Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype and is associated with high recurrence rates, a high incidence of distant metastases and poor overall survival. The aim of this study was to investigate the role of PD-L1, EGFR and AR expression in TNBC promotion and progression. To that end, we analyzed the immunohistochemical expression of these genes in 125 TNBC patients and their relation to clinicopathological parameters and survival. An elevated expression of PD-L1 was significantly correlated with higher tumor and nuclear grade, while a low expression was correlated with loco-regional recurrence without any influence on survival. Contrary to this, the expression of AR showed a positive impact on the DFI and a negative association with tumor grade. Furthermore, PD-L1 and AR demonstrated simultaneous expression, and further co-expression analysis revealed that a positive expression of PD-L1/AR notably correlates with tumor and nuclear grade and has a significant impact on a longer DFI and OS, while a negative PD-L1/AR expression is significantly associated with metastases. Therefore, our results suggest that positive PD-L1/AR expression is beneficial for TNBC patients. In addition, an elevated expression of EGFR contributes to metastases and a worse DFI and OS. In conclusion, we think that low PD-L1/low AR/high EGFR expression followed by high Ki67 expression constitutes a ‘high risk’ profile of TNBC. Full article

Pulsed electric field (PEF) treatment is known as a method that can intensify heat- and mass-transfer-based processes such as osmotic dehydration, drying, or freeze-drying. However, the literature about its impact on quality of freeze-dried products is limited to a few raw materials. The aim of this study was to analyze the effect of PEF on the cell disintegration index, selected bioactive compounds, and physical quality parameters of freeze-dried pumpkin. The final quality of the freeze-dried product was evaluated by residual moisture content, color analysis, total phenolic content, total carotenoid content, sugars content, and hygroscopic properties. The application of PEF treatment induced the disintegration of pumpkin cells even at low energy input (0.11 kJ/kg), and the saturation level of electroporation was reached after 4 kJ/kg. PEF treatment at 2 kJ/kg allowed 40% more total carotenoids to be retained in comparison to the untreated sample. Furthermore, all PEF-treated freeze-dried pumpkin samples exhibited lower sucrose content but had higher glucose and fructose contents in comparison to the reference samples. However, this effect was more pronounced when the shelf temperature was equal to 40 °C. Full article

This study examined 430 Chinese college students’ engagement in arts activities and the psychological benefits derived from such activities. The research differentiated between various types of arts participation and ways of involvement and examined four potential positive psychological outcomes. The findings revealed correlations between (1) creative participation in the performing arts, ‘flow’, and aesthetic emotions; (2) consumptive participation in the visual arts and aesthetic emotions; and (3) creative participation in the literary arts and ego identity. Holistic arts participation demonstrated a significantly positive relationship with flourishing. A path analysis showed that flow experience and aesthetic emotions served as mediators in the mechanism through which holistic arts participation affected flourishing, with a chained mediation effect from flow experience to ego identity. This study confirms that arts participation is an effective pathway for individual flourishing and that more diverse and profound engagement in the arts can lead to sustained and widespread happiness. Full article

This study proposes a probabilistic quantification technique that applies an expert inference method to warn of the risk of a fire developing into a thermal runaway when a lithium-ion battery fire occurs. Existing methods have the shortcomings of low prediction accuracy and delayed responses because they determine a fire only by detecting the temperature rise and smoke in a lithium-ion battery to initiate extinguishing activities. To overcome such shortcomings, this study proposes a method to probabilistically calculate the risk of thermal runaway in advance by detecting the amount of off-gases generated in the venting stage before thermal runaway begins. This method has the advantage of quantifying the probability of a fire in advance by applying an expert inference method based on a combination of off-gas amounts, while maintaining high reliability even when the sensor fails. To verify the validity of the risk probability design, problems with the temperature and off-gas increase/decrease data were derived under four SOC conditions in actual lithium-ion batteries. Through the foregoing, it was confirmed that the risk probability can be accurately presented even in situations where the detection sensor malfunctions by applying an expert inference method to calculate the risk probability complexly. Additionally, it was confirmed that the proposed method is a method that can lead to quicker responses to thermal runaway fires. Full article

