Vaccination has emerged as the most effective tool in the battle against COVID-19. To optimize vaccination protocols, a deeper understanding of the immune response to vaccination, including influential factors and its duration, is essential. This study aimed to assess the humoral response in vaccinated individuals with or without prior SARS-CoV-2 infection. A prospective observational study was conducted across 14 private healthcare structures in Greece. Anti-spike IgG titers were measured at different timepoints following the initial vaccination and booster doses of the BNT162b2, mRNA-1273, ChAdOx1 nCoV-19, and Ad26.COV2.S vaccines. A total of 505 participants were included in the first phase, evaluating the humoral response after the initial vaccination, and 311 participants were involved in the second phase, assessing the effects of booster vaccination. All vaccines elicited high anti-S IgG titers initially, followed by a subsequent decline that was addressed by the booster vaccination. The humoral response was sustained up to one year after the booster vaccination. mRNA vaccines induced higher anti-S IgG titers compared to vector vaccines, with mRNA-1273 eliciting higher titers than BNT162b2. Vaccination resulted in higher antibody titers than natural infection alone; however, convalescent patients who received vaccination had significantly higher anti-S IgG titers compared to those who received the booster vaccine without previous SARS-CoV-2 infection. Lower antibody titers were observed in men and older patients (>51.5 years old), as well as smokers, although the decline rate was lower in these subgroups. These results underscore the importance of booster doses and reveal the potential influence of age, gender, smoking habits, and vaccine type on varying humoral responses. Long-term monitoring of antibody persistence, evaluation of cellular immune responses, and assessment of vaccine efficacy against emerging variants should be considered to enhance our understanding of immunity dynamics and inform vaccine development and deployment strategies. Full article

Objective: To assess the quality and readability of online information on holmium laser enucleation of the prostate in managing benign prostate hyperplasia using the most-used search engine worldwide, Google. Methods: Google search terms “Holmium laser surgery” and “enlarged prostate” were used to generate 150 search results. Two independent authors (i) excluded any paywall, scientific literature, or advertisement and (ii) conducted an independent assessment on information quality, which was based on DISCERN, QUEST, and JAMA criteria, and readability, which was based on the FKG, GFI, SMOG, and FRE scores on qualified webpages. A third author was involved if there were any discrepancies between the assessments. Results: 107 qualified webpages were included in the data analysis. The median DISCERN score was 42 out of 80 (IQR 35–49). The median JAMA score was 0 out of 4 (IQR 0–1). The median QUEST score was 9 out of 28 (IQR 9–12). Using the non-parametric ANOVA and post hoc Games–Howell test, significant differences were identified between rankings of webpages. Sponsorship had no influence on the quality of webpages. The overall readability level required a minimum reading level of grade 11. Linear regression analysis showed that a higher ranked webpage is a positive predictor for all three quality assessment tools. Conclusions: The overall quality of online information on HoLEP is poor. We identify that the top-ranked google searches have a higher DISCERN score and are a positive predictor for DISCERN/QUEST/JAMA. Quality online information can benefit patients but should be used in conjunction with professional medical consultation. Full article

TikTok has become a hub for easily accessible medical information. However, the quality and completeness of this information for testicular cancer has not been examined. Our study aims to assess the quality and completeness of testicular cancer information on TikTok. A search was performed on TikTok using the search terms “Testicular Cancer” and “Testicle Cancer”. Inclusion criteria encompassed videos about testicular cancer in English. We excluded non-English videos, irrelevant videos, and videos without audio. We evaluated these videos using the DISCERN instrument and a completeness assessment. A total of 361 videos were considered for screening and 116 videos were included. Of these, 57 were created by healthcare professionals (HCPs). The median video length was 40 s (5–277 s), with >25 million cumulative views and a median of 446,400 views per video. The average DISCERN score was 29.0 ± 5.7, with HCPs providing higher-quality videos than non-HCPs (30.8 vs. 5.5, p < 0.05). HCPs also had more reliable videos (21.2 vs. 18.1, p < 0.05). Overall quality levels were mostly poor or very poor (97.4%), with none being good or excellent. Most HCP videos were poor (63.2%), whilst many non-HCP videos were very poor (61.0%). The most viewed video had 2,800,000 views but scored a 31 on the DISCERN tool and one on the completeness assessment. The highest DISCERN score had 11,700 views. HCP videos better defined the disease and were more complete (p < 0.05). Most videos discussed self-assessment but were lacking in definitions, risk factors, symptoms, evaluation, management, and outcomes. Most of TikTok’s testicular cancer information lacks quality and completeness, whilst higher-quality videos have limited reach. Full article

