The 2023 MDPI Annual Report has
been released!
 
18 pages, 17598 KiB  
Article
Fisheye Object Detection with Visual Prompting-Aided Fine-Tuning
by Minwoo Jeon, Gyeong-Moon Park and Hyoseok Hwang
Remote Sens. 2024, 16(12), 2054; https://doi.org/10.3390/rs16122054 (registering DOI) - 7 Jun 2024
Abstract
Fisheye cameras play a crucial role in various fields by offering a wide field of view, enabling the capture of expansive areas within a single frame. Nonetheless, the radial distortion characteristics of fisheye lenses lead to notable shape deformation, particularly at the edges [...] Read more.
Fisheye cameras play a crucial role in various fields by offering a wide field of view, enabling the capture of expansive areas within a single frame. Nonetheless, the radial distortion characteristics of fisheye lenses lead to notable shape deformation, particularly at the edges of the image, posing a significant challenge for accurate object detection. In this paper, we introduce a novel method, ‘VP-aided fine-tuning’, which harnesses the strengths of the pretraining–fine-tuning paradigm augmented by visual prompting (VP) to bridge the domain gap between undistorted standard datasets and distorted fisheye image datasets. Our approach involves two key elements: the use of VPs to effectively adapt a pretrained model to the fisheye domain, and a detailed 24-point regression of objects to fit the unique distortions of fisheye images. This 24-point regression accurately defines the object boundaries and substantially reduces the impact of environmental noise. The proposed method was evaluated against existing object detection frameworks on fisheye images, demonstrating superior performance and robustness. Experimental results also showed performance improvements with the application of VP, regardless of the variety of fine-tuning method applied. Full article
(This article belongs to the Section Remote Sensing Image Processing)
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Article
Influence of the Manufacturing Method (3D Printing and Injection Molding) on Water Absorption and Mechanical and Thermal Properties of Polymer Composites Based on Poly(lactic acid)
by Paul Forbid Mukoroh, Fathi Gouda, Mikael Skrifvars and Sunil Kumar Ramamoorthy
Polymers 2024, 16(12), 1619; https://doi.org/10.3390/polym16121619 (registering DOI) - 7 Jun 2024
Abstract
The manufacturing method influences the properties of the produced components. This work investigates the influence of manufacturing methods, such as fused deposition modeling (3D printing) and injection molding, on the water absorption and mechanical and thermal properties of the specimens produced from neat [...] Read more.
The manufacturing method influences the properties of the produced components. This work investigates the influence of manufacturing methods, such as fused deposition modeling (3D printing) and injection molding, on the water absorption and mechanical and thermal properties of the specimens produced from neat bio-based poly(lactic acid) (PLA) polymer and poly(lactic acid)/wood composites. Acrylonitrile butadiene styrene (ABS) acts as the reference material due to its low water absorption and good functional properties. The printing layer thickness is one of the factors that affects the properties of a 3D-printed specimen. The investigation includes two different layer thicknesses (0.2 mm and 0.3 mm) while maintaining uniform overall thickness of the specimens across two manufacturing methods. 3D-printed specimens absorb significantly higher amounts of water than the injection-molded specimens, and the increase in the layer thickness of the 3D-printed specimens contributes to further increased water absorption. However, the swelling due to water absorption in 3D-printed specimens decreases upon increased layer thickness. The tensile, flexural, and impact properties of all of the specimens decrease after water absorption, while the properties improve upon decreasing the layer thickness. Higher porosity upon increasing the layer thickness is the predominant factor. The results from dynamic mechanical analysis and microscopy validate the outcomes. The results from this experimental study highlight the limitations of additive manufacturing. Full article
(This article belongs to the Special Issue Natural Fiber-Based Green Materials)
13 pages, 4017 KiB  
Article
Effects of Oil Concentration in Flood Cooling on Cutting Force, Tool Wear and Surface Roughness in GTD-111 Nickel-Based Superalloy Slot Milling
by Gábor Kónya and Zsolt F. Kovács
J. Manuf. Mater. Process. 2024, 8(3), 119; https://doi.org/10.3390/jmmp8030119 - 7 Jun 2024
Abstract
Cooling–lubricating processes have a big impact on cutting force, tool wear, and the quality of the machined surface, especially for hard-to-machine superalloys, so the choice of the right cooling–lubricating method is of great importance. Nickel-based superalloys are among the most difficult materials to [...] Read more.
