The social restriction measures implemented due to the COVID-19 pandemic have impacted the pattern of occurrences of respiratory viruses. According to surveillance results in the Gwangju region of South Korea, respiratory syncytial virus (RSV) did not occur during the 2020/2021 season. However, there was a delayed resurgence in the 2021/2022 season, peaking until January 2022. To analyze this, a total of 474 RSV positive samples were investigated before and after the COVID-19 pandemic. Among them, 73 samples were selected for whole-genome sequencing. The incidence rate of RSV in the 2021/2022 season after COVID-19 was found to be approximately three-fold higher compared to before the pandemic, with a significant increase observed in the age group from under 2 years old to under 5 years old. Phylogenetic analysis revealed that, for RSV-A, whereas four lineages were observed before COVID-19, only the A.D.3.1 lineage was observed during the 2021/2022 season post-pandemic. Additionally, during the 2022/2023 season, the A.D.1, A.D.3, and A.D.3.1 lineages co-circulated. For RSV-B, while the B.D.4.1.1 lineage existed before COVID-19, both the B.D.4.1.1 and B.D.E.1 lineages circulated after the pandemic. Although atypical RSV occurrences were not due to new lineages, there was an increase in the frequency of mutations in the F protein of RSV after COVID-19. These findings highlight the need to continue monitoring changes in RSV occurrence patterns in the aftermath of the COVID-19 pandemic to develop and manage strategies in response. Full article

This study explores the pedagogical challenges in teaching chemical equations, focusing on the ability to balance chemical equations and understanding related basic chemical concepts among chemistry teacher students. Based on Johnstone’s representation theory, we examined the efficacy of digital tools designed to enhance equation-balancing skills. The key findings of this study seem to be the disconnection between students’ procedural proficiency in balancing equations and their conceptual understanding of underlying chemical reactions. Surprising was the relatively low ability to balance chemical equations when compared to the expected level of upper-secondary school students. An analysis of the results showed that students could be distinguished into four different groups of solvers based on their total score and solving time: efficient, persistent, impulsive, and inefficient solvers. Utilizing an eye-tracking study, the findings reveal a predominant reliance on symbolic representations, with additional sub-microscopic representations provided by digital applets failing to significantly improve equation-balancing capabilities. This reliance potentially hinders students’ ability to conceptualize reactions at the particulate level, impacting overall chemistry comprehension. This study underscores the need for a pedagogical shift toward integrating multiple representations into chemical education to bridge the gap between procedural skills and deep conceptual understanding. By highlighting the limitations of current teaching approaches and the use of digital tools, this research contributes to improving chemical education strategies, fostering a more integrated and nuanced understanding of chemical principles among students. Full article

(1) Background: We aimed to identify the possible relationship between various diseases of the upper digestive system and colon polyps by analyzing patients with gastric polyps and evaluating the cancers and diseases accompanying the polyps. (2) Methods: Each patient’s age; gender; polyp type and size; presence of Helicobacter pylori (H. pylori), atrophic gastritis, and intestinal metaplasia; status of whether cancer developed during follow-up; status of whether a colonoscopy was performed or not; and colon pathologies detected during colonoscopy were analyzed retrospectively using hospital records. (3) Results: Between the study dates, 19,214 esophagogastroduodenoscopies were performed in the endoscopy unit of our hospital. Gastric polyps were detected in 178 (0.9%) patients. No significant relationship was found between the gastric polyp size and the occurrence of gastric cancer or gastrointestinal system malignancy (p > 0.05). A colonoscopy was performed in 86 of the 178 patients who underwent gastroscopy. The frequency of polyp detection during colonoscopy was statistically significantly higher in patients with gastric polyps than in patients without gastric polyps (p < 0.001). (4) Conclusions: New prospective studies are needed regarding the relationship between gastric polyps and gastrointestinal system diseases. Going forward, a colonoscopy will be required in gastric polyp patients, especially with FGP. Full article

