Chang’e-6 (CE-6) is the first sample-return mission from the lunar farside and will be launched in May of 2024. The landing area is in the south of the Apollo basin inside the South Pole Aitken basin. Statistics and analyses of impact craters in the landing area are essential to support safe landing and geologic studies. In particular, the crater size–frequency distribution information of the landing area is critical to understanding the provenance of the CE-6 lunar samples to be returned and can be used to verify and refine the lunar chronology model by combining with the radioisotope ages of the relevant samples. In this research, a digital orthophoto map (DOM) mosaic with resolution of 3 m/pixel of the CE-6 landing area was generated from the 743 Narrow Angle Camera of the Lunar Reconnaissance Orbiter Camera. Based on the DOM, craters were extracted by an automated method and checked manually. A total of 770,731 craters were extracted in the whole area of 246 km × 135 km, 511,484 craters of which were within the mare area. Systematic analyses of the crater distribution, completeness, spatial density, and depth-to-diameter ratio were conducted. Geologic model age estimation was carried out in the mare area that was divided into three geologic units according to the TiO₂ abundance. The result showed that the east part of the mare had the oldest model age of $\mu {3.27}_{-0.045}^{+0.036}$ Ga, and the middle part of the mare had the youngest model age of $\mu {2.49}_{-0.073}^{+0.072}$ Ga. The crater catalogue and the surface model age analysis results were used to support topographic and geologic analyses of the pre-selected landing area of the CE-6 mission before the launch and will contribute to further scientific researches after the lunar samples are returned to Earth. Full article

Extracellular vesicles (EVs) can be isolated from biological fluids and cell culture medium. Their nanometric dimension, relative stability, and biocompatibility have raised considerable interest for their therapeutic use as delivery vehicles of macromolecules, namely nucleic acids and proteins. Deficiency in lysosomal enzymes and associated proteins is at the basis of a group of genetic diseases known as lysosomal storage disorders (LSDs), characterized by the accumulation of undigested substrates into lysosomes. Among them, GM2 gangliosidoses are due to a deficiency in the activity of lysosomal enzyme β-hexosaminidase, leading to the accumulation of the GM2 ganglioside and severe neurological symptoms. Current therapeutic approaches, including enzyme replacement therapy (ERT), have proven unable to significantly treat these conditions. Here, we provide evidence that the lysosomal β-hexosaminidase enzyme is associated with EVs released by HEK cells and that the EV-associated activity can be increased by overexpressing the α-subunit of β-hexosaminidase. The delivery of EVs to β-hexosaminidase-deficient fibroblasts results in a partial cross-correction of the enzymatic defect. Overall findings indicate that EVs could be a source of β-hexosaminidase that is potentially exploitable for developing therapeutic approaches for currently untreatable LSDs. Full article

Background: Individuals with hyperinsulinemia may initially not meet any diagnostic criteria for metabolic syndrome, though displaying a higher risk of cardiovascular complications combined with obesity, diabetes, and hypertension. Aim: The main objective of our study was to assess the diagnostic accuracy of various cardiovascular risk indices in hyperinsulinemic children and adolescents; a secondary objective was to estimate the optimal cut-offs of these indices. Patients and methods: This retrospective single-center study was conducted on 139 patients aged 12.1 ± 2.9 years, managed for hyperinsulinism. Results: We found statistically significant differences in homeostasis model assessment of insulin resistance index (HOMA-IR), triglyceride glucose index (TyG), TyG-body mass index, visceral adiposity index, lipid accumulation product index, fatty liver index, and hepatic steatosis index. At the linear logistic regression assessment, we found that insulin growth factor-1 (IGF-1), HOMA-IR, and ALT/AST ratio were independently associated with confirmed hyperinsulinism. At the multivariate analysis, IGF-1 levels over 203 ng/mL and HOMA-IR higher than 6.2 were respectively associated with a 9- and 18-times higher odds ratio for hyperinsulinism. The other investigated parameters were not significantly related to hyperinsulinism, and could not predict either the presence of hyperinsulinemia or a subsequent cardiovascular risk in our patients. Conclusion: Commonly used indices of cardiovascular risk in adults cannot be considered accurate in confirming hyperinsulinism in children, with the exception of HOMA-IR. Further studies are needed to verify the usefulness of specific cardiovascular risk indices in hyperinsulinemic children and adolescents. Full article

