Development of high-performance cutting tool materials is one of the critical parameters enhancing the surface finishing of high-speed machined products. Ti(C,N)-based cermets reinforced with and without different contents of silicon nitride were designed and evaluated to satisfy the requirements. In fact, the effect of silicon nitride addition to Ti(C,N)-based cermet remains unclear. The purpose of this study is to investigate the influence of Si₃N₄ additive on microstructure, mechanical properties, and thermal stability of Ti(C,N)-based cermet cutting tools. In the present work, $\alpha$-Si₃N₄ “grade SN-E10” was utilized with various fractions up to 6 wt.% in the designed cermets. A two-step reactive sintering process under vacuum was carried out for the green compact of Ti(C,N)-based cermet samples. The samples with 4 wt.% Si₃N₄ have an apparent solid density of about 6.75 g/cm³ (relative density of about 98 %); however, the cermet samples with 2 wt.% Si₃N₄ exhibit a superior fracture toughness of 10.82 MP${\mathrm{a.m}}^{1/2}$ and a traverse rupture strength of 1425.8 MPa. With an increase in the contents of Si₃N₄, the Vickers hardness and fracture toughness of Ti(C,N)-based cermets have an inverse behavior trend. The influence of Si₃N₄ addition on thermal stability is clarified to better understand the relationship between thermal stability and mechanical properties of Ti(C,N)-based cermets. Full article

Freeze–thaw effect is one of the most important environmental conditions that rocks may be subjected to. Through laboratory model tests, the damage characteristics of rocks under the FTC were studied. Based on assuming that the strength of rocks subject to the FTC follows the Weibull distribution, the cumulative damage variable of the number of FTCs was introduced. A cumulative damage constitutive model of shear strength attenuation of rock that meets the Mohr–Coulomb criterion is established. The rationality and applicability of the proposed damage constitutive model are verified by comparing the results of rock shear strength parameters under cyclic freeze–thaw loads. Full article

Batteries with an energy storage capacity of 280 Ah play a crucial role in promoting the development of smart grids. However, the inhomogeneity of their internal temperature cannot be accurately measured at different constant charge and discharge power, affecting the efficiency and safety of the battery. This work adopts finite element analysis to determine the typical internal temperature of a single-cell model, which can guide the measuring position of the battery. Before the manufacturing process, a slim pre-buried sensor is utilized to reduce the negative impacts of different constant charge and discharge powers. The maximum internal temperature of the battery is up to 77 °C at a constant charge and discharge power of 896 W. The temperature difference between the two poles and the battery surface is as high as 26.2 °C, which is beyond the safety temperature (55 °C). This phenomenon will result in the degradation of the positive electrode through dQ/dV curves. These measurements of battery internal temperature can improve battery heat control and facilitate the development of energy storage technology. Full article

The transformation and storage of energy and carbon dioxide in deep reservoirs include underground coal gasification, the underground storage of oil and gas, the underground storage of hydrogen, underground compressed air energy storage, the geological utilization and storage of carbon dioxide, etc [...] Full article

Sweet basil (Ocimum basilicum L.) is an important aromatic plant with high edibility and economic value, widely distributed in many regions of the tropics including the south of China. In recent years, environmental problems, especially soil salinization, have seriously restricted the planting and spread of sweet basil. However, the molecular mechanism of the salt stress response in sweet basil is still largely unknown. In this study, seed germination, seedling growth, and chlorophyll synthesis in sweet basil were inhibited under salt stress conditions. Through comparative transcriptome analysis, the gene modules involved in the metabolic processes, oxidative response, phytohormone signaling, cytoskeleton, and photosynthesis were screened out. In addition, the landscape of transcription factors during salt treatment in sweet basil was displayed as well. Moreover, the overexpression of the WRKY transcription factor-encoding gene, ObWRKY16, and the phenylalanine ammonia-lyase-encoding gene, ObPAL2, enhanced the seed germination, seedling growth, and survival rate, respectively, of transgenic Arabidopsis, suggesting that they might be important candidates for the creation of salt-tolerant sweet basil cultivars. Our data enrich the study on salt responses in sweet basil and provide essential gene resources for genetic improvements in sweet basil in the future. Full article