The discrete elemental parameters of pod pepper seeds were calibrated for future numerical optimization of the pod pepper seed cleaning device. The study concentrates on calibrating the intrinsic and contact parameters of pod pepper seeds utilizing the discrete element method. Compression tests were performed to ascertain intrinsic parameters such as Poisson’s ratio and the seeds’ elastic modulus. The static friction coefficient and collision restitution coefficient between the seeds and steel plates were identified through incline and free-fall tests. Plackett–Burman, steepest ascent, and Box–Behnken experiments were performed to establish a second-order regression model correlating significant parameters with the angle of repose. The optimal parameter combination, based on the measured angle of repose (32.45°), yielded static friction coefficients between seeds, rolling friction coefficients between seeds, and static friction coefficients between seeds and steel plates of 0.608, 0.018, and 0.787, respectively. The two-sample t-test of the physical and simulated repose angles yielded p > 0.05, and the relative error of the physical and simulated repose angles was 0.68%, which confirmed the reliability of the calibration parameters. The findings indicate that the calibration method for pod pepper seeds effectively informs the calibration of parameters for other irregular crops. Full article

The major issue of raw materials’ depletion, and more specifically, of phosphorous (an important fertilizer) has currently become an emergent aspect due to expected depletion problems needing immediate handling. This was the reason for the implementation of the PhoReSe project that aimed to remove and recover phosphorus from the secondary (biologically treated) effluent of a municipal wastewater (biological) treatment plant (WWTP “AINEIA”, located near Thessaloniki, N. Greece), treating the wastewaters of the nearby touristic area. Regarding the phosphorous supplementary removal and recovery treatment options, two methods were examined, initially at the laboratory scale (batch experiments), i.e., (1) the adsorption of phosphorous, and (2) the chemical precipitation of phosphorus. Both methods were further applied at the pilot scale by initially performing the adsorption of phosphorous onto the AquAsZero commercial sorbent, which is a mixed manganese iron oxy-hydroxide, followed by the chemical precipitation of phosphorous implemented after the desorption process of the previously saturated adsorbent. The final precipitate of this procedure was examined as an alternative/supplementary fertilizer, this way returning phosphorus into the natural cycle. These experiments, as applied successfully in at the pilot scale, set the basis for larger-scale relevant applications for similar WWTP facilities. Full article

In hazardous environments like mining sites, mobile inspection robots play a crucial role in condition monitoring (CM) tasks, particularly by collecting various kinds of data, such as images. However, the sheer volume of collected image samples and existing noise pose challenges in processing and visualizing thermal anomalies. Recognizing these challenges, our study addresses the limitations of industrial big data analytics for mobile robot-generated image data. We present a novel, fully integrated approach involving a dimension reduction procedure. This includes a semantic segmentation technique utilizing the pre-trained VGG16 CNN architecture for feature selection, followed by random forest (RF) and extreme gradient boosting (XGBoost) classifiers for the prediction of the pixel class labels. We also explore unsupervised learning using the PCA-K-means method for dimension reduction and classification of unlabeled thermal defects based on anomaly severity. Our comprehensive methodology aims to efficiently handle image-based CM tasks in hazardous environments. To validate its practicality, we applied our approach in a real-world scenario, and the results confirm its robust performance in processing and visualizing thermal data collected by mobile inspection robots. This affirms the effectiveness of our methodology in enhancing the overall performance of CM processes. Full article

Respiratory burst oxidase homologs (RBOHs), also known as NADPH oxidases, contribute significantly to the production of ROS in plants, alongside other major sources such as photosynthesis and electron transport in chloroplasts. It has been shown that plant RBOHs play an active role in plant adversity response and electron transport. However, the phylogenetic analysis and characterization of the SlRBOH gene family in tomatoes have not been systematically studied. This study identified 11 SlRBOH genes in the tomato genome using a genome-wide search approach. The physicochemical properties, chromosomal localization, subcellular localization, secondary structure, conserved motifs, gene structure, phylogenetics, collinear relationships, cis-acting elements, evolutionary selection pressures, tissue expressions, and expression patterns under exogenous phytohormones (ABA and MeJA) and different abiotic stresses were also analyzed. We found that the SlRBOHs are distributed across seven chromosomes, collinearity reflecting their evolutionary relationships with corresponding genes in Arabidopsis thaliana and rice. Additionally, all the SlRBOH members have five conserved domains and 10 conserved motifs and have similar gene structures. In addition, the results of an evolutionary selection pressure analysis showed that SlRBOH family members evolved mainly by purifying selection, making them more structurally stable. Cis-acting element analyses showed that SlRBOHs were responsive to light, hormone, and abiotic stresses. Tissue expression analysis showed that SlRBOH family members were expressed in all tissues of tomato to varying degrees, and most of the SlRBOHs with the strongest expression were found in the roots. In addition, the expressions of tomato SlRBOH genes were changed by ABA, MeJA, dark period extension, NaCl, PEG, UV, cold, heat, and H₂O₂ treatments. Specifically, SlRBOH4 was highly expressed under NaCl, PEG, heat, and UV treatments, while SlRBOH2 was highly expressed under cold stress. These results provide a basis for further studies on the function of SlRBOHs in tomato. Full article

