Mechanical threshing can cause damage to oats, and the nutritional quality of the damaged kernels easily changes during storage. The current research on oats with regard to threshing damage and nutritional quality falls into two separate research areas, but they are inextricably linked. It is necessary to combine these two types of studies. In order to explore the damage mechanism of oat threshing and its effect on the nutritional components, a three-dimensional model of oat–nail-tooth impact was established, and a finite element analysis of the dynamic impact process was performed using LS-DYNA. The changes in the von Mises stress, contact force, energy, critical velocity, and damage characteristics during impact on different surfaces of the kernel were analyzed. A threshing test was carried out, and the kernels were classified according to their different damage types. The differences in the nutrient compositions and contents of oats with different damage types were analyzed by means of near-infrared spectroscopy and physicochemical testing. The results showed that the von Mises stress and contact force tended to first increase and then decrease during impact. When the impact velocity was 12 m/s, for the top, bottom, front, back, and left sides of the oat impacted by the nail tooth, the maximum von Mises stresses were 10.05, 10.46, 8.60, 9.28, and 8.49 MPa, respectively. The maximum contact forces were 25.09, 18.57, 34.29, 38.37, and 35.19 N, respectively. The critical velocities of impact damage were 13.38, 13.10, 13.40, 14.64, and 16.00 m/s, respectively. The threshing damage could be divided into four typical types: transverse fracture, bottom breakage, side fracture, and back crack. The chemical compositions of oat kernels with different damage types were basically the same, but their nutrient contents were different. These results provide a theoretical basis for optimizing oat threshing devices, reducing threshing damage, and improving oat quality. Full article

This article describes the synthesis of hydroxyapatite (HAp) flakes through a microwave-assisted hydrothermal method. These flakes suggest possible applications as a substrate for depositing titanium dioxide (TiO₂) films using chemical vapor deposition with metal–organic precursors (MOCVD). The results reveal the formation of crystalline hydroxyapatite characterized by a uniform morphology. Additionally, we demonstrated the successful deposition of TiO₂ coatings on the hydroxyapatite flakes, resulting in a distinctive faceted prism morphology. Our findings affirm the effective synthesis of the HAp/TiO₂ composite material. To further explore the material’s practical applications, we recommend assessing the photocatalytic activity of these composite membranes in future research. Full article

COVID-19 is a systemic disease caused by the etiologic agent SARS-CoV-2, first reported in Hubei Province in Wuhan, China, in late 2019. The SARS-CoV-2 virus has evolved over time with distinct transmissibility subvariants from ancestral lineages. The clinical manifestations of the disease vary according to their severity and can range from asymptomatic to severe. Due to the rapid evolution to a pandemic, epidemiological studies have become essential to understand and effectively combat COVID-19, as the incidence and mortality of this disease vary between territories and populations. This study correlated epidemiological data on the incidence and mortality of COVID-19 with frequencies of important SNPs in GWAS studies associated with the susceptibility and mortality of this disease in different populations. Our results indicated significant correlations for 11 genetic variants (rs117169628, rs2547438, rs2271616, rs12610495, rs12046291, rs35705950, rs2176724, rs10774671, rs1073165, rs4804803 and rs7528026). Of these 11 variants, 7 (rs12046291, rs117169628, rs1073165, rs2547438, rs2271616, rs12610495 and rs35705950) were positively correlated with the incidence rate, these variants were more frequent in EUR populations, suggesting that this population is more susceptible to COVID-19. The rs2176724 variant was inversely related to incidence rates; therefore, the higher the frequency of the allele is, the lower the incidence rate. This variant was more frequent in the AFR population, which suggests a protective factor against SARS-CoV-2 infection in this population. The variants rs10774671, rs4804803, and rs7528026 showed a significant relationship with mortality rates. SNPs rs10774671 and rs4804803 were inversely related to mortality rates and are more frequently present in the AFR population. The rs7528026 variant, which is more frequent in the AMR population, was positively related to mortality rates. The study has the potential to identify and correlate the genetic profile with epidemiological data, identify populations that are more susceptible to severe forms of COVID-19, and relate them to incidence and mortality. Full article