The mosquito Aedes aegypti is distributed worldwide and is recognized as the primary vector for dengue in numerous countries. To investigate whether the fitness cost of a single DENV-1 isolate varies among populations, we selected four Ae. aegypti populations from distinct localities: Australia (AUS), Brazil (BRA), Pakistan (PAK), and Peru (PER). Utilizing simple methodologies, we concurrently assessed survival rates and fecundity. Overall, DENV-1 infection led to a significant decrease in mosquito survival rates, with the exception of the PER population. Furthermore, infected Ae. aegypti from PAK, the population with the lowest infection rate among those tested, exhibited a noteworthy reduction in egg laying. These findings collectively suggest that local mosquito-virus adaptations may influence dengue transmission in endemic settings. Full article

Beer is a popular alcoholic beverage worldwide. However, limited research has been conducted on identifying key odor-active components in lager-type draft beers for the Chinese market. Therefore, this study aims to elucidate the odor characteristics of the four most popular draft beer brands through a sensory evaluation and an electronic nose. Subsequently, the four draft beers were analyzed through solid-phase microextraction and liquid–liquid extraction using a two-dimensional comprehensive gas chromatography–olfactometry–mass spectrometry analysis (GC×GC–O–MS). Fifty-five volatile odor compounds were detected through GC×GC–O–MS. Through an Aroma Extract Dilution Analysis, 22 key odor-active compounds with flavor dilution factors ≥ 16 were identified, with 11 compounds having odor activity values > one. An electronic nose analysis revealed significant disparities in the odor characteristics of the four samples, enabling their distinct identification. These findings help us to better understand the flavor characteristics of draft beer and the stylistic differences between different brands of products and provide a theoretical basis for objectively evaluating the quality differences between different brands of draft beer. Full article

While two Plasmodium falciparum circumsporozoite protein-based pre-erythrocytic vaccines (PEV), RTS,S and R21, have been approved by the WHO, no blood-stage vaccine (BSV) or transmission-blocking vaccine (TBV) has reached a phase 3 trial. One of the major obstacles that slows down malaria vaccine development is the shortage (or lack) of in vitro assays or animal models by which investigators can reasonably select the best vaccine formulation (e.g., antigen, adjuvant, or platform) and/or immunization strategy (e.g., interval of inoculation or route of immunization) before a human phase 2 trial. In the case of PEV, RTS,S and R21 have set a benchmark, and a new vaccine can be compared with (one of) the approved PEV directly in preclinical or early clinical studies. However, such an approach cannot be utilized for BSV or TBV development at this moment. The focus of this review is in vitro assays or in vivo models that can be used for P. falciparum BSV or TBV development, and I discuss important considerations during assay selection, standardization, qualification, validation, and interpretation of the assay results. Establishment of a robust assay/model with proper interpretation of the results is the one of key elements to accelerate future vaccine development. Full article

Supporting the decision-making process for the production of a 3D-printed hand exoskeleton within the Industry 4.0 and Industry 5.0 paradigms brings new concepts of manufacturing procedures for 3D-printed medical devices, including hand exoskeletons for clinical applications. The article focuses on current developments in the design and manufacturing of hand exoskeletons and their future directions from the point of view of implementation within the Industry 4.0 and Industry 5.0 paradigms and applications in practice. Despite numerous publications on the subject of hand exoskeletons, many have not yet entered production and clinical application. The results of research on hand exoskeletons to date indicate that they achieve good therapeutic effects not only in terms of motor control, but also in a broader context: ensuring independence and preventing secondary motor changes. This makes interdisciplinary research on hand exoskeletons a key study influencing the future lives of patients with hand function deficits and the further work of physiotherapists. The main aim of this article is to check in what direction hand exoskeletons can be developed from a modern economic perspective and how decision support systems can accelerate these processes based on a literature review, expert opinions, and a case study. Full article

Breast cancer is the most common cancer amongst women worldwide. Recently, owing to screening programs and new technologies, the survival rate has increased significantly. Breast cancer can potentially develop metastases, and, despite them, lung metastases generally occur within five years of breast cancer diagnosis. In this study, the objective was to analyze the effect of breast cancer-derived EVs on a lung epithelial cell line. BEAS-2B cells were treated with extracellular vesicles (EVs) derived from triple-negative breast cancer cells (TNBCs), e.g., MDA-MB-231 and HS578T, separated using differential ultracentrifugation. We observed an increased growth, migration, and invasiveness of normal epithelial lung cells over time in the presence of TNBC EVs compared to the control. Therefore, these data suggest that EVs released by tumor cells contain biological molecules capable of influencing the pro-tumorigenic activity of normal cells. Exploring the role of EVs in oncology research and their potential cargo may be novel biomarkers for early cancer detection and further diagnosis. Full article