Dynamic environmental conditions, such as climate change and host availability, have greatly influenced the expansion of medically relevant tick vectors into new regions throughout the southeastern United States of America. As tick populations migrate into new areas, it has been suggested they can exhibit a phenomenon known as incomplete feeding. With this phenomenon, tick vectors feed on more than one host at each life stage, thus increasing the likelihood of pathogen transmission. Although this behavior is not well understood, it presents an important threat to human health. Here we present evidence of incomplete feeding behaviors in multiple tick species in South Carolina. Engorged, blood-fed female ticks were collected from feral dogs at animal shelters across South Carolina in 2022. All ticks were tested for human blood meals using rapid stain identification blood tests. Approximately one third (33.78%) of all ticks tested positive for a human blood meal, with various patterns seen across species, geographic location, and collection month. The results of this pilot study follow the current national trend of increasing rates of tick-borne disease incidence in the southeastern United States of America and warrant further investigation into the relationship between seasonality, geographic distribution, species, and incomplete feeding among tick populations in South Carolina. Full article

Abstract: Rubberized concrete (RC) has received widespread attention due to its energy absorption and crack resistance properties. However, due to its low compressive strength, it is not recommended for structural applications. The rubber size and content affect RC’s mechanical properties. This study investigated and formulated the behavior of RC with different particle sizes and contents under dynamic and static loading. Quasi-static compressive and dynamic tests were conducted on RC with varying content of rubber (0–30%) and rubber sizes (0.1–20 mm). It was found that the rubber particle size was 0.5mm and the rubber content was 2%. An equation was derived from the experimental data to forecast the impact of rubber size and content on compressive strength. Additionally, by combining the literature and this research’s data, a model was established based on neural networks to predict the strength of RC. SHPB tests were carried out to study the stress–strain curves under dynamic load. The peak stress, fragment analysis, and energy absorption of RC with varying content of rubber and rubber sizes at three different strain rates (100 s⁻¹, 160 s⁻¹, and 290 s⁻¹) were investigated. Equations describing the relationship between dynamic increase factor (DIF), rubber material content, and strain rate on different particle sizes were obtained by fitting. The DIF increased as the content of the rubber increased. By analyzing energy absorption data, it was found that the optimal ratio for energy absorption was RC-0.5-30, RC-0.1-30, and RC-10-30 at strain rates of 100 s⁻¹, 160 s⁻¹, and 290 s⁻¹. This study could be a good guideline for other researchers to easily select the content and size of the rubber in RC for their applications. It also has a positive significance in promoting the development of green building materials. Full article

Abstract: Within a lean context, the aim is to eliminate all forms of waste, including environmental waste, to improve productivity and reduce costs. Key to achieving this objective are operational performance and sustainability indicators. Lean companies must prioritize both operational performance and sustainability, remaining cognizant of their current status. With this in mind, the authors sought to ascertain whether lean companies demonstrate enhanced sustainability. Thus, the authors raised the following research question: does a lean company exhibit greater sustainability? However, these indicators have traditionally been measured independently, and few studies have indicated the need for a global indicator that could simultaneously address both. Such a global indicator would enable a clearer assessment and understanding of the trade-offs between operational performance and sustainability. This paper introduces such an integrated indicator, aiming to measure companies’ lean–green compliance by intertwining sustainability issues with overall equipment effectiveness (OEE). The authors have termed this indicator business overall performance and sustainability effectiveness (BOPSE). Its primary goal is to evaluate business effectiveness by considering both operational performance and sustainability compliance. The sustainability strand was drawn from, adapted, and simplified based on the Global Reporting Initiative (GRI). This development was framed in a lean–green environment, emphasizing continuous efforts to identify and reduce all sources of lean waste, alongside the waste prevention perspectives of cleaner production, environmental compliance, and social responsibility, which play crucial roles in shaping the factories of the future. This paper presents the background and development of the BOPSE model. To answer the research question, two research methods were undertaken: a survey and case studies. The model was applied in three distinct case studies, demonstrating its usefulness in discerning varying levels of lean–green compliance through this integrated indicator. Full article

