The 2023 MDPI Annual Report has
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16 pages, 301 KiB  
Article
A Pilot Study on Dietary Choices at Universities: Vending Machines, Canteens, and Lunch from Home
by Leandro Oliveira, Mona N. BinMowyna, Ibrahim Alasqah, Renata Puppin Zandonadi, Edite Teixeira-Lemos, Cláudia Chaves, Hmidan A. Alturki, Najla A. Albaridi, Fatmah Fahad Alribdi and António Raposo
Nutrients 2024, 16(11), 1722; https://doi.org/10.3390/nu16111722 (registering DOI) - 31 May 2024
Abstract
Commercial environments and food acquisition methods significantly shape dietary practices and impact health. This study assesses dietary choices among Portuguese university students regarding vending machines, canteens, and lunches from home. It also evaluates their use of the university canteen and their tendency to [...] Read more.
Commercial environments and food acquisition methods significantly shape dietary practices and impact health. This study assesses dietary choices among Portuguese university students regarding vending machines, canteens, and lunches from home. It also evaluates their use of the university canteen and their tendency to bring lunch from home. This pilot cross-sectional study used a self-administered electronic questionnaire, made available in early 2023. Participants were recruited through snowball sampling. The study included 137 students from Portuguese higher education institutions, mainly women (74.5%), pursuing degrees or integrated Master’s degrees (83.2%), primarily in health-related fields (55.5%). The median age was 21 years (20 to 23.5 years). About 70.0% regularly consumed food from vending machines, while approximately 60.0% brought lunch from home, avoiding the canteen. Factors such as convenience (48.5%), price (47.5%), product availability (40.6%), and taste (39.6%) mainly influenced vending machine choices. Monthly, chocolates, water, coffee, cookies, treats, and soft drinks were the most commonly acquired items, with coffee being the most frequent daily purchase. These findings provide insights for creating policies and initiatives to promote healthier and more accessible food options for students and strategies to encourage positive eating behaviors. Full article
(This article belongs to the Special Issue Public Health, Nutritional Behavior and Nutritional Status)
15 pages, 1064 KiB  
Review
Effects of Early and Late Time-Restricted Feeding on Parameters of Metabolic Health: An Explorative Literature Assessment
by Froso Petridi, Jan M. W. Geurts, Jean Nyakayiru, Anne Schaafsma, Dedmer Schaafsma, Ruth C. R. Meex and Cécile M. Singh-Povel
Nutrients 2024, 16(11), 1721; https://doi.org/10.3390/nu16111721 (registering DOI) - 31 May 2024
Abstract
Chrono-nutrition (meal timing) aligns food consumption with one’s circadian rhythm. The first meal (e.g., breakfast) likely promotes synchronization of peripheral circadian clocks, thereby supporting metabolic health. Time-restricted feeding (TRF) has been shown to reduce body weight (BW) and/or improve cardiovascular biomarkers. In this [...] Read more.
Chrono-nutrition (meal timing) aligns food consumption with one’s circadian rhythm. The first meal (e.g., breakfast) likely promotes synchronization of peripheral circadian clocks, thereby supporting metabolic health. Time-restricted feeding (TRF) has been shown to reduce body weight (BW) and/or improve cardiovascular biomarkers. In this explorative literature assessment, 13 TRF randomized controlled trials (RCTs) were selected from PubMed and Scopus to evaluate the effects of early (eTRF: first meal before 10:30 a.m.) and late TRF (lTRF: first meal after 11:30 a.m.) on parameters of metabolic health. Although distinct variations in study design were evident between reports, TRF consistently decreased energy intake (EI) and BW, and improved insulin resistance as well as systolic blood pressure. eTRF seemed to have a greater beneficial effect than lTRF on insulin resistance (HOMA-IR). Importantly, most studies did not appear to consider chronotype in their evaluation, which may have underestimated TRF effects. TRF intervention may be a promising approach for risk reduction of human metabolic diseases. To conclusively determine benefits of TRF and identify clear differences between eTRF and lTRF, future studies should be longer-term (≥8 weeks) with well-defined (differences in) feeding windows, include participants chronotypically matching the intervention, and compare outcomes to those of control groups without any dietary limitations. Full article
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20 pages, 7942 KiB  
Article
Interannual Variability of Water and Heat Fluxes in a Woodland Savanna (Cerrado) in Southeastern Brazil: Effects of Severe Drought and Soil Moisture
by Lucas F. C. da Conceição, Humberto R. da Rocha, Nelson V. Navarrete, Rafael Rosolem, Osvaldo M. R. Cabral and Helber C. de Freitas
Atmosphere 2024, 15(6), 668; https://doi.org/10.3390/atmos15060668 (registering DOI) - 31 May 2024
Abstract
The Brazilian Cerrado biome is known for its high biodiversity, and the role of groundwater recharge and climate regulation. Anthropogenic influence has harmed the biome, emphasizing the need for science to understand its response to climate and reconcile economic exploration with preservation. Our [...] Read more.