Decentralization is a globally accepted concept of separation, the diversity of structures within the political system, the transfer of resources, activities, and powers in the decision-making process, and the division of tasks from the central government to lower authorities. It is implemented in both developed and developing countries, with different aims and consequences. In Serbia, the urban-centric development model and centralized governance caused rural settlements to become non-viable, and services and opportunities were deprived. This research has been undertaken in order to provide a deeper understanding of the territorial aspect of decentralization in Serbia and how it affects rural areas. It strives to offer a methodological framework for the identification of central settlements in rural areas of Serbia that will serve as a focal point for settlements networking in order to facilitate the spatial integration of rural areas and governance at the local level. This research encompasses (i) the identification of rural nodes as bearers of balanced governance and spatial development; (ii) the delimitation of their gravity sphere; and (iii) the determination of the relationship between settlement organization models and decentralization in Serbia. The research results indicate that identified rural nodes can be valuable to developing a holistic understanding of rural issues, supporting a bottom-up approach and regional disparities mitigation. Full article

Traditional methods for motion blur, often relying on a single layer, deviate from the correct colors. We propose a multilayer rendering method that closely approximates the motion blur effect. Our approach stores motion vectors for each pixel, divides these vectors into multiple sample points, and performs a backward search from the current pixel. The color at a sample point is sampled if it shares the same motion vector as its origin. This procedure repeats across layers, with only the nearest color values sampled for depth testing. The average color sampled at each point becomes that of the motion blur. Our experimental results indicate that our method significantly reduces the color deviation commonly found in traditional approaches, achieving structural similarity index measures (SSIM) of 0.8 and 0.92, which represent substantial improvements over the accumulation method. Full article

Dietary supplementation with n-3 polyunsaturated fatty acids (PUFA) has been found to be beneficial in rodent rheumatoid arthritis models and human trials. However, the molecular targets of n-3 PUFAs and their beneficial effects on rheumatoid arthritis are under-researched. Free fatty acid receptor 4 (FFA4, also known as GPR120) is a receptor for n-3 PUFA. We aim to investigate whether FFA4 activation reduces collagen-induced rheumatoid arthritis (CIA) by using an FFA4 agonist, compound A (CpdA), in combination with DBA-1J Ffa4 gene wild-type (WT) and Ffa4 gene knock-out (KO) mice. CIA induced an increase in the arthritis score, foot edema, synovial hyperplasia, pannus formation, proteoglycan loss, cartilage damage, and bone erosion, whereas the administration of CpdA significantly suppressed those increases in Ffa4 WT mice but not Ffa4 gene KO mice. CIA increased mRNA expression levels of pro-inflammatory Th1/Th17 cytokines, whereas CpdA significantly suppressed those increases in Ffa4 WT mice but not Ffa4 gene KO mice. CIA induced an imbalance between Th1/Th17 and Treg cells, whereas CpdA rebalanced them in spleens from Ffa4 WT mice but not Ffa4 gene KO mice. In SW982 synovial cells, CpdA reduced the LPS-induced increase in pro-inflammatory cytokine levels. In summary, the present results suggest that the activation of FFA4 in immune and synovial cells could suppress the characteristics of rheumatoid arthritis and be an adjuvant therapy. Full article

In daily life, counterfeit and substandard products, particularly currency, medicine, food, and confidential documents, are capable of bringing about very serious consequences. The development of anti-counterfeiting and authentication technologies with multilevel securities is a powerful means to overcome this challenge. Among various anti-counterfeiting technologies, fluorescent anti-counterfeiting technology is well-known and commonly used to fight counterfeiters due to its wide material source, low cost, simple usage, good concealment, and simple response mechanism. Spiropyran is favored by scientists in the fields of anti-counterfeiting and information encryption due to its reversible photochromic property. Here, we summarize the current available spiropyran-based fluorescent materials from design to anti-counterfeiting applications. This review will be help scientists to design and develop fluorescent anti-counterfeiting materials with high security, high performance, quick response, and high anti-counterfeiting level. Full article

