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16 pages, 2434 KiB

Open AccessArticle

Improvement of Climate Resource Utilization in the Southwestern Hilly Region through the Construction of a New Multi-Maturing Cropping System

by Fanlei Kong, Tongliang Li, Wei Zhang, Pijiang Yin, Fan Liu, Tianqiong Lan, Dongju Feng, Xinglong Wang and Jichao Yuan

Agronomy 2024, 14(6), 1154; https://doi.org/10.3390/agronomy14061154 (registering DOI) - 28 May 2024

Abstract

The construction of an appropriate cropping pattern is crucial for the improvement of regional agricultural economic efficiency and sustainable development. Despite previous efforts, there remains a gap in optimizing cropping patterns that fully leverage climate resources to enhance production efficiency. This study addresses [...] Read more.

The construction of an appropriate cropping pattern is crucial for the improvement of regional agricultural economic efficiency and sustainable development. Despite previous efforts, there remains a gap in optimizing cropping patterns that fully leverage climate resources to enhance production efficiency. This study addresses this gap by systematically comparing the differences in climate resource allocation, production efficiency and crop response among models by constructing four new triple-maturing cropping models at typical ecological sites in the hilly areas of southwest China. To solve the above problems, we constructed eight cropping patterns and classified them to three as follows: the Traditional Double Cropping System, the Traditional Triple Cropping System, the Novel Triple Cropping System. The results showed that the new multi-maturing planting pattern was significantly better than the traditional two-maturing netting pattern and the traditional three-maturing planting pattern in terms of light, temperature and water productivity. Compared with the traditional two-maturity net cropping model and the traditional three-maturity cropping model, the new cropping model increased light energy productivity by 97.88% and 50.00%, respectively; light energy use by an average of 0.48% and 0.31%; cumulative temperature productivity by an average of 84.70% and 49.14%; and rainfall productivity by an average of 101.04% and 49.61%. An assessment of the light, temperature and water meteorological resource use efficiency of the different treatments showed that the resource use efficiency of the new multi-maturing planting pattern was on average 111.58% and 74.78% higher than that of the traditional two-maturing net planting pattern and the traditional three-maturing planting pattern, with the T6 pattern having the highest resource use efficiency. The new multi-ripening cropping pattern has demonstrated production stability in response to changes in light, temperature and water resources, better adapting to weekly climate changes, stabilizing yields and improving efficiency. In summary, the results of this study can provide a theoretical basis for optimizing cropping patterns and promoting the use of climate resources in agriculture and sustainable development. Future research should focus on further refining these models, exploring their adaptability to various climatic conditions, and evaluating their long-term economic and environmental impacts. Full article

(This article belongs to the Section Innovative Cropping Systems)

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13 pages, 1728 KiB

Open AccessArticle

Influence of Aortic Arch Morphology on Renal Perfusion in Patients with Coarctation of the Aorta: An Exploratory Study

by Sigitas Cesna, Augustinas Bielinis, Tadas Zvirblis, Marius Miglinas and Virgilijus Tarutis

Medicina 2024, 60(6), 886; https://doi.org/10.3390/medicina60060886 - 28 May 2024

Abstract

Objectives: The configuration of the aortic arch, particularly a Gothic arch shape, in individuals with corrected coarctation of the aorta (CoA) has been associated with a decreased systolic wave amplitude across the arch, which could potentially impair renal perfusion and elevate the [...] Read more.

Objectives: The configuration of the aortic arch, particularly a Gothic arch shape, in individuals with corrected coarctation of the aorta (CoA) has been associated with a decreased systolic wave amplitude across the arch, which could potentially impair renal perfusion and elevate the risk of arterial hypertension. This study aims to explore the relationship between the morphological characteristics of the aortic arch and their impact on renal perfusion in patients with CoA. Methods: Seventy-one subjects with corrected CoA underwent continuous 24 h ambulatory blood pressure monitoring, computed tomography to assess the aortic arch, and renal perfusion scanning. Subjects were stratified into three groups based on the height-to-width (H/W) ratio of their aortic arch: Group 1 with a H/W ratio of <0.65, Group 2 with a H/W ratio between 0.65 and 0.85, and Group 3 with a H/W ratio of >0.85. Results: Groups 1 and 2 (53,78% and 62.63%) presented with a higher hypertension prevalence of elevated blood pressure than Group 3 (38.89%). Notable variations were observed among the subjects in the time to peak perfusion (Tmax) in the left kidney across the groups. Group 1 showed a median T_max at 0.27, Group 2 at 0.13, and Group 3 at −0.38 (p-value = 0.079). The differences in T_max for the right kidney followed a similar trend but were not statistically significant (Group 1 at 0.61, Group 2 at 0.22, and Group 3 at 0.11; p-value = 0.229). Conclusions: This study suggests that variations in the aortic arch morphology might not significantly influence renal perfusion in CoA patients. This indicates the potential adaptability of the renal blood flow, which appears to compensate for reduced perfusion, thus minimizing adverse effects on the kidney function. This adaptability suggests an inherent physiological resilience, emphasizing the need for further targeted research to understand the specific interactions and impacts on treatment strategies for CoA. Full article

(This article belongs to the Special Issue Clinical Cardiology in a New Era: Navigating Challenges and Embracing Opportunities)

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6 pages, 1047 KiB

Open AccessCase Report

Primary Renal Angiosarcoma: Rare Tumour with Lethal Outcomes

by Zilvinas Venclovas, Aurelija Alksnyte, Urte Rimsaite, Tomas Navickis, Mindaugas Jievaltas and Daimantas Milonas

Medicina 2024, 60(6), 885; https://doi.org/10.3390/medicina60060885 - 28 May 2024

Abstract

Introduction: Renal haemangioma is a benign tumour, and due to its characteristics, it must be distinguished from malignant diseases. We present a clinical case of primary renal angiosarcoma initially mistaken for haemangioma due to their similarity. Case report: A 58-year-old man [...] Read more.

