BBX protein is a class of zinc finger transcription factors that have B-box domains at the N-terminus, and some of these proteins contain a CCT domain at the C-terminus. It plays an important role in plant growth, development, and metabolism. However, the expression
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BBX protein is a class of zinc finger transcription factors that have B-box domains at the N-terminus, and some of these proteins contain a CCT domain at the C-terminus. It plays an important role in plant growth, development, and metabolism. However, the expression pattern of BBX genes in alfalfa under hormonal and salt stresses is still unclear. In this study, we identified a total of 125
BBX gene family members by the available
Medicago reference genome in diploid alfalfa (
Medicago sativa spp.
Caerulea), a model plant (
M. truncatula), and tetraploid alfalfa (
M.
sativa), and divided these members into five subfamilies. We found that the conserved motifs of
BBXs of the same subfamily reveal similarities. We analyzed the collinearity relationship and duplication mode of these
BBX genes and found that the expression pattern of
BBX genes is specific in different tissues. Analysis of the available transcriptome data suggests that some members of the
BBX gene family are involved in multiple abiotic stress responses, and the highly expressed genes are often clustered together. Furthermore, we identified different expression patterns of some
BBX genes under salt, ethylene, salt and ethylene, salicylic acid, and salt and salicylic acid treatments, verified by qRT-PCR, and analyzed the subcellular localization of
MsBBX2,
MsBBX17, and
MsBBX32 using transient expression in tobacco. The results showed that
BBX genes were localized in the nucleus. This study systematically analyzed the
BBX gene family in
Medicago plants, which provides a basis for the study of
BBX gene family tolerance to abiotic stresses.
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