Background: Prostate cancer is a malignant neoplasm of the male genitourinary system with the highest incidence worldwide. Susceptibility genes related to aggressiveness and prognosis, such as BRCA1/2, ATM, PTEN, have been identified. Currently, reports related to germline mutations in patients with prostate cancer in Latin American populations are very limited or absent. In the Mexican population, reports are also limited, especially in the context of metastatic prostate cancer. Determining the prevalence of these mutations is relevant to predict the potential aggressiveness of tumors and allow the use of targeted therapies, such as PARPi inhibitors. Objective: Determine the prevalence of germline mutations in patients with metastatic prostate cancer and establish their clinical characteristics at diagnosis. Material and Methods: Sixty-nine patients with metastatic PCa underwent testing and genetic analysis using the Comprehensive Multi-Cancer Hereditary Cancer Panel. The prevalence of germline mutations was assessed, and the cohort was divided into two groups for the evaluation and analysis of clinical characteristics between the mutated and non-mutated populations. Results: We identified mutations in 15 out of 69 patients (21.73%), while 54 patients (78.26%) had no mutations. Pathogenic mutations were observed in 15.9% of patients, Variants of Uncertain Significance (VUS) in 34.78%, and 5.79% had both. The most frequent mutations included ATM (11.54%), BRCA1 (11.54%), BRCA2 (7.69%), FANCA (7.69%), and FANCM (7.69%). No statistically significant differences were found in PSA levels, age at diagnosis, and resistance to castration between the two groups. Conclusions: Our study unveiled a mutation rate of 21.73%, marked by a significant prevalence of ATM, FANCA, FANCM, and Variants of Uncertain Significance (VUS). This pattern deviates from findings in other series, underscoring the necessity for improved access to clinical genetic testing in our population. Full article

In recent years, 3D cell culture systems have emerged as sophisticated in vitro models, providing valuable insights into human physiology and diseases. The transition from traditional 2D to advanced 3D cultures has introduced novel obstacles, complicating the characterization and analysis of these models. While the lactate dehydrogenase (LDH) activity assay has long been a standard readout for viability and cytotoxicity assessments in 2D cultures, its applicability in long-term 3D cultures is hindered by inappropriate normalization and low LDH stability over time. In response to these challenges, we propose an optimization of LDH assays, including a crucial normalization step based on total protein quantification and a storage method using an LDH preservation buffer. We applied it to compare unexposed cerebral organoids with organoids exposed to a toxic dose of valproic acid, and showed efficient normalization of cellular viability as well as enhanced LDH stability within the buffer. Importantly, normalized LDH activity results obtained were independent of organoid dimension and cell density. This refined LDH assay, tailored to address 3D culture constraints, allows for the transposition of this routine test from 2D to 3D cultures. Full article

