The 2023 MDPI Annual Report has
been released!
 
11 pages, 1291 KiB  
Article
Comprehensive Approach to Phenotype Varroa destructor Reproduction in Honey Bee Drone Brood and Its Correlation with Decreased Mite Reproduction (DMR)
by Regis Lefebre, David Claeys Bouuaert, Emma Bossuyt, Lina De Smet, Marleen Brunain, Ellen Danneels and Dirk C. de Graaf
Insects 2024, 15(6), 397; https://doi.org/10.3390/insects15060397 (registering DOI) - 29 May 2024
Abstract
The mechanisms of action behind decreased mite reproduction (DMR) are still unknown, but current hypotheses state that DMR is the result of brood-intrinsic and/or external disturbances in the V. destructor—honey bee pupa signal interactions. For accurate and precise DMR phenotyping, sufficient single [...] Read more.
The mechanisms of action behind decreased mite reproduction (DMR) are still unknown, but current hypotheses state that DMR is the result of brood-intrinsic and/or external disturbances in the V. destructor—honey bee pupa signal interactions. For accurate and precise DMR phenotyping, sufficient single infested honey bee brood cells are required (e.g., 35), which requires extensive labor and time and may exclude many samples not reaching the threshold. We defined a new comprehensive trait called the ‘mean V. destructor reproduction rate’ (mVR), which describes the mean number of offspring mites per infested cell in the sample while compensating for the reduced number of offspring with increasing multiple infested cells. We found a significant correlation between mVR and DMR, allowing for an estimation of DMR based on the mVR only. When the mVR was calculated with 10 infested cells, we found an average variation in mVR of 16.8%. For the same variation in DMR determination, 40 single infested cells are required. This broader look at V. destructor resistance phenotyping can improve the applicability and effectiveness of traits related to V. destructor reproduction in honey bee breeding programs. Full article
(This article belongs to the Special Issue Healthy and Sustainable Beekeeping)
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11 pages, 4818 KiB  
Article
Synthesis and Electrocatalytic Performance Study of Sulfur Quantum Dots Modified MoS2
by Guiyu Wei, Tao Tang, Ruizheng Xu, Zhemin Xie, Sijie Diao, Jianfeng Wen, Li Jiang, Guanghui Hu and Ming Li
Molecules 2024, 29(11), 2551; https://doi.org/10.3390/molecules29112551 (registering DOI) - 29 May 2024
Abstract
The electrolysis of water for hydrogen production is currently receiving significant attention due to its advantageous features such as non-toxicity, safety, and environmental friendliness. This is especially crucial considering the urgent need for clean energy. However, the current method of electrolyzing water to [...] Read more.
The electrolysis of water for hydrogen production is currently receiving significant attention due to its advantageous features such as non-toxicity, safety, and environmental friendliness. This is especially crucial considering the urgent need for clean energy. However, the current method of electrolyzing water to produce hydrogen largely relies on expensive metal catalysts, significantly increasing the costs associated with its development. Molybdenum disulfide (MoS2) is considered the most promising alternative to platinum for electrocatalyzing the hydrogen evolution reaction (HER) due to its outstanding catalytic efficiency and robust stability. However, the practical application of this material is hindered by its low conductivity and limited exposure of active sites. MoS2/SQDs composite materials were synthesized using a hydrothermal technique to deposit SQDs onto MoS2. These composite materials were subsequently employed as catalysts for the HER. Research findings indicate that incorporating SQDs can enhance electron transfer rates and increase the active surface area of MoS2, which is crucial for achieving outstanding catalytic performance in the HER. The MoS2/SQDs electrocatalyst exhibits outstanding performance in the HER when tested in a 0.5 M H2SO4 solution. It achieves a remarkably low overpotential of 204 mV and a Tafel slope of 65.82 mV dec−1 at a current density of 10 mA cm−2. Moreover, during continuous operation for 24 h, the initial current density experiences only a 17% reduction, indicating high stability. This study aims to develop an efficient and cost-effective electrocatalyst for water electrolysis. Additionally, it proposes a novel design strategy that uses SQDs as co-catalysts to enhance charge transfer in nanocomposites. Full article
(This article belongs to the Section Electrochemistry)
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19 pages, 33144 KiB  
Article
Performance Analysis of Helical Milling and Drilling Operations While Machining Carbon Fiber-Reinforced Aluminum Laminates
by Gururaj Bolar, Anoop Aroor Dinesh, Ashwin Polishetty, Raviraj Shetty, Anupama Hiremath and V. L. Neelakantha
J. Manuf. Mater. Process. 2024, 8(3), 113; https://doi.org/10.3390/jmmp8030113 (registering DOI) - 29 May 2024
Abstract
Being a difficult-to-cut material, Fiber Metal Laminates (FML) often pose challenges during conventional drilling and require judicious selection of machining parameters to ensure defect-free laminates that can serve reliably during their service lifetime. Helical milling is a promising technique for producing good-quality holes [...] Read more.