Introduction: Renal haemangioma is a benign tumour, and due to its characteristics, it must be distinguished from malignant diseases. We present a clinical case of primary renal angiosarcoma initially mistaken for haemangioma due to their similarity. Case report: A 58-year-old man was admitted to the hospital with suspicion of pulmonary embolism. The patient complained of pain on the left side. An ultrasound and CT scan of the abdomen showed a tumour mass ~20.5 × 17.2 × 15.4 cm in size in the projection of the left kidney. On CT images, there were data for clear cell renal clear cell carcinoma (ccRCC). A left nephrectomy was performed. However, histological examination revealed renal haemangioma. Three months later, the patient presented to the hospital with abdominal and lumbar pain. A CT scan showed multiple small hypoechoic foci up to 2 cm in size in the liver, lungs, and intra-abdominally, with the most data for carcinosis. Histological re-verification of the left kidney showed a renal vascular tumour with pronounced signs of infarction and necrosis with the majority of the evidence supporting angiosarcoma. Despite treatment, the patient’s outcome was fatal. Conclusions: Based on the clinical presentation, radiological images and histological examination data, the tumour was initially misdiagnosed as kidney haemangioma. Due to the rarity of this tumour, there are no established treatment protocols or clinical guidelines for managing primary kidney angiosarcoma. Full article

(This article belongs to the Section Oncology)

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13 pages, 343 KiB

Open AccessArticle

Unaddressed Challenges in the Treatment of Cutaneous Melanoma?

by Alessia Villani, Luca Potestio, Aimilios Lallas, Zoe Apalla, Massimiliano Scalvenzi and Fabrizio Martora

Medicina 2024, 60(6), 884; https://doi.org/10.3390/medicina60060884 - 28 May 2024

Abstract

Background and Objectives: While the management of noninvasive cutaneous melanoma (CM) is typically limited to a secondary excision to reduce recurrence risk and periodic follow-up, treating patients with advanced melanoma presents ongoing challenges. Materials and Methods: This review provides a comprehensive [...] Read more.

Background and Objectives: While the management of noninvasive cutaneous melanoma (CM) is typically limited to a secondary excision to reduce recurrence risk and periodic follow-up, treating patients with advanced melanoma presents ongoing challenges. Materials and Methods: This review provides a comprehensive examination of both established and emerging pharmacologic strategies for advanced CM management, offering an up-to-date insight into the current therapeutic milieu. The dynamic landscape of advanced CM treatment is explored, highlighting the efficacy of immune checkpoint inhibitors and targeted therapies, either in monotherapy or combination regimens. Additionally, ongoing investigations into novel treatment modalities are thoroughly discussed, reflecting the evolving nature of melanoma management. Results: The therapeutic landscape for melanoma management is undergoing significant transformation. Although various treatment modalities exist, there remains a critical need for novel therapies, particularly for certain stages of melanoma or cases resistant to current options. Conclusions: Consequently, further studies are warranted to identify new treatment avenues and optimize the utilization of existing drugs. Full article

(This article belongs to the Special Issue Chronic Inflammatory Skin Disease: Where We Are and Where We Are Going)

9 pages, 22173 KiB

Open AccessCase Report

Maxillary Osteonecrosis Related with Herpes Zoster: A Case Report and Review of the Literature

by Kwan-Soo Park

Medicina 2024, 60(6), 883; https://doi.org/10.3390/medicina60060883 - 28 May 2024

Abstract

Osteonecrosis of the jaw (ONJ) can occur through various mechanisms including radiation, medication, and viral infections such as herpes zoster. Although herpes zoster is a varicella-zoster virus infection that can affect the trigeminal nerve, it rarely causes oral complications. The author reports a [...] Read more.

Osteonecrosis of the jaw (ONJ) can occur through various mechanisms including radiation, medication, and viral infections such as herpes zoster. Although herpes zoster is a varicella-zoster virus infection that can affect the trigeminal nerve, it rarely causes oral complications. The author reports a rare case of herpes zoster-related ONJ, followed by a review of the relevant literature pertaining to herpes zoster-related oral complications, including ONJ. A 73-year-old woman presented with a scarred skin lesion on her left midface with an exposed alveolar bone of the left maxilla. Based on her medical records, she received a diagnosis and treatment for herpes zoster six months prior and experienced a few teeth loss in the left maxilla following a fall preceding the onset of herpes zoster. Sequestrectomy of the left maxilla was performed and ONJ was diagnosed. The operative site recovered favorably. Although unusual, several cases of localized extensive ONJ in herpes zoster-infected patients have been reported. This case illustrates the possibility of a rare occurrence of unilateral widespread osteonecrosis of the jaw (ONJ) even in the maxilla associated with herpes zoster. The exact mechanism has not been elucidated; nevertheless, surgeons should consider the possibility of oral and dental complications, including ONJ, related to a history of herpes zoster. Full article

(This article belongs to the Section Dentistry)

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12 pages, 5977 KiB

Open AccessArticle

Biomechanical Comparison of Fixation Methods for Posterior Wall Fractures of the Acetabulum: Conventional Reconstruction Plate vs. Spring Plate vs. Variable Angle Locking Compression Plate

by HoeJeong Chung, Hoon-Sang Sohn, Jong-Keon Oh, Sangho Lee and DooSup Kim

Medicina 2024, 60(6), 882; https://doi.org/10.3390/medicina60060882 - 28 May 2024

Abstract

Background and Objectives: Acetabular fractures, though infrequent, present considerable challenges in treatment due to their association with high-energy trauma and poor prognoses. Posterior wall fractures, the most common type among them, typically have a more favorable prognosis compared to other types. Anatomical [...] Read more.