The integration of laser scanning technology and Building Information Modelling (BIM) processes offers a transformative approach to managing the complexities in live construction projects. This paper aims to explore the significant impacts of incorporating laser scanning and BIM on construction projects in terms of as-built models, information management, and overall project performance utilising case study analysis of a building that was not BIM-based. The research scope is defined by the need to investigate the integration of laser scanning and BIM in live construction projects. It details the data acquisition process, challenges encountered due to site obstructions, and the methodologies employed for spatial modelling procedures. Key findings reveal that such integration can significantly enhance the accuracy of data collection and improve project outcomes. Results also identify the need for specialised equipment and skills for the effective implementation of such integrations. The research concludes by offering a practical approach to enhancing construction processes, from design to maintenance. This paper contributes to the body of knowledge by providing a detailed analysis of the practical application of laser scanning and BIM in a live construction project, offering insights into the benefits, challenges, and future directions for integrating these technologies in the construction industry. Full article

Sulfites play imperative roles in food crops and food products, serving as sulfur nutrients for food crops and as food additives in various foods. It is necessary to develop an effective method for the on-site quantification of sulfites in food samples. Here, 7-(diethylamino) quinoline is used as a fluorescent group and electron donor, alongside the pyridinium salt group as an electron acceptor and the C=C bond as the sulfite-specific recognition group. We present a novel fluorescent sensor based on a mechanism that modulates the efficiency of intramolecular charge transfer (ICT), CY, for on-site quantitative measurement of sulfite in food. The fluorescent sensor itself exhibited fluorescence in the near-infrared light (NIR) region, effectively minimizing the interference of background fluorescence in food samples. Upon exposure to sulfite, the sensor CY displayed a ratiometric fluorescence response (I₄₄₇/I₆₉₂) with a high sensitivity (LOD = 0.061 μM), enabling accurate quantitative measurements in complex food environments. Moreover, sensor CY also displayed a colorimetric response to sulfite, making sensor CY measure sulfite in both fluorescence and colorimetric dual-signal modes. Sensor CY has been utilized for quantitatively measuring sulfite in red wine and sugar with recoveries between 99.65% and 101.90%, and the RSD was below 4.0%. The sulfite concentrations in live cells and zebrafish were also monitored via fluorescence imaging. Moreover, the sulfite assimilated by lettuce leaves was monitored, and the results demonstrated that excessive sulfite in leaf tissue could lead to leaf tissue damage. In addition, the sulfate-transformed sulfite in lettuce stem tissue was tracked, providing valuable insights for evaluating sulfur nutrients in food crops. More importantly, to accomplish the on-site quantitative measurement of sulfite in food samples, a portable sensing system was prepared. Sensor CY and the portable sensing system were successfully used for the on-site quantitative measurement of sulfite in food. Full article

Background: We assessed the pertinence of updating the International Working Group on the Diabetic Foot (IWGDF) risk classification yearly in people with diabetes by quantifying the changes in the risk group and its accuracy in identifying those developing an ulcer (DFU) in a primary care setting. Methods: In our retrospective cohort study, we included all people with diabetes with a foot assessment registry between January 2016 and December 2018 in the Baixo Alentejo Local Health Unit. Foot-related data were collected at baseline after one and two years. DFU and/or death until December 2019 were registered. The proportion of people changing their risk status each year was calculated. Accuracy measures of the IWGDF classification to predict DFU occurrence at one, two, and three years were calculated. Results: A total of 2097 people were followed for three years, during which 0.1% died and 12.4% developed a DFU. After two years, 3.6% of the participants had progressed to a higher-risk group. The IWGDF classification presented specificity values superior to 90% and negative predictive values superior to 99%. Conclusion: Foot risk status can be safely updated every two years instead of yearly, mainly for those at very low risk. The IWGDF classification can accurately identify those not at risk of DFU. Full article

