFR+ SK-BR-3 breast tumor cells using Förster resonance energy transfer (FRET) microscopy and fluorescence correlation and cross-correlation spectroscopy (FCS/FCCS). The consequential activation of signaling and changes in cell proliferation were measured by Western blotting and MTT assay. The autocorrelation functions of HER2 diffusion were best fitted by a three-component model corrected for triplet formation, and among these components the slowly diffusing membrane component revealed aggregation induced by EGFR ligand binding, as evidenced by photon-counting histograms and co-diffusing fractions. This aggregation has efficiently been prevented by pertuzumab treatment, which also inhibited the post-stimulus interaction of EGFR and HER2, as monitored by changes in FRET efficiency. Overall, the data demonstrated that pertuzumab, by hindering post-stimulus interaction between EGFR and HER2, inhibits EGFR-evoked HER2 aggregation and phosphorylation and leads to a dose-dependent decrease in cell proliferation, particularly when higher amounts of EGF are present. Consequently, we propose that EGFR expression on HER2-positive tumors could be taken into consideration as a potential biomarker when predicting the outcome of pertuzumab treatment. Full article

To enhance the sustainability of microalgae-based swine wastewater treatment, this study aims to address the challenges of low efficiency in treating raw swine wastewater, collection difficulties, and high energy consumption treatment processes. The microalgae with strong environmental tolerance were first screened from swine wastewater, and its cultivation conditions were optimized to examine the effect of microalgae treatment on swine wastewater under optimal cultivation conditions. Additionally, the flocculation efficiency and mechanism of microalgae were analyzed. The results showed that Tetradesmus cf. obliquus ZYY1 exhibited the most robust heterotrophic growth. In the BG11 medium supplemented with glucose, the growth rate of T. cf. obliquus ZYY1 under chemoheterotrophic conditions was superior to its growth under photoheterotrophic conditions, reaching its peak with an optimal glucose concentration of 15 g/L. The biomass concentration of T. cf. obliquus ZYY1 in raw wastewater was significantly higher than that in sterilized wastewater, which reached 1.65 ± 0.01 g/L on the 10th day of treatment, with removal efficiencies of -N, -P, and the chemical oxygen demand reached 71.36%, 96.09%, and 93.13%, respectively. After raw wastewater treatment, the flocculation efficiency of T. cf. obliquus ZYY1 reached 97.71 ± 5.81%. This was attributed to the bacteria present in the raw wastewater, which induced T. cf. obliquus ZYY1 to secrete aromatic proteins. This study emphasizes the potential of microalgae as a green technology for sustainable wastewater treatment, offering a practical pathway for environmental protection and resource conservation. Full article

The mathematical model of a flexible multistate switch (FMSS) exhibits nonlinear and strong coupling characteristics, whereas traditional power decoupling control makes it difficult to completely decouple the output power. The traditional proportional–integral control parameters are difficult to adjust, and their robustness and dynamic performance are poor, which affects the stability of the voltage of the power distribution network and feeder power. To address these problems, this study first converted the original system into a linear system via coordinate transformation using feedback-accurate linearization to decouple active and reactive currents. Thereafter, a super-twisting sliding mode control (ST-SMC) algorithm was introduced, and an adaptive quasi-super-twisting sliding mode control (AQST-SMC) algorithm comprising the quasi-sliding mode function and adaptive proportional term was proposed. An FMSS double closed-loop controller was designed to achieve improved vibration suppression and convergence speed. A three-port FMSS simulation model was developed using MATLAB/Simulink, and the simulation results show that the proposed control strategy enhances the robustness and dynamic performance of the system. Full article

A multimodal mass spectrometry imaging (MSI) approach was used to investigate the chemotherapy drug-induced response of a Multicellular Tumour Spheroid (MCTS) 3D cell culture model of osteosarcoma (OS). The work addresses the critical demand for enhanced translatable early drug discovery approaches by demonstrating a robust spatially resolved molecular distribution analysis in tumour models following chemotherapeutic intervention. Advanced high-resolution techniques were employed, including desorption electrospray ionisation (DESI) mass spectrometry imaging (MSI), to assess the interplay between metabolic and cellular pathways in response to chemotherapeutic intervention. Endogenous metabolite distributions of the human OS tumour models were complemented with subcellularly resolved protein localisation by the detection of metal-tagged antibodies using Imaging Mass Cytometry (IMC). The first application of matrix-assisted laser desorption ionization–immunohistochemistry (MALDI-IHC) of 3D cell culture models is reported here. Protein localisation and expression following an acute dosage of the chemotherapy drug doxorubicin demonstrated novel indications for mechanisms of region-specific tumour survival and cell-cycle-specific drug-induced responses. Previously unknown doxorubicin-induced metabolite upregulation was revealed by DESI-MSI of MCTSs, which may be used to inform mechanisms of chemotherapeutic resistance. The demonstration of specific tumour survival mechanisms that are characteristic of those reported for in vivo tumours has underscored the increasing value of this approach as a tool to investigate drug resistance. Full article