Background and Objectives: Acetabular fractures, though infrequent, present considerable challenges in treatment due to their association with high-energy trauma and poor prognoses. Posterior wall fractures, the most common type among them, typically have a more favorable prognosis compared to other types. Anatomical reduction and stable fixation of the posterior wall are crucial for optimal treatment outcomes. This study aimed to biomechanically compare three commonly used fixation methods for posterior wall fractures of the acetabulum—a conventional reconstruction plate, a spring plate, and a 2.7 mm variable angle locking compression plate (VA-LCP). Materials and Methods: The study utilized 6 fresh-frozen cadavers, yielding 12 hemipelvises free from prior trauma or surgery. Three fixation methods were compared using a simple acetabulum posterior wall fracture model. Fixation was performed by an orthopedic specialist, with prebending of plates to minimize errors. Hemipelvises were subjected to quasi-static and cyclic loading tests, measuring fracture gap, stiffness, and displacement under load. Results: It showed no significant differences in fracture gap among the three fixation methods under cyclic loading conditions simulating walking. However, the conventional reconstruction plate exhibited a greater stiffness compared to the spring and variable angle plates. Fatigue analysis revealed no significant differences among the plates, indicating a similar stability throughout cyclic loading. Despite differences in stiffness, all three fixation methods demonstrated adequate stability under loading conditions. Conclusions: While the conventional reconstruction plate demonstrated a superior stiffness, all three fixation methods provided sufficient stability under cyclic loading conditions similar to walking. This suggests that postoperative limitations are unlikely with any of the three methods, provided excessive activities are avoided. Furthermore, the variable angle plate—like the spring plate—offers an appropriate stability for fragment-specific fixation, supporting its use in surgical applications. These findings contribute to understanding the biomechanical performance of different fixation methods for acetabular fractures, facilitating improved surgical outcomes in challenging cases. Full article

(This article belongs to the Section Orthopedics)

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16 pages, 13981 KiB

Open AccessArticle

Electrical Smoothing of the Powder Bed Surface in Laser-Based Powder Bed Fusion of Metals

by Andreas Hofmann, Tim Grotz, Nico Köstler, Alexander Mahr and Frank Döpper

J. Manuf. Mater. Process. 2024, 8(3), 112; https://doi.org/10.3390/jmmp8030112 - 28 May 2024

Abstract

Achieving a homogeneous and uniform powder bed surface as well as a defined, uniform layer thickness is crucial for achieving reproducible component properties that meet requirements when powder bed fusion of metals with a laser beam. The existing recoating processes cause wear of [...] Read more.

Achieving a homogeneous and uniform powder bed surface as well as a defined, uniform layer thickness is crucial for achieving reproducible component properties that meet requirements when powder bed fusion of metals with a laser beam. The existing recoating processes cause wear of the recoater blade due to protruded, melted obstacles, which affects the powder bed surface quality locally. Impairments to the powder bed surface quality have a negative effect on the resulting component properties such as surface quality and relative density. This can lead either to scrapped components or to additional work steps such as surface reworking. In this work, an electric smoother is presented with which a wear-free and contactless smoothing of the powder bed can be realized. The achievable powder bed surface quality was analyzed using optical profilometry. It was found that the electric smoother can compensate for impairments in the powder bed surface and achieve a reproducible surface quality of the powder bed regardless of the initial extent of the impairments. Consequently, the electric smoother offers a promising opportunity to reduce the scrap rate in PBF-LB/M and to increase component quality. Full article

(This article belongs to the Special Issue Design, Processes and Materials for Additive Manufacturing)

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13 pages, 3701 KiB

Open AccessFeature PaperArticle

Integrating Deep Learning into Genotoxicity Biomarker Detection for Avian Erythrocytes: A Case Study in a Hemispheric Seabird

by Martín G. Frixione, Facundo Roffet, Miguel A. Adami, Marcelo Bertellotti, Verónica L. D’Amico, Claudio Delrieux and Débora Pollicelli

Math. Comput. Appl. 2024, 29(3), 41; https://doi.org/10.3390/mca29030041 - 28 May 2024

Abstract

Recently, nuclear abnormalities in avian erythrocytes have been used as biomarkers of genotoxicity in several species. Anomalous shapes are usually detected in the nuclei by means of microscopy inspection. However, due to inter- and intra-observer variability, the classification of these blood cell abnormalities [...] Read more.

Recently, nuclear abnormalities in avian erythrocytes have been used as biomarkers of genotoxicity in several species. Anomalous shapes are usually detected in the nuclei by means of microscopy inspection. However, due to inter- and intra-observer variability, the classification of these blood cell abnormalities could be problematic for replicating research. Deep learning, as a powerful image analysis technique, can be used in this context to improve standardization in identifying the biological configurations of medical and veterinary importance. In this study, we present a standardized deep learning model for identifying and classifying abnormal shapes in erythrocyte nuclei in blood smears of the hemispheric and synanthropic kelp gulls (Larus dominicanus). We trained three convolutional backbones (ResNet34, ResNet50, and ResNet101 architectures) to obtain models capable of detecting and classifying these abnormalities in blood cells. The analysis was performed at three discrimination levels of classification, with broad categories subdivided into increasingly specific subcategories (level 1: “normal”, “abnormal”, “other”; level 2: “normal”, “ENAs”, “micronucleus”, “other”; level 3: “normal”, “irregular”, “displaced”, “enucleated”, “micronucleus”, “other”). The results were more than adequate and very similar in levels 1 and 2 (F1-score 84.6% and 83.6%, and accuracy 83.9% and 82.6%). In level 3, performance was lower (F1-score 65.9% and accuracy 80.8%). It can be concluded that the level 2 analysis should be considered the most appropriate as it is more specific than level 1, with similar quality of performance. This method has proven to be a fast, efficient, and standardized approach that reduces the dependence on human supervision in the classification of nuclear abnormalities in avian erythrocytes, and can be adapted to be used in similar contexts with reduced effort. Full article

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Graphical abstract

21 pages, 7785 KiB

Open AccessArticle

Experiences Using MEMS Accelerometers on Railway Bearers at Switches and Crossings to Obtain Displacement—Awkward Situations

by Jou-Yi Shih, Paul Weston, Mani Entezami, Clive Roberts and Mark O’Callaghan

Infrastructures 2024, 9(6), 91; https://doi.org/10.3390/infrastructures9060091 - 28 May 2024

Abstract

A sleeper, or more generally a “bearer”, moves vertically under a passing train load. The extent of this motion depends on the static and dynamic load of the train, the train speed, and the support conditions at the bearer and its neighbours. Excessive [...] Read more.