Being a difficult-to-cut material, Fiber Metal Laminates (FML) often pose challenges during conventional drilling and require judicious selection of machining parameters to ensure defect-free laminates that can serve reliably during their service lifetime. Helical milling is a promising technique for producing good-quality holes and is preferred over conventional drilling. The paper compares conventional drilling with the helical milling technique for producing holes in carbon fiber-reinforced aluminum laminates. The effect of machining parameters, such as cutting speed and axial feed, on the magnitude of cutting force and the machining temperature during conventional drilling as well as helical milling is studied. It was observed that the thrust force produced during machining reduces considerably during helical milling in comparison to conventional drilling at a constant axial feed rate. The highest machining temperature recorded for helical milling was much lower in comparison to the highest machining temperature measured during conventional drilling. The machining temperatures recorded during helical milling were well below the glass transition temperature of the epoxy used in carbon fiber prepreg, hence protecting the prepreg from thermal degradation during the hole-making process. The surface roughness of the holes produced by both techniques is measured, and the surface morphology of the drilled holes is analyzed using a scanning electron microscope. The surface roughness of the helical-milled holes was lower than that for holes produced by conventional drilling. Scanning electron microscope images provided insights into the interaction of the hole surface with the chips during the chip evacuation stage under different speeds and feed rates. The microhardness of the aluminum layers increased after processing holes using drilling and helical milling operations. The axial feed/axial pitch had minimal influence on the microhardness increase in comparison to the cutting speed. Full article
(This article belongs to the Topic Advanced Composites Manufacturing and Plastics Processing)
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9 pages, 1351 KiB  
Communication
Galactic Cosmic Ray Particle Exposure Does Not Increase Protein Levels of Inflammation or Oxidative Stress Markers in Rat Microglial Cells In Vitro
by Danielle S. Cahoon, Derek R. Fisher, Bernard M. Rabin, Stefania Lamon-Fava, Dayong Wu, Tong Zheng and Barbara Shukitt-Hale
Int. J. Mol. Sci. 2024, 25(11), 5923; https://doi.org/10.3390/ijms25115923 (registering DOI) - 29 May 2024
Abstract
Astronauts on exploratory missions will be exposed to galactic cosmic rays (GCR), which can induce neuroinflammation and oxidative stress (OS) and may increase the risk of neurodegenerative disease. As key regulators of inflammation and OS in the CNS, microglial cells may be involved [...] Read more.
Astronauts on exploratory missions will be exposed to galactic cosmic rays (GCR), which can induce neuroinflammation and oxidative stress (OS) and may increase the risk of neurodegenerative disease. As key regulators of inflammation and OS in the CNS, microglial cells may be involved in GCR-induced deficits, and therefore could be a target for neuroprotection. This study assessed the effects of exposure to helium (4He) and iron (56Fe) particles on inflammation and OS in microglia in vitro, to establish a model for testing countermeasure efficacy. Rat microglia were exposed to a single dose of 20 cGy (300 MeV/n) 4He or 2 Gy 56Fe (600 MeV/n), while the control cells were not exposed (0 cGy). Immediately following irradiation, fresh media was applied to the cells, and biomarkers of inflammation (cyclooxygenase-2 [COX-2], nitric oxide synthase [iNOS], phosphorylated IκB-α [pIκB-α], tumor necrosis factor-α [TNFα], and nitrite [NO2]) and OS (NADPH oxidase [NOX2]) were assessed 24 h later using standard immunochemical techniques. Results showed that radiation did not increase levels of NO2 or protein levels of COX-2, iNOS, pIκB-α, TNFα, or NOX2 compared to non-irradiated control conditions in microglial cells (p > 0.05). Therefore, microglia in isolation may not be the primary cause of neuroinflammation and OS following exposures to helium or iron GCR particles. Full article
(This article belongs to the Special Issue The Role of Microglia in Neurological Disorders)
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16 pages, 470 KiB  
Article
Catholic Parishes and Immigrants in Italy: Insights from the Congregations Study in Three Italian Cities
by Marco Guglielmi, Olga Breskaya and Stefano Sbalchiero
Societies 2024, 14(6), 77; https://doi.org/10.3390/soc14060077 (registering DOI) - 29 May 2024
Abstract
As shown by sociological studies, the Catholic Church in Italy is very active in conducting advocacy and providing political representation to immigrants. It is also highly effective in carrying out services for immigrants, as well as fairly receptive to sharing places of worship [...] Read more.
As shown by sociological studies, the Catholic Church in Italy is very active in conducting advocacy and providing political representation to immigrants. It is also highly effective in carrying out services for immigrants, as well as fairly receptive to sharing places of worship with them. However, these sociological observations have been mainly conducted at the national level rather than by exploring the life of parishes through an empirical lens. This article aims, by applying the congregations study methodology, to fill this gap by detecting faith communities as the basic social units of religious life at the city/country level. In doing that, we discuss quantitative data collected in 377 Catholic parishes in the cities of Bologna, Milan, and Brescia. The findings suggest that Catholic parishes: (i) illustrate a low proportion of immigrants in their communities; (ii) show high activity in providing services for immigrants; (iii) are not politically engaged in advocacy for foreign persons at the local level; and (iv) similarly position themselves as politically conservative and liberal while expressing commitments to immigrants. This study confirms the sociological argument regarding the solidarity approach of the Catholic Church in Italy toward immigrants, while highlighting some ambivalent aspects related to cultural diversity and grass-roots political engagement within parishes’ life. Full article
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21 pages, 858 KiB  
Article
Does E-Commerce Construction Boost Farmers’ Incomes? Evidence from China
by Yilan Ye, Jiabin Fang and Jinsong Ye
Sustainability 2024, 16(11), 4595; https://doi.org/10.3390/su16114595 (registering DOI) - 29 May 2024
Abstract
Elevating farmers’ incomes is crucial for ensuring socio-economic stability, yet the current stagnation in income growth and the expanding divide between urban and rural incomes present formidable challenges. E-commerce offers a transformative solution by bridging these disparities and fostering sustainable growth. Utilizing county-level [...] Read more.
Elevating farmers’ incomes is crucial for ensuring socio-economic stability, yet the current stagnation in income growth and the expanding divide between urban and rural incomes present formidable challenges. E-commerce offers a transformative solution by bridging these disparities and fostering sustainable growth. Utilizing county-level data from 2000 to 2020 in China, particularly focusing on “The Pilot Counties of Introducing E-commerce to Rural Areas”, this paper explores the impact of e-commerce construction on farmers’ income growth using the multiple difference method. The findings reveal that e-commerce development exerts a significant positive impact on the enhancement of farmers’ incomes. Mechanistic analyses indicate that the driving effect of the comprehensive demonstration county policy of e-commerce in rural areas on farmers’ incomes is mainly realized through the channels of expanding market accessibility, promoting the advanced transformation of industrial structure, and optimizing the conditions of information infrastructure. Moreover, the efficacy of e-commerce policies in income augmentation is more pronounced in regions characterized by higher levels of human capital and substantial scale effects. This research offers valuable insights for continuously and effectively implementing the “Comprehensive Demonstration of E-commerce in Rural Areas” policy, which are crucial for exploring ways and mechanisms to boost farmers’ incomes in developing countries. Full article
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24 pages, 11762 KiB  
Article
Gut and Urinary Microbiota in Cats with Kidney Stones
by Patrick Joubran, Françoise A. Roux, Matteo Serino and Jack-Yves Deschamps
Microorganisms 2024, 12(6), 1098; https://doi.org/10.3390/microorganisms12061098 (registering DOI) - 29 May 2024
Abstract
Upper urinary tract urolithiasis is an emerging disease in cats, with 98% of kidney stones composed of calcium oxalate. In humans, disturbances in the intestinal and urinary microbiota are suspected to contribute to the formation of calcium oxalate stones. We hypothesized that similar [...] Read more.