Serotonin (5-hydroxytryptamine, 5-HT) is a neurotransmitter regulating numerous physiological functions, and its dysregulation is a crucial component of the pathological processes of schizophrenia, depression, migraines, and obesity. 5-HT interacts with 14 different receptors, of which 5-HT_1A-1FRs, 5-HT_2A-CRs, and 5-HT_4-7Rs are G protein-coupled receptors (GPCRs), while 5-HT₃R is a ligand-gated ion channel. Over the years, selective orthosteric ligands have been identified for almost all serotonin receptors, yielding several clinically relevant drugs. However, the high degree of homology between 5-HTRs and other GPCRs means that orthosteric ligands can have severe side effects. Thus, there has recently been increased interest in developing safer ligands of GPCRs, which bind to less conserved, more specific sites, distinct from that of the receptor’s natural ligand. The present review describes the identification of allosteric ligands of serotonin receptors, which are largely natural compounds (oleamide, cannabidiol, THC, and aporphine alkaloids), complemented by synthetic modulators developed in large part for the 5-HT_2C receptor. The latter are positive allosteric modulators sought after for their potential as drugs preferable over the orthosteric agonists as antiobesity agents for their potentially safer profile. When available, details on the interactions between the ligand and allosteric binding site will be provided. An outlook on future research in the field will also be provided. Full article

To reduce the iron loss and torque ripple of an external rotor consequent pole (ERCP) motor used in an electric vehicle air-conditioning compressor, the magnetic pole structure of the motor was improved, and an unequal piecewise consequent pole (CP) structure was designed. The performance of the motor is optimized by reducing the harmonic content in the air gap flux density and reducing the iron saturation degree of the motor. The designed CP structure can significantly reduce the iron loss and torque ripple of the motor. Based on the Taguchi method, the optimal size parameters of the unequal piecewise CP structure are determined, and the final optimization design scheme is obtained. The results of finite element simulation and high-precision iron loss model show the following: compared with the original motor, the iron loss and torque ripple of the motor with the final optimized design scheme are significantly reduced. Full article

Pumps-as-Turbines (PATs) are increasingly used in micro-hydropower applications due to their cost competitiveness that is brought about by lower acquisition, design, operation, and maintenance costs. Despite these, limited research exists that investigates PAT failures. Notably, there is a literature gap concerning cavitation in PATs. As such, this study proposes an improvement to the deviation from the normal distribution (DND) technique to facilitate application in PAT cavitation detection. Probability density functions of vibration signals collected during operation at design speed and various cavitation states are developed and the DND computed using two approaches, i.e., the use of baseline data and the original method, for comparison purposes. Normal probability plots are presented to depict suitability of the two approaches in quantifying the DND. Results show higher deviation when using baseline data, hence, improved detection capabilities with amplification of the slope of the trend line under cavitating conditions when using the proposed DND approach. The proposed method also allows for establishing clear alarm limits for the condition monitoring of PATs in practice. Moreover, the proposed method is validated by application at various PAT operating speeds and cavitation states. The proposed method is found to be responsive, reliable, and independent from operating speed. Full article

Total hip arthroplasty (THA) has revolutionized patients’ lives with hip osteoarthritis. However, the increasing prevalence of THA in individuals with prior lumbar arthrodesis (LA) poses unique challenges. This review delves into the biomechanical alterations, complications, and surgical strategies specific to this patient subset, highlighting the need for tailored preoperative assessments and planning. Due to altered pelvic and spinal biomechanics, patients with LA undergoing THA face a higher risk of dislocation and revision. The complex interplay between spinal and hip biomechanics underscores the need for meticulous preoperative planning. Comprehensive clinical examination and radiographic evaluation are vital for understanding patient-specific challenges. Various radiographic techniques, including computed tomography (CT)/X-ray matching and standing/seated studies, provide insights into postural changes affecting pelvic and spinal alignment. Complications following THA in patients with LA highlight the necessity for personalized surgical strategies. Careful consideration of implant selection, the surgical approach, and component positioning are essential to prevent complications. In summary, THA in patients with prior LA demands individualized preoperative assessments and planning. This approach is crucial to optimize outcomes and mitigate the heightened risks of complications, underlining the importance of tailored surgical strategies. Full article

