The 2023 MDPI Annual Report has
been released!
 
14 pages, 2173 KiB  
Review
A Probiotic Bacterium with Activity against the Most Frequent Bacteria and Viruses Causing Pediatric Diarrhea: Bifidobacterium longum subsp. infantis CECT 7210 (B. infantis IM1®)
by José Antonio Moreno-Muñoz, Jesús Delgado Ojeda and Jesús Jiménez López
Microorganisms 2024, 12(6), 1183; https://doi.org/10.3390/microorganisms12061183 (registering DOI) - 11 Jun 2024
Abstract
The second leading cause of death in children under five years old is diarrheal disease. Probiotics, specifically bifidobacteria, have been associated with a reduction in the number of diarrhea episodes and their severity in babies. In this paper, we summarize the preclinical and [...] Read more.
The second leading cause of death in children under five years old is diarrheal disease. Probiotics, specifically bifidobacteria, have been associated with a reduction in the number of diarrhea episodes and their severity in babies. In this paper, we summarize the preclinical and clinical evidence of the efficacy of B. longum subsp. infantis IM1® against various gastrointestinal pathogens using in vitro models, animal models, and clinical studies carried out in our laboratory. The preclinical data demonstrate that IM1® effectively inhibits rotavirus replication (by up to 36.05%) in MA-104 and HT-29 cells and from infection (up to 48.50%) through the production of an 11-amino-acid peptide. IM1® displays the capability to displace pathogens from enterocytes, particularly Cronobacter sakazakii and Salmonella enterica, and to reduce the adhesion to the HT29 cells of C. sakazakii and Shigella sonnei. In animal models, the IM1® strain exhibits in vivo protection against rotavirus and improves the clinical symptomatology of bacterial gastroenteritis. A clinical study involving infants under 3 months of age revealed that IM1® reduced episodes of diarrhea, proving to be safe, well tolerated, and associated with a lower prevalence of constipation. B. infantis IM1® emerges as an effective probiotic, diminishing episodes of diarrhea caused by gastrointestinal pathogens. Full article
(This article belongs to the Special Issue Dialogue of Probiotics with the Host 2.0)
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21 pages, 614 KiB  
Review
A Meta-Analysis Review: Nanoparticles as a Gateway to Optimized Boiling Surfaces
by Giulia Motta and Antonis Sergis
Nanomaterials 2024, 14(12), 1012; https://doi.org/10.3390/nano14121012 (registering DOI) - 11 Jun 2024
Abstract
Pool boiling is essential in many industrial manufacturing applications. In addition, it can become critical in the journey towards improving energy generation efficiency and accomplishing the goal of net-zero carbon emissions by 2050 via new or traditional power generation applications. The effectiveness of [...] Read more.
Pool boiling is essential in many industrial manufacturing applications. In addition, it can become critical in the journey towards improving energy generation efficiency and accomplishing the goal of net-zero carbon emissions by 2050 via new or traditional power generation applications. The effectiveness of boiling is governed by the bubble cycle. The chemistry and topographical features of the surface being heated have been found to highly impact the boiling performance, such as in the case of pool boiling enhancement when employing hydrophilic and hydrophobic surfaces via nano/micro heater surface modification. Nevertheless, it is questionable how feasible it is to create these surfaces for large-scale applications due to their manufacturing and maintenance cost and complexity. The current work assesses whether the use of nanoparticles in traditional coolants could potentially unlock the mass production of optimised heating surface modification through a metadata literature review analysis. It was discovered that self-assembled layers created as a result of the deposition of nanoparticles in coolants undergoing pool boiling seem to behave most similarly to manufactured hydrophilic surfaces. The creation of enhanced patterned-heat transfer surfaces is shown to be possible via the use of a combination of different nanoparticle suspensions in coolants. Full article
15 pages, 770 KiB  
Article
The Proximate Composition, Mineral and Pectin Content and Fatty Acid Profile of the Pomace Fraction of 16 Rowanberry Cultivars
by Viive Sarv, Shehzad Hussain, Reelika Rätsep and Ave Kikas
Plants 2024, 13(12), 1615; https://doi.org/10.3390/plants13121615 (registering DOI) - 11 Jun 2024
Abstract
The berry pomace could be a potential source for food applications due to its high content of polyphenols, but also dietary fiber, PUFAs and pectin. This is the first study that aims to compare the total dietary fiber (TDF), protein, fat, mineral, pectin [...] Read more.
The berry pomace could be a potential source for food applications due to its high content of polyphenols, but also dietary fiber, PUFAs and pectin. This is the first study that aims to compare the total dietary fiber (TDF), protein, fat, mineral, pectin and fatty acid content of the following 16 different pomace samples of Sorbus aucuparia L. cultivars (cvs): ‘Likernaja’, ‘Burka’, ‘Alaja Krupnaja’, ‘Granatnaja’, ‘Rubinovaja’, ‘Bussinka’, ‘Vefed’, ‘Angri’, ‘Krasnaja’, ‘Solnechnaja’, ‘Sahharnaja’, ‘Oranzevaja’, ‘Kubovaja’, ‘Moravica’, ‘Rosina’ and ‘Rossica’, in order to find new natural materials for valorization. The contents of pectin and dietary fibers were analyzed using the respective Megazyme enzymatic kits. The TDF content was the highest in the pomace samples of hybrid cvs ‘Granatnaja’ (63.04% dry mass DM), ‘Burka’ (64.52% DM), ‘Rubinovaja’ (65.66% DM) and ‘Likernaja’ (67.17% DM). The pomace of hybrid cv ‘Rubinovaja’ was distinguished from other samples by its high protein content, cv ‘Alaja Krupnaja’ by its high pectin content and cv ‘Oranzevaja’ by its high fat content, which were 7.58% DM, 8.39% DM and 7.47% DM, respectively. The pomace of cv ‘Sahharnaja’ possessed the highest average macro-element content (1.56 g/kg DM). The average fatty acids profile was characterized by a high content of linoleic acid (51.94%), oleic acid (20.55%) and palmitic acid (12.96%). The lowest n6/n3 ratio was found in the hybrid cv ‘Alaja Krupnaja’ (6.70%). The data obtained demonstrate that the pomaces of certain cultivars of rowanberry contain significant amounts of valuable components, which can be used in functional food and cosmetic applications. Full article
22 pages, 4812 KiB  
Article
An Identification Method of Corner Reflector Array Based on Mismatched Filter through Changing the Frequency Modulation Slope
by Le Xia, Fulai Wang, Chen Pang, Nanjun Li, Runlong Peng, Zhiyong Song and Yongzhen Li
Remote Sens. 2024, 16(12), 2114; https://doi.org/10.3390/rs16122114 (registering DOI) - 11 Jun 2024
Abstract
The corner reflector is an effective means of interference for radar seekers due to its high jamming intensity, wide frequency band, and combat effectiveness ratio. Properly arranging multiple corner reflectors in an array can form dilution jamming that resembles ships, substantially enhancing the [...] Read more.