Upper urinary tract urolithiasis is an emerging disease in cats, with 98% of kidney stones composed of calcium oxalate. In humans, disturbances in the intestinal and urinary microbiota are suspected to contribute to the formation of calcium oxalate stones. We hypothesized that similar mechanisms may be at play in cats. This study examines the intestinal and urinary microbiota of nine cats with kidney stones compared to nine healthy cats before, during, and after treatment with the antibiotic cefovecin, a cephalosporin. Initially, cats with kidney stones displayed a less diverse intestinal microbiota. Antibiotic treatment reduced microbiota diversity in both groups. The absence of specific intestinal bacteria could lead to a loss of the functions these bacteria perform, such as oxalate degradation, which may contribute to the formation of calcium oxalate stones. This study confirms the presence of a distinct urobiome in cats with kidney stones, characterized by greater richness and diversity compared to healthy cats. These findings highlight the potential of microbiota modulation as a strategy to prevent renal lithiasis in cats. Full article
(This article belongs to the Section Veterinary Microbiology)
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17 pages, 861 KiB  
Article
Changes in the Composition of Olive Pomace after Fermentation: A Preliminary Study
by Diana Melo Ferreira, Susana Machado, Liliana Espírito Santo, Anabela S. G. Costa, Floricuța Ranga, Maria Simona Chiș, Josman D. Palmeira, Maria Beatriz P. P. Oliveira, Rita C. Alves and Helena Ferreira
Fermentation 2024, 10(6), 287; https://doi.org/10.3390/fermentation10060287 (registering DOI) - 29 May 2024
Abstract
Because olive pomace (the main by-product of olive oil production) is phytotoxic, new applications must be investigated to minimize its negative environmental impact. In this work, olive pomace was fermented for 4 and 32 days at room temperature, having in view its valorization [...] Read more.
Because olive pomace (the main by-product of olive oil production) is phytotoxic, new applications must be investigated to minimize its negative environmental impact. In this work, olive pomace was fermented for 4 and 32 days at room temperature, having in view its valorization as a novel food, thereby creating opportunities for the food industry and addressing a challenge of the olive oil sector. The chemical and microbiological modifications that occurred along the fermentation were followed. The results showed no significant differences (p > 0.05) in total protein between the control and the fermented samples; however, the latter exhibited higher levels of essential amino acids. The major nonessential and essential amino acids were glutamic acid and leucine in all samples. There was a significant increase in the total fat of the 32-day sample and the main fatty acid was oleic acid in all samples. There were considerable reductions in total vitamin E, phenolics, and antioxidant activity values post-fermentation. Hydroxytyrosol replaced oleacein as the main phenolic in the 32-day sample. A sharp increase in total microorganisms occurred (2.20 × 102 to 3.00 × 104–2.01 × 107 colony forming units/mL) but no pathogenic microorganisms were detected. Overall, olive pomace fermentation creates novel products for the food industry with a balanced nutritional composition. Full article
(This article belongs to the Special Issue Food Wastes: Feedstock for Value-Added Products: 5th Edition)
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24 pages, 1665 KiB  
Article
Advancing Sustainability through Higher Education: Student Teachers Integrate Inner Development Goals (IDG) and Future-Oriented Methodologies
by Birgitta Nordén
Challenges 2024, 15(2), 28; https://doi.org/10.3390/challe15020028 (registering DOI) - 29 May 2024
Abstract
Methodologies for future-oriented research are mutually beneficial in highlighting different methodological perspectives and proposals for extending higher-education didactics toward sustainability. This study explores how different augmented-reality applications can enable new ways of teaching and learning. It systematically investigates how student teachers (n [...] Read more.
Methodologies for future-oriented research are mutually beneficial in highlighting different methodological perspectives and proposals for extending higher-education didactics toward sustainability. This study explores how different augmented-reality applications can enable new ways of teaching and learning. It systematically investigates how student teachers (n = 18) in higher education experienced ongoing realities while designing learning activities for a hybrid conference and interconnecting sustainability knowings via didactic modeling and design thinking. This qualitative study aims to develop a conceptual hybrid framework concerning the implications of student teachers incorporating design thinking and inner transition into their professional work with future-oriented methodologies on didactic modeling for sustainability commitment. With a qualitative approach, data were collected during and after a hackathon-like workshop through student teachers’ reflections, post-workshop surveys, and observation field notes. The thematic analysis shed light on transgressive learning and a transition in sustainability mindset through the activation of inner dimensions. Findings reinforcing sustainability commitment evolved around the following categories: being authentic (intra-personal competence), collaborating co-creatively (interpersonal competence), thinking long-term-oriented (futures-thinking competence on implementing didactics understanding), relating to creative confidence (values-thinking competence as embodied engagement), and acting based on perseverant professional knowledge-driven change (bridging didactics) by connecting theory-loaded empiricism and empirically loaded theory. The results highlight some of the key features of future-oriented methodologies and approaches to future-oriented methodologies, which include collaboration, boundary crossing, and exploration, and show the conditions that can support or hinder methodological development and innovation. Full article
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14 pages, 1875 KiB  
Article
Retrospective Evaluation of Bone Turnover Markers in Serum for the Prediction of Metastases Development in Breast Cancer Patients: A Cohort Study
by Mariz Kasoha, Sebastian Findeklee, Meletios P. Nigdelis, Gilda Schmidt, Erich-Franz Solomayer and Bashar Haj Hamoud
Biomedicines 2024, 12(6), 1201; https://doi.org/10.3390/biomedicines12061201 (registering DOI) - 29 May 2024
Abstract
Background: Serum bone turnover markers might play a role in the prediction of the development of bone metastases in breast cancer (BC) patients. We conducted a retrospective cohort study to address the association of serum bone turnover markers with oncologic outcomes. Methods: We [...] Read more.