If the matrix function $f\left(At\right)$ posses the properties of $f\left(At\right)=g\left(f(\left(\frac{t}{k}A\right)\right)$, then the recurrence formula $f_{i-1}=g\left(f_i\right),i=N,N-1,\cdots ,1,f\left(tA\right)=f_0$, can be established. Here, $f_N=f\left(A_N\right)={\displaystyle \sum _{j=0}^m}{a}_j{A}_N^j,A_N=\frac{t}{k^N}A$. This provides an algorithm for computing the matrix function $f\left(At\right)$. By specifying the calculation accuracy p, a method is presented to determine m and N in a way that minimizes the time of the above algorithm, thus providing a fast algorithm for $f\left(At\right)$. It is important to note that m only depends on the calculation accuracy p and is independent of the matrix A and t. Therefore, $f\left(A_N\right)$ has a fixed calculation format that is easily computed. On the other hand, N depends not only on A, but also on t. This provides a means to select t such that N is equal to 0, a property of significance. In summary, the algorithm proposed in this article enables users to establish a desired level of accuracy and then utilize it to select the appropriate values for m and N to minimize computation time. This approach ensures that both accuracy and efficiency are addressed concurrently. We develop a general algorithm, then apply it to the exponential, trigonometric, and logarithmic matrix functions, and compare the performance with that of the internal system functions of Mathematica and Pade approximation. In the last section, an example is provided to illustrate the rapid computation of numerical solutions for linear differential equations. Full article

The study of salivary amino acid profiles has attracted the attention of researchers, since amino acids are actively involved in most metabolic processes, including breast cancer. In this study, we analyzed the amino acid profile of saliva in a sample including all molecular biological subtypes of breast cancer to obtain a more complete picture and evaluate the potential utility of individual amino acids or their combinations for diagnostic purposes. This study included 116 patients with breast cancer, 24 patients with benign breast disease, and 25 healthy controls. From all patients, strictly before the start of treatment, saliva samples were collected, and the quantitative content of 26 amino acids was determined. Statistically significant differences between the three groups are shown in the content of Asp, Gly, Leu + Ile, Orn, Phe, Pro, Thr, and Tyr. To differentiate the three groups from each other, a decision tree was built. To construct it, we selected those amino acids for which the change in concentrations in the subgroups was multidirectional (GABA, Hyl, Arg, His, Pro, and Car). For the first time, it is shown that the amino acid profile of saliva depends on the molecular biological subtype of breast cancer. The most significant differences are shown for the luminal B HER2-positive and TNBC subgroups. In our opinion, it is critically important to consider the molecular biological subtype of breast cancer when searching for potential diagnostic markers. Full article

Ice accumulation on lift-generating surfaces, such as rotor blades or wings, degrades aerodynamic performance and increases various risks. Active measures to counteract surface icing are energy-consuming and should be replaced by passive anti-icing surfaces. Two major categories of surface treatments—coating and structuring—already show promising results in the laboratory, but none fulfill the current industry requirements for performance and durability. In this paper, we show how femtosecond laser structuring of stainless steel (1.4301) combined with a hydrocarbon surface treatment or a vacuum treatment leads to superhydrophobic properties. The anti-ice performance was investigated in an icing wind tunnel under glaze ice conditions. Therefore, flexible steel foils were laser-structured, wettability treated and attached to NACA 0012 air foil sections. In the icing wind tunnel, hydrocarbon treated surfaces showed a 50 s ice build-up delay on the leading edge as well as a smoother ice surface compared to the reference. To demonstrate the erosion resistance of these surfaces, long-term field tests on a small-scale wind turbine were performed under alpine operating conditions. The results showed only minor erosion wear of micro- and nano-structures after a period of six winter months. Full article

We report the fabrication and characterisation of near-IR reflecting films and coatings based on shear-assembled crystalline ensembles of polymer composite microspheres, also known as “polymer opals”. Extension of the emulsion polymerisation techniques for synthesis of tractable larger core-interlayer-shell (CIS) particles, of up to half a micron diameter, facilitates the engineering and processing of thin-film synthetic opals, with a tunable photonic stopband spanning an extended spectral range of λ ≈ 700–1600 nm. Samples exhibit strong “scattering cone” interactions, with considerable angular dependence and angle tuning possible, as measured with a goniometric technique. These intense optical resonances in the near-IR, particularly within the important region around λ ~ 800 nm, combined with an appreciable translucency within the visible light spectrum, is indicative of the potential applications in coatings technologies and solar cells. Full article