Background: Pediatric colorectal specialists care for patients with a variety of defecation disorders. Anorectal (AR) manometry testing is a valuable tool in the diagnosis and management of these children. This paper provides a summary of AR manometry techniques and applications as well as a review of AR manometry findings in pediatric patients with severe defecation disorders referred to a pediatric colorectal center. This is the first study describing multi-year experience using a portable AR manometry device in pediatric patients. Methods: An electronic medical record review was performed (1/2018 to 12/2023) of pediatric patients with defecation disorders who had AR manometry testing. Demographics, diagnostic findings, and outcomes are described. Key Results: A total of 297 unique patients (56.9% male, n = 169) had AR manometry testing. Of these, 72% (n = 188) had dyssynergic defecation patterns, of which 67.6% (n = 127) had fecal soiling prior to treatment. Pelvic rehabilitation (PR) was administered to 35.4% (n = 105) of all patients. A total of 79.5% (n = 58) of the 73 patients that had fecal soiling at initial presentation and completed PR with physical therapy and a bowel management program were continent after therapy. AR manometry was well tolerated, with no major complications. Conclusions: AR manometry is a simple test that can help guide the management of pediatric colorectal surgical patients with defecation disorders. As a secondary finding, PR is a useful treatment for patients with dyssynergic stooling. Full article

Axiidea housed in the collection of the Museu de Oceanografia Prof. Petrônio Alves Coelho, Universidade Federal de Pernambuco (MOUFPE) were studied. This collection contains 66% of the total diversity of axiideans recorded from the continental shelf of the Brazilian coast. The species are listed by family and genus as follows: Axiidea: Axiidae (Axiopsis, Axiorygma, Calaxius, Coralaxius, Manaxius, Paraxiopsis), Callianassidae (Cheramoides), Callichiridae (Callichirus, Corallianassa, Lepidophthalmus, Neocallichirus), Ctenochelidae (Ctenocheles, Ctenocheloides, Dawsonius, Gourretia), Micheleidae (Marcusiaxius, Meticonaxius). Details on the biology and taxonomy of some of these species are given. In addition, certain poorly documented species are now better understood. Full article

Longan (Dimcarpus longan Lour.) is a kind of traditional fruit used as a medicine and a food. Fresh longan is primarily consumed as a fruit, whereas dried longan is commonly employed for medicinal purposes. The differences in the immunomodulatory activities and mechanisms of polysaccharides between dried and fresh longan remain unclear. The present study comparatively analyzed the mechanisms of macrophage activation induced by polysaccharides from dried (LPG) and fresh longan (LPX). The results revealed that LPG and LPX differentially promoted macrophage phagocytosis and the secretion of NO, TNF-α, and IL-6. RNA-seq analysis revealed that LPG and LPX differentially affected gene expression in macrophages. The LPG treatment identified Tnf and chemokine-related genes as core genes, while myd88 and interferon-related genes were the core genes affected by LPX. A comprehensive analysis of the differentially expressed genes showed that LPG initiated macrophage activation primarily through the TLR2/4-mediated TRAM/TRAF6 and CLR-mediated Src/Raf1 NF-κB signaling pathways. LPX initiated macrophage activation predominantly via the CLR-mediated Bcl10/MALT1 and NLR-mediated Rip2/TAK1 MAPK and NF-κB signaling pathways. Interestingly, the non-classical NF-κB signaling pathway was activated by polysaccharides in both dried and fresh longan to elicit a slow, mild immune response. LPG tends to promote immune cell migration to engage in the immune response, while LPX facilitates antigen presentation to promote T cell activation. These findings contribute insights into the mechanisms underlying the differences in bioactivity between dried and fresh longan and their potential applications in immune-enhancing strategies and functional-food development. Full article