A sleeper, or more generally a “bearer”, moves vertically under a passing train load. The extent of this motion depends on the static and dynamic load of the train, the train speed, and the support conditions at the bearer and its neighbours. Excessive motion, typically from voiding see-sawing, low support stiffness or possibly excessive stiffness, or even too little stiffness, are all of interest to maintainers. Typically, problems arise around transition zones, switches and crossings, but plain track with poor support can also be a problem. Within the last decade, low-cost micro-electro-mechanical system (MEMS) accelerometers have been used to capture the time history of vertical motion for use in condition monitoring. Existing condition monitoring systems often overlook or sometimes even ignore the possibility of problematic data, which seem to be common in monitored locations. It is essential to understand whether such “bad” data require further attention. Three problematic sites are presented, focussing on examples where the acceleration was higher than expected or the computed displacement was not as expected. Potential causes include wheel defects, hammering of the ballast by a hanging bearer, or high acceleration at some structural resonant frequency. The present paper aims to show the challenges of using MEMS accelerometers to collect data for condition monitoring and offers insights into the sort of problematic data that may be collected from real sites. Full article

(This article belongs to the Special Issue Emerging Technologies for Effective and Intelligent Transport Infrastructure Monitoring)

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11 pages, 1671 KiB

Open AccessArticle

Genetic Characterization of Endangered Sterlet (Acipenser ruthenus, Linnaeus 1758) Gene Bank Broodstock, Natural and Cultured Populations in Hungary

by Gyöngyvér Fazekas, Gyula Kovács, Zsuzsanna J. Sándor, Katalin Bogár, Aliz Géczi and Balázs Kovács

Fishes 2024, 9(6), 201; https://doi.org/10.3390/fishes9060201 - 28 May 2024

Abstract

The sterlet (Acipenser ruthenus) is the smallest-bodied endangered species among the six native sturgeon species of the Danube River, and self-sustaining populations still inhabit the Hungarian section of the Danube River and its largest tributary, the Tisza River. Their populations are [...] Read more.

The sterlet (Acipenser ruthenus) is the smallest-bodied endangered species among the six native sturgeon species of the Danube River, and self-sustaining populations still inhabit the Hungarian section of the Danube River and its largest tributary, the Tisza River. Their populations are drastically decreasing; however, they still have natural reproduction in these habitats. For the genetic conservation of the species, an ex situ gene bank is maintained in Hungary. The present study aimed to analyze the genetic resources of a gene bank with a near 40-year history and to compare it with natural populations and farmed stocks. Twelve microsatellites were used for population genetics analyses and individual genotyping of 268 specimens from two natural habitats (Danube and Tisza Rivers) and three captive stocks (a gene bank broodstock and two farms). Microsatellites revealed similar patterns among wild populations and gene bank stocks and did not show genetic differentiation (F_ST: 0.016–0.017) among them. These results confirmed that the gene bank broodstock properly represents the genetic background of the Danube and Tisza populations and is suitable as a source of breeding materials for the restocking programs. Negative trends were detected in the farmed stocks, reflected in reduced polymorphism at a few loci. The results of the principal component analyses indicate the farm stocks’ separation from the wild and gene bank stocks. The present genetic characterization study reveals a valuable captive stock of the endangered sterlet populations and provides unique information about the genetic similarities and differences among farms and wild stocks in Hungary. Our results provide information that contributes to preserving the genetic structure and variability in sterlet populations and supports the management of gene bank broodstock—avoiding inbreeding and preserving the unique genetic background of the Carpathian basin. Full article

(This article belongs to the Section Genetics and Biotechnology)

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7 pages, 187 KiB

Open AccessCommunication

Managing Complexity in Socio-Technical Systems by Mimicking Emergent Simplicities in Nature: A Brief Communication

by Andrea Falegnami, Andrea Tomassi, Giuseppe Corbelli and Elpidio Romano

Biomimetics 2024, 9(6), 322; https://doi.org/10.3390/biomimetics9060322 - 28 May 2024

Abstract

In the context of socio-technical systems, traditional engineering approaches are inadequate, calling for a fundamental change in perspective. A different approach encourages viewing socio-technical systems as complex living entities rather than through a simplistic lens, which enhances our understanding of their dynamics. However, [...] Read more.

In the context of socio-technical systems, traditional engineering approaches are inadequate, calling for a fundamental change in perspective. A different approach encourages viewing socio-technical systems as complex living entities rather than through a simplistic lens, which enhances our understanding of their dynamics. However, these systems are designed to facilitate human activities, and the goal is not only to comprehend how they operate but also to guide their function. Currently, we lack the appropriate terminology. Hence, we introduce two principal concepts, simplexity and complixity, drawing inspiration from how nature conceals intricate mechanisms beneath straightforward, user-friendly interfaces. Full article

(This article belongs to the Special Issue A Systems Approach to BioInspired Design)

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Graphical abstract

15 pages, 4174 KiB

Open AccessArticle

Influence of Screw Design and Process Parameters on the Product Quality of PEO:LiTFSI Solid Electrolytes Using Solvent-Free Melt Extrusion

by Katharina Platen, Frederieke Langer and Julian Schwenzel

Batteries 2024, 10(6), 183; https://doi.org/10.3390/batteries10060183 - 28 May 2024

Abstract

All-solid-state battery (ASSB) technology is a new energy system that reduces the safety concerns and improves the battery performance of conventional lithium-ion batteries (LIB). The increasing demand for such new energy systems makes the transition from laboratory scale production of ASSB components to [...] Read more.