This study aimed to investigate the impact of intradermal (ID) and intramuscular (IM) vaccination with a porcine reproductive and respiratory syndrome virus (PRRSV)-modified live vaccine (MLV) in piglets on serum copeptin levels and hematological profile. This study included 104 suckling piglets (2 weeks of age) from a commercial farrow-to-finish pig farm suffering from positive unstable PRRSV status. Animals were assigned to four groups, with two replicates (13 piglets/group/replicate); group A: IM vaccination with a PRRSV MLV vaccine, group B: ID vaccination with the same vaccine, group C: ID of Diluvac Forte, and group D: IM of Diluvac Forte. Blood samples were collected from the same three pigs/group/replicate at 4, 7, and 10 weeks of age. Blood samples were used for the performance of the complete blood count, and they were also examined by PCR for PRRSV and by ELISA for copeptin. No significant differences in serum copeptin levels and the number of blood cell counts (packed cell volume—PCV, numbers of white blood cells—WBCs, and platelets number—PLTs) were noticed in the same group over time and among groups. In conclusion, it seems that the vaccination against PRRSV does not affect the levels of the released copeptin. Based on our results, the measurement of serum copeptin could not be proposed as a potential stress biomarker in pigs. Full article

Hyperpigmentation skin disorders are marked by an abnormal accumulation or export of melanin pigment synthesized within melanocytes and pose a significant aesthetic concern. The search for novel natural compounds that exhibit pharmacological potential for treating pigmentation disorders is growing. In this study, kahweol (KW) and cafestol (CFS), two structural analogs of coffee diterpenes, were evaluated and compared for their effects on melanogenesis using B16F10 mouse melanoma cells and primary human melanocytes derived from Asian and African American skin. To the best of our knowledge, there are no reports of the effects of KW and CFS on melanogenesis yet. We first screened nontoxic concentrations of both compounds using an MTS assay after 72 h incubations and subsequently tested their effects on melanin synthesis and export. Cellular tyrosinase activity and cell-free mushroom tyrosinase activity were assayed to study the mechanisms of melanogenesis suppression. Human melanocytes from a moderately pigmented donor (HEMn-MP cells) and from a darkly pigmented donor (HEMn-DP cells) were next examined, and effects on cellular viability, melanin content, cellular tyrosinase activity, and melanin export (quantitated via dendricity) were similarly examined for both compounds. Our results show that KW and CFS did not significantly affect intracellular melanin content but suppressed extracellular melanin in B16F10 cells and dendritic parameters in human melanocytes, indicating their unique capacity to target extracellular melanogenesis and melanin export. Although KW showed a greater extracellular melanogenesis inhibitory capacity in B16F10 cells, in both primary melanocyte cells, CFS emerged as a potent inhibitor of melanin export compared to KW. Together, these results reveal novel modes of action of both compounds and indicate a promise to use CFS as a novel candidate for treating hyperpigmentation disorders of the human skin for clinical and cosmetic use. Additional research is necessary to shed light on the molecular pathways and the efficacy of melanogenesis inhibition by CFS in 3D human skin equivalents and in vivo studies. Full article

Iatrogenic amyloidosis results from medical therapeutic interventions, leading to the misfolding and aggregation of proteins into amyloid fibrils or to their direct deposition in different tissues. This review aims to provide a comprehensive overview of the iatrogenic amyloidosis pathology, underlying the possible molecular mechanisms, associated pathological manifestations, and clinical implications within modern medicine. By conducting a systematic analysis of the current literature, this paper highlights the diverse instances of iatrogenic amyloidosis triggered by medical procedures such as dialysis, organ and tissue transplantation, and therapeutic drugs. Exploring the intricate molecular pathways and contributing factors involved in protein misfolding and amyloidogenesis, and uncovering the pathological consequences observed in various tissues and organs, allows us to establish appropriate nomenclature and to gain a more profound understanding of the condition, working towards improved medical interventions and treatments. Full article

Three LED drivers which can be used for illumination, but whose main task is the transmission of information (data) via the light of the LEDs, are explored in this paper. The converter circuits need no capacitors for the energy transformation and avoid an inrush current. The lack of necessity of electrolytic capacitors reduces cost and space. Dimming the illumination is also easy to achieve. The control concept of the converters and the generation of pulsing of the LEDs for transmitting the information (data) are explained. The converters can also be expanded to more stages to drive more LEDs with different types of information. All three converters are explained in detail; all presented circuits are built up and simulated with LTSpice. Several data transmission concepts are applied and demonstrated through simulations. Full article