Binge watching, which entails consecutively viewing numerous episodes of a TV series or a selection of movies over an extended timeframe, often without pause, is notably widespread among young adults skilled in digital media usage. Nevertheless, this escalating habit can lead to problematic and addictive behavior, with potential associations including disruptions in sleep patterns, dreaming, and an elevated risk of suicide. The primary objective of this research was to assess whether daytime sleepiness and maladaptive daydreaming may impact the connection between binge watching and suicide risk and whether the hypothesized model is gender invariant. Self-report questionnaires were administered to a sample of 1012 emerging adults (50% girls) aged 18–25 (M = 21.73, SD = 2.28). Multivariate analysis of covariance (MANCOVA), structural equation modeling (SEM) with latent variables, and multiple-group path analysis (MGPA) were conducted. All direct and indirect paths were found to be statistically significant, and gender was found to be invariant. Specifically, the findings highlighted that higher binge watching, coupled with daytime sleepiness and maladaptive daydreaming, heightened suicide risk in both genders. Binge watching and sleep and dream issues are important factors to consider in the assessment of suicide risk, particularly among emerging adults who are already at a higher risk. It is thus important to prioritize the promotion of healthy media habits. Full article

Genomic prediction plays an increasingly important role in modern animal breeding, with predictive accuracy being a crucial aspect. The classical linear mixed model is gradually unable to accommodate the growing number of target traits and the increasingly intricate genetic regulatory patterns. Hence, novel approaches are necessary for future genomic prediction. In this study, we used an illumina 50K SNP chip to genotype 4190 egg-type female Rhode Island Red chickens. Machine learning (ML) and classical bioinformatics methods were integrated to fit genotypes with 10 economic traits in chickens. We evaluated the effectiveness of ML methods using Pearson correlation coefficients and the RMSE between predicted and actual phenotypic values and compared them with rrBLUP and BayesA. Our results indicated that ML algorithms exhibit significantly superior performance to rrBLUP and BayesA in predicting body weight and eggshell strength traits. Conversely, rrBLUP and BayesA demonstrated 2–58% higher predictive accuracy in predicting egg numbers. Additionally, the incorporation of suggestively significant SNPs obtained through the GWAS into the ML models resulted in an increase in the predictive accuracy of 0.1–27% across nearly all traits. These findings suggest the potential of combining classical bioinformatics methods with ML techniques to improve genomic prediction in the future. Full article

A certified reference material of ricin (CRM-LS-1) was produced by the EuroBioTox consortium to standardise the analysis of this biotoxin. This study established the N-glycan structures and proportions including their loci and occupancy of ricin CRM-LS-1. The glycan profile was compared with ricin from different preparations and other cultivars and isoforms. A total of 15 different oligomannosidic or paucimannosidic structures were identified in CRM-LS-1. Paucimannose was mainly found within the A-chain and oligomannose constituted the major glycan type of the B-chain. Furthermore, the novel primary structure variants E138 and D138 and four different C-termini of the A-chain as well as two B-chain variants V250 and F250 were elucidated. While the glycan proportions and loci were similar among all variants in CRM-LS-1 and ricin isoforms D and E of all cultivars analysed, a different stoichiometry for isoforms D and E and the amino acid variants were found. This detailed physicochemical characterization of ricin regarding the glycan profile and amino acid sequence variations yields unprecedented insight into the molecular features of this protein toxin. The variable attributes discovered within different cultivars present signature motifs and may allow discrimination of the biotoxin’s origin that are important in molecular forensic profiling. In conclusion, our data of in-depth CRM-LS-1 characterization combined with the analysis of other cultivars is representative for known ricin variants. Full article

Allogeneic hematopoietic cell transplantation (allo-HCT) is potentially curative for patients with acute myeloid leukemia (AML). However, the post-transplant relapse rate ranges from 40 to 70%, particularly with reduced intensity conditioning, and remains a major cause of treatment failure for these patients due to the limited efficacy of salvage therapy options. Strategies to mitigate this risk are urgently needed. In the past few years, the basic framework of post-transplant maintenance has been shaped by several clinical trials investigating targeted therapy, chemotherapy, and immunomodulatory therapies. Although the practice of post-transplant maintenance in AML has become more common, there remain challenges regarding the feasibility and efficacy of this strategy. Here, we review major developments in post-transplant maintenance in AML, along with ongoing and future planned studies in this area, outlining the limitations of available data and our future goals. Full article