The Brazilian Cerrado biome is known for its high biodiversity, and the role of groundwater recharge and climate regulation. Anthropogenic influence has harmed the biome, emphasizing the need for science to understand its response to climate and reconcile economic exploration with preservation. Our work aimed to evaluate the seasonal and interannual variability of the surface energy balance in a woodland savanna (Cerrado) ecosystem in southeastern Brazil over a period of 19 years, from 2001 to 2019. Using field micrometeorological measurements, we examined the variation in soil moisture and studied its impact on the temporal pattern of energy fluxes to distinguish the effects during rainy years compared to a severe drought spell. The soil moisture measures used two independent instruments, cosmic ray neutron sensor CRNS, and FDR at different depths. The measures were taken at the Pé de Gigante (PEG) site, in a region of well-defined seasonality with the dry season in winter and a hot/humid season in summer. We gap-filled the energy flux measurements with a calibrated biophysical model (SiB2). The long-term averages for air temperature and precipitation were 22.5 °C and 1309 mm/year, respectively. The net radiation (Rn) was 142 W/m2, the evapotranspiration (ET) and sensible heat flux (H) were 3.4 mm/d and 52 W/m2, respectively. Soil moisture was marked by a pronounced negative anomaly in the 2014 year, which caused an increase in the Bowen ratio and a decrease in Evaporative fraction, that lasted until the following year 2015 during the dry season, despite the severe meteorological drought of 2013/2014 already ending, which was corroborated by the two independent measurements. The results showed the remarkable influence of precipitation and soil moisture on the interannual variability of the energy balance in this Cerrado ecosystem, aiding in understanding how it responds to strong climate disturbances. Full article
(This article belongs to the Special Issue Land-Atmosphere Interactions)
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17 pages, 3873 KiB  
Article
Effect of Hydroxyurea on Morphology, Proliferation, and Protein Expression on Taenia crassiceps WFU Strain
by Diana G. Rios-Valencia, Karel Estrada, Arturo Calderón-Gallegos, Rocío Tirado-Mendoza, Raúl J. Bobes, Juan P. Laclette and Margarita Cabrera-Bravo
Int. J. Mol. Sci. 2024, 25(11), 6061; https://doi.org/10.3390/ijms25116061 (registering DOI) - 31 May 2024
Abstract
Flatworms are known for their remarkable regenerative ability, one which depends on totipotent cells known as germinative cells in cestodes. Depletion of germinative cells with hydroxyurea (HU) affects the regeneration of the parasite. Here, we studied the reduction and recovery of germinative cells [...] Read more.
Flatworms are known for their remarkable regenerative ability, one which depends on totipotent cells known as germinative cells in cestodes. Depletion of germinative cells with hydroxyurea (HU) affects the regeneration of the parasite. Here, we studied the reduction and recovery of germinative cells in T. crassiceps cysticerci after HU treatment (25 mM and 40 mM of HU for 6 days) through in vitro assays. Viability and morphological changes were evaluated. The recovery of cysticerci’s mobility and morphology was evaluated at 3 and 6 days, after 6 days of treatment. The number of proliferative cells was evaluated using EdU. Our results show morphological changes in the size, shape, and number of evaginated cysticerci at the 40 mM dose. The mobility of cysticerci was lower after 6 days of HU treatment at both concentrations. On days 3 and 6 of recovery after 25 mM of HU treatment, a partial recovery of the proliferative cells was observed. Proteomic and Gene Ontology analyses identified modifications in protein groups related to DNA binding, DNA damage, glycolytic enzymes, cytoskeleton, skeletal muscle, and RNA binding. Full article
(This article belongs to the Special Issue Advances in Therapeutics against Eukaryotic Pathogens)
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14 pages, 2520 KiB  
Article
Neuron Circuit Based on a Split-gate Transistor with Nonvolatile Memory for Homeostatic Functions of Biological Neurons
by Hansol Kim, Sung Yun Woo and Hyungjin Kim
Biomimetics 2024, 9(6), 335; https://doi.org/10.3390/biomimetics9060335 (registering DOI) - 31 May 2024
Abstract
To mimic the homeostatic functionality of biological neurons, a split-gate field-effect transistor (S-G FET) with a charge trap layer is proposed within a neuron circuit. By adjusting the number of charges trapped in the Si3N4 layer, the threshold voltage (V [...] Read more.
To mimic the homeostatic functionality of biological neurons, a split-gate field-effect transistor (S-G FET) with a charge trap layer is proposed within a neuron circuit. By adjusting the number of charges trapped in the Si3N4 layer, the threshold voltage (Vth) of the S-G FET changes. To prevent degradation of the gate dielectric due to program/erase pulses, the gates for read operation and Vth control were separated through the fin structure. A circuit that modulates the width and amplitude of the pulse was constructed to generate a Program/Erase pulse for the S-G FET as the output pulse of the neuron circuit. By adjusting the Vth of the neuron circuit, the firing rate can be lowered by increasing the Vth of the neuron circuit with a high firing rate. To verify the performance of the neural network based on S-G FET, a simulation of online unsupervised learning and classification in a 2-layer SNN is performed. The results show that the recognition rate was improved by 8% by increasing the threshold of the neuron circuit fired. Full article
(This article belongs to the Special Issue New Insights into Bio-Inspired Neural Networks)
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26 pages, 5057 KiB  
Article
Assessing the Belt and Road Initiative’s Impact: A Multi-Regression Model Based on Economic Interaction
by Tingsong Wang, Jingyi Xu, Yong Jin and Shuaian Wang
Sustainability 2024, 16(11), 4694; https://doi.org/10.3390/su16114694 (registering DOI) - 31 May 2024
Abstract
This study examines the impact of joining the Belt and Road Initiative (BRI) on the economies of ASEAN countries, focusing on the shipping industry’s performance. Ten economic interaction indicators were analyzed using data from 2015–2022 and predicting future data for 2015–2030 through GM(1,1) [...] Read more.
This study examines the impact of joining the Belt and Road Initiative (BRI) on the economies of ASEAN countries, focusing on the shipping industry’s performance. Ten economic interaction indicators were analyzed using data from 2015–2022 and predicting future data for 2015–2030 through GM(1,1) and FOA-SVR models. The principal component regression (PCR) model, combined with the Analytic Hierarchy Process (AHP), assessed the correlation of these indicators with GDP and port container throughput (PCT). The findings reveal a strong correlation between economic interaction scores with China and economic and shipping performance, highlighting Chinese investment’s significant impact on GDP and shipping connectivity’s substantial influence on container throughput. This study provides a framework for quantifying organizational engagement levels and policy effectiveness. Full article
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17 pages, 1554 KiB  
Article
Coopetition with the Industrial IoT: A Service-Dominant Logic Approach
by Agostinho da Silva and Antonio J. Marques Cardoso
Appl. Syst. Innov. 2024, 7(3), 47; https://doi.org/10.3390/asi7030047 - 31 May 2024
Abstract
Abstract: This research addresses the critical gap in enabling effective coopetition networks through technological innovation with the development of Cockpit4.0+, an Industrial Internet of Things (IIoT) artefact tailored for small- and medium-sized enterprises (SMEs). By employing the principles of Service-Dominant Logic (S-D Logic) [...] Read more.