RAGE (receptor for advanced glycation end-products) represents a class of multi-ligand pattern recognition receptors highly expressed in the vertebrate lung. Our previous work demonstrated unique patterns of RAGE expression in the developing murine lung and regulation by key transcription factors including NKX2.1 and FoxA2. The current investigation employed conditional lung-specific upregulation via a TetOn transgenic mouse model (RAGE TG) and nontransgenic controls. RAGE expression was induced in RAGE TG mice throughout gestation (embryonic day, E0-E18.5) or from E15.5-E18.5 and compared to age-matched controls. High-resolution respirometry was used to assess mitochondrial respiration and context was provided by quantifying ATP and reactive oxygen species (ROS) generation. Lung lysates were also screened by immunoblotting for MAPK/PI3K signaling intermediates. RAGE upregulation increased mitochondrial oxygen consumption in the E0-E18.5 and E15.5-E18.5 groups compared to controls. RAGE TG mice also had increased ATP concentrations, which persisted even after controlling for oxygen consumption. In contrast, ROS generation was diminished in RAGE TG animals compared to controls. Lastly, in both RAGE TG groups, pERK and pp38 were significantly decreased, whereas pAKT was significantly elevated, suggesting that RAGE signaling is likely perpetuated via pAKT pathways. Together, these data demonstrate that despite lung hypoplasia in RAGE TG mice, the remaining tissue experiences a favorable shift in mitochondrial bioenergetics without excessive redox assault and a preference for AKT signaling over ERK or p38. Full article

A 74-year-old woman in good general health presented with a 5-year history of progressive hair loss over several years, interpreted as female androgenetic alopecia (AGA), and was treated with topical 5% Minoxidil without improvement. The patient’s relevant medical history revealed infiltrating, triple-negative apocrine carcinoma of the right breast four years before, treated by quadrantectomy, radiation, lymphadenectomy and chemotherapy, with no recurrence at the last follow-up. On examination, there was an asymptomatic 15 × 15 cm firm and whitish area of scarring alopecia on the central scalp. Dermoscopy revealed multiple arborizing vessels and many telangiectasia. The clinical considerations included mainly cutaneous metastasis of breast carcinoma (alopecia neoplastica), pseudopelade of Broque and morpheaform basal cell carcinoma (BCC). A histopathologic examination revealed characteristic changes of morpheaform BCC with basaloid islands and cords of atypical basaloid cells diffusely infiltrating the dermis, embedded in a sclerotic and hypervascularized stroma. Secondary alopecia neoplastica due to morpheaform BCC on the scalp is an exceedingly rare entity, possessing subtle clinical features that may mimic both scarring and non-scarring alopecia. Delayed recognition may contribute to aggressive behavior and extensive local destruction. Treatment with hedgehog inhibitors in locally advanced BCC of the scalp, both in adjuvant and neoadjuvant modalities, is promising. Full article

The conservation of endemic plant species has come into the global spotlight, not only because of their contribution to biodiversity but also their vulnerability and high extinction risk due to environmental and anthropogenic threats. Based on these developments, it is now essential to monitor and protect these species by applying integrated conservation strategies, especially in view of climate change, which is one of the most severe threats to plants. Genetic and genomic tools provide new potential in assessing and quantifying genetic diversity and thus can be utilized to devise conservation strategies and contribute to biodiversity conservation efforts. Greece comprises a plant biodiversity hotspot in the Mediterranean Basin with a wide variety of rare, threatened, and endemic plant taxa. In this review, we examine several cases where a broad spectrum of genetic tools has been utilized so far in the diversity assessment and conservation management of Greek Endemic Plants (GEPs). Following an extensive database search, we have identified and included in our final data collection 19 studies concerning 32 GEPs for which molecular markers have been used for the determination of population genetic structure and diversity assessment, while at the same time, the research outcomes have been taken into consideration for conservation management. The limited application of genetic and genomic tools in GEP management is demonstrated, while the significance of implementing a comprehensive conservation strategy that will integrate genetic analyses and the data derived therein is also highlighted. Full article