The corner reflector is an effective means of interference for radar seekers due to its high jamming intensity, wide frequency band, and combat effectiveness ratio. Properly arranging multiple corner reflectors in an array can form dilution jamming that resembles ships, substantially enhancing the interference effect. This results in a significant decline in the precision attack efficiency of radar seekers. Hence, it is critical to accurately identify corner reflector array. The common recognition methods involve extracting features on the high-resolution range profile (HRRP) and polarization domain. However, the former is constrained by the number of corner reflectors, while the latter is affected by the accuracy of polarization measurement, both of which have limited performance on the identification of corner reflector array. In terms of the evident variations in physical structures, there must be differences in their scattering characteristics. To highlight the differences, this paper proposes a new method based on the concept of mismatched filtering, which involves changing the frequency modulation slope of the chirp signal in the filter. Then, the variance of width and intervals within a specific scope are extracted as features to characterize these differences, and an identification process is designed in combination with the support vector machine. The simulation experiments demonstrate that the proposed method exhibits stable discriminative performance and can effectively combat dilution jamming. Its accuracy rate exceeds 0.86 when the signal-to-noise ratio is greater than 0 dB. Compared to the HRRP methods, the recognition accuracy of the proposed algorithm improves 15% in relation to variations in the quantity of corner reflectors. Full article
(This article belongs to the Special Issue Remote Sensing of Target Object Detection and Identification II)
16 pages, 1401 KiB  
Systematic Review
How Do Artificial Intelligence, Social Media Platforms and Photo Editing Applications Influence Cosmetic Surgery Choices—Literature Systematic Review and Prospective Study
by Malek Benamor, Stefana Luca, Jed Bouguila, Oxana Madalina Grosu, Bianca Maria Avadani, Dan Cristian Moraru and Mihaela Pertea
Cosmetics 2024, 11(3), 96; https://doi.org/10.3390/cosmetics11030096 (registering DOI) - 11 Jun 2024
Abstract
Background: In recent years, social media and AI have indirectly taken control of our daily lives. We bring attention to the impact that social networks, photo-editing applications, and artificial intelligence have on potential patients when they are looking for a surgeon for a [...] Read more.
Background: In recent years, social media and AI have indirectly taken control of our daily lives. We bring attention to the impact that social networks, photo-editing applications, and artificial intelligence have on potential patients when they are looking for a surgeon for a possible cosmetic surgery, as well as the criteria they consider in relation to the interest in the use of the internet by surgeons. Methods: A systematic review of the past 10 years (2014–2024) was conducted following the PRISMA structure. PubMed and Google Scholar were searched for articles containing the following terms: plastic/esthetic surgery, social media, AI, filters, dysmorphia. All articles were saved using Zotero software version 6.0.37. We reported a prospective study including a 141 patients applying for esthetic surgical interventions in the time interval between February and October 2021. It also involved 44 esthetic surgeons from Tunisian clinics. The influence of social media was evaluated using questionnaires made based on the literature. Results: Using the keyword search, 71 articles were found. A total of 19 articles were selected for data extraction. It was observed that in the last 3 years, the literature has focused on photo-editing and AI in the cosmetic surgery field. A total of 107 patients chose their surgeon based on a surgeon’s social medias rather than their reputation and their website. Conclusions: The increased advancements of the internet have clearly influenced decision making in the field of cosmetic surgery. Full article
(This article belongs to the Special Issue Treatment for Anti-aging and Rejuvenation)
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13 pages, 1235 KiB  
Article
Effect of Metaldehyde on Survival, Enzyme Activities, and Histopathology of the Apple Snail Pomacea canaliculata (Lamarck 1822)
by Jimin Liu, Xuan Chen, Jiaen Zhang, Fucheng Yao, Zhaoji Shi, Yingtong Chen, Qi Chen and Zhong Qin
Biology 2024, 13(6), 428; https://doi.org/10.3390/biology13060428 (registering DOI) - 11 Jun 2024
Abstract
Pomacea canaliculata, as an invasive exotic species in Asia, can adversely affect crop yields, eco-environment, and human health. Application of molluscicides containing metaldehyde is one effective method for controlling P. canaliculata. In order to investigate the effects of metaldehyde on adult snails, [...] Read more.
