Humping is a common defect in direct energy deposition processes that reduces the geometric integrity of printed products. The available literature on humping detection is deemed reactive, as they focus on detecting late-stage melt pool spatial abnormalities. Therefore, this work introduces a novel, proactive indicator designed to detect early-stage spatiotemporal abnormalities. Specifically, the proposed indicator monitors the variability of instantaneous melt pool solidification-front speed (VIMPS). The solidification front dynamics quantify the intensity of cyclic melt pool elongation induced by early-stage humping. VIMPS tracks the solidification front dynamics based on the variance in the melt pool infrared radiations. Qualitative and quantitive analysis of the collected infrared data confirms VIMPS’s utility in reflecting the intricate humping-induced dynamics and defects. Experimental results proved VIMPS’ proactivity. By capturing early spatiotemporal abnormalities, VIMPS predicted humping by up to 10 s before any significant geometric defects. In contrast, current spatial abnormality-based methods failed to detect humping until 20 s after significant geometric defects had occurred. VIMPS’ proactive detection capabilities enable effective direct energy deposition control, boosting the process’s productivity and quality. Full article

Lakes are threatened by contemporary climate change and human activities. Paleohydrological records provide important evidence for developing scenarios for future changes in the availability of freshwater resources. This study presents a synthesis of a sedimentological, archeological, and chronological dataset collected from Lake Annecy (eastern France) to reconstruct a lake-level record documenting the whole Holocene. This dataset shows a pronounced minimum in the lake level during the Holocene thermal maximum (HTM) (ca. 9000–7000 cal BP), preceded by a general lowering trend (early Holocene), and followed by a general rising trend (Neoglacial). On both the millennial and centennial scales, the Lake Annecy record appears to match the regional pattern of Holocene lake-level fluctuations established for West-Central Europe. In agreement with other extra-regional paleoclimatic records, it shows the dominant influence of orbital forcing. The high magnitude of the lake-level lowering (more than 5 m) during the HTM, with a 2–2.5 °C difference between the HTM and the pre-industrial mean summer temperatures, suggests possible drastic lake-level lowering phases in the near future depending on the IPCC scenarios following climate change. This would mean dramatic impacts on human activities and the preservation of exceptional archeological remains in regional lake basins. Full article

This paper introduces and explores the dynamics of a novel three-dimensional (3D) fractional map with hidden dynamics. The map is constructed through the integration of a discrete sinusoidal memristive into a discrete Duffing map. Moreover, a mathematical operator, namely, a fractional variable-order Caputo-like difference operator, is employed to establish the fractional form of the map with short memory. The numerical simulation results highlight its excellent dynamical behavior, revealing that the addition of the piecewise fractional order makes the memristive-based Duffing map even more chaotic. It is characterized by distinct features, including the absence of an equilibrium point and the presence of multiple hidden chaotic attractors. Full article

The observation of the phytoplankton distribution with a high spatiotemporal resolution is necessary to track the nutrient sources that cause algal blooms and to understand their behavior in response to hydraulic phenomena. Photography from UAVs, which has an excellent temporal and spatial resolution, is an effective method to obtain water quality information comprehensively. In this study, we attempted to develop a method for estimating the chlorophyll concentration from aerial images using machine learning that considers brightness correction based on insolation and the spatial distribution of turbidity evaluated by satellite image analysis. The reflectance of harmful algae bloom (HAB) was different from that of phytoplankton seen under normal conditions; so, the images containing HAB were the causes of error in the estimation of the chlorophyll concentration. First, the images when the bloom occurred were extracted by the discrimination with machine learning. Then, the other images were used for the regression of the concentration. Finally, the coefficient of determination between the estimated chlorophyll concentration when no bloom occurred by the image analysis and the observed value reached 0.84. The proposed method enables the detailed depiction of the spatial distribution of the chlorophyll concentration, which contributes to the improvement in water quality management in reservoirs. Full article