Monitoring heart conditions through electrocardiography (ECG) has been the cornerstone of identifying cardiac irregularities. Cardiologists often rely on a detailed analysis of ECG recordings to pinpoint deviations that are indicative of heart anomalies. This traditional method, while effective, demands significant expertise and is susceptible to inaccuracies due to its manual nature. In the realm of computational analysis, Artificial Neural Networks (ANNs) have gained prominence across various domains, which can be attributed to their superior analytical capabilities. Conversely, Spiking Neural Networks (SNNs), which mimic the neural activity of the brain more closely through impulse-based processing, have not seen widespread adoption. The challenge lies primarily in the complexity of their training methodologies. Despite this, SNNs offer a promising avenue for energy-efficient computational models capable of displaying a high-level performance. This paper introduces an innovative approach employing SNNs augmented with an attention mechanism to enhance feature recognition in ECG signals. By leveraging the inherent efficiency of SNNs, coupled with the precision of attention modules, this model aims to refine the analysis of cardiac signals. The novel aspect of our methodology involves adapting the learned parameters from ANNs to SNNs using leaky integrate-and-fire (LIF) neurons. This transfer learning strategy not only capitalizes on the strengths of both neural network models but also addresses the training challenges associated with SNNs. The proposed method is evaluated through extensive experiments on two publicly available benchmark ECG datasets. The results show that our model achieves an overall accuracy of 93.8% on the MIT-BIH Arrhythmia dataset and 85.8% on the 2017 PhysioNet Challenge dataset. This advancement underscores the potential of SNNs in the field of medical diagnostics, offering a path towards more accurate, efficient, and less resource-intensive analyses of heart diseases. Full article

Recycled manure solids (RMSs) are widely utilised as beddings due to their economic and environmentally friendly features. Internal change in RMSs plays a vital role in the stable operation and management of beddings. However, the internal microenvironment of various manure beddings has not been fully reported. Therefore, we evaluated the physicochemical properties, internal gases and changes in the microbial community of the in situ fermentation beds, which were prefermented by cow manure with sawdust (FSD), straw (FST) and sawdust–straw mixture (FM), at a farm in Jiangsu, China, from June to September 2022. The results indicated that the FSD and FM beds were more capable of degrading organic matter (OM), accumulating total nitrogen and processing a more stable pH environment. FSD bed promoted the conversion of nitrate–nitrogen and ammonium–nitrogen (NH₄⁺-N). Different treatments and times had significant effects on bacterial and fungal communities. FSD enriched Chloroflexi, and FST enriched Actinobacteriota in the early stage, while FM enriched Proteobacteria in the late stage. Bacterial communities were more sensitive to NH₄⁺-N and OM, while fungal communities were more sensitive to temperature and pH. FSD had potential advantages concerning N conversion and C emission reduction. The results of the study revealed the microenvironmental dynamics during bedding use, providing a theoretical basis for the use of a compost bedding system for managing recycled dairy manure. Full article

A chitosan-based Cu²⁺ fluorescent probe was designed and synthesized independently using the C-2-amino group of chitosan with 1, 8-naphthalimide derivatives. A series of experiments were conducted to characterize the optical properties of the grafted probe. The fluorescence quenching effect was investigated based on the interactions between the probe and common metals. It was found that the proposed probe displayed selective interaction with Cu²⁺ over other metal ions and anions, reaching equilibrium within 5 min. Full article

In this paper, we propose a novel, vision-transformer-based end-to-end pose estimation method, LidPose, for real-time human skeleton estimation in non-repetitive circular scanning (NRCS) lidar point clouds. Building on the ViTPose architecture, we introduce novel adaptations to address the unique properties of NRCS lidars, namely, the sparsity and unusual rosetta-like scanning pattern. The proposed method addresses a common issue of NRCS lidar-based perception, namely, the sparsity of the measurement, which needs balancing between the spatial and temporal resolution of the recorded data for efficient analysis of various phenomena. LidPose utilizes foreground and background segmentation techniques for the NRCS lidar sensor to select a region of interest (RoI), making LidPose a complete end-to-end approach to moving pedestrian detection and skeleton fitting from raw NRCS lidar measurement sequences captured by a static sensor for surveillance scenarios. To evaluate the method, we have created a novel, real-world, multi-modal dataset, containing camera images and lidar point clouds from a Livox Avia sensor, with annotated 2D and 3D human skeleton ground truth. Full article