Pomacea canaliculata, as an invasive exotic species in Asia, can adversely affect crop yields, eco-environment, and human health. Application of molluscicides containing metaldehyde is one effective method for controlling P. canaliculata. In order to investigate the effects of metaldehyde on adult snails, we conducted acute toxicological experiments to investigate the changes in enzyme activities and histopathology after 24 h and 48 h of metaldehyde action. The results showed that the median lethal concentrations (LC) of metaldehyde on P. canaliculata were 3.792, 2.195, 1.833, and 1.706 mg/L at exposure times of 24, 48, 72, and 96 h, respectively. Treatment and time significantly affected acetylcholinesterase (AChE), glutathione S-transferase (GST), and total antioxidant capacity (TAC) activity, with sex significantly affecting AChE, GST, and TAC activity and time significantly affecting carboxylesterase (CarE). In addition, the interaction of treatment and time significantly affected the activity of GST, CarE and TAC. In addition, histopathological changes occurred in the digestive glands, gills and gastropods of apple snail exposed to metaldehyde. Histological examination of the digestive glands included atrophy of the digestive cells, widening of the hemolymph gap, and an increase in basophils. In treated snails, the hemolymph gap in the gills was widely dilated, the columnar cells were disorganized or even necrotic, and the columnar muscle cells in the ventral foot were loosely arranged and the muscle fibers reduced. The findings of this study can provide some references for controlling the toxicity mechanism of invasive species. Full article
15 pages, 1966 KiB  
Article
Assessing the Impact of King Coconut Husk Ash and Biochar, Combined with Chemical Fertilizer Application, on Enhancing Soil Fertility in Coconut Plantations
by Selvaraja Kaushalya Shamila, Shashi S. Udumann, Nuwandhya S. Dissanayaka, Kowshalya Rajaratnam and Anjana J. Atapattu
Crops 2024, 4(2), 227-241; https://doi.org/10.3390/crops4020017 (registering DOI) - 11 Jun 2024
Abstract
Sustainable soil fertility management is crucial for enhancing productivity in coconut plantations. This study investigated the synergistic effects of king coconut husk (KCH) ash, biochar, and chemical fertilizers on soil properties in a coconut plantation over a short period (4 months). Six treatments [...] Read more.
Sustainable soil fertility management is crucial for enhancing productivity in coconut plantations. This study investigated the synergistic effects of king coconut husk (KCH) ash, biochar, and chemical fertilizers on soil properties in a coconut plantation over a short period (4 months). Six treatments were applied: control, chemical fertilizers alone (F), fertilizers with ash (FA), fertilizers with biochar (FB), fertilizers with both ash and biochar (FAB), and fertilizers with half ash and biochar (FA1/2B). Strongly alkaline KCH ash contained significantly higher total and available potassium content levels than mildly alkaline biochar. Data indicated that KCH ash significantly enhanced soil available potassium, electrical conductivity, and organic carbon content compared to the control and F treatments over a short-term period. Even though biochar application demonstrated initial improvements in soil moisture content, a longer study duration may be required to evaluate its influence on other soil parameters comprehensively. Highlighting the synergistic benefits of KCH ash and biochar, FA1/2B treatment exhibited the highest combined index score based on physical, biological, and chemical soil indicators, suggesting its potential for optimizing agricultural outcomes. It emerged as the most promising approach, underscoring the value of exploring sustainable soil amendments derived from agricultural waste streams to promote soil fertility and sustainable coconut production. Full article
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16 pages, 821 KiB  
Article
TPoison: Data-Poisoning Attack against GNN-Based Social Trust Model
by Jiahui Zhao, Nan Jiang, Kanglu Pei, Jie Wen, Hualin Zhan and Ziang Tu
Mathematics 2024, 12(12), 1813; https://doi.org/10.3390/math12121813 (registering DOI) - 11 Jun 2024
Abstract
In online social networks, users can vote on different trust levels for each other to indicate how much they trust their friends. Researchers have improved their ability to predict social trust relationships through a variety of methods, one of which is the graph [...] Read more.
In online social networks, users can vote on different trust levels for each other to indicate how much they trust their friends. Researchers have improved their ability to predict social trust relationships through a variety of methods, one of which is the graph neural network (GNN) method, but they have also brought the vulnerability of the GNN method into the social trust network model. We propose a data-poisoning attack method for GNN-based social trust models based on the characteristics of social trust networks. We used a two-sample test for power-law distributions of discrete data to avoid changes in the dataset being detected and used an enhanced surrogate model to generate poisoned samples. We further tested the effectiveness of our approach on three real-world datasets and compared it with two other methods. The experimental results using three datasets show that our method can effectively avoid detection. We also used three metrics to illustrate the effectiveness of our attack, and the experimental results show that our attack stayed ahead of the other two methods in all three datasets. In terms of one of our metrics, our attack method decreased the accuracies of the attacked models by 12.6%, 22.8%, and 13.8%. Full article
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21 pages, 2329 KiB  
Review
Chicken Egg White Gels: Fabrication, Modification, and Applications in Foods and Oral Nutraceutical Delivery
by Junhua Li, Xuechun Wang, Cuihua Chang, Luping Gu, Yujie Su, Yanjun Yang, Dominic Agyei and Qi Han
Foods 2024, 13(12), 1834; https://doi.org/10.3390/foods13121834 (registering DOI) - 11 Jun 2024
Abstract
Chicken egg white (EW) proteins possess various useful techno-functionalities, including foaming, gelling or coagulating, and emulsifying. The gelling property is one of the most important functionalities of EW proteins, affecting their versatile applications in the food and pharmaceutical industries. However, it is challenging [...] Read more.
Chicken egg white (EW) proteins possess various useful techno-functionalities, including foaming, gelling or coagulating, and emulsifying. The gelling property is one of the most important functionalities of EW proteins, affecting their versatile applications in the food and pharmaceutical industries. However, it is challenging to develop high-quality gelled foods and innovative nutraceutical supplements using native EW and its proteins. This review describes the gelling properties of EW proteins. It discusses the development and action mechanism of the physical, chemical, and biological methods and exogenous substances used in the modification of EW gels. Two main applications of EW gels, i.e., gelling agents in foods and gel-type carriers for nutraceutical delivery, are systematically summarized and discussed. In addition, the research and technological gaps between modified EW gels and their applications are highlighted. By reviewing the new modification strategies and application trends of EW gels, this paper provides insights into the development of EW gel-derived products with new and functional features. Full article
(This article belongs to the Section Nutraceuticals, Functional Foods, and Novel Foods)
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18 pages, 3024 KiB  
Article
Plant Growth Promotion and Biological Control against Rhizoctonia solani in Thai Local Rice Variety “Chor Khing” Using Trichoderma breve Z2-03
by Warin Intana, Nakarin Suwannarach, Jaturong Kumla, Prisana Wonglom and Anurag Sunpapao
J. Fungi 2024, 10(6), 417; https://doi.org/10.3390/jof10060417 (registering DOI) - 11 Jun 2024
Abstract
Several strains of Trichoderma are applied in the field to control plant diseases due to their capacity to suppress fungal pathogens and control plant diseases. Some Trichoderma strains also are able to promote plant growth through the production of indole-3-acetic acid (IAA). In [...] Read more.