Since the publication of the seminal work People and Pixels: Linking Remote Sensing and the Social Sciences, the call to “socialize the pixel” and “pixelize the social” has gone largely unheeded from a truly participatory research context. Instead, participatory remote sensing has primarily involved ground truthing to verify remote sensing observations and/or participatory mapping methods to complement remotely sensed data products. However, the recent proliferation of relatively low-cost, ready-to-fly small unoccupied aerial systems (sUAS), colloquially known as drones, may be changing this trajectory. sUAS may provide a means for community participation in all aspects of the photogrammetric/remote sensing process, from mission planning and data acquisition to data processing and analysis. We present an overview of the present state of so-called participatory sUAS through a comprehensive literature review of recent English-language journal articles. This is followed by an overview of our own experiences with the use of sUAS in a multi-year participatory research project in an agroecological system encompassing a tri-county/tri-state region in the Southern Great Plains, USA. We conclude with a discussion of opportunities and challenges associated with our experience. Full article

This manuscript explores the interdisciplinary integration of quantum dot–hydrogel composites and smart materials and their applications across a spectrum of fields, including biomedical engineering, environmental sensing, and energy harvesting. It covers the synthesis of novel materials like fluorescent hydrogel nanocomposites that display enhanced chemical stability, mechanical strength, and thermal resistance, highlighting their utility in environmental monitoring and catalysis. In the biomedical sector, innovations include hydrogel composites for targeted drug delivery and advanced therapies such as photothermal DNA hydrogels for tumor treatment. This review also discusses the application of these materials in imaging, diagnostics, and the development of smart sensors capable of detecting various biological and environmental changes. Its scope further extends to optoelectronics and the design of energy-efficient systems, underscoring the versatile functionalities of hydrogels in modern technological applications. Challenges remain in scaling up these technologies for commercial use and ensuring their long-term stability and safety, necessitating future research focused on sustainable, scalable solutions that can be integrated into existing systems. Full article

Diverse larval habitats significantly influence female mosquito oviposition. Utilizing traps that simulate these habitats is helpful in the study of the bioecology and characteristics of pathogen-transmitting species during oviposition. This study evaluated the feasibility of different traps in natural environments by comparing sampling methods and detecting the oviposition of epidemiologically important mosquitoes, with emphasis on Haemagogus species, in a fragment of the Atlantic Forest in Silva Jardim, Rio de Janeiro State, Brazil. Monthly collections were conducted from March 2021 to October 2023 using four types of traps: plastic containers, tires, bamboo, and sapucaia. Immatures were collected from these traps using a pipette, placed in plastic bags, and transported to the laboratory. Tire was the most efficient trap, showing the highest mosquito abundance (n = 1239) and number of species (S = 11). Conversely, the plastic container trap exhibited the lowest diversity (H = 0.43), with only two species and a low mosquito abundance (n = 26). The bamboo trap captured six species and recorded the second-highest diversity index (H = 1.04), while the sapucaia trap captured five species and had the third-highest diversity index (H = 0.91). Of the total immatures collected, 1817 reached adulthood, comprising 13 species, two of which are vectors of the sylvatic yellow fever virus: Haemagogus leucocelaenus and Haemagogus janthinomys. In conclusion, detecting key vectors of the sylvatic yellow fever virus in Brazil highlights the need for ongoing entomological and epidemiological surveillance in the study area and its vicinity. These efforts are crucial for monitoring vector presence and activity, identifying potential transmission hotspots, and devising effective control and prevention strategies. Full article

The complex structure, chemical composition, and biomechanical properties of craniofacial cartilaginous structures make them challenging to reconstruct. Autologous grafts have limited tissue availability and can cause significant donor-site morbidity, homologous grafts often require immunosuppression, and alloplastic grafts may have high rates of infection or displacement. Furthermore, all these grafting techniques require a high level of surgical skill to ensure that the reconstruction matches the original structure. Current research indicates that additive manufacturing shows promise in overcoming these limitations. Autologous stem cells have been developed into cartilage when exposed to the appropriate growth factors and culture conditions, such as mechanical stress and oxygen deprivation. Additive manufacturing allows for increased precision when engineering scaffolds for stem cell cultures. Fine control over the porosity and structure of a material ensures adequate cell adhesion and fit between the graft and the defect. Several recent tissue engineering studies have focused on the trachea, nose, and ear, as these structures are often damaged by congenital conditions, trauma, and malignancy. This article reviews the limitations of current reconstructive techniques and the new developments in additive manufacturing for tracheal, nasal, and auricular cartilages. Full article