All-solid-state battery (ASSB) technology is a new energy system that reduces the safety concerns and improves the battery performance of conventional lithium-ion batteries (LIB). The increasing demand for such new energy systems makes the transition from laboratory scale production of ASSB components to larger scale essential. Therefore, this study investigates the dry extrusion of poly(ethylene oxide):lithium bis (trifluoromethylsulfonyl)imide (PEO:LiTFSI) all-solid-state electrolytes at a ratio of 20:1 (EO:Li). We investigated the influence of different extruder setups on the product quality. For this purpose, different screw designs consisting of conveying, kneading and mixing elements are evaluated. To do so, a completely dry and solvent-free production of PEO:LiTFSI electrolytes using a co-rotating, intermeshing, twin-screw extruder under an inert condition was successfully carried out. The experiments showed that the screw design consisting of kneading elements gives the best results in terms of process stability and homogeneous mixing of the electrolyte components. Electrochemical impedance spectroscopy was used to determine the lithium-ion conductivity. All electrolytes produced had an ionic conductivity (σ_ionic) of (1.1–1.8) × 10⁻⁴ S cm⁻¹ at 80 °C. Full article

(This article belongs to the Special Issue Advances in Processing, Manufacturing, and Integration of Li-Metal All-Solid-State Batteries)

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14 pages, 1714 KiB

Open AccessFeature PaperArticle

Chemical Variability, Antioxidant and Larvicidal Efficacy of EOs from Citrus sinensis (L.) Osbeck Peel, Leaf, and Flower

by Devi Prasad Bhandari, Pratiksha Chaudhary, Siddha Raj Upadhyaya, Rajeshwor Ranjitkar, Rakesh Satyal, Achyut Adhikari, Prabodh Satyal and Niranjan Parajuli

Horticulturae 2024, 10(6), 566; https://doi.org/10.3390/horticulturae10060566 - 28 May 2024

Abstract

Essential oils (EOs) from Citrus sinensis (Rutaceae) possess diverse biological activities. However, a comprehensive comparison of their chemical composition and bioactivity across different plant parts has not been studied yet. The current research comparatively assesses the yield, chemical composition, chiral distribution, antioxidant properties, [...] Read more.

Essential oils (EOs) from Citrus sinensis (Rutaceae) possess diverse biological activities. However, a comprehensive comparison of their chemical composition and bioactivity across different plant parts has not been studied yet. The current research comparatively assesses the yield, chemical composition, chiral distribution, antioxidant properties, and larvicidal activity of EOs extracted from the peels, leaves, and flowers of C. sinensis. EOs extracted via hydro-distillation (HD) and steam distillation (SD) were analyzed by gas chromatography–mass spectrometry (GC-MS) and chiral GC-MS to explore their chemical composition and enantiomeric distribution. In addition, their larvicidal and antioxidant potentials were evaluated following standard protocols. Peels of C. sinensis exhibited significantly higher oil content (1.75–2.25%) compared to its leaves (0.75–0.78%) and flowers (0.20–0.25%). The GC-MS analysis identified around 60 compounds, including terpenoids, sesquiterpenoids, and oxygenated terpenoids in the HD and SD extractions. Higher concentrations of sabinene were found in flower extract (38.05–39.89%) and leaf extract (32.30–36.91%), while peel extract contained more than 90% limonene. The larvicidal activity of peel oil was primarily attributed to limonene, with an LC₅₀ value of 0.0031 µL/mL. The current study reports the first chiral (GC-MS) analysis in the essential oil of the leaves and flowers of C. sinensis, paving the way for authenticity and purity. Furthermore, the chemical profiling of citrus EOs, particularly from the peel, demonstrates a safe and promising candidate for diverse biological applications. Full article

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10 pages, 4746 KiB

Open AccessArticle

An Environmentally Friendly Method to Protect Box Trees (Buxus spp.) from Attacks by the Invasive Moth Cydalima perspectalis

by Bruno Baur, Werner Gysin and Hans-Peter Rusterholz

Horticulturae 2024, 10(6), 565; https://doi.org/10.3390/horticulturae10060565 - 28 May 2024

Abstract

The non-native invasive box-tree moth Cydalima perspectalis causes severe damage to ornamental box trees (Buxus spp.) and natural boxwood stands. So far, no promising natural enemy of C. perspectalis has been discovered in Europe. Many garden owners would like to protect their [...] Read more.

The non-native invasive box-tree moth Cydalima perspectalis causes severe damage to ornamental box trees (Buxus spp.) and natural boxwood stands. So far, no promising natural enemy of C. perspectalis has been discovered in Europe. Many garden owners would like to protect their box trees from C. perspectalis without the use of insecticides, which also harm other arthropod species. In a controlled experiment under natural conditions, we tested whether box trees covered with a net during the flight period of C. perspectalis are as well protected against the moth as trees treated with a bioinsecticide. After 1 year, the box trees covered with a net during the moths’ flight activity (monitored by light traps) showed no damage by larvae (average loss of leaves 0%), as did box trees regularly treated with a bioinsecticide (control group 1). In contrast, box trees with no protection (untreated box trees; control group 2) lost 97.7% of their leaves due to larval feeding. In a second experiment, we investigated whether defoliated box trees can recover when covered with a net during the flight period of the moth. Protected by the net, the emerging new leaves were not attacked by C. perspectalis. After 1.5 years, the trees had 24% of their original foliage again. Our study demonstrates that temporarily covering box trees with a net protects them against damage by C. perspectalis in an effective way. Full article

(This article belongs to the Section Insect Pest Management)

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17 pages, 2490 KiB

Open AccessArticle

Enhancing the Nutritional Profile of Crataegus monogyna Fruits by Optimizing the Extraction Conditions

by Konstantina Kotsou, Dimitra Magopoulou, Theodoros Chatzimitakos, Vassilis Athanasiadis, Eleni Bozinou, Athanassios I. Sfougaris and Stavros I. Lalas

Horticulturae 2024, 10(6), 564; https://doi.org/10.3390/horticulturae10060564 - 28 May 2024

Abstract

Crataegus monogyna (CM) fruits are highly regarded for their rich nutritional content, boasting elevated levels of various beneficial secondary metabolites like total polyphenols, including anthocyanins, and ample amounts of ascorbic acid and antioxidant activity. Despite the acknowledged benefits of CM fruits, researchers have [...] Read more.