Several strains of Trichoderma are applied in the field to control plant diseases due to their capacity to suppress fungal pathogens and control plant diseases. Some Trichoderma strains also are able to promote plant growth through the production of indole-3-acetic acid (IAA). In southern Thailand, the local rice variety “Chor Khing” is mainly cultivated in the Songkhla province; it is characterized by slow growth and is susceptible to sheath blight caused by Rhizoctonia solani. Therefore, this research aimed to screen Trichoderma species with the ability to promote plant growth in this rice variety and enact biological control against R. solani. A total of 21 Trichoderma isolates were screened for indole compound production using the Salkowski reagent. The Z2-03 isolate reacted positively to the Salkowski reagent, indicating the production of the indole compound. High-performance liquid chromatography (HPCL) confirmed that Z2-03 produced IAA at 35.58 ± 7.60 μg/mL. The cell-free culture filtrate of the potato dextrose broth (CF) of Z2-03 induced rice germination in rice seeds, yielding root and shoot lengths in cell-free CF-treated rice that were significantly higher than those of the control (distilled water and culture broth alone). Furthermore, inoculation with Trichoderma conidia promoted rice growth and induced a defense response against R. solani during the seedling stage. Trichoderma Z2-03 displayed an antifungal capacity against R. solani, achieving 74.17% inhibition (as measured through dual culture assay) and the production of siderophores on the CAS medium. The pot experiment revealed that inoculation with the Trichoderma sp. Z2-03 conidial suspension increased the number of tillers and the plant height in the “Chor Khing” rice variety, and suppressed the percentage of disease incidence (PDI). The Trichoderma isolate Z2-03 was identified, based on the morphology and molecular properties of ITS, translation elongation factor 1-alpha (tef1-α), and RNA polymerase 2 (rpb2), as Trichoderma breve Z2-03. Our results reveal the ability of T. breve Z2-03 to act as a plant growth promoter, enhancing growth and development in the “Chor Khing” rice variety, as well as a biological control agent through its competition and defense induction mechanism in this rice variety. Full article
(This article belongs to the Special Issue Soil Fungi and Their Role in Plant Growth)
27 pages, 18961 KiB  
Article
Driving Factors and Trade-Offs/Synergies Analysis of the Spatiotemporal Changes of Multiple Ecosystem Services in the Han River Basin, China
by Peidong Han, Guang Yang, Zijun Wang, Yangyang Liu, Xu Chen, Wei Zhang, Zhixin Zhang, Zhongming Wen, Haijing Shi, Ziqi Lin and Hanyu Ren
Remote Sens. 2024, 16(12), 2115; https://doi.org/10.3390/rs16122115 (registering DOI) - 11 Jun 2024
Abstract
 Uncovering the trade-offs and synergy relationship of multiple ecosystem services (ESs) is important for scientific ecosystem management and the improvement of ecological service functions. In this study, we investigated the spatiotemporal changes of four typical ES types (i.e., water yield (WY), carbon storage [...] Read more.
 Uncovering the trade-offs and synergy relationship of multiple ecosystem services (ESs) is important for scientific ecosystem management and the improvement of ecological service functions. In this study, we investigated the spatiotemporal changes of four typical ES types (i.e., water yield (WY), carbon storage (CS), soil conservation (SC), and habitat quality (HQ)) from 2001 to 2020 in the Han River basin (HRB). Meanwhile, the trade-offs and synergies between paired ESs and the socioecological drivers of these ESs were further explored. The results showed that grassland, cropland, and bare land decreased by 12,141.3 km2, 624.09 km2, and 22.1 km2 during the study period, respectively, which can be attributed to their conversion to forests in the HRB. Temporally, the WY, CS, and SC all showed a continuously increasing trend. Spatially, WY and HQ exhibited bipolar clustering characteristics, with WY exhibiting low-value clustering in the upstream and high-value clustering in the downstream, while CS showed the clustering characteristics of a scattered distribution of cold and hot spots from 2001 to 2020. The spatial patterns of aggregation locations in CS and HQ were relatively similar, with clusters of higher ES values mainly distributed in the western and central regions and clusters of lower ES values mainly located in the eastern and southeastern regions, while the aggregation of WY was spatially concentrated. Overall, the CS showed a significant positive correlation with HQ, but a significant negative correlation with WY. Spatially, WY and HQ, CS, and SC showed a substantial trade-off relationship in the northwest and southeast parts of the study area, while HQ, CS, and SC mainly exhibited a synergistic relationship in most parts of the study area. Slope and temperature had high influencing factor coefficients on multiple ESs; the mixed effect of terrain and natural factors was significantly greater than the impact of a single factor on ESs, and terrain factors played an essential role in the changes in ESs. The findings can provide technical and theoretical support for integrated scientific ecosystem management and sustainable development at the local scale.  Full article
(This article belongs to the Special Issue Assessment of Ecosystem Services Based on Satellite Data)
26 pages, 4539 KiB  
Systematic Review
Effects of Weight Loss on Key Obesity-Related Biomarkers Linked to the Risk of Endometrial Cancer: A Systematic Review and Meta-Analysis
by Angela D. Clontz, Emma Gan, Stephen D. Hursting and Victoria L. Bae-Jump
Cancers 2024, 16(12), 2197; https://doi.org/10.3390/cancers16122197 (registering DOI) - 11 Jun 2024
Abstract
Endometrial cancer (EC) includes various histologic types, with estrogen-dependent endometrioid carcinoma being the most common. Obesity significantly increases the risk of developing this type, especially in postmenopausal women, due to elevated estrogen production by adipocytes. This review examines the impact of weight loss [...] Read more.