Non-metallic armoured optoelectronic cable winch systems (NAOCWSs) play critical roles in facilitating signal transmission and powering subsea equipment. Due to the varying depths in these applications, deploying the entire cable length is unnecessary. However, the portion of the cable that remains coiled around the winch can generate an electromagnetic field, which may interfere with signal transmission and induce electromagnetic heating. This can lead to elevated temperatures within the system, affecting the cable’s lifespan. Consequently, this study examines the distributions of magnetic and temperature fields within the NAOCWS with different currents (10–30 A) and numbers of winding layers (1–10). Findings indicate that the magnetic flux density (MFD) changes periodically, and the period is closely related to the distance between the cables. At the centre of the cable, the flux density is minimum. Temperature distribution correlates with both current amplitude and the number of winding layers, where an increase in either parameter amplifies the temperature variance between the edge and intermediate cables within the same layer. The current does not affect the internal temperature distribution pattern. With the number of winding layers determined, the layer where the highest temperature of the system is located is well defined and does not vary with current. Full article

Since 2018, the authors have been working on reconstructing the exterior of the Great Pitiunt Roman fortress in 3D. This article presents the results of the visualization of the exterior and interior of the temple complex. During the study, the dimensions and plans of the site were analyzed, revealing discrepancies in various sources. To clarify the complex’s dimensions, aerial photography using UAV was conducted, and photogrammetric models, orthophoto, and digital surface models were created. The research also uncovered previously unrecorded architectural features. During the reconstruction of the temples, much attention was paid to the structural design of the buildings, connections and load distribution. Engineering calculations have been carried out for the clarification of the structural solutions. The article presents the results of a detailed reconstruction of the exterior, interior and structural features of Temple Nos. 1–4, based on preserved archaeological evidence, excavation results, contemporaneous Early Christian sites, and an analysis of the materials and technologies used at the time. The reconstruction of the mosaic floor of Temple No. 2 allowed a realistic visualization of the interior. Full article

Can there be an ethics of politics or political ethics in authoritarian populist democracies? Given that politics operates under potentially risky and morally ambiguous methods that involve strategic calculations with some elements of irrationality, developing an ethics of political leadership can be an abstruse exercise. The frequent mismatch between political methods, objectives, and outcomes exemplifies the complexity of political reality and its unpredictability. The existential tension between utilitarian and deontological approaches to ethics frequently arises in politics. However, to address the failures of democracy in political leadership, it is imperative to explore the ethical implications of authoritarian populism. By scrutinizing how authoritarian populism undermines democratic principles and polarizes societies, one can better understand its moral challenges and work towards developing a more ethical framework for political leadership. This article discusses Catholic political ethics in response to populist authoritarianism from three different perspectives: first, it analyzes what populist authoritarianism entails and its limitations in political governance; second, it discusses diverse components of political ethics, highlighting realistic dilemmas in the application of each component; and third, it makes an ethical evaluation of populist authoritarianism from the Catholic social ethics perspective, emphasizing human dignity, common good, and solidarity as key to countering populist authoritarianism. The article makes two ethical suggestions in line with Catholic political ethics to address populist authoritarianism. The first is the principle of social cohesion, aimed at addressing the social, ethnic, racial, political, economic, and religious divisions that characterize populist authoritarian leadership. The second is the principle of social recognition, used as a strategy for safeguarding the common good by centralizing human dignity. Full article

The identification of anticancer therapies using next-generation sequencing (NGS) is necessary for the treatment of cholangiocarcinoma. NGS can be easily performed when cell blocks (CB) are obtained from bile stored overnight. We compared NGS results of paired CB and surgically resected specimens (SRS) from the same cholangiocarcinoma cases. Of the prospectively collected 64 bile CBs from 2018 to 2023, NGS was performed for three cases of cholangiocarcinoma that could be compared with the SRS results. The median numbers of DNA and RNA reads were 95,077,806 [CB] vs. 93,161,788 [SRS] and 22,101,328 [CB] vs. 24,806,180 [SRS], respectively. We evaluated 588 genes and found that almost all genetic alterations were attributed to single-nucleotide variants, insertions/deletions, and multi-nucleotide variants. The coverage rate of variants in SRS by those found in CB was 97.9–99.2%, and the coverage rate of SRS genes by CB genes was 99.6–99.7%. The NGS results of CB fully covered the variants and genetic alterations observed in paired SRS samples. As bile CB is easy to prepare in general hospitals, our results suggest the potential use of bile CB as a novel method for NGS-based evaluation of cholangiocarcinoma. Full article