Background: Serum bone turnover markers might play a role in the prediction of the development of bone metastases in breast cancer (BC) patients. We conducted a retrospective cohort study to address the association of serum bone turnover markers with oncologic outcomes. Methods: We included 80 women with BC, who were operated on at the Department of Gynecology, Obstetrics and Reproductive Medicine, Homburg/Saar, Germany. Serum samples were obtained prior to surgery and were used for estimation of the concentration of tumor and bone turnover markers using enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA). Results: At baseline, pyridinoline cross-linked carboxy-terminal telopeptide of type-1 collagen (ICTP) concentrations were higher in nodal positive vs. negative tumors (Mann–Whitney test p = 0.04). After a median follow-up of 79.4 months, 17 patients developed metastases, with 9 demonstrating, among other organs, osseous metastases. ICTP demonstrated the best area under the curve in the predection of osseous metastases in our cohort (AUC = 0.740, DeLong Test p = 0.005). Univariable Cox proportional hazard models failed to demonstrate significant associations between serum bone turnover markers and oncologic outcomes (progression-free survival, overall survival). Conclusions: Serum bone turnover markers (e.g., ICTP) were able to predict the development of osseous metastases but were not associated with oncologic outcomes. Further investigation and validation are required for the use of such markers in clinical practice. Full article
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16 pages, 4677 KiB  
Article
Fatigue Damage Assessment of Turbine Runner Blades Considering Sediment Wear
by Haifeng Chen, Jun Pan, Shuo Wang, Jianfeng Ma and Weiliang Zhang
Appl. Sci. 2024, 14(11), 4660; https://doi.org/10.3390/app14114660 (registering DOI) - 29 May 2024
Abstract
The wear phenomenon that occurs on the blades during operation has a significant impact on the fatigue life of the blades. To address the issue of fatigue life assessment for turbine runner blades subjected to increased dynamic stress due to sediment wear, taking [...] Read more.
The wear phenomenon that occurs on the blades during operation has a significant impact on the fatigue life of the blades. To address the issue of fatigue life assessment for turbine runner blades subjected to increased dynamic stress due to sediment wear, taking a specific high-head hydropower unit’s mixed-flow turbine as the research subject, a hydraulic model of the turbine was established. The wear zones of the runner blades are determined based on the distribution of the flow field’s velocity and the sediment volume fraction. According to the wear rate formula for runner blade material, the amount of wear on the blades is determined, and the dynamic stress data for the dangerous areas of the blades under different degrees of wear are calculated using a unidirectional fluid–structure coupling method. The load spectrum of the time–stress history data for the dangerous area at different levels of wear was compiled using the rain-flow counting statistical method. The operating time ratios for the flood season and the non-flood season are combined. Based on the fatigue cumulative damage theory, the total fatigue damage at the maximum stress part of the runner blade was calculated for different stages of wear, providing a reference for the life calculation of mixed-flow hydraulic turbines. Full article
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22 pages, 857 KiB  
Article
Promoting Sustainable Development of Organizations: Performance Pressure, Workplace Fun, and Employee Ambidextrous Innovation
by Chuanhao Fan, Shiying Zheng and Long Chen
Sustainability 2024, 16(11), 4598; https://doi.org/10.3390/su16114598 (registering DOI) - 29 May 2024
Abstract
In the context of an economic downturn, organizations need to continuously improve their performance requirements for employees, and in the macro environment of intensified competition, organizations need to continuously motivate employees to innovate in order to seize development opportunities. Flexibly adjusting to the [...] Read more.
In the context of an economic downturn, organizations need to continuously improve their performance requirements for employees, and in the macro environment of intensified competition, organizations need to continuously motivate employees to innovate in order to seize development opportunities. Flexibly adjusting to the internal pressure of an organization while ensuring organizational vitality can promote employee adaptive growth and achieve sustainable development in the organization. However, the impact of performance pressure on employee ambidextrous innovation is currently unknown. Based on this, this article explores the positive effects of performance pressure in influencing employee ambidextrous innovation through job involvement from the perspective of Affective Events Theory and uses workplace fun as a moderating variable in order to analyze the internal logic of long-term motivation for employee ambidextrous innovation in organizations. This article conducted a survey and analysis of 362 in-service employees in enterprises and institutions and found the following: (1) performance pressure has a significant positive impact on employee ambidextrous innovation; (2) job involvement plays a partial mediating role between performance pressure and exploratory innovation; (3) workplace fun has a moderating effect on the relationship between job involvement and exploitative innovation, and the higher the level of workplace fun, the stronger the positive impact of job involvement on exploitative innovation. Full article
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11 pages, 4954 KiB  
Article
Facile Preparation Method of TiO2/Activated Carbon for Photocatalytic Degradation of Methylene Blue
by Phuoc Huu Le, Tran Thi Thuy Vy, Vo Van Thanh, Duong Hoang Hieu, Quang-Thinh Tran, Ngoc-Van Thi Nguyen, Ngo Ngoc Uyen, Nguyen Thi Thu Tram, Nguyen Chi Toan, Ly Tho Xuan, Le Thi Cam Tuyen, Nguyen Trung Kien, Yu-Min Hu and Sheng-Rui Jian
Micromachines 2024, 15(6), 714; https://doi.org/10.3390/mi15060714 (registering DOI) - 29 May 2024
Abstract
The development of nanocomposite photocatalysts with high photocatalytic activity, cost-effectiveness, a simple preparation process, and scalability for practical applications is of great interest. In this study, nanocomposites of TiO2 Degussa P25 nanoparticles/activated carbon (TiO2/AC) were prepared at various mass ratios [...] Read more.