Endometrial cancer (EC) includes various histologic types, with estrogen-dependent endometrioid carcinoma being the most common. Obesity significantly increases the risk of developing this type, especially in postmenopausal women, due to elevated estrogen production by adipocytes. This review examines the impact of weight loss from different interventions on reducing obesity-related risk factors for endometrioid EC. A systematic review and meta-analysis were conducted on three weight loss interventions: bariatric surgery, pharmacotherapy, and lifestyle changes. The effects of these interventions on inflammatory biomarkers (CRP, TNF-α, IL-6) and hormones (leptin, estrogen) were analyzed. Data from controlled studies were pooled to assess the significance of weight loss in reducing these biomarkers. Despite heterogeneity, bariatric surgery resulted in an overall 25.8% weight reduction, outperforming lifestyle and pharmacotherapy interventions. Weight loss reduced CRP levels by 33.5% and IL-6 levels by 41.9%. TNF-α levels decreased by 13% with percent weight loss over 7%. Leptin levels also decreased significantly, although the exact weight loss percentage was not statistically significant. Weight loss effectively reduces proinflammatory markers and hormones associated with increased risk of endometrioid EC. The strengths of this review include a comprehensive examination of different weight-loss interventions and a large pool of participants. However, limitations include high heterogeneity among studies and only 43% of the participants being postmenopausal. Limited data on sex hormones and racial disparities underscore the need for further research. Full article
(This article belongs to the Special Issue Gynecologic Cancer: Risk Factors, Interception and Prevention)
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18 pages, 16396 KiB  
Article
Degradation of Proteoglycans and Collagen in Equine Meniscal Tissues
by Julia Dubuc, Melodie Jil Schneider, Valerie Dubuc, Helene Richard, Maxime Pinsard, Stephane Bancelin, Francois Legare, Christiane Girard and Sheila Laverty
Int. J. Mol. Sci. 2024, 25(12), 6439; https://doi.org/10.3390/ijms25126439 (registering DOI) - 11 Jun 2024
Abstract
Investigate meniscal extracellular matrix degradation. Equine menisci (n = 34 from 17 horses) were studied. Site-matched sections were cut and scored from three regions (ROIs; n = 102) and stained for histology, proteoglycan (safranin O and fast green), aggrecan, and collagen cleavage [...] Read more.
Investigate meniscal extracellular matrix degradation. Equine menisci (n = 34 from 17 horses) were studied. Site-matched sections were cut and scored from three regions (ROIs; n = 102) and stained for histology, proteoglycan (safranin O and fast green), aggrecan, and collagen cleavage (NITEGE, DIPEN, and C1,2C antibodies, respectively). Picrosirius red and second harmonic generation microscopy were performed to investigate collagen ultrastructure. A total of 42 ROIs met the inclusion criteria and were included in the final analysis. The median (range) ROI histological score was 3 (0–9), providing a large spectrum of pathology. The median (range) proteoglycan score was 1 (0–3), representing superficial and central meniscal loss. The median (range) of DIPEN, NITEGE, and C1,2C scores was 1 (0–3), revealing immunostaining of the femoral and tibial surfaces. The proteoglycan scores exhibited significant positive associations with both histologic evaluation (p = 0.03) and DIPEN scores (p = 0.02). Additionally, a robust positive association (p = 0.007) was observed between the two aggrecanolysis indicators, NITEGE and DIPEN scores. A negative association (p = 0.008) was identified between NITEGE and histological scores. The C1,2C scores were not associated with any other scores. Picrosirius red and second harmonic generation microscopy (SHGM) illustrated the loss of the collagen matrix and structure centrally. Proteoglycan and collagen degradation commonly occur superficially in menisci and less frequently centrally. The identification of central meniscal proteoglycan and collagen degradation provides novel insight into central meniscal degeneration. However, further research is needed to elucidate the etiology and sequence of degradative events. Full article
(This article belongs to the Special Issue New Advances in Osteoarthritis: Molecular Perspective)
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14 pages, 1621 KiB  
Article
Surface Modification of Zr48Cu36Al8Ag8 Bulk Metallic Glass through Glow Discharge Plasma Nitriding
by Krzysztof Kulikowski, Piotr Błyskun, Tomasz Borowski and Tadeusz Kulik
Materials 2024, 17(12), 2850; https://doi.org/10.3390/ma17122850 (registering DOI) - 11 Jun 2024
Abstract
Bulk metallic glasses are modern engineering materials with unique functional properties. Zr-based alloys are particularly attractive as they exhibit high glass forming ability as well as good mechanical properties. Due to their relatively high thermal stability, reaching as much as 400 °C, they [...] Read more.
Bulk metallic glasses are modern engineering materials with unique functional properties. Zr-based alloys are particularly attractive as they exhibit high glass forming ability as well as good mechanical properties. Due to their relatively high thermal stability, reaching as much as 400 °C, they can be surface-treated in low-temperature plasma to further improve their mechanical properties. The subject of this study was to determine the influence of the technological parameters of nitriding in low-temperature plasma on the structure and mechanical properties of Zr48Cu36Al8Ag8 bulk metallic glass. In the course of this study, the influence of the ion accelerating voltage on the structure and micromechanical properties of the bulk metallic glass was analyzed. The produced samples were characterized in terms of nanohardness, layer adhesion by using the scratch test, and wear resistance by using the ball-on-disc method. As a result of low-temperature plasma nitriding, a significant increase in the surface nanohardness of the Zr48Cu36Al8Ag8 bulk metallic glass was obtained. The produced layers exhibited high adhesion to the substrate and they improved the wear resistance of the glass. The present study indicates the possibility of modifying the surface properties of bulk metallic glasses by using diffusion processes in low-temperature plasma without substrate crystallization. Full article
(This article belongs to the Special Issue Structure and Properties of Metallic Glasses)
9 pages, 491 KiB  
Communication
The Development of a Rapid, Cost-Effective, and Green Analytical Method for Mercury Speciation
by Patrícia Cristina Costa Ladeira, Caroline Cristine Augusto, Bruno Alves Rocha, Jairo Lisboa Rodrigues, Giovanna de Fátima Moreno Aguiar and Bruno Lemos Batista
Toxics 2024, 12(6), 424; https://doi.org/10.3390/toxics12060424 (registering DOI) - 11 Jun 2024
Abstract
Mercury is a naturally occurring metal found in various inorganic and organic forms within the environment. Due to its high toxicity, there is global concern regarding human exposure to this element. The combination of high-performance liquid chromatography and inductively coupled plasma mass spectrometry [...] Read more.