The current paper concentrates on discovering the exact solutions of the singular time-fractional Boussinesq equation and coupled time-fractional Boussinesq equation by presenting a new technique known as the double Sumudu–generalized Laplace and Adomian decomposition method. Here, two main theorems are addressed that are very useful in this work. Moreover, the mentioned method is effective in solving several problems. Some examples are presented to check the precision and symmetry of the technique. The outcomes show that the proposed technique is precise and gives better solutions as compared to existing methods in the literature. Full article

Coalbed methane (CBM) is the only unconventional gas in Poland with estimated recoverable resources. The prospects for developing deep CBM have been explored in recent years by drilling deep exploration wells within the depocenter of the Upper Silesian Coal Basin. The purpose of this study is to analyze the occurrence and potential for CBM extraction in this area of the basin, which can be considered prospective due to the confirmed presence of significant amounts of gas and thick coal seams at depths > 1500 m. The study examined the vertical and horizontal variability of the gas content in the studied area, the coal rank in the seams, thermal conditions, and coal reservoir parameters. The gas content in the seams, reaching more than 18 m³/t coal^daf at a depth of 2840 m, and indicative estimated gas resources of 9 billion m³ were found. The high gas content is accompanied by positive thermal and coal rank anomalies. The permeability and methane saturation of the coal seams are low, and therefore, potential methane production may prove problematic. However, the development of CBM extraction technologies involving directional drilling with artificial fracturing may encourage gas production testing in the study area. Full article

Surface soil moisture (SSM) reflects the dry and wet states of soil. Microwave remote sensing technology can accurately obtain regional SSM in real time and effectively improve the level of agricultural drought monitoring, and it is of great significance for agricultural precision irrigation and smart agriculture construction. Based on Sentinel-1, Sentinel-2, and Landsat-8 images, the effect of vegetation was removed by the water cloud model (WCM), and SSM was retrieved and validated by a radial basis function (RBF) neural network model in bare soil and vegetated areas, respectively. The normalized difference vegetation index (NDVI) calculated by Landsat-8 (NDVI_Landsat-8) had a better effect on removing the influence the of vegetation layer than that of NDVI_Sentinel-2. The RBF network model, established in a bare area (R = 0.796; RMSE = 0.029 cm³/cm³), and the RBF neural network model, established in vegetated areas (R = 0.855; RMSE = 0.024 cm³/cm³), have better simulation effects on SSM than a linear SSM inversion model with single polarization. The introduction of surface parameters to the RBF neural network model can improve the accuracy of the model and realize the high-accuracy inversion of SSM in the study area. Full article

With more frequent and intense fires expected under future climate conditions, it is important to understand the mechanisms that control flammability in Australian forests. We followed a systematic review approach to determine which physical traits make eucalypts leaves more or less flammable. Specifically, we reviewed 20 studies that covered 35 eucalypt species across five countries and found that leaf water content, leaf area (LA), and specific leaf area (SLA) are the main drivers of leaf flammability. These traits are easy and straightforward to measure, while more laborious traits (e.g., volatile organic compounds and structural carbohydrates) are seldom measured and reported. Leaf flammability also varies with species, and, while the biochemistry plays a role in how leaves burn, it plays a minor role in fire behaviour at landscape scales. This review highlights the range of different protocols used to measure flammability and leaf water content, warranting caution when comparing traits and results between studies. As a result, we propose a standardised protocol to measure leaf water content and advocate for long-term measurements of leaf traits and flammability. This study not only contributes to the understanding of how and why eucalypt leaves burn but also encourages research into the relative importance of traits in influencing flammability and provides a guide for selecting traits that can be monitored using satellite images to inform fire management policies and strategies. Full article