Crataegus monogyna (CM) fruits are highly regarded for their rich nutritional content, boasting elevated levels of various beneficial secondary metabolites like total polyphenols, including anthocyanins, and ample amounts of ascorbic acid and antioxidant activity. Despite the acknowledged benefits of CM fruits, researchers have directed more attention toward its leaves and flowers. Consequently, the current research attempts to optimize extraction techniques for CM fruit using a multifaceted approach involving varied durations, temperatures, and concentrations of ethanol solvent to isolate the diverse range of bioactive components present effectively. High-performance liquid chromatography coupled with a diode array detector (HPLC-DAD) is employed for the identification and quantification of polyphenolic compounds. According to the results, by following the optimum extraction parameters (50% ethanolic solvent, 50 °C extraction temperature, and 60 min extraction time), the total polyphenol content can be increased up to 410%, reaching 55.59 mg gallic acid equivalents/g. Using 50% ethanolic solvent, 80 °C extraction temperature, and extraction time of 90 min, the total anthocyanin content can be enhanced by more than 560%, reaching a quantity of 51.83 μg cyanidin equivalents/g. Moreover, the antioxidant activity of CM fruit extracts can reach 415.95 μmol ascorbic acid equivalents (AAE)/g dw (by FRAP method), using 50% ethanolic solvent, 50 °C extraction temperature, and 60 min extraction time, and 270.26 μmol AAE/g dw (by DPPH method) and 1053.28 mg/100 g dw ascorbic acid content, using 50% ethanolic solvent, 80 °C extraction temperature, and 90 min extraction time. This comprehensive study seeks to augment the already substantial content of bioactive compounds found in CM, resulting in an extract with promising applications across the pharmaceutical, food, and cosmetics industries. Full article

(This article belongs to the Special Issue Bioactive Compounds in Horticultural Plants—2nd Edition)

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24 pages, 990 KiB

Open AccessArticle

Phenotypic Diversity of Pomegranate Cultivars: Discriminating Power of Some Morphological and Fruit Chemical Characteristics

by Mira Radunić, Maja Jukić Špika and Jelena Gadže

Horticulturae 2024, 10(6), 563; https://doi.org/10.3390/horticulturae10060563 - 28 May 2024

Abstract

In modern agricultural production, where a small number of commercial cultivars dominate, the collection, evaluation, and preservation of germplasm are important tasks to reduce the erosion of genes and preserve biodiversity. The aim of this study is to characterize the morphological and fruit [...] Read more.

In modern agricultural production, where a small number of commercial cultivars dominate, the collection, evaluation, and preservation of germplasm are important tasks to reduce the erosion of genes and preserve biodiversity. The aim of this study is to characterize the morphological and fruit chemical properties of the pomegranate germplasm grown on the East Adriatic coast, including the commercial cultivars ‘Hicaznar’, ‘Granada’, and ‘Wonderful’, and to highlight the characteristics with the greatest discriminating power. The characterization of the tree, leaf, flower, arils, seed, and juice was carried out using the UPOV descriptor. The colors of the peel, arils, and juice were analyzed according to the CIEL*a*b* method, total soluble solids were measured using refractometers, and total acidity was determined by titration with 0.1 M NaOH. The research results showed significant diversity between the cultivars, which were grouped into several clusters using an unsupervised analysis technique. Factors such as plant vigor, plant growth habit, predominant number of leaves per node on young shoots, crown type, fruit shape, fruit shape in cross-section, peel weight, total aril weight, aril weight, number of arils per fruit, seed length and width, seed yield, total acidity, TSS/TA ratio, and color parameters of the peel, arils, and juice showed high variability, indicating their strong discriminating power in determining the phenotypic diversity of pomegranate. Full article

(This article belongs to the Special Issue Research on Pomegranate Germplasm, Breeding, Genetics and Multiomics)

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21 pages, 2548 KiB

Open AccessReview

The Macroalga Kappaphycus alvarezii as a Potential Raw Material for Fermentation Processes within the Biorefinery Concept: Challenges and Perspectives

by Adam Tabacof, Verônica Calado and Nei Pereira, Jr.

Fermentation 2024, 10(6), 283; https://doi.org/10.3390/fermentation10060283 - 28 May 2024

Abstract

Seaweed is a fast-growing biomass source that is currently studied as feedstock for sustainable industrial production in a wide variety of markets. Being composed mostly of polysaccharides, macroalgae can be integrated in biorefineries for obtaining bioproducts via fermentation. Kappaphycus alvarezii has been introduced [...] Read more.