Abstract: This research addresses the critical gap in enabling effective coopetition networks through technological innovation with the development of Cockpit4.0+, an Industrial Internet of Things (IIoT) artefact tailored for small- and medium-sized enterprises (SMEs). By employing the principles of Service-Dominant Logic (S-D Logic) and leveraging the Design Science Research (DSR) methodology, Cockpit4.0+ represents a pioneering approach to incorporating the IIoT within ecosystems for value co-creation. This facilitates competition and cooperation among firms, enhancing the operational dynamics within SME networks. Evaluated by experts in the ornamental stone sector, a significant sector of the Portuguese economy, the system demonstrated a positive functional acceptance rate of 78.9%. An experimental test was conducted following the positive preliminary functional evaluation of Cockpit4.0+, especially among more digitally advanced companies. The findings revealed that the on-time delivery performance under current best practices (CB.Ps) was 67.1%. In contrast, implementing coopetition network practices (CN.Ps) increased on-time delivery to 77.5%. These positive evaluations of Cockpit4.0+ underscore the practical applicability of S-D Logic and provide fresh insights into the dynamics of coopetition, particularly beneficial for SMEs. Despite its promising results, the real-world efficacy of IIoT systems like Cockpit4.0+ requires further empirical studies to verify these findings. Future research should focus on examining the scalability of Cockpit4.0+ and its adaptability across various sectors and enhancing its cybersecurity measures to ensure its long-term success and broader adoption. Full article
(This article belongs to the Special Issue New Challenges of Innovation, Sustainability, Resilience in X.0 Era)
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17 pages, 5541 KiB  
Article
Experimental Characterization of Fabric-Reinforced Cementitious Matrix (FRCM) Systems Applied on Calcarenite Stone: Adoption of Non-Standard Setup for Double-Shear Bond Tests
by Maria Concetta Oddo, Liborio Cavaleri, Catherine Papanicolaou and Lidia La Mendola
J. Compos. Sci. 2024, 8(6), 206; https://doi.org/10.3390/jcs8060206 - 31 May 2024
Abstract
The use of Fabric-Reinforced Cementitious Matrix (FRCM) systems is an innovative method for strengthening structures, particularly masonry, while addressing environmental and economic concerns. Despite their widespread use, characterizing FRCM composites poses challenges due to their complex mechanical behavior and considerable variability in properties. [...] Read more.
The use of Fabric-Reinforced Cementitious Matrix (FRCM) systems is an innovative method for strengthening structures, particularly masonry, while addressing environmental and economic concerns. Despite their widespread use, characterizing FRCM composites poses challenges due to their complex mechanical behavior and considerable variability in properties. The available standardized testing methods exhibit some inconsistencies, underscoring the need for reliable characterization procedures. This paper presents an experimental study on the bond behavior between FRCM materials and calcarenite stone using a non-standard setup for double shear bond tests. Different FRCM systems are considered, varying the matrix composition and fabric nature. The experimental results are evaluated in terms of maximum stress, slip and data dispersion, alongside comparisons with double shear tests on larger samples and single-lap shear. These findings provide insights into how the mortar nature influences the stress-slip curves, strength, ductility and failure modes. The experimental study demonstrates the repeatability and robustness, particularly in terms of peak strength, of the non-standard setup configuration utilized in the study. The study highlights the importance of reliable characterization procedures for FRCM materials, especially in bond behavior assessments, emphasizing the need for further research to enhance our understanding of their application in structural reinforcement. Full article
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19 pages, 4600 KiB  
Article
An Enhanced Tree-Seed Algorithm for Function Optimization and Production Optimization
by Qingan Zhou, Rong Dai, Guoxiao Zhou, Shenghui Ma and Shunshe Luo
Biomimetics 2024, 9(6), 334; https://doi.org/10.3390/biomimetics9060334 - 31 May 2024
Abstract
As the fields of engineering, energy, and geology become increasingly complex, decision makers face escalating challenges that require skilled solutions to meet practical production needs. Evolutionary algorithms, inspired by biological evolution, have emerged as powerful methods for tackling intricate optimization problems without relying [...] Read more.
As the fields of engineering, energy, and geology become increasingly complex, decision makers face escalating challenges that require skilled solutions to meet practical production needs. Evolutionary algorithms, inspired by biological evolution, have emerged as powerful methods for tackling intricate optimization problems without relying on gradient data. Among these, the tree-seed algorithm (TSA) distinguishes itself due to its unique mechanism and efficient searching capabilities. However, an imbalance between its exploitation and exploration phases can lead it to be stuck in local optima, impeding the discovery of globally optimal solutions. This study introduces an improved TSA that incorporates water-cycling and quantum rotation-gate mechanisms. These enhancements assist the algorithm in escaping local peaks and achieving a more harmonious balance between its exploitation and exploration phases. Comparative experimental evaluations, using the CEC 2017 benchmarks and a well-known metaheuristic algorithm, demonstrate the upgraded algorithm’s faster convergence rate and enhanced ability to locate global optima. Additionally, its application in optimizing reservoir production models underscores its superior performance compared to competing methods, further validating its real-world optimization capabilities. Full article
(This article belongs to the Special Issue Computer-Aided Biomimetics: 2nd Edition)
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15 pages, 2716 KiB  
Article
Elucidation of Shoot and Root Growth, Physiological Responses, and Quality Traits of Tomato (Solanum lycopersicon L.) Exposed to Elevated Calcium Carbonate Concentrations
by Naveedullah Sediqui, Mohammad Wasif Amin, Niamatullah Dawlatzai, Gulbuddin Gulab, Dawlat Shah Poyesh, Naoki Terada, Atsushi Sanada, Atsushi Kamata and Kaihei Koshio
Horticulturae 2024, 10(6), 573; https://doi.org/10.3390/horticulturae10060573 - 31 May 2024
Abstract
The excess presence of calcium carbonate (CaCO3) in soil poses challenges for production of horticultural crops, including tomatoes. This condition is prevalent in arid and semi-arid regions of Afghanistan. The objective of this study was to evaluate the effects of elevated [...] Read more.