Protein hydrolysates (PH) and humic substances (HS) are biostimulants that promote crop growth. Despite the widening of knowledge and insights provided by research activities over the past thirty years, there are still uncertainties concerning the possible direct absorption by plants of organic nitrogen as amino acids (AA) delivered as PH and the role of HS. This study aimed to assess the impact of PH treatment on the amino acid profile of maize seedlings and the influence of HS on amino acid uptake. Maize seedlings were treated with either PH from the yeast of the sugarcane fermentation process or a synthetic mixture of amino acids mimicking the PH composition using dual-labeled amino acids (¹³C, ¹⁵N glutamic acid) combined with commercially available humic substances. The amino acid profile was analyzed using HPLC, and plant tissues were examined for ¹⁵N using nuclear magnetic resonance (NMR) and mass spectrometry. The application of PH stimulated maize growth, with a more significant effect observed in the presence of humic substances. The treatments significantly altered the plants’ total amino acid content and composition profile. Maize seedlings actively uptake amino acids, representing 21% of the total nitrogen composition. The ascertained improvement of amino acid uptake stimulated by humic substances is associated with their effect on the differential expression of amino permease transporters and plasma membrane H⁺-ATPase. PH and amino acids enhance maize growth by significantly increasing amino acid uptake. The addition of humic substances further improved the biostimulant effects. Full article

Binge alcohol consumption during adolescence can produce lasting deficits in learning and memory while also increasing the susceptibility to substance use disorders. The adolescent intermittent ethanol (AIE) rodent model mimics human adolescent binge drinking and has identified the nucleus basalis magnocellularis (NbM) as a key site of pathology. The NbM is a critical regulator of prefrontal cortical (PFC) cholinergic function and attention. The cholinergic phenotype is controlled pro/mature neurotrophin receptor activation. We sought to determine if p75NTR activity contributes to the loss of cholinergic phenotype in AIE by using a p75NTR modulator (LM11A-31) to inhibit prodegenerative signaling during ethanol exposure. Male and female rats underwent 5 g/kg ethanol (AIE) or water (CON) exposure following 2-day-on 2-day-off cycles from postnatal day 25–57. A subset of these groups also received a protective dose of LM11A-31 (50 mg/kg) during adolescence. Rats were trained on a sustained attention task (SAT) and behaviorally relevant acetylcholine (ACh) activity was recorded in the PFC with a fluorescent indicator (AChGRAB 3.0). AIE produced learning deficits on the SAT, which were spared with LM11A-31. In addition, PFC ACh activity was blunted by AIE, which LM11A-31 corrected. Investigation of NbM ChAT+ and TrkA+ neuronal expression found that AIE led to a reduction of ChAT+TrkA+ neurons, which again LM11A-31 protected. Taken together, these findings demonstrate the p75NTR activity during AIE treatment is a key regulator of cholinergic degeneration. Full article

Drought may considerably decrease the growth and yield of potatoes. Small tubers may have lower performance and be more sensitive to abiotic stresses than larger tubers. Since an increase in drought areas may be expected, the development of potato varieties with drought tolerance has become necessary. Two-year greenhouse experiments were conducted to test the drought tolerance of potato breeding lines (C103, C107, C20) with great osmotic stress tolerance. Minitubers with diameters of 25–35, 20–24, 15–19 and 10–14 mm were planted. Treatments were the optimal irrigated control (100%) and moderate and severe drought (60% and 20% of optimum water supply). To study the after-effects of drought, tubers from different treatments were planted separately the following year because seed tuber priming may increase drought tolerance. Seed tubers (25–35 mm), two irrigation treatments (control and severe drought), and two control cultivars were used in the second year. We observed the rate of emergence from day-after-planting (DAP) 20 to 30 and flowering from 48 to 54. NDVI measurements were performed on the DAP35-45-75. Plant height and fresh weight of aboveground biomass (AGB) were recorded on DAP76. Harvested tubers were counted, weighed, and size-categorized, and then the number and fresh tuber yield per plant (TN and TY) were calculated. Stress indices (SI) were calculated as percentages of the results of control plots to compare the responses of genotypes to drought stress. We found that each breeding line showed adequate drought tolerance, although only the C103 and C107 breeding lines were stable in in vivo conditions. SI values for tuber number/tuber yield were 103/57; 102/63; 83/52; 80/58 and 55/41 in C103, C107, C20, ‘Boglárka’ and ‘Desiree’ (the last two were control varieties), respectively. The size of the seed tuber significantly affected each character, and usually minitubers larger than 20 mm performed better than smaller ones. No significant after-effect of drought stress on the next generation was found. Although we found a positive correlation (r=0.83) between NDVI values and yield parameters, the correlations in our study were not consistent in all genotypes and water treatments. Full article

