Abstract
THE synthesis of the diethyl-ester of pyrocarbonic acid (DEP) was first reported by Boehm and Metha1 in 1938. Later, G. Hecht2 observed that this compound had a strong bactericidal effect. It is also known that DEP rapidly decomposes in water into toxicologically indifferent products; carbon dioxide and alcohol: 
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References
Boehm, T., and Metha, D., Ber. dtsch. chem. Ges., 71, 1797 (1938).
Hecht, G., Zeitschr. f. Lebensm. Unters. Forsch., 114, 242 (1961).
Farbenfabriken Bayer A.G. Baycovin zur Kaltentkeimung alcohol-freier Getränke, Ausgabe vom 1 Juli 1964.
Ivanovics, G., and Csiszár, K., Acta Microbiol. Acad. Sci. Hung., 9, 209 (1962).
Marmur, J., Methods in Enzymology, 6, 726 (1963).
Anagnostopoulus, C., and Spizizen, J., J. Eacteriol., 81, 741 (1961).
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FEDORCSÁK, I., TURTÓCZKY, I. Effects of the Diethyl Ester of Pyrocarbonic Acid on Bacteriophage and Transforming DMA. Nature 209, 830–831 (1966). https://doi.org/10.1038/209830b0
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DOI: https://doi.org/10.1038/209830b0
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