Hydrangea macrophylla, a celebrated ornamental worldwide, thrives in semi-shaded growth environments in its natural habitat. This study utilizes Hydrangea macrophylla ‘Endless Summer’ as the experimental material to delve into its molecular mechanisms for adapting to semi-shaded conditions. Transcriptome analysis was conducted on leaves from four different natural light growth scenarios, showcasing phenotypic variations. From each sample, we obtained over 276,305,940 clean reads. Following de novo assembly and quantitative assessment, 88,575 unigenes were generated, with an average length of 976 bp. Gene ontology analysis of each control group elucidated the terms associated with the suitable environmental conditions for normal growth, development, and flowering, such as “reproductive bud system development” and “signal transduction”. The exploration of gene interactions and the identification of key genes with strong connectivity were achieved by constructing a protein–protein interaction (PPI) network. The results indicate that hydrangea grows vigorously and blooms steadily under semi-shaded conditions; the photosynthetic efficiency of hydrangea is stabilized through genes related to photosynthesis, such as PHYB, PSBR, FDC, etc. Hormone signal transduction genes like PIN3, LAX2, TIF6B, and EIN3 play important roles in responding to environmental stimulation and regulating growth and development, while genes such as SOC1, COL4/5/16, and AGL24 promote flowering. The expression of genes such as BGLUs and TPSs provides additional energy substances to support flowering. Full article

Telerehabilitation is an appealing service delivery option for optimising recovery. Internationally, the equity of telerehabilitation services for people from culturally and linguistically diverse (CALD) backgrounds has been questioned. Using a 31-item survey, our study explored the access, readiness and willingness of 260 patients receiving allied health services from a large tertiary health service located in Sydney, Australia, to use telerehabilitation for adults. Overall, 72% patients reported having access to technology, 38% met our readiness criteria and 53% reported willingness to engage in telerehabilitation. There were no differences in access, readiness and willingness to engage in telerehabilitation between patients from CALD and non-CALD backgrounds. Age was the only factor that influenced access (OR = 0.94, 95% CI 0.90 to 0.97), readiness (OR = 0.95, 95% CI 0.92 to 0.98) and willingness (OR = 0.97, 95% CI 0.95 to 1.00) to engage in telerehabilitation. Past experience of telerehabilitation was related to willingness (OR = 2.73, 95% CI 1.55–4.79) but not access (OR = 1.79, 95% CI 0.87 to 3.68) or readiness (OR = 1.90, 95% CI 0.93 to 3.87). Our findings highlight the importance of ensuring positive patient experiences to promote ongoing willingness to use telerehabilitation. Efforts are needed to improve patients’ digital health literacy, especially patients from older age groups, to ensure equitable engagement in telerehabilitation services. Full article

Background: Suboptimal influenza and pneumococcal vaccination rates have been reported before the COVID-19 pandemics in certain populations at risk for severe infection. The aim of this longitudinal cohort study was to investigate changes in influenza and pneumococcal vaccination rates and patient perceptions in patients with psoriasis (PsO) before and during the pandemic. Methods: Data on vaccination, patient and disease characteristics, comorbidity, and patient perceptions were collected with questionnaires before and during the pandemic approximately one year later. Results: Over the whole cohort who participated in the follow-up visit (n = 287; 59.2% male; mean age: 56.3 years), both influenza and pneumococcal lifetime vaccination prevalences increased significantly from 50.5% to 66.2% and from 16.0% to 41.5%, respectively. A total of 88.5% of PsO patients were interested in a COVID-19 vaccination or had already received it. The reasons for and against vaccinations changed significantly before and during the pandemic. Conclusions: Despite a promising increase in the vaccination prevalence in our PsO cohort, it remains important that awareness for vaccinations is encouraged and closely monitored in future research, particularly in populations at risk. Full article

Surface roughness is one of the main bases for measuring the surface quality of machined parts. A large amount of training data can effectively improve model prediction accuracy. However, obtaining a large and complete surface roughness sample dataset during the ultra-precision machining process is a challenging task. In this article, a novel virtual sample generation scheme (PSOVSGBLS) for surface roughness is designed to address the small sample problem in ultra-precision machining, which utilizes a particle swarm optimization algorithm combined with a broad learning system to generate virtual samples, enriching the diversity of samples by filling the information gaps between the original small samples. Finally, a set of ultra-precision micro-groove cutting experiments was carried out to verify the feasibility of the proposed virtual sample generation scheme, and the results show that the prediction error of the surface roughness prediction model was significantly reduced after adding virtual samples. Full article