Seaweed is a fast-growing biomass source that is currently studied as feedstock for sustainable industrial production in a wide variety of markets. Being composed mostly of polysaccharides, macroalgae can be integrated in biorefineries for obtaining bioproducts via fermentation. Kappaphycus alvarezii has been introduced experimentally to Brazil’s south coastline in 1995 and is now cultivated on a large scale to keep up with the high carrageenan demand in various industrial sectors. In this review article, an introduction is given on renewable biomass and environmental issues, focusing especially on third-generation biomass and its promising features and use advantages. Later on, the processing of K. alvarezii for the use of its saccharide portion for fermentative processes is approached. The current state of research conducted alongside challenges and hurdles in K. alvarezii hydrolysate fermentation processes provides insight into future studies needed to make new fermentation processes viable. Next, some fermentation products are discussed, and the metabolism of galactose in microorganisms is also presented to bring to light other possible fermentation products that are not yet, but can be, obtained from K. alvarezii. Finally, a simple and comprehensive scheme for K. alvarezii fermentation biorefinery is presented to demonstrate a generic example for a possible configuration for obtaining valuable bio-products. In the literature, production of ethanol and lactic acid were already reported from K. alvarezii. This review aims to help envision new industrial processes that can be developed for this most valuable macroalga. Full article

(This article belongs to the Special Issue Algae—the Medium of Bioenergy Conversion: 2nd Edition)

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12 pages, 620 KiB

Open AccessArticle

Characteristics of Isolated Lactic Acid Bacteria and Their Application in High-Moisture Broccoli Waste Silage

by Chenfei Dong and Jie Yuan

Fermentation 2024, 10(6), 282; https://doi.org/10.3390/fermentation10060282 - 28 May 2024

Abstract

Four lactic acid bacteria (LAB) strains isolated from naturally ensiled broccoli waste were characterized, and their effects on the fermentation quality of high-moisture broccoli waste silage were studied. The four isolated strains were assessed using the morphological, physiological and biochemical tests. The four [...] Read more.

Four lactic acid bacteria (LAB) strains isolated from naturally ensiled broccoli waste were characterized, and their effects on the fermentation quality of high-moisture broccoli waste silage were studied. The four isolated strains were assessed using the morphological, physiological and biochemical tests. The four strains were added to broccoli waste at three moisture contents (90%, W0; 87%, W1; 80%, W2) and ensiled for 60 days. All strains (CB89, CB94, CB112, and CB120) grew at 15–45 °C, pH 3.0–7.0, and in 3.0–6.5% NaCl and were identified as Lactiplantibacillus plantarum by 16S rDNA sequencing. Inoculation of CB120 significantly (p < 0.05) increased lactic acid, starch, and non-structural carbohydrate content, and significantly (p < 0.05) decreased pH values and aerobic bacteria count compared with control (CK) at all three moisture contents. In conclusion, CB120 improved the fermentation quality and nutritional value of broccoli waste silage at three moisture contents and could be applied as a promising additive for high-moisture material. Full article

(This article belongs to the Special Issue The Use of Lactobacillus in Forage Storage and Processing)

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15 pages, 2728 KiB

Open AccessArticle

Utilization of the Dicarbonyl Compounds 3-Deoxyglucosone and 3-Deoxymaltosone during Beer Fermentation by Saccharomyces Yeasts

by Anna-Lena Kertsch, Michael Brysch-Herzberg, Kai Ostermann and Thomas Henle

Fermentation 2024, 10(6), 281; https://doi.org/10.3390/fermentation10060281 - 28 May 2024

Abstract

In beer production, 1,2-dicarbonyl compounds such as 3-deoxyglucosone (3-DG) and 3-deoxymaltosone (3-DM) are formed via Maillard reaction or caramelization especially during malt kilning or wort boiling, resulting in substantial concentrations in wort. Consequences of dicarbonyl compounds for yeast metabolism are widely unknown. In [...] Read more.

In beer production, 1,2-dicarbonyl compounds such as 3-deoxyglucosone (3-DG) and 3-deoxymaltosone (3-DM) are formed via Maillard reaction or caramelization especially during malt kilning or wort boiling, resulting in substantial concentrations in wort. Consequences of dicarbonyl compounds for yeast metabolism are widely unknown. In the present study, the handling of 3-DG and 3-DM by Saccharomyces strains from different habitats in wort and during beer fermentation was investigated. We show that beer yeast strains induced a faster 3-DG degradation in Pilsner wort and were additionally more stress-resistant to 3-DG compared to yeasts isolated from natural habitats. In fermentation experiments comparing a light wort and a dark wort prepared from malt extracts, it could be shown that high levels of 3-DM in dark wort influence the utilization of 3-DG by yeasts, and thus higher levels of 3-DG remain in the wort. Beer yeast strains showed an increased formation of 3-deoxyfructose (3-DF) with up to 220 µM, which is possibly due to a preferred metabolization of 3-DM, as indicated by the low degradation rate of 3-DG. In contrast, yeasts isolated from natural habitats produced significantly lower amounts of 3-DF. This suggests an adaptation of technologically used yeasts to metabolization of dicarbonyl compounds, possibly as a result of beer yeast domestication. Full article

(This article belongs to the Special Issue Applied and Fundamental Studies of Yeast in Fermented Foods and Beverages)

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23 pages, 3899 KiB

Open AccessArticle

Enhancing Load-Bearing Capacity of Calcareous Sands through Gel Stabilization: A Mechanical and Material Characterization Study

by Jianxiao Gu, Haibo Lyu, Bo Li, Yong Wang, Hui Chen, Xinyi Gao and Xiaojiang Xu

Gels 2024, 10(6), 373; https://doi.org/10.3390/gels10060373 - 28 May 2024

Abstract

Calcareous sands often display wide ring grain configurations, high intragranular porosity, a complex structure, and low grain hardness. These attributes typically do not meet the strength criteria necessary to sustain overlying infrastructure in civil engineering applications. This study investigates gel stabilization techniques, blending [...] Read more.

