Abstract
VIRUSES, like more complex biological entities, vary in their cryotolerance, their reactions to protective agents, and their resistance to drying by sublimation in vacuo1–3. Temperatures for the preservation of the activities of suspensions of viruses are chosen frequently on the basis of a facility capable of reaching −40° or less, rather than on a comparative study of the effects of temperatures of freezing and storage. Drying from the frozen state by sublimation in vacuo has been used for preparing stable preparations possessing activity after rehydration; the combinations of temperatures of freezing and drying necessary to obtain preparations with maximum activity following rehydration have been investigated less frequently2,4. The investigations recorded here were made to determine the effects of temperatures of freezing, storage at low temperatures and temperatures of drying by sublimation in vacuo on the infectivity titres of suspensions of measles virus. The effects of additives were also investigated.
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References
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GREIFF, D., RIGHTSEL, W. & SCHULER, E. Effects of Freezing, Storage at Low Temperatures, and Drying by Sublimation in vacuo on the Activities of Measles Virus. Nature 202, 624–625 (1964). https://doi.org/10.1038/202624a0
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DOI: https://doi.org/10.1038/202624a0
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