The COVID-19 outbreak has impacted many daily activities and services we depend on. Due to changes in waste quantity and types, solid waste management (SWM) services such as waste collection, transportation, and treatment/disposal suffered. Global rules and mandates were issued to address these changes and the COVID-19 pandemic. This mini review examines seven countries and summarises the pandemic’s effects on municipal solid waste (MSW) and medical waste (MW) generation in terms of amount and composition, the SWM sector’s challenges, and government or other SWM guidelines and management measures. The data are analysed to provide suggestions for stakeholders on SWM worker protection, waste segregation, and recycling. This article identified that extending MW incineration, separating infectious waste at the source, and discontinuing recycling for infection control are the best ways to manage solid waste. The waste management system’s readiness was crucial to the pandemic response. Thus, countries like China, which has a robust SWM system, were able to contain the crisis and restrict danger, while others with weaker systems struggled. Additionally, the study highlights the importance of revising waste management policies and developing crisis response strategies that integrate flexible, innovative solutions to adapt quickly to changing waste demands and ensure public health and environmental protection during global health crises. Full article

Organic solar cells (OSCs) are one of the most promising photovoltaic technologies due to their affordability and adaptability. However, upscaling is a critical issue that hinders the commercialization of OSCs. A significant challenge is the lack of cost-effective and facile techniques to modulate the morphology of the active layers. The slow solvent evaporation leads to an unfavorable phase separation, thus resulting in a low power conversion efficiency (PCE) of organic solar modules. Here, a nitrogen-blowing assisted method is developed to fabricate a large-area organic solar module (active area = 12 cm²) utilizing high-boiling-point solvents, achieving a PCE of 15.6%. The device fabricated with a high-boiling-point solvent produces a more uniform and smoother large-area film, and the assistance of nitrogen-blowing accelerates solvent evaporation, resulting in an optimized morphology with proper phase separation and finer aggregates. Moreover, the device fabricated by the nitrogen-blowing assisted method exhibits improved exciton dissociation, balanced carrier mobility, and reduced charge recombination. This work proposes a universal and cost-effective technique for the fabrication of high-efficiency organic solar modules. Full article

We report on a highly significant, positive association between anthrax vaccination and occurrence of Gulf War Illness (GWI) in 111 Gulf War veterans (42 with GWI and 69 controls). GWI was diagnosed in 47.1% of vaccinated veterans but only in 17.2% of non-vaccinated veterans (Pearson χ² = 7.08, p = 0.008; odds ratio = 3.947; relative risk = 2.617), with 1.6x higher GWI symptom severity in vaccinated veterans (p = 0.007, F-test in analysis of covariance). Next, we tested the hypothesis that the susceptibility to GWI following anthrax vaccination could be due to inability to make antibodies against the anthrax protective antigen (PA), the key protein contained in the vaccine. Since the first step in initiating antibody production would be the binding of PA peptide fragments (typically 15-amino acid long [15-mer]) to peptide-binding motifs of human leukocyte antigen (HLA) Class II molecules, we assessed the binding-motif affinities of such HLA specific molecules to all linear 15-mer peptide fragments of the anthrax PA. We identified a total of 58 HLA Class II alleles carried by the veterans in our sample and found that, of those, 18 (31%) were present in the vaccinated group that did not develop GWI but were absent from the vaccinated group who developed GWI. Remarkably, in silico analyses revealed very high binding affinities of peptide-binding motifs of those 18 HLA alleles with fragments of anthrax vaccine PA, leading to the successful production of anti-PA antibodies. Conversely, the absence of these protective HLA alleles points to a reduced ability to develop antibodies against PA, thus resulting in harmful PA persistence and development of GWI. Full article

Teachers report a need for professional development in order to feel more confident and be more effective in providing writing instruction and intensive intervention to support middle school students. This study investigates the impacts of online practice-based professional development (PBPD) and coaching for self-regulated strategy development (SRSD) argumentative writing instruction on teachers’ implementation of the instruction. Using a pre-test–post-test design, the study also examined the effects of the SRSD instruction on students’ argumentative writing from source texts. Five middle school teachers from three regions across the United States participated in the PBPD and implemented SRSD with 55 middle school students with high-incidence disabilities or below-proficient writing skills. Following PBPD, teachers implemented the SRSD argumentative writing instruction with high adherence to dosage, fidelity of implementation, and quality. Student writing was scored for argumentative genre elements, holistic quality, total words written, and transition words. Results demonstrate that students improved on all measures. In addition, students reported feeling confident in their argumentative writing abilities. Teachers and students reported high social validity for the intervention. The benefits found in this research study provide a strong foundation supporting the application of comprehensive online PBPD with follow-up coaching to support teacher implementation of SRSD. Full article