Cooling–lubricating processes have a big impact on cutting force, tool wear, and the quality of the machined surface, especially for hard-to-machine superalloys, so the choice of the right cooling–lubricating method is of great importance. Nickel-based superalloys are among the most difficult materials to machine due to their high hot strength, work hardening, and extremely low thermal conductivity. Previous research has shown that flood cooling results in the least tool wear and cutting force among different cooling–lubricating methods. Thus, the effects of the flood oil concentration (3%; 6%; 9%; 12%; and 15%) on the above-mentioned factors were investigated during the slot milling of the GTD-111 nickel-based superalloy. The cutting force was measured during machining with a Kistler three-component dynamometer, and then after cutting the tool wear and the surface roughness on the bottom surface of the milled slots were measured with a confocal microscope and tactile roughness tester. The results show that at a 12% oil concentration, the tool load and tool wear are the lowest; even at an oil concentration of 15%, a slight increase is observed in both factors. Essentially, a higher oil concentration reduces friction between the tool and the workpiece contact surface, resulting in reduced tool wear and cutting force. Furthermore, due to less friction, the heat generation in the cutting zone is also reduced, resulting in a lower heat load on the tool, which increases tool life. It is interesting to note that the 6% oil concentration had the highest cutting force and tool wear, and strong vibration was heard during machining, which is also reflected in the force signal. The change in oil concentration did not effect the surface roughness. Full article
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24 pages, 376 KiB  
Article
Further Hermite–Hadamard-Type Inequalities for Fractional Integrals with Exponential Kernels
by Hong Li, Badreddine Meftah, Wedad Saleh, Hongyan Xu, Adem Kiliçman and Abdelghani Lakhdari
Fractal Fract. 2024, 8(6), 345; https://doi.org/10.3390/fractalfract8060345 - 7 Jun 2024
Abstract
This paper introduces new versions of Hermite–Hadamard, midpoint- and trapezoid-type inequalities involving fractional integral operators with exponential kernels. We explore these inequalities for differentiable convex functions and demonstrate their connections with classical integrals. This paper validates the derived inequalities through a numerical example [...] Read more.
This paper introduces new versions of Hermite–Hadamard, midpoint- and trapezoid-type inequalities involving fractional integral operators with exponential kernels. We explore these inequalities for differentiable convex functions and demonstrate their connections with classical integrals. This paper validates the derived inequalities through a numerical example with graphical representations and provides some practical applications, highlighting their relevance to special means. This study presents novel results, offering new insights into classical integrals as the fractional order β approaches 1, in addition to the fractional integrals we examined. Full article
(This article belongs to the Special Issue New Trends on Generalized Fractional Calculus)
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17 pages, 53744 KiB  
Article
Fractal Tent Map with Application to Surrogate Testing
by Ekaterina Kopets, Vyacheslav Rybin, Oleg Vasilchenko, Denis Butusov, Petr Fedoseev and Artur Karimov
Fractal Fract. 2024, 8(6), 344; https://doi.org/10.3390/fractalfract8060344 - 7 Jun 2024
Abstract
Discrete chaotic maps are a mathematical basis for many useful applications. One of the most common is chaos-based pseudorandom number generators (PRNGs), which should be computationally cheap and controllable and possess necessary statistical properties, such as mixing and diffusion. However, chaotic PRNGs have [...] Read more.
Discrete chaotic maps are a mathematical basis for many useful applications. One of the most common is chaos-based pseudorandom number generators (PRNGs), which should be computationally cheap and controllable and possess necessary statistical properties, such as mixing and diffusion. However, chaotic PRNGs have several known shortcomings, e.g., being prone to chaos degeneration, falling in short periods, and having a relatively narrow parameter range. Therefore, it is reasonable to design novel simple chaotic maps to overcome these drawbacks. In this study, we propose a novel fractal chaotic tent map, which is a generalization of the well-known tent map with a fractal function introduced into the right-hand side. We construct and investigate a PRNG based on the proposed map, showing its high level of randomness by applying the NIST statistical test suite. The application of the proposed PRNG to the task of generating surrogate data and a surrogate testing procedure is shown. The experimental results demonstrate that our approach possesses superior accuracy in surrogate testing across three distinct signal types—linear, chaotic, and biological signals—compared to the MATLAB built-in randn() function and PRNGs based on the logistic map and the conventional tent map. Along with surrogate testing, the proposed fractal tent map can be efficiently used in chaos-based communications and data encryption tasks. Full article
(This article belongs to the Topic Recent Trends in Nonlinear, Chaotic and Complex Systems)
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17 pages, 4092 KiB  
Article
Multi-UAV Formation Path Planning Based on Compensation Look-Ahead Algorithm
by Tianye Sun, Wei Sun, Changhao Sun and Ruofei He
Drones 2024, 8(6), 251; https://doi.org/10.3390/drones8060251 - 7 Jun 2024
Abstract
This study primarily studies the shortest-path planning problem for unmanned aerial vehicle (UAV) formations under uncertain target sequences. In order to enhance the efficiency of collaborative search in drone clusters, a compensation look-ahead algorithm based on optimizing the four-point heading angles is proposed. [...] Read more.