Blood selenium (Se) concentrations differ substantially by population and could be influenced by genetic variants, increasing Se deficiency-related diseases. We conducted a genome-wide association study (GWAS) to identify single nucleotide polymorphisms (SNPs) associated with serum Se deficiency in 382 adults with admixed ancestry. Genotyping arrays were combined to yield 90,937 SNPs. R packages were applied to quality control and imputation. We also performed the ancestral proportion analysis. The Search Tool for the Retrieval of Interacting Genes was used to interrogate known protein–protein interaction networks (PPIs). Our ancestral proportion analysis estimated 71% of the genome was from Caucasians, 22% was from Africans, and 8% was from East Asians. We identified the SNP rs1561573 in the TraB domain containing 2B (TRABD2B), rs425664 in MAF bZIP transcription factor (MAF), rs10444656 in spermatogenesis-associated 13 (SPATA13), and rs6592284 in heat shock protein nuclear import factor (HIKESHI) genes. The PPI analysis showed functional associations of Se deficiency, thyroid hormone metabolism, NRF2-ARE and the Wnt pathway, and heat stress. Our findings show evidence of a genetic association between Se deficiency and metabolic pathways indirectly linked to Se regulation, reinforcing the complex relationship between Se intake and the endogenous factors affecting the Se requirements for optimal health. Full article

The microstructure–property relationship in poly(methyl methacrylate) PMMA composites is very important for understanding interface phenomena and the future prediction of properties that further help in designing improved materials. In this research, field emission scanning electron microscopy (FESEM) images of denture PMMA composites with SrTiO₃, MnO₂ and SrTiO₃/MnO₂ were used for fractal reconstructions of particle agglomerates in the polymer matrix. Fractal analysis represents a valuable mathematical tool for the characterization of the microstructure and finding correlation between microstructural features and mechanical properties. Utilizing the mathematical affine fractal regression model, the Fractal Real Finder software was employed to reconstruct agglomerate shapes and estimate the Hausdorff dimensions (HD). Controlled energy impact and tensile tests were used to evaluate the mechanical performance of PMMA-MnO₂, PMMA-SrTiO₃ and PMMA-SrTiO₃/MnO₂ composites. It was determined that PMMA-SrTiO₃/MnO₂ had the highest total absorbed energy value (E_tot), corresponding to the lowest HD value of 1.03637 calculated for SrTiO₃/MnO₂ agglomerates. On the other hand, the highest HD value of 1.21521 was calculated for MnO₂ agglomerates, while the PMMA-MnO₂ showed the lowest E_tot. The linear correlation between the total absorbed impact energy of composites and the HD of the corresponding agglomerates was determined, with an R² value of 0.99486, showing the potential use of this approach in the optimization of composite materials’ microstructure–property relationship. Full article

The Tibetan Plateau (TP) is a climate-sensitive and ecologically fragile area. Studying drought and its effects on vegetation over the TP is of great significance for ecological conservation. However, there were large uncertainties in previous studies on the drought characteristics and their impacts on alpine vegetation in this region. This study explored the drought changes and their impacts on alpine vegetation during the growing season over the TP in 1982–2018. The results showed that the TP has experienced a wetting trend in most regions of the TP. Correspondingly, the vegetation has become greener in most areas. The wetting and drying trend in the growing season changed around 1995. Before 1995, the TP experienced an overall drying trend with a spatial pattern of a drying trend in the northern regions and a wetting trend in the southern regions, while it showed an overall wetting trend after 1995, with a reversed spatial pattern to that before 1995. After 1995, wetting and drying trends affected the vegetation in 61% of the TP. However, before 1995, the NDVI presented an increasing trend in most areas of the TP under a drying trend. Therefore, a drying trend was not the primary factor affecting vegetation growth in this period. Instead, changes in the cryosphere induced by warming could be the main factor. In addition, the distribution of vegetation across the TP was primarily influenced by drought intensity, which had the greatest impact on sparse vegetation, followed by meadow and grassland. This study enhances our understanding of the impact of drought changes on alpine vegetation on the TP. Full article