This study examined 430 Chinese college students’ engagement in arts activities and the psychological benefits derived from such activities. The research differentiated between various types of arts participation and ways of involvement and examined four potential positive psychological outcomes. The findings revealed correlations between (1) creative participation in the performing arts, ‘flow’, and aesthetic emotions; (2) consumptive participation in the visual arts and aesthetic emotions; and (3) creative participation in the literary arts and ego identity. Holistic arts participation demonstrated a significantly positive relationship with flourishing. A path analysis showed that flow experience and aesthetic emotions served as mediators in the mechanism through which holistic arts participation affected flourishing, with a chained mediation effect from flow experience to ego identity. This study confirms that arts participation is an effective pathway for individual flourishing and that more diverse and profound engagement in the arts can lead to sustained and widespread happiness. Full article

In order to cleanly and efficiently extract zirconium from zircon sand (the main component is ZrSiO₄), sodium hydroxide sub-molten salt was used to decompose ZrSiO₄ in this study. When ZrSiO₄ reacts with sodium hydroxide sub-molten salt, the formation of Na₂ZrSiO₅ (a water-insoluble product) considerably affects the separation efficiency of Zr and Si and increases production cost. Thus, it is necessary to control the formation of Na₂ZrSiO₅. The influence of NaOH content, reaction temperature, reaction time, and NaOH/ore mass ratio on the formation of Na₂ZrSiO₅ were systematically investigated. The optimum reaction parameters for the inhibition of Na₂ZrSiO₅ formation were as follows: 80% NaOH content, 245 °C reaction temperature, 4:1 NaOH/ore mass ratio, 10 h reaction time, and 400 r/min agitation speed. These results indicate that ZrSiO₄ is decomposed to Na₂ZrO₃ and Na₂SiO₃ by reacting with NaOH, realizing the separation of Zr and Si, and then the reactions between Na₂ZrO₃ and Na₂SiO₃ result in the formation of Na₂ZrSiO₅, during the decomposition of ZrSiO₄ using NaOH sub-molten salt. The sub-molten salt decomposition process can realize the clean extraction of zirconium, which is conducive to the sustainable development of zirconium resources. Full article

Hydrogen combustion engine vehicles have the potential to rapidly enter the market and reduce greenhouse gas emissions (GHG) compared to conventional engines. The ability to provide a rapid market deployment is linked to the fact that the industry would take advantage of the existing internal combustion engine production chain. The aim of this paper is twofold. First, it aims to develop a methodology for applying life-cycle assessment (LCA) to internal combustion engines to estimate their life-cycle GHG emissions. Also, it aims to investigate the decarbonization potential of hydrogen engines produced by exploiting existing diesel engine technology and assuming diverse hydrogen production routes. The boundary of the LCA is cradle-to-grave, and the assessment is entirely based on primary data. The products under study are two monofuel engines: a hydrogen engine and a diesel engine. The hydrogen engine has been redesigned using the diesel engine as a base. The engines being studied are versatile and can be used for a wide range of uses such as automotive, cogeneration, maritime, off-road, and railway; however, this study focuses on their application in pickup trucks. As part of the redesign process, certain subsystems (e.g., combustion, injection, ignition, exhaust gas recirculation, and exhaust gas aftertreatment) have been modified to make the engine run on hydrogen. Results revealed that employing a hydrogen engine using green hydrogen (i.e., generated from water electrolysis using wind-based electricity) might reduce GHG emission by over 90% compared to the diesel engine This study showed that the benefits of the new hydrogen engine solution outweigh the increase of emissions related to the redesign process, making it a potentially beneficial solution also for reconditioning current and used internal combustion engines. Full article