This study primarily studies the shortest-path planning problem for unmanned aerial vehicle (UAV) formations under uncertain target sequences. In order to enhance the efficiency of collaborative search in drone clusters, a compensation look-ahead algorithm based on optimizing the four-point heading angles is proposed. Building upon the receding-horizon algorithm, this method introduces the heading angles of adjacent points to approximately compensate and decouple the triangular equations of the optimal trajectory, and a general formula for calculating the heading angles is proposed. The simulation data indicate that the model using the compensatory look forward algorithm exhibits a maximum improvement of 12.9% compared to other algorithms. Furthermore, to solve the computational complexity and sample size requirements for optimal solutions in the Dubins multiple traveling salesman model, a path-planning model for multiple UAV formations is introduced based on the Euclidean traveling salesman problem (ETSP) pre-allocation. By pre-allocating sub-goals, the model reduces the computational scale of individual samples while maintaining a constant sample size. The simulation results show an 8.4% and 17.5% improvement in sparse regions for the proposed Euclidean Dubins traveling salesman problem (EDTSP) model for takeoff from different points. Full article
(This article belongs to the Special Issue Distributed Control, Optimization, and Game of UAV Swarm Systems)
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5 pages, 2050 KiB  
Interesting Images
Febrile Rash: An Early Diagnostic Clue to Infectious Illness in Travelers Returning from Thailand
by Hisham Ahmed Imad, Anastasia Putri, Ratchata Charoenwisedsil, Sakarn Charoensakulchai and Eric Caumes
Reports 2024, 7(2), 45; https://doi.org/10.3390/reports7020045 - 7 Jun 2024
Abstract
The eruption of a rash along with spiking fever in travelers returning from the tropics may be suspicious of arboviral diseases, and isolation prevent further transmission in non-endemic countries. The case presented here was seen at the Fever Clinic at the Hospital for [...] Read more.
The eruption of a rash along with spiking fever in travelers returning from the tropics may be suspicious of arboviral diseases, and isolation prevent further transmission in non-endemic countries. The case presented here was seen at the Fever Clinic at the Hospital for Tropical Diseases in Bangkok, Thailand. The presenting complaints were fever, headache, myalgia, and a distinctive erythematous blanching rash. Despite a negative dengue NS1 test on the initial day, anti-dengue IgM and IgG were detectable on day five of illness. Dengue, a leading cause of traveler’s fever with rash, is of particular concern, especially during outbreaks like the one in Thailand in 2023, when the number of cases exceeded one hundred thousand over a nine-month period. The influx of 28 million travelers in 2023, many with naive immunity to many arboviruses, raises fear of transmission to temperate regions, including to countries like France, where Aedes albopictus establishment can lead to autochthonous dengue cases and clusters. Enhanced surveillance is crucial, urging the consideration of dengue as a potential diagnosis in travelers with febrile rash, even prior to lab confirmation. Immediate isolation of patients is essential to prevent autochthonous transmission, reduce outbreak risks, and avert public health crises. Full article
(This article belongs to the Collection Health Threats of Climate Change)
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18 pages, 18979 KiB  
Article
Friction Stir Processed AA5754-Al2O3 Nanocomposite: A Study on Tribological Characteristics
by M. Nafea M. Rohim, Mahmoud E. Abdullah, Moustafa M. Mohammed, Andrzej Kubit and Hamed Aghajani Derazkola
J. Compos. Sci. 2024, 8(6), 216; https://doi.org/10.3390/jcs8060216 - 7 Jun 2024
Abstract
This study investigates the tribological properties of an AA 5754 aluminum alloy composite reinforced with the nanopowder of Al2O3, fabricated using the friction stir processing (FSP) technique with blind holes. The aim is to analyze the effects of varying [...] Read more.