This work introduces a neuromorphic sensor (NS) based on force-sensing resistors (FSR) and spiking neurons for robotic systems. The proposed sensor integrates the FSR in the schematic of the spiking neuron in order to make the sensor generate spikes with a frequency that depends on the applied force. The performance of the proposed sensor is evaluated in the control of a SMA-actuated robotic finger by monitoring the force during a steady state when the finger pushes on a tweezer. For comparison purposes, we performed a similar evaluation when the SNN received input from a widely used compression load cell (CLC). The results show that the proposed FSR-based neuromorphic sensor has very good sensitivity to low forces and the function between the spiking rate and the applied force is continuous, with good variation range. However, when compared to the CLC, the response of the NS follows a logarithmic-like function with improved sensitivity for small forces. In addition, the power consumption of NS is 128 µW that is 270 times lower than that of the CLC which needs 3.5 mW to operate. These characteristics make the neuromorphic sensor with FSR suitable for bioinspired control of humanoid robotics, representing a low-power and low-cost alternative to the widely used sensors. Full article

Woodpeckers exhibit selectivity when choosing tree cavities for nest development in forest ecosystems, and fungi play a significant and important role in this ecological process. Therefore, there is a complex and intricate relationship between the various behaviors of woodpeckers and the occurrence of fungal species. Research into the complex bond between fungi and woodpeckers was undertaken to provide more information about this remarkable ecological relationship. Through the process of line transect sampling, woodpecker traces were searched for, and mist nets were set up to capture them. A total of 21 woodpeckers belonging to four species were captured. High-throughput sequencing of the ITS region was performed on fungal-conserved samples to enable an in-depth analysis of the fungal communities linked to the woodpeckers’ nests. Members of Ascomycota were the most abundant in the samples, accounting for 91.96% of the total, demonstrating the importance of this group in the forest ecosystem of this station. The statistical results indicate significant differences in the fungal diversity carried by woodpeckers among the different groups. Species of Cladosporium were found to be the most prevalent of all the detected fungal genera, accounting for 49.3%. The top 15 most abundant genera were Cladosporium, Trichoderma, Beauveria, Epicococcum, Hypoxylon, Penicillium, Nigrospora, Aspergillus, Oidiodendron, Cercospora, Talaromyces, Phialemo-nium, Petriella, Cordyceps, and Sistotrema. The standard Bray–Curtis statistical technique was used in a hierarchical clustering analysis to compute inter-sample distances, allowing for the identification of patterns and correlations within the dataset. We discovered that in the grouped samples from woodpeckers, there were differences in the diversity of fungal communities carried by four woodpecker species, but the less dominant fungal species were still similar. The findings highlight the need to consider these diverse ecological linkages in woodpecker research and conservation efforts. Full article

This study explores how financial development and openness influence the effectiveness of fiscal and monetary policies. An analysis of data from about 100 countries between 1980 and 2018 reveals that both financial openness and development weaken the impact of monetary and fiscal policies. Our results further show that financial development in a country diminishes policy effectiveness depending on the country’s level of financial development; specifically, the more developed a country, the less effective the policies would be. Additionally, through a detailed examination employing a dynamic panel GMM approach, the study investigates the global repercussions of economic downturns in the US and how financial maturity shapes policy effectiveness during these times. We also discuss some policy implications that show that the positive impacts of monetary policy on output growth are lessened during crisis periods, and policymakers should act accordingly. Full article

This article explores the significance of Bitcoin halving events within the cryptocurrency ecosystem and their impact on market dynamics. While the existing literature addresses the periods before and after Bitcoin halving, as well as financial bubbles, there is an absence of forecasting regarding Bitcoin price in the time after halving. To address this gap and provide predictions of Bitcoin price development, we conducted a rigorous analysis of past halving events in 2012, 2016, and 2020, focusing on Bitcoin price behaviour before and after each occurrence. What interests us is not only the change in the price level of Bitcoins (top and bottom), but also when this turn occurs. Through synthesizing data and trends from previous events, this article aims to uncover patterns and insights that illuminate the impact of Bitcoin halving on market dynamics and sustainability, movement of the price level, the peaks reached, and price troughs. Our approach involved employing methods such as RSI, MACD, and regression analysis. We looked for the relationship between the price of Bitcoin (top and bottom) and the number of days after the halving. We have uncovered a mathematical model, according to which the next peak will be reached 19 months (in November 2025) and the trough 31 months after Bitcoin halving 2024 (in November 2026). Looking towards the future, this study estimates predictions and expectations for the upcoming Bitcoin halving. These discoveries significantly enhance our understanding of Bitcoin’s trajectory and its implications for the finance cryptocurrency market. By offering novel insights into cryptocurrency market dynamics, this study contributes to advancing knowledge in the field and provides valuable information for cryptocurrency markets, investors, and stakeholders. Full article