Carbon-fiber-reinforced polyimide (PI) resin composites have gained significant attention in the field of continuous-fiber-reinforced polymers, in which the interfacial bonding between carbon fiber and matrix resin has been an important research direction. This study designed and prepared a water-soluble thermoplastic polyamide acid sizing agent to improve the wettability of carbon fiber, enhance the van der Waals forces between carbon fiber and resin and strengthen the chemical bonding between the sizing agent and the alkyne-capped polyimide resin by introducing alkyne-containing functional groups into the sizing agent. This study found that the addition of a sizing layer effectively bridged the large modulus difference between the fiber and resin regions, resulting in the formation of an interfacial layer approximately 85 nm thick. This layer facilitated the transfer of stress from the matrix to the reinforced carbon fiber, leading to a significant improvement in the interfacial properties of the composites. Adjusting the concentration of the sizing agent showed that composites treated with 3% had the best interfacial properties. The interfacial shear strength increased from 82.08 MPa to 108.62 MPa (32.33%) compared to unsized carbon fiber. This research is significant for developing sizing agents suitable for carbon-fiber-reinforced polyimide composites. Full article

Bacteria, fungi, and protists occupy a pivotal position in maintaining soil ecology. Despite limited knowledge on their responses to managed vegetation restoration strategies in karst regions, we aimed to study the essential microbial communities involved in the process of vegetation restoration. We compared microbial characteristics in four land use types: planted forests (PF), forage grass (FG), a mixture of plantation forest and forage grass (FF), and cropland (CR) as a reference. Our findings revealed that the richness of bacteria and protists was higher in FF compared to PF, while fungal richness was lower in both PF and FF than in CR. Additionally, the bacterial Shannon index in FF was higher than that in CR and PF, while the fungal and protist Shannon indices were similar across all four land use types. Significant differences were observed in the compositions of bacterial, fungal, and protist communities between FF and the other three land use types, whereas bacterial, fungal, and protist communities were relatively similar in PF and FG. In FF, the relative abundance of bacterial taxa Acidobacteria, Firmicutes, and Gemmatimonadetes was significantly higher than in PF and CR. Fungal communities were dominated by Ascomycota and Basidiomycota, with the relative abundance of Ascomycota significantly higher in FF compared to other land use types. Regarding protistan taxa, the relative abundance of Chlorophyta was higher in FF compared to CR, PF, and FG, while the relative abundance of Apicomplexa was higher in CR compared to FF. Importantly, ammonium nitrogen, total phosphorus, and microbial biomass nitrogen were identified as key soil properties predicting changes in the diversity of bacteria, fungi, and protists. Our results suggest that the microbial community under FF exhibits greater sensitivity to vegetation restoration compared to PF and FG. This sensitivity may stem from differences in soil properties, the formation of biological crusts and root systems, and management activities, resulting in variations in bacterial, fungal, and protist diversity and taxa in PF. As a result, employing a combination restoration strategy involving plantation forest and forage grass proves to be an effective approach to enhance the microbial community and thereby improve ecosystem functionality in ecologically fragile areas. Full article

Type 2 diabetes mellitus (T2DM) management and glycemic control in underserved non-Hispanic Black adults presents with multifaceted challenges: balancing the optimal complexity of antihyperglycemic medications prescribed, limited medication access due to socioeconomic status, medication nonadherence, and high prevalence of cardiometabolic comorbidities. This single-center, cross-sectional, retrospective chart analysis evaluated the association of Medication Regimen Complexity (MRC) with cardiometabolic outcomes (glycemic, atherogenic cholesterol, and blood pressure control) among non-Hispanic Black adults with type 2 diabetes. Utilizing 470 independent patient electronic health records, MRC and other covariates were examined to determine their associations with cardiometabolic outcomes. Chi-square tests of independence and multiple logistic regression were performed to identify associations between MRC and cardiometabolic outcomes. Our findings indicate significant negative and positive associations between MRC and glycemic control and atherogenic cholesterol control, respectively. However, there were no associations between MRC and blood pressure control. As diabetes MRC was shown to be associated with poor glycemic control and improved atherogenic cholesterol control, there is a critical need to standardize interdisciplinary diabetes care to include pharmacists and to develop more insurance policy interventions that increase access to newer, efficacious diabetes medications for historically marginalized populations. Full article