This study investigates the tribological properties of an AA 5754 aluminum alloy composite reinforced with the nanopowder of Al2O3, fabricated using the friction stir processing (FSP) technique with blind holes. The aim is to analyze the effects of varying the tool rotational speed (rpm) and blind hole diameter on the wear and friction behavior of the produced composite. A pin-on disk test is conducted under dry conditions and room temperature to assess the tribological properties against steel. Scanning electron microscopy (SEM) coupled with energy dispersive X-ray spectroscopy (EDS) is employed to examine the worn and wear surfaces of the produced composites post test. The results indicate that increasing the applied load results in a decrease in the coefficient of friction (COF), with values ranging from 0.775 to 0.852 for 10 N and 0.607 to 0.652 for 20 N. Moreover, the wear rate diminishes with higher Al2O3 content and optimal FSP tool rotation (1280 rpm). Hardness analysis reveals variations between 33–42 HV and 35–39 HV, influenced by nanoparticle distribution. The composite demonstrates superior wear resistance compared to raw AA5754 aluminum due to its reinforced nature. However, high FSP tool rotation rates lead to abrasive wear and surface cracks. These findings offer insights into optimizing FSP parameters to enhance the tribological performance of nano-reinforced aluminum alloys. Full article
(This article belongs to the Special Issue Metal Composites, Volume II)
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28 pages, 5234 KiB  
Review
Mechanical Performance of Recycled 3D Printed Sustainable Polymer-Based Composites: A Literature Review
by Ioannis Filippos Kyriakidis, Nikolaos Kladovasilakis, Eleftheria Maria Pechlivani and Konstantinos Tsongas
J. Compos. Sci. 2024, 8(6), 215; https://doi.org/10.3390/jcs8060215 - 7 Jun 2024
Abstract
The development of efficient waste valorization strategies has emerged as an important field in the overall efforts for alignment with the environmental goals that have been set by the European Union (EU) Green Deal regarding the development of sustainable circular economy models. Additive [...] Read more.
The development of efficient waste valorization strategies has emerged as an important field in the overall efforts for alignment with the environmental goals that have been set by the European Union (EU) Green Deal regarding the development of sustainable circular economy models. Additive manufacturing has emerged as a sustainable method for secondary life product development with the main advantages of it being a form of net-zero waste production and having the ability to successfully transport complex design to actual products finding applications in the industry for rapid prototyping or for tailored products. The insertion of eco-friendly sustainable materials in these processes can lead to significant reduction in material footprints and lower energy demands for the manufacturing process, helping achieve Sustainable Development Goal 12 (SDG12) set by the EU for responsible production and consumption. The aim of this comprehensive review is to state the existing progress regarding the incorporation of sustainable polymeric composite materials in additive manufacturing (AM) processes and identify possible gaps for further research. In this context, a comprehensive presentation of the reacquired materials coming from urban and industrial waste valorization processes and that are used to produce sustainable composites is made. Then, an assessment of the printability and the mechanical response of the constructed composites is made, by taking into consideration some key thermal, rheological and mechanical properties (e.g., viscosity, melting and degradation temperature, tensile and impact strength). Finally, existing life cycle analysis results are presented regarding overall energy demands and environmental footprint during the waste-to-feedstock and the manufacturing processes. A lack of scientific research was observed, regarding the manifestation of novel evaluation techniques such as dynamic mechanical analysis and impact testing. Assessing the dynamic response is vital for evaluating whether these types of composites are adequate for upscaling and use in real life applications. Full article
(This article belongs to the Special Issue Recycled Polymer Composites: Futuristic Sustainable Material)
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18 pages, 2970 KiB  
Article
A Study of the Moisture Absorption Characteristics of Vinyl Ester Polymer and Unidirectional Glass Fibre Vinyl Ester Laminates
by James Thomason and Georgios Xypolias
J. Compos. Sci. 2024, 8(6), 214; https://doi.org/10.3390/jcs8060214 - 7 Jun 2024
Abstract
Vinyl esters are increasingly being used as the matrix polymer in fibre-reinforced composites for demanding large applications which experience long-term exposure to moist and wet conditions. This paper presents the results of a study of ageing due to moisture absorption in vinyl ester [...] Read more.