Personalized sleep medicine represents a transformative shift in healthcare, emphasizing individualized approaches to optimizing sleep health, considering the bidirectional relationship between sleep and health. This field moves beyond conventional methods, tailoring care to the unique physiological and psychological needs of individuals to improve sleep quality and manage disorders. Key to this approach is the consideration of diverse factors like genetic predispositions, lifestyle habits, environmental factors, and underlying health conditions. This enables more accurate diagnoses, targeted treatments, and proactive management. Technological advancements play a pivotal role in this field: wearable devices, mobile health applications, and advanced diagnostic tools collect detailed sleep data for continuous monitoring and analysis. The integration of machine learning and artificial intelligence enhances data interpretation, offering personalized treatment plans based on individual sleep profiles. Moreover, research on circadian rhythms and sleep physiology is advancing our understanding of sleep’s impact on overall health. The next generation of wearable technology will integrate more seamlessly with IoT and smart home systems, facilitating holistic sleep environment management. Telemedicine and virtual healthcare platforms will increase accessibility to specialized care, especially in remote areas. Advancements will also focus on integrating various data sources for comprehensive assessments and treatments. Genomic and molecular research could lead to breakthroughs in understanding individual sleep disorders, informing highly personalized treatment plans. Sophisticated methods for sleep stage estimation, including machine learning techniques, are improving diagnostic precision. Computational models, particularly for conditions like obstructive sleep apnea, are enabling patient-specific treatment strategies. The future of personalized sleep medicine will likely involve cross-disciplinary collaborations, integrating cognitive behavioral therapy and mental health interventions. Public awareness and education about personalized sleep approaches, alongside updated regulatory frameworks for data security and privacy, are essential. Longitudinal studies will provide insights into evolving sleep patterns, further refining treatment approaches. In conclusion, personalized sleep medicine is revolutionizing sleep disorder treatment, leveraging individual characteristics and advanced technologies for improved diagnosis, treatment, and management. This shift towards individualized care marks a significant advancement in healthcare, enhancing life quality for those with sleep disorders. Full article

The formation and evolution of management gullies is a highly intense process of soil erosion often overlooked in policies and river basin strategies. Despite the worldwide spread of the phenomenon, our ability to assess and simulate gullying and its impacts remains limited; therefore, predicting the development and evolution of these river reaches represents a significant challenge, especially in areas where the loss of productive soil or the hazards linked to landslides or floods represent critical factors. Our study demonstrates how an exclusively morphometric approach, based on the construction of the hypsometric curve and applied to small hydrographic basins that are lithologically homogeneous and hierarchized according to the Strahler classification method, is able to predict the triggering height of the gullies; this height corresponds to the mean elevation of the basin and the inflection point of the hypsometric curve itself, confirming the hypothesis that this point coincides with the point at which a sudden change in surface runoff energy occurs, The study also shows that the portion of the basin necessary to trigger these intense erosive processes is always within a small range, regardless of the size and morphology (slope) of the basin itself. Such an approach, which is quick and relatively easy to apply, could help develop hydrogeological hazard mitigation practices in land planning projects. Full article

Transdermal drug delivery offers a promising alternative for administering medications like ibuprofen, known for its analgesic and anti-inflammatory properties, with reduced gastrointestinal side effects compared to oral administration. This study explored the potential synergistic effects of combining ibuprofen with lavender essential oil (LEO) in transdermal patches. The composition of LEO was analyzed, revealing predominant compounds such as linalyl acetate and linalool, which are known for their analgesic and anti-inflammatory properties. The physicochemical properties of the patches were investigated, indicating improved cohesion with the addition of LEO. Additionally, thermal stability assessments demonstrated enhanced stability with LEO incorporation with an increase in onset decomposition temperature from 49.0 to 67.9 °C. The antioxidant activity of patches containing LEO was significantly higher with a free radical scavenging ability of 79.13% RSA compared to 60% RSA in patches without LEO. Release and permeation studies showed that patches with LEO exhibited an increased permeation of ibuprofen through the skin with 74.40% of the drug released from LEO-containing patches compared to 36.29% from patches without LEO after 24 h. Moreover, the permeation rate was notably faster with LEO, indicating quicker therapeutic effects. The inclusion of LEO in transdermal patches containing ibuprofen holds promise for enhancing drug delivery efficiency and therapeutic effectiveness, offering a potential strategy for improved pain management with reduced side effects. Full article