The development of nanocomposite photocatalysts with high photocatalytic activity, cost-effectiveness, a simple preparation process, and scalability for practical applications is of great interest. In this study, nanocomposites of TiO2 Degussa P25 nanoparticles/activated carbon (TiO2/AC) were prepared at various mass ratios of (4:1), (3:2), (2:3), and (1:4) by a facile process involving manual mechanical pounding, ultrasonic-assisted mixing in an ethanol solution, paper filtration, and mild thermal annealing. The characterization methods included XRD, SEM-EDS, Raman, FTIR, XPS, and UV-Vis spectroscopies. The effects of TiO2/AC mass ratios on the structural, morphological, and photocatalytic properties were systematically studied in comparison with bare TiO2 and bare AC. TiO2 nanoparticles exhibited dominant anatase and minor rutile phases and a crystallite size of approximately 21 nm, while AC had XRD peaks of graphite and carbon and a crystallite size of 49 nm. The composites exhibited tight decoration of TiO2 nanoparticles on micron-/submicron AC particles, and uniform TiO2/AC composites were obtained, as evidenced by the uniform distribution of Ti, O, and C in an EDS mapping. Moreover, Raman spectra show the typical vibration modes of anatase TiO2 (e.g., E1g(1), B1g(1), Eg(3)) and carbon materials with D and G bands. The TiO2/AC with (4:1), (3:2), and (2:3) possessed higher reaction rate constants (k) in photocatalytic degradation of methylene blue (MB) than that of either TiO2 or AC. Among the investigated materials, TiO2/AC = 4:1 achieved the highest photocatalytic activity with a high k of 55.2 × 10−3 min−1 and an MB removal efficiency of 96.6% after 30 min of treatment under UV-Vis irradiation (120 mW/cm2). The enhanced photocatalytic activity for TiO2/AC is due to the synergistic effect of the high adsorption capability of AC and the high photocatalytic activity of TiO2. Furthermore, TiO2/AC promotes the separation of photoexcited electron/hole (e/h+) pairs to reduce their recombination rate and thus enhance photocatalytic activity. The optimal TiO2/AC composite with a mass ratio of 4/1 is suggested for treating industrial or household wastewater with organic pollutants. Full article
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12 pages, 1638 KiB  
Case Report
Oral Isavuconazole Combined with Nebulized Inhalation and Bronchoscopic Administration of Amphotericin B for the Treatment of Pulmonary Mucormycosis: A Case Report and Literature Review
by Xuan Leng, Hui Zhou, Zhiyang Xu and Feng Xu
J. Fungi 2024, 10(6), 388; https://doi.org/10.3390/jof10060388 (registering DOI) - 29 May 2024
Abstract
Pulmonary mucormycosis (PM) is an invasive and potentially fatal fungal infection, with Rhizopus microsporus (R. microsporus) being the most common pathogen. The routine therapy for this infection includes surgery and antifungal agents. However, the therapeutic effects of single agents are unsatisfactory [...] Read more.
Pulmonary mucormycosis (PM) is an invasive and potentially fatal fungal infection, with Rhizopus microsporus (R. microsporus) being the most common pathogen. The routine therapy for this infection includes surgery and antifungal agents. However, the therapeutic effects of single agents are unsatisfactory due to the rapid progression of mucormycosis, while not all patients can tolerate surgery. Innovative treatment methods like combination therapy await validations of their clinical efficacy. We report a case of PM that was diagnosed via metagenomics next-generation sequencing (mNGS) of black drainage fluid from the patient’s lung. The patient eventually recovered and was discharged after a combination therapy of oral isavuconazole, inhaled amphotericin B, and local perfusion of amphotericin B through bronchoscopy, which may be a promising strategy for the treatment of PM, especially for cases where surgery is not possible. A retrospective study of 297 cases in a literature review highlights the different treatment methods used in clinical practice. Full article
(This article belongs to the Special Issue New Strategies to Combat Human Fungal Infections)
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19 pages, 380 KiB  
Review
Vaccinia Virus: Mechanisms Supporting Immune Evasion and Successful Long-Term Protective Immunity
by Joy Hsu, Suyon Kim and Niroshana Anandasabapathy
Viruses 2024, 16(6), 870; https://doi.org/10.3390/v16060870 (registering DOI) - 29 May 2024
Abstract
Vaccinia virus is the most successful vaccine in human history and functions as a protective vaccine against smallpox and monkeypox, highlighting the importance of ongoing research into vaccinia due to its genetic similarity to other emergent poxviruses. Moreover, vaccinia’s ability to accommodate large [...] Read more.
Vaccinia virus is the most successful vaccine in human history and functions as a protective vaccine against smallpox and monkeypox, highlighting the importance of ongoing research into vaccinia due to its genetic similarity to other emergent poxviruses. Moreover, vaccinia’s ability to accommodate large genetic insertions makes it promising for vaccine development and potential therapeutic applications, such as oncolytic agents. Thus, understanding how superior immunity is generated by vaccinia is crucial for designing other effective and safe vaccine strategies. During vaccinia inoculation by scarification, the skin serves as a primary site for the virus–host interaction, with various cell types playing distinct roles. During this process, hematopoietic cells undergo abortive infections, while non-hematopoietic cells support the full viral life cycle. This differential permissiveness to viral replication influences subsequent innate and adaptive immune responses. Dendritic cells (DCs), key immune sentinels in peripheral tissues such as skin, are pivotal in generating T cell memory during vaccinia immunization. DCs residing in the skin capture viral antigens and migrate to the draining lymph nodes (dLN), where they undergo maturation and present processed antigens to T cells. Notably, CD8+ T cells are particularly significant in viral clearance and the establishment of long-term protective immunity. Here, we will discuss vaccinia virus, its continued relevance to public health, and viral strategies permissive to immune escape. We will also discuss key events and populations leading to long-term protective immunity and remaining key gaps. Full article
(This article belongs to the Special Issue Innate and Adaptive Immunity to Cutaneous Virus Infection)
31 pages, 3277 KiB  
Review
Physiology of Vitamin D—Focusing on Disease Prevention
by Sunil J. Wimalawansa
Nutrients 2024, 16(11), 1666; https://doi.org/10.3390/nu16111666 (registering DOI) - 29 May 2024
Abstract
Vitamin D is a crucial micronutrient, critical to human health, and influences many physiological processes. Oral and skin-derived vitamin D is hydroxylated to form calcifediol (25(OH)D) in the liver, then to 1,25(OH)2D (calcitriol) in the kidney. Alongside the parathyroid hormone, calcitriol [...] Read more.