Today, many individuals read the daily news from social media platforms. Research has shown that news with negative valence might influence the well-being of individuals. Existing research that examined the impact of headlines on individuals’ well-being has primarily focused on examining the positive or negative polarity of words used in the headlines. In the present study, we adopt a different approach and ask participants to categorize the headlines themselves based on the emotions they experienced while reading them and how their choice impacts their well-being. A total of 306 participants were presented with 40 headlines from main news sites that were considered popular based on the number of public reactions. Participants had to rate their emotional experience of the headlines following five emotional states (i.e., happiness, anger, sadness, fear, and interest). Emotion regulation strategies and resilience were also measured. In line with our hypotheses, we found that participants reported experiencing negative emotions more intensively while reading the headlines. Emotion regulation was not found to influence the emotional states of individuals, whereas resilience did. These findings highlight that individuals can experience heightened emotions without reading the entire news story. This effect was observed regardless of the headline’s emotional valence (i.e., positive, negative, or neutral). Furthermore, our study highlights the critical role of interest as a factor in news consumption. Interest significantly affects individuals’ engagement and reactions to headlines, regardless of valence. The findings underscore the complex interplay between headline content and reader engagement and stress the need for further research into how headlines are presented to protect individuals from potential emotional costs. Full article

Multimedia resources, such as instructional videos, are currently enjoying a certain popularity in the training programs for medical and dental students. The major challenge is to create such resources with quality content that is approved by students. In order to answer this challenge, it is imperative to find out which features of instructional videos are considered to be necessary and useful by students, thus being able to excite them, to hold their attention, and to stimulate them in learning with pleasure. Aim: We investigated the opinions of a sample of 551 students from four medical universities in Romania, in order to identify the students’ preferred characteristics in instructional videos, both globally and comparatively on genders and age groups and also according to their general preferences for using internet services. Material and methods: We used univariate (hypothesis testing) and multivariate (two-step clustering) data analysis techniques and revealed three clusters of students, primarily determined by their perceptions of the visual appearance of the instructional videos. Results: The structure of the clusters by gender and age group was relatively similar, but we recorded differences associated with the students’ expressed preferences for certain internet services compared to others. The first identified cluster (35.4% of the cases) contains students who prefer instructional videos to contain images used only for aesthetic purposes and to fill the gaps; they use internet services mainly for communication. The second cluster of students (34.8%) prefers videos designed as practical lessons, using explanatory drawings and diagrams drawn at the same time as the explanations; they also use internet services mainly for communication. The last cluster of students (29.8%) prefer videos designed as PowerPoint presentations, with animated pictures, diagrams, and drawings; they are slightly younger than the others and use internet services mainly for information and communication, but also for domestic facilities. Conclusions: The students’ preferences for certain features of instructional videos depend not only on gender and age but are also related to their developmental background and general opinions about modern technologies. Full article

The complement cascade is a vital system in the human body’s defense against pathogens. During the natural aging process, it has been observed that this system is imperative for ensuring the integrity and homeostasis of the retina. While this system is critical for proper host defense and retinal integrity, it has also been found that dysregulation of this system may lead to certain retinal pathologies, including geographic atrophy and diabetic retinopathy. Targeting components of the complement system for retinal diseases has been an area of interest, and in vivo, ex vivo, and clinical trials have been conducted in this area. Following clinical trials, medications targeting the complement system for retinal disease have also become available. In this manuscript, we discuss the pathophysiology of complement dysfunction in the retina and specific pathologies. We then describe the results of cellular, animal, and clinical studies targeting the complement system for retinal diseases. We then provide an overview of complement inhibitors that have been approved by the Food and Drug Administration (FDA) for geographic atrophy. The complement system in retinal diseases continues to serve as an emerging therapeutic target, and further research in this field will provide additional insights into the mechanisms and considerations for treatment of retinal pathologies. Full article