Recent advancements in quadrupedal robotics have explored the motor potential of these machines beyond simple walking, enabling highly dynamic skills such as jumping, backflips, and even bipedal locomotion. While reinforcement learning has demonstrated excellent performance in this domain, it often relies on complex reward function tuning and prolonged training times, and the interpretability is not satisfactory. Riemannian motion policies, a reactive control method, excel in handling highly dynamic systems but are generally limited to fully actuated systems, making their application to underactuated quadrupedal robots challenging. To address these limitations, we propose a novel framework that treats each leg of a quadrupedal robot as an intelligent agent and employs multi-agent reinforcement learning to coordinate the motion of all four legs. This decomposition satisfies the conditions for utilizing Riemannian motion policies and eliminates the need for complex reward functions, simplifying the learning process for high-level motion modalities. Our simulation experiments demonstrate that the proposed method enables quadrupedal robots to learn stable locomotion using three, two, or even a single leg, offering advantages in training speed, success rate, and stability compared to traditional approaches, and better interpretability. This research explores the possibility of developing more efficient and adaptable control policies for quadrupedal robots. Full article

Extracellular vesicles (EVs), including exosomes, microvesicles, and other lipid vesicles derived from cells, play a pivotal role in intercellular communication by transferring information between cells. EVs secreted by progenitor and stem cells have been associated with the therapeutic effects observed in cell-based therapies, and they also contribute to tissue regeneration following injury, such as in orthopaedic surgery cases. This review explores the involvement of EVs in nerve regeneration, their potential as drug carriers, and their significance in stem cell research and cell-free therapies. It underscores the importance of bioengineers comprehending and manipulating EV activity to optimize the efficacy of tissue engineering and regenerative therapies. Full article

Ultra-wide-field optical coherence tomography (UWF-OCT) has been recently introduced into clinical ophthalmological practice. To date, there are few data on the reference values of the retinal thickness (RT) and choroidal thickness (CT) measured with this technique. This study aimed to analyze the variance in RT and CT in the healthy eyes of white Caucasian patients with UWF-OCT tests performed with the largest available scan size of 23 × 20 mm. The data were analyzed with reference to the patients’ age and gender and the axial length of the eyeball. The results of UWF-OCT scanning enabled us to visualize the shape of the retina and choroid in a large portion of the eyeball. Both anatomical entities became significantly thinner at the periphery. The peripheral CT was greater in the upper and temporal sectors; the RT was higher in the nasal compared to the temporal sectors. Both the choroid and retina showed a reduced thickness with age; however, the CT and RT did not show a statistically significant correlation with the axial length after adjusting for age and gender. Age-related variations in thickness were especially prominent in the choroid. The CT in UWF-OCT testing was significantly greater in females, while the RT was greater in males. UWF-OCT testing provides additional information on the anatomical structure of the retina and choroid compared to standard-field OCT. Full article

Background: Fluorescence visualization of pathologies, primarily neoplasms in human internal cavities, is one of the most popular forms of diagnostics during endoscopic examination in medical practice. Currently, visualization can be performed in the augmented reality mode, which allows to observe areas of increased fluorescence directly on top of a usual color image. Another no less informative form of endoscopic visualization in the future can be mapping (creating a mosaic) of the acquired image sequence into a single map covering the area under study. The originality of the present contribution lies in the development of a new 3D bimodal experimental bladder model and its validation as an appropriate phantom for testing the combination of bimodal cystoscopy and image mosaicking. Methods: An original 3D real bladder-based phantom (physical model) including cancer-like fluorescent foci was developed and used to validate the combination of (i) a simultaneous white light and fluorescence cystoscopy imager with augmented reality mode and (ii) an image mosaicking algorithm superimposing both information. Results: Simultaneous registration and real-time visualization of a color image as a reference and a black-and-white fluorescence image with an overlay of the two images was made possible. The panoramic image build allowed to precisely visualize the relative location of the five fluorescent foci along the trajectory of the endoscope tip. Conclusions: The method has broad prospects and opportunities for further developments in bimodal endoscopy instrumentation and automatic image mosaicking. Full article