Vitamin D is a crucial micronutrient, critical to human health, and influences many physiological processes. Oral and skin-derived vitamin D is hydroxylated to form calcifediol (25(OH)D) in the liver, then to 1,25(OH)2D (calcitriol) in the kidney. Alongside the parathyroid hormone, calcitriol regulates neuro-musculoskeletal activities by tightly controlling blood-ionized calcium concentrations through intestinal calcium absorption, renal tubular reabsorption, and skeletal mineralization. Beyond its classical roles, evidence underscores the impact of vitamin D on the prevention and reduction of the severity of diverse conditions such as cardiovascular and metabolic diseases, autoimmune disorders, infection, and cancer. Peripheral target cells, like immune cells, obtain vitamin D and 25(OH)D through concentration-dependent diffusion from the circulation. Calcitriol is synthesized intracellularly in these cells from these precursors, which is crucial for their protective physiological actions. Its deficiency exacerbates inflammation, oxidative stress, and increased susceptibility to metabolic disorders and infections; deficiency also causes premature deaths. Thus, maintaining optimal serum levels above 40 ng/mL is vital for health and disease prevention. However, achieving it requires several times more than the government’s recommended vitamin D doses. Despite extensive published research, recommended daily intake and therapeutic serum 25(OH)D concentrations have lagged and are outdated, preventing people from benefiting. Evidence suggests that maintaining the 25(OH)D concentrations above 40 ng/mL with a range of 40–80 ng/mL in the population is optimal for disease prevention and reducing morbidities and mortality without adverse effects. The recommendation for individuals is to maintain serum 25(OH)D concentrations above 50 ng/mL (125 nmol/L) for optimal clinical outcomes. Insights from metabolomics, transcriptomics, and epigenetics offer promise for better clinical outcomes from vitamin D sufficiency. Given its broader positive impact on human health with minimal cost and little adverse effects, proactively integrating vitamin D assessment and supplementation into clinical practice promises significant benefits, including reduced healthcare costs. This review synthesized recent novel findings related to the physiology of vitamin D that have significant implications for disease prevention. Full article
(This article belongs to the Section Nutrition and Public Health)
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11 pages, 8631 KiB  
Case Report
Pathogenesis of Hemophagocytic Lymphohistiocytosis/Macrophage Activation Syndrome: A Case Report and Review of the Literature
by Chiara Gioia, Marino Paroli, Raffaella Izzo, Lorenzo Di Sanzo, Elisabetta Rossi, Pasquale Pignatelli and Daniele Accapezzato
Int. J. Mol. Sci. 2024, 25(11), 5921; https://doi.org/10.3390/ijms25115921 (registering DOI) - 29 May 2024
Abstract
Hemophagocytic lymphohistiocytosis (HLH) is a life-threatening condition characterized by the uncontrolled activation of cytotoxic T lymphocytes, NK cells, and macrophages, resulting in an overproduction of pro-inflammatory cytokines. A primary and a secondary form are distinguished depending on whether or not it is associated [...] Read more.
Hemophagocytic lymphohistiocytosis (HLH) is a life-threatening condition characterized by the uncontrolled activation of cytotoxic T lymphocytes, NK cells, and macrophages, resulting in an overproduction of pro-inflammatory cytokines. A primary and a secondary form are distinguished depending on whether or not it is associated with hematologic, infectious, or immune-mediated disease. Clinical manifestations include fever, splenomegaly, neurological changes, coagulopathy, hepatic dysfunction, cytopenia, hypertriglyceridemia, hyperferritinemia, and hemophagocytosis. In adults, therapy, although aggressive, is often unsuccessful. We report the case of a 41-year-old man with no apparent history of previous disease and an acute onset characterized by fever, fatigue, and weight loss. The man was from Burkina Faso and had made trips to his home country in the previous five months. On admission, leukopenia, thrombocytopenia, increased creatinine and transaminases, LDH, and CRP with a normal ESR were found. The patient also presented with hypertriglyceridemia and hyperferritinemia. An infectious or autoimmune etiology was ruled out. A total body CT scan showed bilateral pleural effusion and hilar mesenterial, abdominal, and paratracheal lymphadenopathy. Lymphoproliferative disease with HLH complication was therefore suspected. High doses of glucocorticoids were then administered. A cytologic analysis of the pleural effusion showed anaplastic lymphoma cells and bone marrow aspirate showed hemophagocytosis. An Epstein–Barr Virus (EBV) DNA load of more than 90000 copies/mL was found. Bone marrow biopsy showed a marrow localization of peripheral T lymphoma. The course was rapidly progressive until the patient died. HLH is a rare but usually fatal complication in adults of hematologic, autoimmune, and malignant diseases. Very early diagnosis and treatment are critical but not always sufficient to save patients. Full article
(This article belongs to the Special Issue Advanced Research on Immune Cells and Cytokines)
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20 pages, 16966 KiB  
Article
Path Tracking Control Based on T-S Fuzzy Model for Autonomous Vehicles with Yaw Angle and Heading Angle
by Yelin He, Jian Wu, Fuxing Xu, Xin Liu, Shuai Wang and Guanjie Cui
Machines 2024, 12(6), 375; https://doi.org/10.3390/machines12060375 (registering DOI) - 29 May 2024
Abstract
Existing vehicle-road models used for road tracking do not take into account the side slip angle, which leads to a reduction in road tracking accuracy in scenarios where the vehicle is at a large side slip angle, such as an emergency lane change. [...] Read more.