The excess presence of calcium carbonate (CaCO3) in soil poses challenges for production of horticultural crops, including tomatoes. This condition is prevalent in arid and semi-arid regions of Afghanistan. The objective of this study was to evaluate the effects of elevated concentrations of CaCO3 on growth, physiology, and quality attributes of tomato. Seedlings were exposed to different concentrations of CaCO3 (0%, 2.5%, 5%, 10%, and 20% w/w) in soil. The results showed that elevated concentrations of CaCO3 (10% and 20%) significantly increased soil electrical conductivity (EC) and pH, and subsequently affected growth, physiology, and quality of tomato. CaCO3 effects resulted in an increase in leaf electrolyte leakage, leaf calcium content, root respiration rate, root ethylene production, fruit firmness, total soluble solids, ascorbic acid, and organic acids, as well as a decrease in plant height, leaf length, leaf magnesium content, leaf SPAD value, number of leaves per plant, root weight and length, and root activity. At higher concentrations, CaCO3 decreased number of flowers and fruit per plant, as well as fruit weight and diameter, consequently affecting yield production. Although elevated concentrations of CaCO3 is characteristic of soils in Afghanistan, limited information is available about this topic. These findings enhance our understanding of soil conditions in the country and provide valuable insights for farmers. Full article
(This article belongs to the Section Fruit Production Systems)
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16 pages, 8278 KiB  
Article
Ag Nanoparticles Deposited onto BaTiO3 Aerogel for Highly Efficient Photodegradation
by Jun Wu, Wen Yan, Mengyuan Xie, Kai Zhong, Sheng Cui and Xiaodong Shen
Gels 2024, 10(6), 378; https://doi.org/10.3390/gels10060378 - 31 May 2024
Abstract
Given the increasingly severe environmental problems caused by water pollution, the degradation of organic dyes can be effectively achieved through the utilization of photocatalysis. In this work, metal alkoxides and a combination of alcohol/hydrophobic solvents are employed to prepare BaTiO3 aerogels via [...] Read more.
Given the increasingly severe environmental problems caused by water pollution, the degradation of organic dyes can be effectively achieved through the utilization of photocatalysis. In this work, metal alkoxides and a combination of alcohol/hydrophobic solvents are employed to prepare BaTiO3 aerogels via a liquid-phase and template-free synthetic route. The preparation process of the aerogels solely entails facile agitation and supercritical drying, eliminating the need for additional heat treatment. The binary solvent of ethanol and toluene is identified as the optimal choice, resulting in a significantly enhanced surface area (up to 223 m2/g) and an abundant pore structure of BaTiO3 aerogels compared to that of the BaTiO3 nanoparticles. Thus, the removal efficiency of the BaTiO3 aerogel sample for MO is nearly twice as high as that of the BaTiO3 nanoparticles sample. Noble metal Ag nanoparticles’ deposition onto the BaTiO3 aerogel surface is further achieved via the photochemical deposition method, which enhances the capture of photogenerated electrons, thereby ensuring an elevated level of photocatalytic efficiency. As a result, Ag nanoparticles deposited on BaTiO3 aerogel can degrade MO completely after 40 min of illumination, while the corresponding aerogel before modification can only remove 80% of MO after 60 min. The present work not only complements the preparatory investigation of intricate aerogels but also offers a fresh perspective for the development of diverse perovskite aerogels with broad applications. Full article
(This article belongs to the Special Issue Preparation and Characteristics of Aerogel-Based Materials)
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14 pages, 1621 KiB  
Article
Cationic Glucan Dendrimer Gel-Mediated Local Delivery of Anti-OC-STAMP-siRNA for Treatment of Pathogenic Bone Resorption
by Kenta Yamamoto, Shin-Ichi Sawada, Satoru Shindo, Shin Nakamura, Young M. Kwon, Nazanin Kianinejad, Saynur Vardar, Maria Hernandez, Kazunari Akiyoshi and Toshihisa Kawai
Gels 2024, 10(6), 377; https://doi.org/10.3390/gels10060377 - 31 May 2024
Abstract
Osteoclast stimulatory transmembrane protein (OC-STAMP) plays a pivotal role in the promotion of cell fusion during osteoclast differentiation (osteoclastogenesis) in the context of pathogenic bone resorption. Thus, it is plausible that the suppression of OC-STAMP through a bioengineering approach could lead to the [...] Read more.