Mercury is a naturally occurring metal found in various inorganic and organic forms within the environment. Due to its high toxicity, there is global concern regarding human exposure to this element. The combination of high-performance liquid chromatography and inductively coupled plasma mass spectrometry (HPLC-ICP-MS) is commonly used to analyze the different forms of mercury in a sample due to its high sensitivity and ability to selectively detect mercury. However, the traditional HPLC-ICP-MS methods are often criticized for their lengthy analysis times. In this study, we have refined the conventional approach by transitioning to ultra-high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (UHPLC-ICP-MS). This modification has resulted in significant reductions in runtime as well as reagent and argon usage, thereby offering a more rapid, environmentally friendly, and cost-effective method. We successfully adapted an HPLC-ICP-MS method to UHPLC-ICP-MS, achieving the analysis of Hg2+ and MeHg+ within 1 min with a mobile phase consumption of only 0.5 mL and a sample volume of 5.0 µL; this is a major advance compared to HPLC analysis with run times generally between 5 and 10 min. The method’s performance was assessed by analyzing muscle and liver tissue samples (serving as reference material) from fish, demonstrating the versatility of the method in relation to different complex matrices. Full article
(This article belongs to the Section Novel Methods in Toxicology Research)
11 pages, 609 KiB  
Article
Genetic Heterogeneity in Cowpea Genotypes (Vigna unguiculata L. Walp) Using DArTseq (GBS)-Derived Single Nucleotide Polymorphisms
by Goitsemang Mahlomola Hendry Dikane and Moosa Mahmood Sedibe
Genes 2024, 15(6), 764; https://doi.org/10.3390/genes15060764 (registering DOI) - 11 Jun 2024
Abstract
Cowpeas (Vigna unguiculata L. Walp) have been credible constituents of nutritious food and forage in human and animal diets since the Neolithic era. The modern technique of Diversity Array Technology (DArTseq) is both cost-effective and rapid in producing thousands of high-throughputs, genotyped, [...] Read more.
Cowpeas (Vigna unguiculata L. Walp) have been credible constituents of nutritious food and forage in human and animal diets since the Neolithic era. The modern technique of Diversity Array Technology (DArTseq) is both cost-effective and rapid in producing thousands of high-throughputs, genotyped, single nucleotide polymorphisms (SNPs) in wide-genomic analyses of genetic diversity. The aim of this study was to assess the heterogeneity in cowpea genotypes using DArTseq-derived SNPs. A total of 92 cowpea genotypes were selected, and their fourteen-day-old leaves were freeze-dried for five days. DNA was extracted using the CTAB protocol, genotyped using DArTseq, and analysed using DArTsoft14. A total of 33920 DArTseq-derived SNPs were recalled for filtering analysis, with a final total of 16960 SNPs. The analyses were computed using vcfR, poppr, and ape in R Studio v1.2.5001-3 software. The heatmap revealed that the TVU 9596 (SB26), Orelu (SB72), 90K-284-2 (SB55), RV 403 (SB17), and RV 498 (SB16) genotypes were heterogenous. The mean values for polymorphic information content, observed heterozygosity, expected heterozygosity, major allele frequency, and the inbreeding coefficient were 0.345, 0.386, 0.345, 0.729, and 0.113, respectively. Moreover, they validated the diversity of the evaluated cowpea genotypes, which could be used for potential breeding programmes and management of cowpea germplasm. Full article
(This article belongs to the Special Issue Genetics and Breeding of Legume Crops)
27 pages, 364 KiB  
Article
Follow the Leader: How Culture Gives Rise to a Behavioral Bias That Leads to Higher Greenhouse Gas Emissions
by Le Zhao, Nima Vafai, Marcos Velazquez and Abu Amin
J. Risk Financial Manag. 2024, 17(6), 245; https://doi.org/10.3390/jrfm17060245 (registering DOI) - 11 Jun 2024
Abstract
This research investigates the influence of national culture, particularly power distance, on firms’ carbon dioxide (CO2) emissions. Drawing on a large international dataset spanning over a decade, we examine how power distance, agency conflict, and socioeconomic stability interact to shape firms’ [...] Read more.
This research investigates the influence of national culture, particularly power distance, on firms’ carbon dioxide (CO2) emissions. Drawing on a large international dataset spanning over a decade, we examine how power distance, agency conflict, and socioeconomic stability interact to shape firms’ emission decisions. Our analysis reveals a significant positive relationship between power distance and firms’ CO2 emissions, indicating that firms located in countries characterized by higher power distance tend to emit more greenhouse gases (GHGs). Furthermore, we find that agency conflict moderates this relationship, with firms experiencing high levels of debt or paying substantial dividends exhibiting lower emissions in high power distance environments. Additionally, socioeconomic stability attenuates the positive association between power distance and emissions, suggesting that the effectiveness of cultural influences on emission decisions is contingent upon the stability of the societal context. These findings underscore the importance of considering cultural dimensions, agency dynamics, and socioeconomic conditions in understanding corporate environmental behavior. Our research contributes to the literature by providing empirical evidence of the nuanced interplay between national culture, agency conflict, and socioeconomic stability in shaping firms’ emission decisions. Policymakers and practitioners can use these insights to develop more targeted environmental policies and strategies aimed at promoting sustainable development globally. Full article
(This article belongs to the Special Issue Quantitative Finance in Energy)
16 pages, 353 KiB  
Article
Effectiveness and Safety of the MVA–BN Vaccine against Mpox in At-Risk Individuals in the United States (USMVAc)
by Soowoo Back, Bethany Knox, Ciara Coakley, Nicolas Deltour, Emmanuelle Jacquot, Hanaya Raad and Elizabeth M. Garry
Vaccines 2024, 12(6), 651; https://doi.org/10.3390/vaccines12060651 (registering DOI) - 11 Jun 2024
Abstract
The mpox 2022 outbreak was declared a public health emergency in July 2022. In August 2022, the MVA–BN vaccine received emergency use authorization in the United States (US) to target at-risk groups. This study (EUPAS104386) used HealthVerity’s administrative US healthcare data to generate [...] Read more.