Existing vehicle-road models used for road tracking do not take into account the side slip angle, which leads to a reduction in road tracking accuracy in scenarios where the vehicle is at a large side slip angle, such as an emergency lane change. Consequently, this study presents a path-tracking control technique based on the T-S fuzzy model of heading angle vehicle autonomy. In this paper, based on the yaw angle-based vehicle tracking model, a heading angle-based tracking model considering the side slip angle is constructed. Second, since the vehicle speed varies with time, this paper selects the membership function of the vehicle speed to establish the T-S fuzzy model of autonomous vehicle based on the yaw angle and heading angle, respectively, and ensures the robustness and stability over the whole parameter space by the linear parameter variation robust H controller. Then, cost functions based on the yaw angle and heading angle augmented error systems are created separately to optimize the system’s overall performance. Ultimately, simulation and experimentation confirm that the algorithm for control, which is based on the fuzzy model of the heading angle vehicle, has superior autonomous trajectory performance. Full article
(This article belongs to the Special Issue New Trends in Robotics and Automation)
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8 pages, 325 KiB  
Article
Impact of Exogenous Factors and Anesthetic Risk in Premature Birth during the Pandemic Period
by Florin Tovirnac, Carolina Susanu, Nicoleta Andreea Tovirnac, Eva Maria Elkan, Ana Maria Cobzaru, Alexandru Nechifor and Alina Mihaela Calin
Diagnostics 2024, 14(11), 1123; https://doi.org/10.3390/diagnostics14111123 (registering DOI) - 29 May 2024
Abstract
Background: Premature birth remains a public health problem worldwide, involving a broader context and a multidisciplinary team aimed at combating this phenomenon as much as possible. The consumption of addictive substances by women who are pregnant can occur in different social contexts and [...] Read more.
Background: Premature birth remains a public health problem worldwide, involving a broader context and a multidisciplinary team aimed at combating this phenomenon as much as possible. The consumption of addictive substances by women who are pregnant can occur in different social contexts and at different stages of their lives, which modulate its extent. Obstetricians and anesthetists should consider the anesthetic maternal risks that may arise due to these addictive behaviors. The maternal anesthetic risk is higher in women who are pregnant with a medium-level of education, imbalanced nutrition, stress associated with physical or mental activity, affected sleep hygiene, and failed marriages. Objectives: The objectives of the study refer to analyzing the impact of exogenous factors and the anesthetic risk on premature birth for women who were pregnant during the pandemic period and in women who were pregnant without COVID-19 infection. The authors studied a significant sample of 3588 women who were pregnant without COVID-19 infection, among whom 3291 gave birth at term and 297 gave birth prematurely. Methods: The methods analyzed consist of studying the specialized literature regarding the impact of exogenous factors and parturient’s anesthetic risk on premature birth and identifying the regional risk profile of women who are pregnant in the southeast region of Romania compared to that identified in the specialized literature. In the analytical methods, we used a linear regression to study the incidence of exogenous risk factors on anesthetic risk in women who were pregnant with premature births compared to those with full-term births. Results: The results confirm the significant impact of exogenous factors on anesthetic risk and the significant impact of anesthetic risk on premature births. The novelty of the study lies in highlighting the modification of the regional exogenous risk profile during the pandemic period in southeast Romania due to unfavorable socio-economic causes and the translation of grade I and II prematurity events to higher frequencies with an increased level of maternal anesthetic risk. Conclusions: The study findings show that the anesthetic risk is maximized in parturients with a middle school education. Additionally, the anesthetic risk of patients who are pregnant increases with the intensification of smoking adherence and its maintenance throughout the pregnancy at the same intensity. Our study aims to provide a basis for the diversification and development of community intervention programs in the post-COVID-19 era, considering the reshaping of social models and the repositioning of social principles and values. Obstetricians and anesthetists must know and promote family values to harmonize the lives of family members and provide a better life for the mother and child. Full article
(This article belongs to the Section Pathology and Molecular Diagnostics)
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21 pages, 9980 KiB  
Case Report
The Study of Groundwater in the Zhambyl Region, Southern Kazakhstan, to Improve Sustainability
by Dinara Adenova, Dani Sarsekova, Malis Absametov, Yermek Murtazin, Janay Sagin, Ludmila Trushel and Oxana Miroshnichenko
Sustainability 2024, 16(11), 4597; https://doi.org/10.3390/su16114597 (registering DOI) - 29 May 2024
Abstract
Water resources are scarce and difficult to manage in Kazakhstan, Central Asia (CA). Anthropic activities largely eliminated the Aral Sea. Afghanistan’s large-scale canal construction may eliminate life in the main stream of the Amu Darya River, CA. Kazakhstan’s HYRASIA ONE project, with a [...] Read more.