This scoping review synthesizes the evidence from eleven key studies to assess the impact of European Structural and Investment Funds (ESIFs) on regional development across the European Union (EU), focusing on fund efficiency, regional disparities and convergence, governance quality, economic freedom, and fund management. A systematic search was conducted across multiple databases to identify the relevant literature published up to 2023. Eleven studies were selected based on the date published and their focus on ESIFs’ role in regional development, employing a range of methodological approaches including Data Envelopment Analysis (DEA), spatial econometrics, and multivariate analyses. The thematic analysis identified four main categories: Methodological Approaches in Evaluating Fund Efficiency, Regional Disparities and Convergence, The Interconnection between Governance Quality, Economic Freedom, and the Efficiency of Structural Fund Management, and The Absorption Capacity and Fund Management. The review highlights the importance of sophisticated analytical tools in evaluating fund efficiency, with DEA and spatial econometrics providing critical insights into fund management efficiency. Studies underscored the nuanced efficacy of ESIFs in reducing regional disparities, albeit pointing to the need for more targeted fund allocation. Governance quality and economic freedom emerged as pivotal factors enhancing fund management efficiency, suggesting the potential of governance reforms in optimizing ESIF allocation and utilization. Challenges related to fund absorption and management were illuminated, advocating for enhanced institutional management capabilities and the development of innovative performance indicators. The findings of this scoping review contribute to a deeper understanding of the complexities surrounding ESIFs’ impact on regional development within the EU. They underscore the critical importance of governance quality, economic freedom, methodological rigor, and strategic fund allocation in enhancing the effectiveness of ESIFs. The review calls for tailored policy interventions and the integration of national and European funding strategies to maximize the impact of these programs on regional development and SME support. Future research should continue to refine these methodological approaches and explore the causal effects of funding, to enhance our understanding of ESIFs’ efficiency in promoting regional development and convergence within the European Union. Full article

Fetal cardiac intervention (FCI) is an emerging and rapidly advancing group of interventions designed to improve outcomes for fetuses with cardiovascular disease. Currently, FCI is comprised of pharmacologic therapies (e.g., trans-placental antiarrhythmics for fetal arrhythmia), open surgical procedures (e.g., surgical resection of pericardial teratoma), and catheter-based procedures (e.g., fetal aortic valvuloplasty for aortic stenosis). This review focuses on the rationale, criteria for inclusion, technical details, and current outcomes of the three most frequently performed catheter-based FCI procedures: (1) aortic valvuloplasty for critical aortic stenosis (AS) associated with evolving hypoplastic left heart syndrome (HLHS), (2) atrial septal intervention for HLHS with severely restrictive or intact atrial septum (R/IAS), and (3) pulmonary valvuloplasty for pulmonary atresia with intact ventricular septum (PA/IVS). Full article

Management of breeding stallions is crucial to equine reproduction. The longevity of the breeding career is the ultimate objective, whether the stallion is used for natural cover or for semen collection and artificial insemination. Stud farm veterinarians should be aware of the techniques used to evaluate testicular function and the diagnostic approach to testicular disorders in cases of emergency. This paper presents the clinical methods used to evaluate testicular health, including palpation, ultrasonography, biopsy, and fine-needle aspiration. The discussion of testicular disorders is broken down into four categories: congenital disorders (cryptorchidism, monorchidism, and testicular hypoplasia), differential diagnosis of scrotal enlargement, differential diagnosis of causes of progressive testicular enlargement, and differential diagnosis of testicular asymmetry or reduction in size with an emphasis on testicular degeneration. The sudden increase in testicular size is often accompanied by severe clinical signs and is a major cause for referral of stallion for surgery. Testicular disorders are illustrated with clinical cases seen by the authors. Full article