The mpox 2022 outbreak was declared a public health emergency in July 2022. In August 2022, the MVA–BN vaccine received emergency use authorization in the United States (US) to target at-risk groups. This study (EUPAS104386) used HealthVerity’s administrative US healthcare data to generate real-world evidence for MVA–BN vaccine effectiveness and safety to prevent mpox disease in men who have sex with men (MSM) and transgender women, the most affected population during the 2022 mpox outbreak. Fully vaccinated subjects (two doses ≥ 28 days apart) were initially matched with five unvaccinated subjects on calendar date, age, US region, and insurance type. Subjects were followed from index date (14 days after the second dose) until death or data end to ascertain mpox occurrence. After propensity score adjustment, the MVA–BN vaccine effectiveness against mpox disease was 89% (95% CI: 12%, 99%) among those fully vaccinated; attenuated to 64% (95% CI: 40%, 78%) among those with any dose and 70% (95% CI: 44%, 84%) for those with only a single dose. One pericarditis adverse event of special interest was observed when the risk window was extended to 28 days. These results contribute to the totality of evidence supporting the favorable benefit/risk profile of the MVA–BN vaccine. Full article
(This article belongs to the Section Vaccine Efficacy and Safety)
16 pages, 1540 KiB  
Article
Research on the Effects of Different Environmental Regulation Tools on China’s Industrial Water Green Use Efficiency—Comparison between the Yellow River Basin and the Yangtze River Economic Belt
by Yuchun Yang, Shanni Liu and Muhammad Kamran Khan
Sustainability 2024, 16(12), 4984; https://doi.org/10.3390/su16124984 (registering DOI) - 11 Jun 2024
Abstract
Improving industrial water green use efficiency (IWGUE) is a primary means to ensure the production, living, and ecological use of water quantity and quality, while effective environmental regulation tools are important to promote efficiency. This paper calculates the industrial water green use efficiency [...] Read more.
Improving industrial water green use efficiency (IWGUE) is a primary means to ensure the production, living, and ecological use of water quantity and quality, while effective environmental regulation tools are important to promote efficiency. This paper calculates the industrial water green use efficiency in China’s 30 provinces from 2010 to 2022 by the SE-SBM model and divides environmental regulatory tools into command-based, market-oriented, and voluntary types. The panel Tobit model is constructed to test the impact and differences in the effects of three environmental regulations on regional industrial water green use efficiency. The results show the following: (1) Under the constraint of undesired output, IWGUE fluctuates upward slowly in China, and the potential for improving the efficiency value is enormous, with significant regional and basin-level differences. (2) At the national level, the impact of command-based and market-oriented environmental regulations on IWGUE shows a U-shaped trend, while the positive promoting effect of voluntary environmental regulations on efficiency is not significant. (3) In the Yellow River Basin, the impact of three types of environmental regulations on IWGUE shows a U-shaped pattern. Command-based and voluntary environmental regulations have crossed the inflection point and have a significant promoting effect on efficiency, while market-oriented environmental regulations have not yet crossed the inflection point. (4) In the Yangtze River Economic Belt, the impact of command-based and market-oriented environmental regulations on IWGUE shows a U-shaped pattern, while voluntary environmental regulations have a significant promoting effect on efficiency. This study may provide a reference for tailored policy design to improve industrial water efficiency in China from the perspective of environmental regulations. Full article
19 pages, 581 KiB  
Article
Ultrasound-Guided Greater Ischiatic Notch Plane Block Combined with the Caudal Quadratus Lumborum Block (GIN-TONIC Block) in Dogs Undergoing Pelvic Limb Surgery: Preliminary Results
by Pablo E. Otero, Jorge A. Guerrero, Lisa Tarragona, Fabiana Micieli, María Fernanda Sanchez, Pablo A. Donati, Martin R. Ceballos and Diego A. Portela
Animals 2024, 14(12), 1764; https://doi.org/10.3390/ani14121764 (registering DOI) - 11 Jun 2024
Abstract
This study assessed the analgesic and motor effects of the GIN-TONIC block, a combination of the greater ischiatic notch plane block and the caudal lateral quadratus lumborum block, in 24 dogs undergoing pelvic limb surgery. Dogs were randomly divided into two equal groups: [...] Read more.
This study assessed the analgesic and motor effects of the GIN-TONIC block, a combination of the greater ischiatic notch plane block and the caudal lateral quadratus lumborum block, in 24 dogs undergoing pelvic limb surgery. Dogs were randomly divided into two equal groups: GA received acepromazine [(20 µg kg–1 intravenously (IV)] as premedication, and GD received dexmedetomidine (2 µg kg–1 IV). General anesthesia was maintained with isoflurane, and both groups received a GIN-TONIC block using 2% lidocaine. Nociception during surgery and postoperative pain [assessed using the Glasgow Composite Measure Pain Score (GCMPS-SF)] were assessed. Fentanyl (2 µg kg–1 IV) was administered if nociception was noted and morphine (0.5 mg kg–1 IV) was administered during recovery if the pain scores exceeded the predefined threshold. Motor function was assessed during the recovery period using descriptors previously reported. All dogs received analgesics at the 4 h mark before being discharged. Three and two dogs in GD and GA required fentanyl once. Postoperative pain scores remained ≤4/20 for all dogs except one. Dogs achieved non-ataxic ambulation within 38.9 ± 10.3 and 35.1 ± 11.1 min after extubation in GD and GA, respectively. This study highlighted the potential of the GIN-TONIC block as a feasible regional anesthesia method for delivering perioperative analgesia in dogs undergoing pelvic limb orthopedic surgery. Full article
(This article belongs to the Special Issue Second Edition: Loco-Regional Anaesthesia in Veterinary Medicine)
17 pages, 296 KiB  
Article
Impact of Effort–Reward Imbalance and Burnout on the Compliance with Standard Precautions among Nurses and Midwives in Lebanese Hospitals
by Noha A. Sayrafi, Ali Salami and Ghassan Ghssein
Nurs. Rep. 2024, 14(2), 1477-1493; https://doi.org/10.3390/nursrep14020111 (registering DOI) - 11 Jun 2024
Abstract
Background: Effort–reward imbalance (ERI) refers to the situation where there is a mismatch between the effort that healthcare workers (HCWs) put into their work and the rewards they receive in return. Burnout, on the other hand, is a psychological syndrome characterized by emotional [...] Read more.