Calcareous sands often display wide ring grain configurations, high intragranular porosity, a complex structure, and low grain hardness. These attributes typically do not meet the strength criteria necessary to sustain overlying infrastructure in civil engineering applications. This study investigates gel stabilization techniques, blending gel material with calcareous sand at concentrations ranging from 5% to 22%, followed by curing periods of 3 to 28 days to evaluate the load-bearing capacity. Subsequently, an unconfined compressive test is performed to determine the gel material content in stabilized specimens and investigate the influence of gel material types. The gel material-to-sand ratios employed are set at 5%, 10%, and 16% for Portland cement and 13%, 16%, and 22% for gypsum. After that, a triaxial consolidated undrained test is conducted to assess mechanical behavior, pore water pressure, and mechanical properties. The findings reveal increased dilation, stress–strain hardening, and softening post-yield, regardless of gel material type. Principal stress ratios, secant modulus, and cohesion show a positive correlation with maintenance duration and binder content, with implications for improved load-bearing capacity. The study also elucidates the qualitative relationship between secant modulus E₅₀ and confining pressure. Full article

(This article belongs to the Special Issue Novel Polymer Gels: Synthesis, Properties, and Applications)

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16 pages, 3707 KiB

Open AccessReview

Progress of Research into Preformed Particle Gels for Profile Control and Water Shutoff Techniques

by Wei Ma, Yikun Li, Pingde Liu, Zhichang Liu and Tao Song

Gels 2024, 10(6), 372; https://doi.org/10.3390/gels10060372 - 28 May 2024

Abstract

Gel treatment is an economical and efficient method of controlling excessive water production. The gelation of in situ gels is prone to being affected by the dilution of formation water, chromatographic during the transportation process, and thus controlling the gelation time and penetration [...] Read more.

Gel treatment is an economical and efficient method of controlling excessive water production. The gelation of in situ gels is prone to being affected by the dilution of formation water, chromatographic during the transportation process, and thus controlling the gelation time and penetration depth is a challenging task. Therefore, a novel gel system termed preformed particle gels (PPGs) has been developed to overcome the drawbacks of in situ gels. PPGs are superabsorbent polymer gels which can swell but not dissolve in brines. Typically, PPGs are a granular gels formed based on the crosslinking of polyacrylamide, characterized by controllable particle size and strength. This work summarizes the application scenarios of PPGs and elucidates their plugging mechanisms. Additionally, several newly developed PPG systems such as high-temperature-resistant PPGs, re-crosslinkable PPGs, and delayed-swelling PPGs are also covered. This research indicates that PPGs can selectively block the formation of fractures or high-permeability channels. The performance of the novel modified PPGs was superior to in situ gels in harsh environments. Lastly, we outlined recommended improvements for the novel PPGs and suggested future research directions. Full article

(This article belongs to the Special Issue Applications of Gels for Enhanced Oil Recovery)

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Graphical abstract

11 pages, 2552 KiB

Open AccessArticle

Transcutaneous Auricular Vagal Nerve Stimulation in Healthy Non-Sedated Horses: A Feasibility Study

by Valentina Vitale, Francesca Bindi, Ana Velloso Alvarez, María de la Cuesta-Torrado, Giulia Sala and Micaela Sgorbini

Vet. Sci. 2024, 11(6), 241; https://doi.org/10.3390/vetsci11060241 - 28 May 2024

Abstract

This study aimed to evaluate the feasibility of transcutaneous auricular vagal nerve stimulation (tAVNS) in healthy horses and its effect on heart rate variability (HRV). The study comprised three phases: the selection of mares, their acclimatization to the tAVNS, and the stimulation phase. [...] Read more.

This study aimed to evaluate the feasibility of transcutaneous auricular vagal nerve stimulation (tAVNS) in healthy horses and its effect on heart rate variability (HRV). The study comprised three phases: the selection of mares, their acclimatization to the tAVNS, and the stimulation phase. Stimulation was performed with two electrodes positioned on the right pinna. The settings were 0.5 mA, 250 μs, and 25 Hz for pulse amplitude, pulse width, and pulse frequency, respectively. HRV was analysed before (B1), during (T), and after (B2) the tAVNS. From the 44 mares initially included, only 7 completed the three phases. In these mares, the heart rate (HR) was significantly lower, and frequency domain parameters showed an increased parasympathetic tone in B2 compared with B1. However, in 3/7 mares, the HR was significantly higher during T compared with B1 and B2, compatible with a decreased parasympathetic tone, while in 4/7 mares, the HR was significantly lower and the parasympathetic nervous system index was significantly higher during T and B2 compared with B1. The tAVNS is an economical and easy procedure to perform and has the potential to stimulate vagal activity; however, it was poorly tolerated in the mares included in this study. Full article

(This article belongs to the Topic Application of Animal Models: From Physiology to Pathology)

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15 pages, 418 KiB

Open AccessArticle

Determinants of Humanities and Social Sciences Students’ Intentions to Use Artificial Intelligence Applications for Academic Purposes

by Konstantinos Lavidas, Iro Voulgari, Stamatios Papadakis, Stavros Athanassopoulos, Antigoni Anastasiou, Andromachi Filippidi, Vassilis Komis and Nikos Karacapilidis

Information 2024, 15(6), 314; https://doi.org/10.3390/info15060314 (registering DOI) - 28 May 2024

Abstract

Recent research emphasizes the importance of Artificial Intelligence applications as supporting tools for students in higher education. Simultaneously, an intensive exchange of views has started in the public debate in the international educational community. However, for a more proper use of these applications, [...] Read more.

Recent research emphasizes the importance of Artificial Intelligence applications as supporting tools for students in higher education. Simultaneously, an intensive exchange of views has started in the public debate in the international educational community. However, for a more proper use of these applications, it is necessary to investigate the factors that explain their intention and actual use in the future. With the Unified Theory of Acceptance and Use of Technology (UTAUT2) model, this work analyses the factors influencing students’ use and intention to use Artificial Intelligence technology. For this purpose, a sample of 197 Greek students at the School of Humanities and Social Sciences from the University of Patras participated in a survey. The findings highlight that expected performance, habit, and enjoyment of these Artificial Intelligence applications are key determinants influencing teachers’ intentions to use them. Moreover, behavioural intention, habit, and facilitating conditions explain the usage of these Artificial Intelligence applications. This study did not reveal any moderating effects. The limitations, practical implications, and proposed directions for future research based on these results are discussed. Full article

(This article belongs to the Special Issue Artificial Intelligence and Games Science in Education)

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