Smoking is a pathogenic factor for pulmonary hypertension (PH). Our previous study showed that serum miR-21 levels are elevated in smokers. miR-21 is considered as engaged in the PH process; however, its mechanisms remain unclear. In this investigation, we found that in the lung tissue of smoking-induced PH patients, the levels of miR-21 and aging markers (p21 and p16) were upregulated, and the function of pulmonary vascular endothelial cells was also impaired. Exposure of mice to cigarette smoke (CS) for four months caused similar changes in lung tissues and increased pulmonary arterial pressure, which were attenuated by knockout of miR-21. Further, human umbilical vein endothelial cells (HUVECs) exposed to cigarette smoke extract (CSE) revealed upregulation of miR-21 levels, depression of PTEN, activation of PI3K/AKT/mTOR signaling, an increase in senescence indexes, and enhanced dysfunction. Inhibiting miR-21 overexpression reversed the PTEN-mTOR signaling pathway and prevented senescence and dysfunction of HUVECs. In sum, our data indicate that miR-21-mediated endothelial senescence and dysfunction are involved in CS-induced PH through the activation of PI3K/AKT/mTOR signaling, which suggests that selective miR-21 inhibition offers the potential to attenuate PH. Full article

During the fermentation process of Oolong tea, significant changes occur in both its external characteristics and its internal components. This study aims to determine the fermentation degree of Oolong tea using visible–near–infrared spectroscopy (vis-VIS-NIR) and image processing. The preprocessed vis-VIS-NIR spectral data are fused with image features after sequential projection algorithm (SPA) feature selection. Subsequently, traditional machine learning and deep learning classification models are compared, with the support vector machine (SVM) and convolutional neural network (CNN) models yielding the highest prediction rates among traditional machine learning models and deep learning models with 97.14% and 95.15% in the prediction set, respectively. The results indicate that VIS-NIR combined with image processing possesses the capability for rapid non-destructive online determination of the fermentation degree of Oolong tea. Additionally, the predictive rate of traditional machine learning models exceeds that of deep learning models in this study. This study provides a theoretical basis for the fermentation of Oolong tea. Full article

Kombucha is a two-stage fermented sweetened tea beverage that uses yeast and lactic acid bacteria (LAB) to convert sugars into ethanol and lactate and acetic acid bacteria (AAB) to oxidize ethanol to acetate. Its popularity as a beverage grew from claims of health benefits derived from this vibrant microbial bioconversion. While recent studies have shed light on the diversity of cultures in Kombucha fermentation, there is limited information on the diversity, and especially viability, of cultures in retail beverages that advertise the presence of Kombucha and probiotic cultures. In this study, 12 Kombucha beverages produced by different manufacturers throughout the US were purchased and microbially characterized. Eight of the beverages contained viable Kombucha cultures, while 3 of the remaining 4 had viable Bacillus cultures as added probiotics. Amplicon profiling revealed that all contained Kombucha yeast and bacteria cells. The dominant yeasts detected were Lachancea cidri (10/12), Brettanomyces (9/12), Malassezia (6/12), and Saccharomyces (5/12). Dominant LAB included Liquorilactobacillus and Oenococcus oeni, and AAB were Komagataeibacter, Gluconobacter, and Acetobacter. One beverage had a significant amount of Zymomonas mobilis, an ethanol-producing bacterium from Agave cactus. While Kombucha beverages differ in the types and viability of cultures, all except one beverage contained detectable viable cells. Full article

To achieve a krill meal of high quality, a two-stage drying involving hot-air drying and vacuum drying was investigated. Five experimental groups were established according to the different drying conditions in the second stage, including 95 °C and 101 kPa, 95 °C and 60 kPa, 75 °C and 101 kPa, 75 °C and 60 kPa, and 75 °C and 20 kPa. The results showed that reducing the drying temperature and vacuum pressure in the second stage had a significant impact on the drying characteristics, sensory quality, and bioactive compounds of krill meal. Among all five groups, the drying condition of 75 °C and 60 kPa maintained a high drying rate while preserving a phospholipid content of 30.01 mg/kg and an astaxanthin content of 37.41 mg/kg. It also effectively reduced the isomerization of astaxanthin and the oxidation of unsaturated fatty acids. These results suggested that the two-stage drying method may contribute to the production of high-quality krill meal. Full article