This study investigates the impact of Epstein–Barr virus (EBV) infection on children’s proteomes across different phases of the disease, utilising seventy-nine blood samples categorised into three groups: EBV-naive patients, acute infectious mononucleosis (IM) cases, and convalescents followed up for 12 months post-IM. The aim is to identify proteins influenced by EBV infection, shedding light on the chronic processes triggered by the virus. The results reveal thirty-nine proteins distinguishing between naive patients and those with IM, including actin, lumican, peroxiredoxin-2, fibulin-1, gelsolin, and alpha-2-macroglobulin, which are involved in immune responses, cell adhesion, and inflammation. Elevated oxidative stress markers like peroxiredoxin-2 in IM patients suggest potential links to EBV’s induction of reactive oxygen species. Increased levels of apolipoproteins A-I, A-IV, C-IV, and M during IM imply associations with viral infection, while complement system proteins (C1q, C1r, and C8 gamma chain) are also elevated, reflecting their role in the immune response and viral clearance. This study’s focus on children provides unique insights into EBV’s impact on young populations, emphasising proteomics’ role in uncovering protein associations and understanding the virus’s long-term consequences. However, specific relationships between identified proteins and EBV infection require further investigation. Full article

This study aimed to investigate near-infrared spectroscopy (NIRS) in combination with classification methods for the discrimination of fresh and once- or twice-freeze–thawed fish. An experiment was carried out with common carp (Cyprinus carpio). From each fish, test pieces were cut from the dorsal and ventral regions and measured from the skin side as fresh, after single freezing at minus 18 °C for 15 ÷ 28 days and 15 ÷ 21 days for the second freezing after the freeze–thawing cycle. NIRS measurements were performed via a NIRQuest 512 spectrometer at the region of 900–1700 nm in Reflection mode. The Pirouette 4.5 software was used for data processing. SIMCA and PLS-DA models were developed for classification, and their performance was estimated using the F1 score and total accuracy. The predictive power of each model was evaluated for fish samples in the fresh, single-freezing, and second-freezing classes. Additionally, aquagrams were calculated. Differences in the spectra between fresh and frozen samples were observed. They might be assigned mainly to the O–H and N–H bands. The aquagrams confirmed changes in water organization in the fish samples due to freezing–thawing. The total accuracy of the SIMCA models for the dorsal samples was 98.23% for the calibration set and 90.55% for the validation set. For the ventral samples, respective values were 99.28 and 79.70%. Similar accuracy was found for the PLS-PA models. The NIR spectroscopy and tested classification methods have a potential for nondestructively discriminating fresh from frozen–thawed fish in as methods to protect against fish meat food fraud. Full article

The mechanisms underlying unsatisfactory immune reconstitution in HIV-1 positive patients under ART have not been fully elucidated, even after years of investigation. Thus, this study aimed to assess the correlation between age and thymic production profile, and its influence on inadequate immunological recovery. Here, 44 ART-treated patients with undetectable plasma HIV-1 load (<40 copies/mL) were classified as 31 immunological responders (IR) and 13 immunological non-responders (INR), according to their CD4+ T-cell count after 18 months of ART. The thymic function was assessed by identifying recent thymic emigrants (RTEs) CD4+ T cells (CD4+/CD45RA+CD31+) in PBMCs using flow cytometry. Clinical data were also analyzed from medical records. The INR group showed a higher age at ART initiation (41 ± 3.0) compared to the IR (33.7 ± 2.1) group (p = 0.041). Evaluating RTE CD4+ T-cells, we observed a lower percentage in the INR group (19.5 ± 6.3) compared to the IR group (29.9 ± 11.5) (p = 0.012). There was a strong negative correlation between age at ART initiation and RTE CD4+ T-cells in INRs (r = −0.784, p = 0.004). Our study has highlighted the thymic insufficiency and aging-related immunosenescence with unsatisfactory immunological recovery during ART in HIV-1 positive patients. Full article