Background: The incidence and mortality of pleural mesothelioma (PM) reflect the production and consumption of asbestos over time. However, despite the current global concern, these data remain to be known. Objective: Our aim was to carry out a descriptive analysis of PM cases and mortality from some Portuguese databases between 2014 and 2020. Methods: A retrospective observational study was carried out between 2014 and 2020. Data on the number of PM cases were provided by the Portuguese Cancer Registry, and data on mortality were from the Portuguese Death Certificate Information System. Results: Between 2014 and 2020, 315 cases of PM were reported, with 222 (70.5%) men. The average age of patients was 72.1, with the highest number of cases in patients aged >70 years (n = 198; 62.9%). The highest number of cases was reported in 2018 (n = 62; 19.7%). Regarding mortality, 169 deaths were reported, with 126 (74.6%) men and mostly in individuals aged >70 years (n = 109; 64.5%). It is estimated that around 520 years of potential life were lost. The highest number of deaths occurred in 2015 (n = 33; 19.5%). Conclusion: It is mandatory to reinforce the need for surveillance programs that allow us to gather real and reliable data and eliminate asbestos-related diseases. Full article

The development of next-generation sequencing (NGS) has enabled the discovery of cancer-specific driver gene alternations, making precision medicine possible. However, accurate genetic testing requires a sufficient amount of tumor cells in the specimen. The evaluation of tumor content ratio (TCR) from hematoxylin and eosin (H&E)-stained images has been found to vary between pathologists, making it an important challenge to obtain an accurate TCR. In this study, three pathologists exhaustively labeled all cells in 41 regions from 41 lung cancer cases as either tumor, non-tumor or indistinguishable, thus establishing a “gold standard” TCR. We then compared the accuracy of the TCR estimated by 13 pathologists based on visual assessment and the TCR calculated by an AI model that we have developed. It is a compact and fast model that follows a fully convolutional neural network architecture and produces cell detection maps which can be efficiently post-processed to obtain tumor and non-tumor cell counts from which TCR is calculated. Its raw cell detection accuracy is 92% while its classification accuracy is 84%. The results show that the error between the gold standard TCR and the AI calculation was significantly smaller than that between the gold standard TCR and the pathologist’s visual assessment ($p<0.05$). Additionally, the robustness of AI models across institutions is a key issue and we demonstrate that the variation in AI was smaller than that in the average of pathologists when evaluated by institution. These findings suggest that the accuracy of tumor cellularity assessments in clinical workflows is significantly improved by the introduction of robust AI models, leading to more efficient genetic testing and ultimately to better patient outcomes. Full article

Background: Neisseria meningitidis (meningococcus) is a Gram-negative bacterium that colonises the nasopharynx of about 10% of the healthy human population. Under certain conditions, it spreads into the body, causing infections with high morbidity and mortality rates. Although the capsule is the key virulence factor, unencapsulated strains have proved to possess significant clinical implications as well. Meningococcal meningitis is a primarily human infection, with limited animal models that are dependent on a variety of parameters such as bacterial virulence and mouse strain. In this study, we aimed to develop a murine Neisseria meningitidis meningitis model to be used in the study of various antimicrobial compounds. Method: We used a capsule-deficient Neisseria meningitidis strain that was thoroughly analysed through various methods. The bacterial strain was incubated for 48 h in brain–heart infusion (BHI) broth before being concentrated and injected intracisternally to bypass the blood–brain barrier in CD-1 mice. This prolonged incubation time was a key factor in increasing the virulence of the bacterial strain. A total of three more differently prepared inoculums were tested to further solidify the importance of the protocol (a 24-h incubated inoculum, a diluted inoculum, and an inactivated inoculum). Antibiotic treatment groups were also established. The clinical parameters and number of deaths were recorded over a period of 5 days, and comatose mice with no chance of recovery were euthanised. Results: The bacterial strain was confirmed to have no capsule but was found to harbour a total of 56 genes coding virulence factors, and its antibiotic susceptibility was established. Meningitis was confirmed through positive tissue culture and histological evaluation, where specific lesions were observed, such as perivascular sheaths with inflammatory infiltrate. In the treatment groups, survival rates were significantly higher (up to 81.25% in one of the treatment groups compared to 18.75% in the control group). Conclusion: We managed to successfully develop a cost-efficient murine (using simple CD-1 mice instead of expensive transgenic mice) meningococcal meningitis model using an unencapsulated strain with a novel method of preparation. Full article