The increase in Cervical Spondylosis cases and the expansion of the affected demographic to younger patients have escalated the demand for X-ray screening. Challenges include variability in imaging technology, differences in equipment specifications, and the diverse experience levels of clinicians, which collectively hinder diagnostic accuracy. In response, a deep learning approach utilizing a ResNet-34 convolutional neural network has been developed. This model, trained on a comprehensive dataset of 1235 cervical spine X-ray images representing a wide range of projection angles, aims to mitigate these issues by providing a robust tool for diagnosis. Validation of the model was performed on an independent set of 136 X-ray images, also varied in projection angles, to ensure its efficacy across diverse clinical scenarios. The model achieved a classification accuracy of 89.7%, significantly outperforming the traditional manual diagnostic approach, which has an accuracy of 68.3%. This advancement demonstrates the viability of deep learning models to not only complement but enhance the diagnostic capabilities of clinicians in identifying Cervical Spondylosis, offering a promising avenue for improving diagnostic accuracy and efficiency in clinical settings. Full article

Class IIa bacteriocins produced in lactic acid bacteria are short cationic peptides with antimicrobial activity. In the search for new biopreservation agents, class IIa bacteriocins are considered to be the best potential candidates, not only due to their large abundance but also because of their high biological activity and excellent thermal stability. However, regulated by the biosynthetic regulatory system, the natural class IIa bacteriocin yield is low, and the extraction process is complicated. The biotechnological production of class IIa bacteriocins in various cell factories has been attempted to improve this situation. In this review, we focus on the application of biotechnological routes for class IIa bacteriocin production. The drawbacks and improvements in the production of class IIa bacteriocins in various cell factories are discussed. Furthermore, we present the main challenge of class IIa bacteriocins, focusing on increasing their production by constructing suitable cell factories. Recombinant bacteriocins have made considerable progress from inclusion body formation, dissolved form and low antibacterial activity to yield recovery. The development of prospective cell factories for the biotechnological production of bacteriocins is still required, which may facilitate the application of bacteriocins in the food industry. Full article

Despite being an effective chemotherapeutic agent, the clinical use of doxorubicin (DOX) is limited by several organ toxicities including hepatic injury. Pentoxifylline (PTX) is a methylxanthine derivative with marked anti-inflammatory and anti-apoptotic features. It is unknown, however, whether PTX can mitigate DOX-evoked hepatotoxicity. This study aims to explore the potential hepatoprotective impact of PTX in DOX-induced hepatic injury and the underlying molecular mechanisms. Histopathology, immunohistochemistry, and ELISA were used to examine liver tissues. The current findings revealed that PTX administration to DOX-intoxicated rats mitigated the pathological manifestations of hepatic injury, reduced microscopical damage scores, and improved serum ALT and AST markers, revealing restored hepatic cellular integrity. These favorable effects were attributed to PTX’s ability to mitigate inflammation by reducing hepatic IL-1β and TNF-α levels and suppressing the pro-inflammatory HMGB1/TLR4/NF-κB axis. Moreover, PTX curtailed the hepatic apoptotic abnormalities by suppressing caspase 3 activity and lowering the Bax/Bcl-2 ratio. In tandem, PTX improved the defective autophagy events by lowering hepatic SQSTM-1/p62 accumulation and enhancing the AMPK/mTOR pathway, favoring autophagy and hepatic cell preservation. Together, for the first time, our findings demonstrate the ameliorative effect of PTX against DOX-evoked hepatotoxicity by dampening the hepatic HMGB1/TLR4/NF-κB pro-inflammatory axis and augmenting hepatic AMPK/mTOR-driven autophagy. Thus, PTX could be utilized as an adjunct agent with DOX regimens to mitigate DOX-induced hepatic injury. Full article