Background and Objectives: Medical and public recognition of “long-COVID or post-COVID syndrome”, as well as its impact on the quality of life (QoL), is required to better address the disease burden. Objectives: We aimed to describe the persistence of COVID-19 symptoms and QoL among patients at three and twelve months after their discharge from the hospital. Materials and Methods: We conducted an observational, prospective, and longitudinal analytic study from September 2021 to April 2022. To measure QoL, we used a validated version of the 36-item Short-Form Health Survey (SF-36). Results: We included 68 patients in the study. A total of 54 (79.4%) patients reported at least one persistent symptom at three months vs. 52 (76.4%) at twelve months (p = 0.804). Some persistent symptoms (myalgia, alopecia, and cough) decreased significantly at twelve months (50% vs. 30.9%, 29.4% vs. 13.2%, and 23.5% vs. 7.4%; respectively, p = 0.007); in contrast, other persistent symptoms (sleep–wake and memory disorders) were more frequent (5.9% vs. 32.4% and 4.4% vs. 20.6%; respectively, p = ≤0.001). Regarding QoL, a statistically significant improvement was observed in some scores over time, p = ≤0.037. At twelve months, dyspnea, myalgia, and depression were risk factors associated with a poor physical component summary (PCS), p = ≤0.027, whereas anxiety, depression, and fatigue were associated with a poor mental component summary (MCS), p = ≤0.015. Conclusion: As the proportion of persistent symptoms at twelve months is high, we suggest that patients must continue under long-term follow up to reclassify, diagnose, and treat new onset symptoms/diseases. Full article

Saffron cultivation is important in global agriculture and is mainly flourishing in Mediterranean climates. Although it originated in Asia Minor, it is widely grown in regions such as Iran, India, Spain, Morocco, Greece, and Italy. Labour-intensive harvesting, mainly by hand, characterises its production and offers substantial employment opportunities in cultivating areas. However, traditional saffron-producing countries such as Spain, Italy, and Greece have witnessed declining production due to labour demands and competition from low-wage countries. Mechanization remains unfeasible due to the delicate nature of the plant. To revitalise saffron cultivation, efforts have been focused on reducing labour costs, improving productivity, and improving quality through innovative techniques, such as soilless crops. In this study, the productivity and quality of saffron was evaluated in a soilless culture and three fertigation doses were evaluated: a control, consisting of Sonneveld and Voogt’s standard nutrient solution, and two treatments with two supplemented solutions, 30% K and 30% Ca. The results indicated that the solution with 30% K obtained higher corm productivity, as well as better quality saffron, as all the products of this treatment were included in Category I according to the ISO 3632 standard, while the quality of saffron grown with the control solution was lower. Full article

The application of pesticides using unmanned aerial vehicles (UAVs) has grown, but there is a lack of information to support more efficient applications. Using a DJI AGRAS-MG-1P octocopter equipped with different spray tips, this study sought to explore spray deposition (leaves and fruit) and efficacy of chlorpyrifos on control of coffee berry borer at different spray volumes and flight heights. The study was conducted in an Arabica coffee plantation. The study consisted of eight treatments and four replications in a 2 × 2 × 2 factorial scheme: two flight heights (1.5 and 3.0 m), two spray tips (hollow cone and flat fan), and two spray volumes (10 and 15 L ha⁻¹). Deposition was assessed by detecting a tracer in the coffee leaves and fruit using spectrophotometry. The coffee berry borer-control efficacy trial was conducted in two areas by evaluating the percentage of damaged fruit 60 days after two insecticide applications. The flight height of 1.5 m promoted higher spray deposition on leaves and fruit and a lower incidence of damaged fruit. Flat fan spray tips resulted in higher spray deposition on the leaves, not interfering with the deposition on fruit or the coffee berry borer control. Increasing the spray volume from 10 to 15 L ha⁻¹ did not increase spray deposition on coffee leaves and fruit. Chlorpyrifos applied via UAVs reduced the incidence of coffee berry borer. Full article