Osteoclast stimulatory transmembrane protein (OC-STAMP) plays a pivotal role in the promotion of cell fusion during osteoclast differentiation (osteoclastogenesis) in the context of pathogenic bone resorption. Thus, it is plausible that the suppression of OC-STAMP through a bioengineering approach could lead to the development of an effective treatment for inflammatory bone resorptive diseases with minimum side effects. Here, we synthesized two types of spermine-bearing (Spe) cationic glucan dendrimer (GD) gels (with or without C12) as carriers of short interfering RNA (siRNA) to silence OC-STAMP. The results showed that amphiphilic C12-GD-Spe gel was more efficient in silencing OC-STAMP than GD-Spe gel and that the mixture of anti-OC-STAMP siRNA/C12-GD-Spe significantly downregulated RANKL-induced osteoclastogenesis. Also, local injection of anti-OC-STAMP-siRNA/C12-GD-Spe could attenuate bone resorption induced in a mouse model of periodontitis. These results suggest that OC-STAMP is a promising target for the development of a novel bone regenerative therapy and that C12-GD-Spe gel provides a new nanocarrier platform of gene therapies for osteolytic disease. Full article
(This article belongs to the Section Gel Applications)
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16 pages, 5382 KiB  
Article
Genome-Wide Identification of SNARE Family Genes and Functional Characterization of an R-SNARE Gene BbSEC22 in a Fungal Insect Pathogen Beauveria bassiana
by Fang Li, Juefeng Zhang, Haiying Zhong, Kaili Yu and Jianming Chen
J. Fungi 2024, 10(6), 393; https://doi.org/10.3390/jof10060393 - 31 May 2024
Abstract
Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) are central components of the machinery mediating cell membrane fusion and intracellular vesicular trafficking in eukaryotic cells, and have been well-documented to play critical roles in growth, development, and pathogenesis in the filamentous fungal plant pathogens. [...] Read more.
Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) are central components of the machinery mediating cell membrane fusion and intracellular vesicular trafficking in eukaryotic cells, and have been well-documented to play critical roles in growth, development, and pathogenesis in the filamentous fungal plant pathogens. However, little is known about the contributions of SNAREs to the physiology and biocontrol potential in entomopathogenic filamentous fungi. Here, a genome-wide analysis of SNARE genes was performed taking advantage of the available whole genome sequence of Beauveria bassiana, a classical entomopathogenic fungus. Based on the compared genomic method, 22 genes encoding putative SNAREs were identified from the whole genome of B. bassiana, and were classified into four groups (7 Qa-, 4 Qb-, 6 Qc-, and 5 R-SNAREs) according to the conserved structural features of their encoding proteins. An R-SNARE encoding gene BbSEC22 was further functionally characterized by gene disruption and complementation. The BbSEC22 null mutant showed a fluffy appearance in mycelial growth and an obvious lag in conidial germination. The null mutant also exhibited significantly increased sensitivity to oxidative stress and cell wall perturbing agents and reduced the yield of conidia production by 43.1% compared with the wild-type strain. Moreover, disruption of BbSEC22 caused a significant decrease in conidial virulence to Spodoptera litura larvae. Overall, our results provide an overview of vesicle trafficking in B. bassiana and revealed that BbSec22 was a multifunctional protein associated with mycelial growth, sporulation, conidial germination, stress tolerance, and insecticidal virulence. Full article
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14 pages, 1489 KiB  
Article
Chemical and Energetic Characterization of the Wood of Prosopis laevigata: Chemical and Thermogravimetric Methods
by Luis Fernando Pintor-Ibarra, José Juan Alvarado-Flores, José Guadalupe Rutiaga-Quiñones, Jorge Víctor Alcaraz-Vera, María Liliana Ávalos-Rodríguez and Oswaldo Moreno-Anguiano
Molecules 2024, 29(11), 2587; https://doi.org/10.3390/molecules29112587 (registering DOI) - 31 May 2024
Abstract
Diverse methodologies exist to determine the chemical composition, proximate analysis, and calorific value of biomass. Researchers select and apply a specific methodology according to the lignocellulosic material they study and the budgetary resources available. In this project, we determined the primary chemical constitution [...] Read more.
Diverse methodologies exist to determine the chemical composition, proximate analysis, and calorific value of biomass. Researchers select and apply a specific methodology according to the lignocellulosic material they study and the budgetary resources available. In this project, we determined the primary chemical constitution and proximate analysis of Prosopis laevigata (Humb. & Bonpl.) Jonhst wood using a traditional chemical method and a novel procedure based on the deconvolution of the DTG signal produced by TGA. The highest calorific value was verified using a calorimetric pump based on mathematical models. We also conducted elemental analysis and a microanalysis of ash, and applied Fourier transform infrared spectroscopic analysis (FT-IR). The means of the results obtained by the chemical method and TGA-DTG, respectively, were: hemicelluloses 7.36%–(8.72%), cellulose 48.28%–(46.08%), lignin 30.57%–(32.44%), extractables 13.53%–(12.72%), moisture 2.03%–(4.96%), ash 1.77%–(1.90%), volatile matter 75.16%–(74.14%), and fixed carbon 23.05%–(18.93%). The procedure with the calorimetric pump generated a calorific value above 20.16 MJ/kg. The range generated by the various models was 18.23–21.07 MJ/kg. The results of the elemental analysis were: carbon 46.4%, hydrogen 6.79%, oxygen 46.43%, nitrogen 0.3%, and sulfur 0.5%. The microanalysis of ash identified 18 elements. The most abundant ones were potassium ˃ calcium ˃ sodium. Based on the infrared spectrum (FT-IR) of Prosopis laevigata wood, we detected the following functional groups: OH, C-H, C=O, CH2, CH3, C-O-C, C-OH, and C4-OH. Our conclusion is that the TGA-DTG method made it possible to obtain results in less time with no need for the numerous reagents that chemical procedures require. The calorific value of P. laevigata wood is higher than the standards. Finally, according to our results, proximate analysis provides the best model for calculating calorific value. Full article
(This article belongs to the Special Issue Lignocellulosic Biomass III)
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14 pages, 285 KiB  
Article
Feeding a Multi-Enzyme Blend to Enhance the Nutrient Digestibility of Wheat-Canola Expeller Diets in Ileal-Cannulated Weaned Pigs
by A. Janine Soderstrom, Li Fang Wang, Rob Patterson, Eduardo Beltranena and Ruurd T. Zijlstra
Animals 2024, 14(11), 1644; https://doi.org/10.3390/ani14111644 (registering DOI) - 31 May 2024
Abstract
Canola expeller (CE) contains ~200 g/kg residual oil, but also fiber that impairs nutrient digestibility in weaned pigs. To study if feed enzymes increase digestibility, six diets containing either the basal or two CE samples mixed in at 250 g/kg (CE-A or CE-B) [...] Read more.