Background: Effort–reward imbalance (ERI) refers to the situation where there is a mismatch between the effort that healthcare workers (HCWs) put into their work and the rewards they receive in return. Burnout, on the other hand, is a psychological syndrome characterized by emotional exhaustion, depersonalization, and reduced personal accomplishment. This study aimed to assess the impact of ERI and burnout on the compliance with standard precautions (SPs) among nurses and midwives in Lebanese hospitals under the economic crisis and after the COVID-19 pandemic. Methods: Cross-sectional correlational study, based on self-administered questionnaire data, measuring the compliance with SPs, ERI, and burnout, in addition to the relationship between these factors, was performed among 409 nurses and midwives, working in Lebanese hospitals. Results: ERI was significantly associated with exposure to burnout among nurses and midwives, and burnout was found to be a significant predictor of nurses and midwives’ self-reported adherence with SPs. Conclusions: This study highlights the contribution of ERI and burnout to the chain of infection by decreased adherence to infection control SPs of nurses and midwives. Full article
(This article belongs to the Special Issue Burnout and Nursing Care)
8 pages, 860 KiB  
Article
Changes in Maxillary Sinus Volume and Mucosal Thickness Post Bimaxillary Advancement Procedures: A Retrospective Study
by Paweł Piotr Grab, Michał Szałwiński, Piotr Rot, Aldona Chloupek, Maria Sobol and Dariusz Jurkiewicz
J. Clin. Med. 2024, 13(12), 3425; https://doi.org/10.3390/jcm13123425 (registering DOI) - 11 Jun 2024
Abstract
Background: Bimaxillary surgery is an elemental procedure in the field of cranio-maxillofacial surgery. It allows for the correction of even the most challenging cases of maxillomandibular disorders, malocclusion, facial asymmetry, and disproportion. The osteotomies and maneuvers carried out during the procedure result in [...] Read more.
Background: Bimaxillary surgery is an elemental procedure in the field of cranio-maxillofacial surgery. It allows for the correction of even the most challenging cases of maxillomandibular disorders, malocclusion, facial asymmetry, and disproportion. The osteotomies and maneuvers carried out during the procedure result in changes to the surrounding tissues, including the maxillary sinuses (MS). The aim of this study was to assess the change in the maxillary sinus volume and the thickness of the mucosa after maxillomandibular advancement (MMA) surgeries. Methods: A group of 25 patients who underwent MMA surgery were included in the study. Computed tomography (CT) of the head and neck region was performed 2 weeks preoperatively and 6 months postoperatively. Acquired Digital Imaging and Communications in Medicine (DICOM) files were analyzed using different software programs to calculate the medium MS mucosa thickness and MS volume. Results: A statistically significant reduction in MS volume was observed (p = 0.015). The change in the median thickness of the MS mucosa was not statistically significant. The median sella-nasion-A point angle (SNA angle) value of the group increased from 80.2 to 83.4 degrees. A weak negative correlation between the SNA delta and the MS volume delta was observed. Spearman’s rank coefficient: (ρ s = −0.381, p = 0.060). Conclusions: The MMA surgery results in a reduction in the MS volume. The amount of forward movement of the maxilla may be correlated with the extent of the MS volume reduction. Full article
(This article belongs to the Section Otolaryngology)
20 pages, 1571 KiB  
Review
Progress and Prospects of Research on the Impact of Forest Therapy on Mental Health: A Bibliometric Analysis
by Ruoyu Wan, Ruohong Wan and Quan Qiu
Forests 2024, 15(6), 1013; https://doi.org/10.3390/f15061013 (registering DOI) - 11 Jun 2024
Abstract
This study aims to assess the global progress in research on the impact of forest therapy on mental health by using bibliometric analysis. We analyzed the relevant literature from the Web of Science Core Collection (WoSCC) database by utilizing software tools such as [...] Read more.
This study aims to assess the global progress in research on the impact of forest therapy on mental health by using bibliometric analysis. We analyzed the relevant literature from the Web of Science Core Collection (WoSCC) database by utilizing software tools such as Bibliometrix (Version 4.1), VOSviewer (Version 1.6.19), and CiteSpace (Version 6.3.R1). There appears to be a growing trend in annual publication volumes from 2006 to 2023. Miyazaki was identified as the most prolific author in this field. Japan and South Korea lead in research contributions, while China has the highest number of publications. The International Journal of Environmental Research and Public Health is the journal with the most publications and citations in this area. Recent keyword clustering reveals an increasing frequency of studies on anxiety and urban green spaces. Research findings suggest that exposure to negative air ions and compounds released by plants can stimulate the five human senses, reduce cortisol levels, and improve mental health. Although studies have demonstrated significant effects of forest therapy in alleviating stress, anxiety, and depression, more large-scale and long-term studies are needed for further evidence. Additionally, it is crucial to explore the impact of different forest types, terrains, altitudes, climates, and air conditions on the effectiveness of forest therapy on mental health. Full article
(This article belongs to the Section Forest Economics, Policy, and Social Science)

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