The thermal properties, microstructure, and mechanical properties of Fe-18Mn-3Ti (wt%) were investigated, focusing on the effects of different heat-treatment processes. Results revealed that the 450 °C warm-rolling sample (450 WR) exhibited promising mechanical properties. Specifically, this sample displayed a yield strength of 988 MPa, an ultimate tensile strength of 1052 MPa, and total elongation of 15.49%. Consequently, a favorable strength-ductility balance was achieved. The strain-hardening ability surpassed that of the cold rolling sample (CR). Microstructure analysis indicated the simultaneous occurrence of dynamic equilibrium between grain deformation and re-crystallization because of the co-influence of thermal and strain in the warm rolling process. This desirable mechanical property was attributed to the presence of a multi-phase (α-martensite, austenite, and ε-martensite) and heterogeneous microstructure. The improvement of ultimate tensile strength was based on grain refinement, grain co-deformation, and the transformation-induced plasticity (TRIP) effect in the early stage of plastic deformation (stage Ⅰ). The improvement of ultimate elongation (TEL) was ascribed to the TRIP effect in the middle stage of plastic deformation (stage Ⅱ). Full article

The repurposing of previously clinically approved drugs as an alternative therapeutic approach to treating disease has gained significant attention in recent years. A multitude of studies have demonstrated various and successful therapeutic interventions with these drugs in a wide range of neoplastic diseases, including multiple myeloma, leukaemia, glioblastoma, and colon cancer. Drug repurposing has been widely encouraged due to the known efficacy, safety, and convenience of already established drugs, allowing the bypass of the long and difficult road of lead optimization and drug development. Repurposing drugs in cancer therapy is an exciting prospect due to the ability of these drugs to successfully target cancer-associated genes, often dysregulated in oncogenic signalling pathways, amongst which are the classical cancer signalling pathways; WNT (wingless-related integration type) and Hippo signalling. These pathways play a fundamental role in controlling organ size, tissue homeostasis, cell proliferation, and apoptosis, all hallmarks of cancer initiation and progression. Prolonged dysregulation of these pathways has been found to promote uncontrolled cellular growth and malignant transformation, contributing to carcinogenesis and ultimately leading to malignancy. However, the translation of cancer signalling pathways and potential targeted therapies in cancer treatment faces ongoing challenges due to the pleiotropic nature of cancer cells, contributing to resistance and an increased rate of incomplete remission in patients. This review provides analyses of a range of potential anti-cancer compounds in drug repurposing. It unravels the current understanding of the molecular rationale for repurposing these drugs and their potential for targeting key oncogenic signalling pathways. Full article