Canola expeller (CE) contains ~200 g/kg residual oil, but also fiber that impairs nutrient digestibility in weaned pigs. To study if feed enzymes increase digestibility, six diets containing either the basal or two CE samples mixed in at 250 g/kg (CE-A or CE-B) were formulated with or without a multi-enzyme blend containing cellulase, xylanase, glucanase, amylase, protease, invertase, and pectinase. The basal diet containing 620 g/kg wheat and 150 g/kg barley served as control. Twelve ileal-cannulated barrows (9–15 kg) were fed the six diets in a replicated 6 (pigs) × 3 (periods) Youden square. Ileal digestibility of gross energy and amino acids was 5% greater for basal than CE diets without differences between CE samples. Diet energy values were 4% greater for CE than basal diets due to residual oil in CE. Inclusion of the multi-enzyme blend increased total tract digestibility of energy of the basal but not CE diets by 2%. Net energy value was greater for CE-A than CE-B because CE-A contained more residual oil. In conclusion, feeding 250 g/kg CE increased diet energy values; thus, CE can substitute added fat in weaned pig diets. Feeding the multi-enzyme blend increased the energy digestibility of wheat and barley-based diets fed to weaned pigs. However, research is needed to identify enzyme combinations that increase the nutrient digestibility of CE. Full article
(This article belongs to the Section Animal Nutrition)
16 pages, 4096 KiB  
Article
Biotransformation of Chlorpyrifos Shewanella oneidensis MR-1 in the Presence of Goethite: Experimental Optimization and Degradation Products
by Shen Tang, Yanhong Li, Zongqiang Zhu, Yaru Wang, Yuqing Peng, Jing Zhang, Peijie Nong, Shufen Pan, Yinming Fan and Yinian Zhu
Toxics 2024, 12(6), 402; https://doi.org/10.3390/toxics12060402 - 31 May 2024
Abstract
In this study, the degradation system of Shewanella oneidensis MR-1 and goethite was constructed with chlorpyrifos as the target contaminant. The effects of initial pH, contaminant concentration, and temperature on the removal rate of chlorpyrifos during the degradation process were investigated. The experimental [...] Read more.
In this study, the degradation system of Shewanella oneidensis MR-1 and goethite was constructed with chlorpyrifos as the target contaminant. The effects of initial pH, contaminant concentration, and temperature on the removal rate of chlorpyrifos during the degradation process were investigated. The experimental conditions were optimized by response surface methodology with a Box–Behnken design (BBD). The results show that the removal rate of chlorpyrifos is 75.71% at pH = 6.86, an initial concentration of 19.18 mg·L−1, and a temperature of 30.71 °C. LC-MS/MS analyses showed that the degradation products were C4H11O3PS, C7H7Cl3NO4P, C9H11Cl2NO3PS, C7H7Cl3NO3PS, C9H11Cl3NO4P, C4H11O2PS, and C5H2Cl3NO. Presumably, the degradation pathways involved are: enzymatic degradation, hydrolysis, dealkylation, desulfur hydrolysis, and dechlorination. The findings of this study demonstrate the efficacy of the goethite/S. oneidensis MR-1 complex system in the removal of chlorpyrifos from water. Consequently, this research contributes to the establishment of a theoretical framework for the microbial remediation of organophosphorus pesticides in aqueous environments. Full article
(This article belongs to the Topic Removal of Hazardous Substances from Water Resources)
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12 pages, 1423 KiB  
Article
Influence of Heating Temperature and pH on Acid Gelation of Micellar Calcium Phosphate-Adjusted Skim Milk
by Elaheh Ahmadi, Todor Vasiljevic and Thom Huppertz
Foods 2024, 13(11), 1724; https://doi.org/10.3390/foods13111724 - 31 May 2024
Abstract
Micellar calcium phosphate (MCP) plays an important role in maintaining the structure and stability of the casein micelle and its properties during processing. The objective of this study was to investigate how heating (10 min at 80 or 90 °C) at different pH [...] Read more.
Micellar calcium phosphate (MCP) plays an important role in maintaining the structure and stability of the casein micelle and its properties during processing. The objective of this study was to investigate how heating (10 min at 80 or 90 °C) at different pH levels (6.3, 6.6, 6.9, or 7.2) impacted the acid-induced gelation of MCP-adjusted milk, containing 67 (MCP67), 100 (MCP100), or 113 (MCP113) % of the original MCP content. The unheated sample MCP100 at pH 6.6 was considered the control. pH acidification to pH 4.5 at 30 °C was achieved with glucono delta-lactone while monitoring viscoelastic behaviour by small-amplitude oscillatory rheology. The partitioning of calcium and proteins between colloidal and soluble phases was also examined. In MCP-depleted skim milk samples, the concentrations of non-sedimentable caseins and whey proteins were higher compared to the control and MCP-enriched skim milk samples. The influence of MCP adjustment on gelation was dependent on pH. Acid gels from sample MCP67 exhibited the highest storage modulus (G′). At other pH levels, MCP100 resulted in the greatest G′. The pH of MCP-adjusted skim milk also impacted the gel properties after heating. Overall, this study highlights the substantial impact of MCP content on the acid gelation of milk, with a pronounced dependency of the MCP adjustment effect on pH variations. Full article
(This article belongs to the Section Dairy)
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9 pages, 2040 KiB  
Communication
The Use of Ultraviolet Irradiation to Improve the Efficacy of Acids That Are Generally Recognized as Safe for Disinfecting Fresh Produce in the Ready-to-Eat Stage
by Ruxianguli Maimaitiyiming, Yuting Yang, Ailikemu Mulati, Aihemaitijiang Aihaiti and Jiayi Wang
Foods 2024, 13(11), 1723; https://doi.org/10.3390/foods13111723 - 31 May 2024
Abstract
Fresh-cut produce is usually produced under standardized disinfection processes, which are unavailable at the ready-to-eat stage. Currently, chemical sanitizers are used for washing, but their disinfection efficacy is limited. In this study, UV-C (1.03 kJ/m2) was combined with organic acids that [...] Read more.