The ever-growing prominence and widespread acceptance of organic light-emitting diodes (OLEDs), particularly those employing thermally activated delayed fluorescence (TADF), have firmly established them as formidable contenders in the field of lighting technology. TADF enables achieving a 100% utilization rate and efficient luminescence through reverse intersystem crossing (RISC). However, the effectiveness of TADF-OLEDs is influenced by their high current density and limited device lifetime, which result in a significant reduction in efficiency. This comprehensive review introduces the TADF mechanism and provides a detailed overview of recent advancements in the development of host-free white OLEDs (WOLEDs) utilizing TADF. This review specifically scrutinizes advancements from three distinct perspectives: TADF fluorescence, TADF phosphorescence and all-TADF materials in host-free WOLEDs. By presenting the latest research findings, this review contributes to the understanding of the current state of host-free WOLEDs, employing TADF and underscoring promising avenues for future investigations. It aims to serve as a valuable resource for newcomers seeking an entry point into the field as well as for established members of the WOLEDs community, offering them insightful perspectives on imminent advancements. Full article

Cellulose nanofibrils (CNFs) are particles with a high aspect ratio. Typically, chemically pre-treated CNFs (containing anionic or cationic charged groups) consist of long fibrils (up to 2 μm) with very low thickness (less than 10 nm). Derived from their high aspect ratio, CNFs form strong hydrogels with high elasticity at low concentrations. Thus, CNF suspensions appear as an interesting rheology modifier to be applied in cosmetics, paints, foods, and as a mineral suspending agent, among other applications. The high viscosity results from the strong 3D fibril network, which is related to the good fibrillation of the material, allowing the nanofibrils to overlap. The overlap concentration (c*) was found to vary from ca. 0.13 to ca. 0.60 wt.% depending on the type and intensity of the pre-treatment applied during the preparation of the CNFs. The results confirm the higher tendency for the fibres treated with (3-chloro-2-hydroxypropyl) trimethylammonium chloride (CHPTAC) and 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO) to form a 3D network, resulting in the lowest c*. For the TEMPO-oxidised CNF suspensions, it was also found that aggregation is improved at acidic pH conditions due to lower charge repulsion among fibrils, leading to an increase in the suspension viscosity as well as higher apparent yield stresses. TEMPO CNF suspensions with a low content of carboxylic groups tend to precipitate at moderately acidic pH values. Full article

Analysis of the heavy fractions in crude oil has been important in petroleum industries. It is well known that heavy fractions such as vacuum gas oils (VGOs) include heteroatoms, of which sulfur and nitrogen are often characterized in many cases. We conducted research regarding the molecular species analysis of VGOs. Further refine processes using VGOs are becoming important when considering carbon recycling. In this work, we attempted to classify compounds within VGOs provided by Kuwait Institute for Scientific Research. Two VGOs were priorly distillated from Kuwait Export crude and Lower Fars crude. Quantitative analysis was performed mainly using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOFMS). MALDI-TOF-MS has been developed for analyzing high-molecular-weight compounds such as polymer and biopolymers. As matrix selection is one of the most important aspects in MALDI-TOFMS, the careful selection of a matrix was firstly evaluated, followed by analysis using a Kendrick plot with nominal mass series (z*). The objective was to evaluate if this work could provide an effective classification of VGOs compounds. The Kendrick plot is a well-known method for processing mass data. The difference in the Kendrick mass defect (KMD) between C_nH_2n−14S and C_nH_2n−20O is only 0.0005 mass units, which makes it difficult in general to distinguish these compounds. However, since the z* value showed effective differences during the classification of these compounds, qualitative analysis could be possible. The analysis using nominal mass series showed the potential to be used as an effective method in analyzing heavy fractions. Full article

Enhancing the aerobic stability of whole-plant corn silage is essential for producing high-quality silage. Our research assessed the effect of inoculation with Lactobacillus buchneri or Bacillus licheniformis and its modulation of the bacterial and fungal microbial community structure in an aerobic stage of whole-plant corn silage. Following treatment with a distilled sterile water control, Lactobacillus buchneri, and Bacillus licheniformis (2 × 10⁵ cfu/g), whole-plant corn was ensiled for 60 days. Samples were taken on days 0, 3, and 7 of aerobic exposure, and the results showed that inoculation with Lactobacillus buchneri or Bacillus licheniformis improved the aerobic stability of silage when compared to the effect of the control (p < 0.05). Inoculation with Bacillus licheniformis attenuated the increase in pH value and the decrease in lactic acid in the aerobic stage (p < 0.05), reducing the filamentous fungal counts. On the other hand, inoculation with Lactobacillus buchneri or Bacillus licheniformis increased the diversity of the fungal communities (p < 0.05), complicating the correlation between bacteria or fungi, reducing the relative abundance of Acetobacter and Paenibacillus in bacterial communities, and inhibiting the tendency of Monascus to replace Issatchenkia in fungal communities, thus delaying the aerobic spoilage process. Due to the prevention of the development of aerobic spoilage microorganisms, the silage injected with Lactobacillus buchneri or Bacillus licheniformis exhibited improved aerobic stability. Full article