Recent research has demonstrated the positive impact of herbal tea consumption on postprandial blood glucose regulation. This study conducts a comparative analysis of aqueous and ethanol extractions on four herbal teas (Mallotus, Cyclocarya, Rubus, and Vine) to assess their phytochemical profiles and functional attributes. Phytochemical contents, antioxidant activities, α-glucosidase inhibitory activities, and chemical compositions are investigated via colorimetric analyses and UPLC-Q-Orbitrap HRMS/MS, respectively. Results indicate that Vine, among the teas studied, exhibits the most pronounced glucose-regulating effects under both extraction methods. While ethanol extractions yield higher phytochemical content overall, the compositions vary. Conversely, aqueous extracts demonstrate unexpectedly potent antioxidant activities and comparable α-glucosidase inhibitory activities to ethanol extracts. Phytochemical contents correlate positively with antioxidant activities and α-glucosidase inhibitory activities. However, antioxidant activities exhibit a weak positive correlation with α-glucosidase inhibitory activities. These findings provide evidence that aqueous extracts from herbal teas contain valuable phytochemical compositions beneficial for antioxidants and individuals with hyperglycemia, suggesting their potential as functional ingredients to enhance the nutritional value of herbal food products. Full article

To thoroughly understand the profile of phenolic phytochemicals in kidney bean seeds cultivated in a cold region, the extractions, contents, antioxidant activities, compositions of free and bound phenols in the seed coat and cotyledon, and also relevant color attributes, were investigated. The results indicated that ultrasound-assisted extraction was an efficient method for free phenols. The bound phenols in seed coat and cotyledon were released more efficiently by alkali-acid and acid-alkali sequential hydrolysis, respectively. Under the optimized extractions, total phenols (TPC), flavonoids (TFC), and anthocyanins (TAC) ranged in 7.81–32.89 mg GAE/g dw, 3.23–15.65 mg RE/g dw, and 0–0.21 mg CE/g dw in the whole seeds of the five common kidney beans. There was a big difference in phenolic distribution between red and white seeds. From whole seed, the phenols in the four red cultivars mainly existed in free state (78.84%) and seed coat (71.56%), while the phenols in the white ‘Sark’ divided equally between free (51.18%) and bound (48.82%) states and consisted chiefly in cotyledon (81.58%). The correlation analyses showed that the antioxidant activities were significantly and positively correlated with TPC and TFC. The phenolic attributes were closely associated with the color of the seed coat. Red seeds had higher total contents of phenols than white seeds. TAC had a positively significant correlation with redness. Brightness and yellowness showed a negatively significant correlation with TPC, TFC, and antioxidant capacities, which were necessarily linked with redness degree and spot in red seeds. The spotted red ‘Yikeshu’ with the most outstanding performance on phenolic attributes was selected to analyze phenolic compounds with UHPLC-QE-MS. Among the 85 identified phenolics, 2 phenolic acids and 10 flavonoids were dominant. The characteristic phenolics in free and bound states were screened in both seed coat and cotyledon, respectively. The available information on the phenolic profile may expand the utilization of kidney beans as a nutritional ingredient in the food industry. Full article

Salmonella Enteritidis, Escherichia coli, and Campylobacter jejuni are among the most common foodborne pathogens worldwide, and poultry products are strongly associated with foodborne pathogen outbreaks. These pathogens are capable of producing biofilms on several surfaces used in the food processing industry, including polyethylene and stainless steel. However, studies on multi-species biofilms are rare. Therefore, this study aimed to develop predictive mathematical models to simulate the adhesion and removal of multispecies biofilms. All combinations of microorganisms resulted in biofilm formation with differences in bacterial counts. E. coli showed the greatest ability to adhere to both surfaces, followed by S. Enteritidis and C. jejuni. The incubation time and temperature did not influence adhesion. Biofilm removal was effective with citric acid and benzalkonium chloride but not with rhamnolipid. Among the generated models, 46 presented a significant coefficient of determination (R²), with the highest R² being 0.88. These results provide support for the poultry industry in creating biofilm control and eradication programs to avoid the risk of contamination of poultry meat. Full article