This study describes and explores the application of no-restraint policies in General Hospital Psychiatric Units (GHPUs) in Italy, a country pioneering in deinstitutionalization and psychiatric reform. The research aims to assess the organizational characteristics and effectiveness of no-restraint practices, contributing to the global discourse on humane psychiatric care. Following a purposive sampling approach, a nationwide descriptive study was conducted involving a detailed online survey distributed to 24 GHPUs actively engaged in or aspiring toward no-restraint practices. The survey, comprising 60 items across seven sections, gathered comprehensive data on the structural, organizational, and operational dimensions of the units, along with the prevalence and management of restraint episodes. Results reveal a significant commitment to no-restraint policies, with 14 GHPUs reporting zero restraint incidents in 2022. Despite variations in infrastructure and staffing, a common thread was the implementation of systematic procedures and risk management training aimed at reducing coercive practices. The study identified a correlation between the use of exclusive garden spaces and an increased incidence of restraints, suggesting nuanced factors influencing restraint practices. The findings underscore the viability and ethical alignment of no-restraint practices within psychiatric care, highlighting the crucial role of organizational protocols and training. This research adds empirical weight to the advocacy for restraint-free environments in mental health settings, signaling a paradigm shift toward more humane and rights-respecting psychiatric care. Full article

In order to explore the flexural behavior of a concrete sandwich panel under concentrated boundary conditions, based on Kirachhoff’s elastic thin plate theory in this paper, the geometric deformation, physical conditions, and equilibrium relationship of a sandwich panel are deduced by constructing the layered analysis model of the sandwich panel, the basic differential equation of the flexural deformation of the concrete sandwich thin plate is obtained, and the mathematical expression of the internal force and displacement under the boundary condition of concentrated support is given. Combined with an engineering example, the proposed calculation method is verified. The results show that, in the arrangement of reliable connectors for concrete sandwich panels, the concrete wythes bear the load while the contribution of the core layer to the bending capacity of the structure can be ignored. When subjected to a laterally distributed load, the sandwich panel mainly experiences out-of-plane bending deformation, and the bending normal stress in the concrete panel layer shows a linear non-uniform distribution along the thickness direction of the panel. The bending deformation performance and bearing efficiency of a concrete sandwich slab with the change in concentrated support position have significant effects, and the load transfer efficiency can be improved by optimizing the arrangement of supports. Except for small local areas near the supports, the bending stress distribution and deformation behavior of the concrete sandwich panel can be accurately analyzed by the calculation method established in this paper. Full article

The evolution of 3D printing has ushered in accessibility and cost-effectiveness, spanning various industries including biomedical engineering, education, and microfluidics. In biomedical engineering, it encompasses bioprinting tissues, producing prosthetics, porous metal orthopedic implants, and facilitating educational models. Hybrid Additive Manufacturing approaches and, more specifically, the integration of Fused Deposition Modeling (FDM) with bio-inkjet printing offers the advantages of improved accuracy, structural support, and controlled geometry, yet challenges persist in cell survival, interaction, and nutrient delivery within printed structures. The goal of this study was to develop and present a low-cost way to produce physical phantoms of human organs that could be used for research and training, bridging the gap between the use of highly detailed computational phantoms and real-life clinical applications. To this purpose, this study utilized anonymized clinical Computed Tomography (CT) data to create a liver physical model using the Creality Ender-3 printer. Polylactic Acid (PLA), Polyvinyl Alcohol (PVA), and light-bodied silicone (Polysiloxane) materials were employed for printing the liver including its veins and arteries. In brief, PLA was used to create a mold of a liver to be filled with biocompatible light-bodied silicone. Molds of the veins and arteries were printed using PVA and then inserted in the liver model to create empty channel. In addition, the PVA was then washed out by the final product using warm water. Despite minor imperfections due to the printer’s limitations, the final product imitates the computational model accurately enough. Precision adjustments in the design phase compensated for this variation. The proposed novel low-cost 3D printing methodology successfully produced an anatomically accurate liver physical model, presenting promising applications in medical education, research, and surgical planning. Notably, its implications extend to medical training, personalized medicine, and organ transplantation. The technology’s potential includes injection training for medical professionals, personalized anthropomorphic phantoms for radiation therapy, and the future prospect of creating functional living organs for organ transplantation, albeit requiring significant interdisciplinary collaboration and financial investment. This technique, while showcasing immense potential in biomedical applications, requires further advancements and interdisciplinary cooperation for its optimal utilization in revolutionizing medical science and benefiting patient healthcare. Full article