Ultraviolet (UV) radiation has been widely utilized as a disinfection strategy to effectively eliminate various pathogens. The disinfection task achieves complete coverage of object surfaces by planning the motion trajectory of autonomous mobile robots and the UVC irradiation strategy. This introduces an additional layer of complexity to path planning, as every point on the surface of the object must receive a certain dose of irradiation. Nevertheless, the considerable dosage required for virus inactivation often leads to substantial energy consumption and dose redundancy in disinfection tasks, presenting challenges for the implementation of robots in large-scale environments. Optimizing energy consumption of light sources has become a primary concern in disinfection planning, particularly in large-scale settings. Addressing the inefficiencies associated with dosage redundancy, this study proposes a dose coverage planning framework, utilizing MOPSO to solve the multi-objective optimization model for planning UVC dose coverage. Diverging from conventional path planning methodologies, our approach prioritizes the intrinsic characteristics of dose accumulation, integrating a UVC light efficiency factor to mitigate dose redundancy with the aim of reducing energy expenditure and enhancing the efficiency of robotic disinfection. Empirical trials conducted with autonomous disinfecting robots in real-world settings have corroborated the efficacy of this model in deactivating viruses. Full article

BBX protein is a class of zinc finger transcription factors that have B-box domains at the N-terminus, and some of these proteins contain a CCT domain at the C-terminus. It plays an important role in plant growth, development, and metabolism. However, the expression pattern of BBX genes in alfalfa under hormonal and salt stresses is still unclear. In this study, we identified a total of 125 BBX gene family members by the available Medicago reference genome in diploid alfalfa (Medicago sativa spp. Caerulea), a model plant (M. truncatula), and tetraploid alfalfa (M. sativa), and divided these members into five subfamilies. We found that the conserved motifs of BBXs of the same subfamily reveal similarities. We analyzed the collinearity relationship and duplication mode of these BBX genes and found that the expression pattern of BBX genes is specific in different tissues. Analysis of the available transcriptome data suggests that some members of the BBX gene family are involved in multiple abiotic stress responses, and the highly expressed genes are often clustered together. Furthermore, we identified different expression patterns of some BBX genes under salt, ethylene, salt and ethylene, salicylic acid, and salt and salicylic acid treatments, verified by qRT-PCR, and analyzed the subcellular localization of MsBBX2, MsBBX17, and MsBBX32 using transient expression in tobacco. The results showed that BBX genes were localized in the nucleus. This study systematically analyzed the BBX gene family in Medicago plants, which provides a basis for the study of BBX gene family tolerance to abiotic stresses. Full article

In the last decade, “expressions” of grape marc spirits aged in wooden barrels of characteristic amber color and complex sensory attributes have been introduced. Yet studies on constituents migrating from the barrel to the beverage are scarce, and their metabolic profile remains unexplored. Furthermore, the literature on the assessment of their antioxidant activity is limited. NMR metabolomics and spectrophotometry have been implemented in 38 samples to elucidate the impact of the aging procedure on the metabolites’ composition and establish whether these beverages exhibit antioxidant activity. Provenance was related to fusel alcohols, esters, acetaldehyde, methanol, saccharides, and 2-phenylethanol, while ethyl acetate and ethyl lactate contributed to discriminating samples of the same winery. Identified metabolites such as vanillin, syringaldehyde, and sinapaldehyde were related to the aging procedure. The maturation in the barrel was also associated with an increase in xylose, glucose, fructose, and arabinose. The antioxidant potential of the aged Greek grape marc spirits resulting from their maturation in oak barrels was highlighted. The metabolic profiling and antioxidant potential of aged Greek grape marc spirits were assessed for the first time. Finally, the enrichment of the aromatic region was noted with the presence of metabolites with a furanic and phenolic ring derived, respectively, from the polysaccharides’ degradation or the thermal decomposition of lignin. Full article