Water resources are scarce and difficult to manage in Kazakhstan, Central Asia (CA). Anthropic activities largely eliminated the Aral Sea. Afghanistan’s large-scale canal construction may eliminate life in the main stream of the Amu Darya River, CA. Kazakhstan’s HYRASIA ONE project, with a EUR 50 billion investment to produce green hydrogen, is targeted to withdraw water from the Caspian Sea. Kazakhstan, CA, requires sustainable programs that integrate both decision-makers’ and people’s behavior. For this paper, the authors investigated groundwater resources for sustainable use, including for consumption, and the potential for natural “white” hydrogen production from underground geological “factories”. Kazakhstan is rich in natural resources, such as iron-rich rocks, minerals, and uranium, which are necessary for serpentinization reactions and radiolysis decay in natural hydrogen production from underground water. Investigations of underground geological “factories” require substantial efforts in field data collection. A chemical analysis of 40 groundwater samples from the 97 wells surveyed and investigated in the T. Ryskulov, Zhambyl, Baizak and Zhualy districts of the Zhambyl region in South Kazakhstan in 2021–2022 was carried out. These samples were compared with previously collected water samples from the years 2020–2021. The compositions of groundwater samples were analyzed, revealing various concentrations of different minerals, natural geological rocks, and anthropogenic materials. South Kazakhstan is rich in natural mineral resources. As a result, mining companies extract resources in the Taraz–Zhanatas–Karatau and the Shu–Novotroitsk industrial areas. The most significant levels of minerals found in water samples were found in the territory of the Talas–Assinsky interfluve, where the main industrial mining enterprises are concentrated and the largest groundwater deposits have been explored. Groundwater compositions have direct connections to geological rocks. The geological rocks are confined to sandstones, siltstones, porphyrites, conglomerates, limestones, and metamorphic rocks. In observation wells, a number of components can be found in high concentrations (mg/L): sulfates—602.0 (MPC 500 mg/L); sodium—436.5 (MPC 200 mg/L); chlorine—465.4 (MPC 350 mg/L); lithium—0.18 (MPC 0.03 mg/L); boron—0.74 (MPC 0.5 mg/L); cadmium—0.002 (MPC 0.001 mg/L); strontium—15, 0 (MPC 7.0 mg/L); and TDS—1970 (MPC 1000). The high mineral contents in the water are natural and comprise minerals from geological sources, including iron-rich rocks, to uranium. Proper groundwater classifications for research investigations are required to separate potable groundwater resources, wells, and areas where underground geological “factories” producing natural “white” hydrogen could potentially be located. Our preliminary investigation results are presented with the aim of creating a large-scale targeted program to improve water sustainability in Kazakhstan, CA. Full article
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18 pages, 2527 KiB  
Article
Comparative Analysis of Lithium-Ion Batteries for Urban Electric/Hybrid Electric Vehicles
by Boris Velev, Bozhidar Djudzhev, Vladimir Dimitrov and Nikolay Hinov
Batteries 2024, 10(6), 186; https://doi.org/10.3390/batteries10060186 (registering DOI) - 29 May 2024
Abstract
This paper presents an experimental comparison of two types of Li-ion battery stacks for low-voltage energy storage in small urban Electric or Hybrid Electric Vehicles (EVs/HEVs). These systems are a combination of lithium battery cells, a battery management system (BMS), and a central [...] Read more.
This paper presents an experimental comparison of two types of Li-ion battery stacks for low-voltage energy storage in small urban Electric or Hybrid Electric Vehicles (EVs/HEVs). These systems are a combination of lithium battery cells, a battery management system (BMS), and a central control circuit—a lithium energy storage and management system (LESMS). Li-Ion cells are assembled with two different active cathode materials, nickel–cobalt–aluminum (NCA) and lithium iron phosphate (LFP), both with an integrated decentralized BMS. Based on experiments conducted on the two assembled LESMSs, this paper suggests that although LFP batteries have inferior characteristics in terms of energy and power density, they have great capacity for improvement. Full article
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12 pages, 1940 KiB  
Article
Systematics Review and Phylogeny of Cyrtophyllitinae Zeuner, 1935 sensu Gorochov, Jarzembowski & Coram, 2006 (Ensifera, Haglidae), with Description of Two New Species
by Jun-Jie Gu, Wei Yuan, Rong Huang, Dong Ren and Hong-Xing Chen
Insects 2024, 15(6), 396; https://doi.org/10.3390/insects15060396 (registering DOI) - 29 May 2024
Abstract
A phylogeny of Cyrtophyllitinae Zeuner, 1935 sensu Gorochov, Jarzembowski & Coram, 2006, based on wing morphology, is presented including all genera. Cyrtophillitinae is found to be paraphyletic. Except for Cyrtophyllites rogeri Oppenheim, 1888, all other species were moved from the subfamily Cyrtophyllitinae (Hagloidea, [...] Read more.
A phylogeny of Cyrtophyllitinae Zeuner, 1935 sensu Gorochov, Jarzembowski & Coram, 2006, based on wing morphology, is presented including all genera. Cyrtophillitinae is found to be paraphyletic. Except for Cyrtophyllites rogeri Oppenheim, 1888, all other species were moved from the subfamily Cyrtophyllitinae (Hagloidea, Haglidae). Consequently, a new subfamily Archaboilinae subfam. nov. was erected and accommodates most of the previous cyrtophillitine taxa, except Cyrtophyllites rogeri. The type genus Archaboilus Martynov, 1937 of the new subfamily was designated; a new genus, Pararchaboilus gen. nov., was erected with the designation of type species Pararchaboilus cretaceus comb. nov. From the Middle Jurassic deposits of China, two new species, Archaboilus ornatus sp. nov. and Vitimoilus gigantus sp. nov., are described. Full article
(This article belongs to the Section Insect Systematics, Phylogeny and Evolution)
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23 pages, 8980 KiB  
Article
Numerical Study of the Force Characteristics and Flow Field Patterns of a Cylinder in the Internal Solitary Wave
by Miao Zhang, Haibao Hu, Abdellatif Ouahsine, Peng Du, Xiao Huang and Luo Xie
J. Mar. Sci. Eng. 2024, 12(6), 906; https://doi.org/10.3390/jmse12060906 (registering DOI) - 29 May 2024
Abstract
The density of the ocean is unevenly distributed along the depth direction, showing a stratified structure. When there is an external disturbance, large-scale internal solitary waves are easily generated. The internal solitary waves are bounded by the intermediate pycnocline, and the currents in [...] Read more.
The density of the ocean is unevenly distributed along the depth direction, showing a stratified structure. When there is an external disturbance, large-scale internal solitary waves are easily generated. The internal solitary waves are bounded by the intermediate pycnocline, and the currents in the upper and lower layers will flow in opposite directions. This generates strong shear forces that threaten the safety of marine structures. In this paper, the flow field distribution characteristics of a cylinder under the action of internal solitary waves at different scales are analyzed as a research object. The whole cylinder is discretized into 40 regions, and the horizontal force applied to each section of the cylinder is extracted. The force characteristics of the cylinder are analyzed. It is concluded that the pressure is the main factor determining the magnitude of the total combined force. In addition, the paper extracts the main flow structures from the modal decomposition point of view and explains the reasons affecting the force behavior of the cylinder. Full article
(This article belongs to the Special Issue Application of CFD Simulations to Marine Hydrodynamic Problems)
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