Fresh-cut produce is usually produced under standardized disinfection processes, which are unavailable at the ready-to-eat stage. Currently, chemical sanitizers are used for washing, but their disinfection efficacy is limited. In this study, UV-C (1.03 kJ/m2) was combined with organic acids that are generally recognized as safe (GRAS), including citric, malic, acetic, and lactic acids (LAs), to wash lettuce and cherry tomatoes that are contaminated with Escherichia coli O157:H7 and Salmonella Typhimurium. The results showed that LA was the most effective treatment among the single treatments, with a pathogen reduction and cross-contamination incidence of 2.0–2.3 log CFU/g and 28–35%, respectively. After combining with UV-C, the disinfection efficacy and cross-contamination prevention capacity of the four GRAS acids significantly improved. Among the combination treatments, the highest pathogen reduction (2.5–2.7 log CFU/g) and the lowest cross-contamination incidence (11–15%) were achieved by LA-UV. The analyses of ascorbic acid, chlorophyll, lycopene, antioxidant capacity, and ΔE indicated that neither the single nor combination treatments negatively affected the quality properties. These results provide a potential hurdle technology for fresh produce safety improvement at the ready-to-eat stage. Full article
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15 pages, 2639 KiB  
Article
Assessment of Peanut Protein Powder Quality by Near-Infrared Spectroscopy and Generalized Regression Neural Network-Based Approach
by Haofan Cui, Fengying Gu, Jingjing Qin, Zhenyuan Li, Yu Zhang, Qin Guo and Qiang Wang
Foods 2024, 13(11), 1722; https://doi.org/10.3390/foods13111722 - 31 May 2024
Abstract
The global demand for protein is on an upward trajectory, and peanut protein powder has emerged as a significant player, owing to its affordability and high quality, with great future market potential. However, the industry currently lacks efficient methods for rapid quality testing. [...] Read more.
The global demand for protein is on an upward trajectory, and peanut protein powder has emerged as a significant player, owing to its affordability and high quality, with great future market potential. However, the industry currently lacks efficient methods for rapid quality testing. This research paper addressed this gap by introducing a portable device with employed near-infrared spectroscopy (NIR) to quickly assess the quality of peanut protein powder. The principal component analysis (PCA), partial least squares (PLS), and generalized regression neural network (GRNN) methods were used to construct the model to further enhance the accuracy and efficiency of the device. The results demonstrated that the newly established NIR method with PLS and GRNN analysis simultaneously predicted the fat, protein, and moisture of peanut protein powder. The GRNN model showed better predictive performance than the PLS model, the correlation coefficient in calibration (Rcal) of the fat, the protein, and the moisture of peanut protein powder were 0.995, 0.990, and 0.990, respectively, and the residual prediction deviation (RPD) were 10.82, 10.03, and 8.41, respectively. The findings unveiled that the portable NIR spectroscopic equipment combined with the GRNN method achieved rapid quantitative analysis of peanut protein powder. This advancement holds a significant application of this device for the industry, potentially revolutionizing quality testing procedures and ensuring the consistent delivery of high-quality products to fulfil consumer desires. Full article
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15 pages, 5807 KiB  
Article
Biodegradable Ca2+ Doped Mesoporous Silica Nanoparticles Promote Chemotherapy Synergism with Calcicoptosis and Activate Anti-Tumor Immunity
by Chao Liu, Xiaohui Tang and Gaofei Huang
Inorganics 2024, 12(6), 152; https://doi.org/10.3390/inorganics12060152 - 31 May 2024
Abstract
Mesoporous silica nanoparticles (MSNs), an excellent carrier material, have been widely used in tumor therapy as a vector for numerous therapeutic substances to boost therapeutical efficiency and specificity, such as loading them with chemotherapy drugs to improve the efficacy of chemotherapy. Nevertheless, they [...] Read more.
Mesoporous silica nanoparticles (MSNs), an excellent carrier material, have been widely used in tumor therapy as a vector for numerous therapeutic substances to boost therapeutical efficiency and specificity, such as loading them with chemotherapy drugs to improve the efficacy of chemotherapy. Nevertheless, they still face hurdles, such as lack of specificity and poor efficacy of monotherapy. The construction of multifunctional MSNs with excellent therapeutic effects by introducing metal ions has attracted the attention of many researchers. Herein, we demonstrated a calcium doped, chemotherapy drug doxorubicin (Dox) loaded, specific degradation nanoplatform, prepared using the sol–gel method by introducing calcium ions into an MSN framework, which enabled the doped nanoplatform to enhance chemotherapy and activate anti-tumor immune response. As a proof of concept, the doping of Ca2+ endowed MSNs with excellent specific degradation and pH responsive drug release, and enabled the synergy of chemotherapy and calcicoptosis. Furthermore, this nanoplatform also effectively elicited immunogenic cell death (ICD) and promoted the maturation of dendritic cells (DCs), realizing the activation of the anti-tumor immune system. The Ca2+ doped MSNs (CMSNs), that can activate immune response with specific degradation capability, demonstrate a practical strategy for the effective synergy between chemotherapy and calcicoptosis, providing a new paradigm for promoting chemotherapy-related treatment. Full article
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12 pages, 5316 KiB  
Article
Study on the Deactivation Mechanism of Ru/C Catalysts
by Zhi Cao, Tianchi Li, Baole Li, Xiwen Chen, Chen Zuo and Weifang Zheng
Processes 2024, 12(6), 1138; https://doi.org/10.3390/pr12061138 - 31 May 2024
Abstract
Employing catalytic decomposition to break down reducing agents in intermediate-level radioactive waste during nuclear fuel reprocessing offers significant advantages. This study focuses on investigating the deactivation behavior of 5% Ru/C catalysts by two different synthesis processes used for reducing agent destruction. Deactivation experiments [...] Read more.
Employing catalytic decomposition to break down reducing agents in intermediate-level radioactive waste during nuclear fuel reprocessing offers significant advantages. This study focuses on investigating the deactivation behavior of 5% Ru/C catalysts by two different synthesis processes used for reducing agent destruction. Deactivation experiments were conducted by subjecting the 5% Ru/C catalysts to 100 and 150 reaction cycles. Changes in the concentration of free radicals on the carbon-based carrier were measured to analyze the loading position and loss of Ru ions. Additionally, sorption–desorption curves and pore size distributions of the four catalysts were obtained. Analysis results reveal that Ru ions on the catalyst adsorb onto active free radical sites on the carbon-based carrier. Under ultrasonic conditions, some Ru ions partially desorb from the free radical sites on the carbon-based carrier, and desorbed Ru ions may adsorb onto weak free radical sites, while undesorbed Ru ions may adsorb onto strong free radical sites. After hundreds of hours of reaction, SM1 and SM2 exhibited approximately a 30% decrease in specific surface area and pore volume compared to SM0. However, the catalyst activity remained unchanged, and the catalyst pore size remained essentially unchanged, which primarily means that the micropores on the catalyst’s surface have undergone corrosion and damage. Full article
(This article belongs to the Section Catalysis Enhanced Processes)
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18 pages, 6496 KiB  
Article
Production Feature Analysis of Global Onshore Carbonate Oil Reservoirs Based on XGBoost Classier
by Guilin Qi and Baolei Liu
Processes 2024, 12(6), 1137; https://doi.org/10.3390/pr12061137 - 31 May 2024
Abstract
Carbonate reservoirs account for 60% of global reserves for oil, making them one of the most important types of sedimentary rock reservoirs for petroleum production. This study aimed to identify key production features that significantly impact oil production rates, enhancing reservoir management and [...] Read more.
Carbonate reservoirs account for 60% of global reserves for oil, making them one of the most important types of sedimentary rock reservoirs for petroleum production. This study aimed to identify key production features that significantly impact oil production rates, enhancing reservoir management and optimizing production strategies. A comprehensive dataset is built from reserves and production history data of 377 onshore carbonate oilfields globally, encompassing features such as production, recovery rate, and recovery degree of the whole lifecycle of an oilfield. XGBoost classifier is trained by K-fold cross-validation and its hyperparameters are optimized by Optuna optimization framework. The results show that XGBoost has the best performance evaluated with metrics including accuracy, precision, recall, and F1 score comparing with decision tree, random forest, and support vector machine. Key production features are identified by analyzing the classification feature importance of XGBoost classifier, including build-up stage cumulative production, plateau stage cumulative production, plateau stage recovery rate, plateau stage recovery degrees, and peak production. In conclusion, oilfield reserve size, build-up stage cumulative production, plateau stage cumulative production, and peak production increase, while plateau stage recovery rate decreases, and the plateau stage recovery degree of small-sized oilfields is slightly greater than that of moderate and large oilfields. The research methodology of this study can serve as a reference for studying production features of other types of oil and gas reservoirs. By applying the methodology to low-permeability oilfields, this paper concludes the key production features that are as follows: low-permeability oilfields generally have lower peak recovery rate, lower plateau stage recovery rate, lower decline stage recovery degree, and lower decline stage recovery rate, along with a wide but generally lower range of decline stage cumulative production compared to conventional oilfields. Full article
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14 pages, 3261 KiB  
Article
A Lightweight Safety Helmet Detection Algorithm Based on Receptive Field Enhancement
by Changpeng Ji, Zhibo Hou and Wei Dai
Processes 2024, 12(6), 1136; https://doi.org/10.3390/pr12061136 - 31 May 2024
Abstract
Wearing safety helmets is an important way to ensure the safety of workers’ lives. To address the challenges associated with low accuracy, large parameter values, and slow detection speed of existing safety helmet detection algorithms, we propose a receptive field-enhanced lightweight safety helmet [...] Read more.
Wearing safety helmets is an important way to ensure the safety of workers’ lives. To address the challenges associated with low accuracy, large parameter values, and slow detection speed of existing safety helmet detection algorithms, we propose a receptive field-enhanced lightweight safety helmet detection algorithm called YOLOv5s-CR. First, we use a lightweight backbone, a high-resolution feature fusion network, and a small object detection layer to improve the detection accuracy of small objects while substantially decreasing the model parameters. Next, we embed a coordinate attention mechanism into the feature extraction network to improve the localization accuracy of the detected object. Finally, we propose a new receptive field enhancement module (RFEM) to substitute the SPPF module in the original network, enabling the model to acquire features under multiple receptive fields, thereby enhancing the detection precision of multi-scale objects. Using the Safety Helmet Detection dataset for validation, in contrast to the initial YOLOv5s, the parameters of the improved algorithm were reduced by 62.8% to 2.61 M, and P, R, and mAP0.5 were increased by 1.5%, 1.2%, and 2.0%, respectively. The detection speed can reach 149FPS on the RTX3070 GPU, which satisfies the accuracy and real-time requirements for detecting safety helmets. Full article
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