Vinyl esters are increasingly being used as the matrix polymer in fibre-reinforced composites for demanding large applications which experience long-term exposure to moist and wet conditions. This paper presents the results of a study of ageing due to moisture absorption in vinyl ester polymer and glass fibre–vinyl ester laminates. The moisture uptake kinetics of the two neat VE polymers, cured at different conditions, and their glass-reinforced composites has been characterised by gravimetric methods. These studies have been carried out using submersion in water at 23 °C and 50 °C and exposure to high relative humidity moisture conditions at room temperature. A dynamic mechanical analysis characterisation of the glass transition temperatures of both the aged matrix and the composite is also presented. Full article
(This article belongs to the Special Issue Polymer Composites and Fibers, Volume II)
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13 pages, 2663 KiB  
Article
Frequency-Specific Responses: The Impact of an Acoustic Stimulus on Behavioral and Physiological Indices in Large Yellow Croaker
by Xiaojie Cui, Pengxiang Xu, Tao Tian, Mingyuan Song, Xuyang Qin, Dehua Gong, Yan Wang, Xuguang Zhang, Binbin Xing, Mingzhi Li and Leiming Yin
Fishes 2024, 9(6), 217; https://doi.org/10.3390/fishes9060217 - 7 Jun 2024
Abstract
This study assessed the impact of an acoustic stimulus on the behavioral responses and physiological states of the large yellow croaker (Larimichthys crocea). The test fish, with an average body weight of approximately 352.81 ± 70.99 g, were exposed to one [...] Read more.
This study assessed the impact of an acoustic stimulus on the behavioral responses and physiological states of the large yellow croaker (Larimichthys crocea). The test fish, with an average body weight of approximately 352.81 ± 70.99 g, were exposed to one hour of acoustic stimulation at seven different frequencies: 100 Hz, 125 Hz, 160 Hz, 200 Hz, 500 Hz, 630 Hz, and 800 Hz. The aim was to delineate the specific effects of acoustic stimulation on the behavior and physiological indices. The results show that acoustic stimulation significantly altered the behavioral patterns of the large yellow croaker, predominantly manifested as avoidance behavior towards the sound source. At a stimulus frequency of 630 Hz, the test fish exhibited continuous irregular motion and erratic swimming. Physiologically, one hour of exposure to acoustic stimulation notably affected the endocrine system. The levels of Epinephrine and thyroxine were significantly elevated at 200 Hz, while the cortisol levels did not show significant differences. Additionally, the lactic acid content significantly increased at 800 Hz, and the blood glucose content peaked at 630 Hz. This study discovered that sound frequencies of 200 Hz, 630 Hz, and 800 Hz led to a significant increase in the levels of Epinephrine, glucose, thyroid hormones, and lactate in large yellow croaker, consequently affecting their behavior. The changes in these physiological indicators reflect the stress response of the large yellow croaker in specific sonic environments, providing crucial insights into the physiological and behavioral responses of fish to acoustic stimuli. Full article
(This article belongs to the Special Issue Assessment and Management of Fishery Resources)
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15 pages, 236 KiB  
Article
Exploring Community Garden Coordinators’ Perceptions of Climate-Smart Adaptations to Support Local Food Systems
by Olivia M. Erskine, Alexa J. Lamm, Catherine E. Sanders and Kevan W. Lamm
Horticulturae 2024, 10(6), 601; https://doi.org/10.3390/horticulturae10060601 - 7 Jun 2024
Abstract
Extreme weather events, increased intensity of droughts and floods, and changes to growing seasons are results of climate change that impact horticulture, agriculture, and food systems. In the United States, Georgia, North Carolina, and South Carolina experience similar impacts caused by climate change [...] Read more.
Extreme weather events, increased intensity of droughts and floods, and changes to growing seasons are results of climate change that impact horticulture, agriculture, and food systems. In the United States, Georgia, North Carolina, and South Carolina experience similar impacts caused by climate change such as rising sea levels and extreme heat. In these states, community gardens can be a source of local, fresh foods, especially in areas experiencing food insecurity. The goals of this study were to identify garden coordinators’ perceptions of the need for climate change adaptation, perceptions regarding the five perceived attributes of climate change adaptation, and where community garden coordinators stand in the innovation–decision process when it comes to climate change adaptation. The findings show that participants valued relative advantage and low levels of complexity when adopting and implementing climate-smart practices into their gardens. This study found that the community gardens were all implementing some form of climate-smart adaptations even if implementation was not for climate-related reasons. All participants noted that the largest barrier to adopting new practices was a lack of extra money. The findings from this study should be used to inform environmental education and communication strategies that encourage adoption of climate-smart practices. Full article
(This article belongs to the Special Issue Rethinking Horticulture to Meet Sustainable Development Goals)
12 pages, 4258 KiB  
Article
Comparative Metabolomic Fingerprinting Analysis of Tomato Fruits from Physalis Species in Mexico’s Balsas Basin
by Sergio Arias-Martínez, Guadalupe Oyoque-Salcedo, Oscar Giovanni Gutiérrez-Cárdenas, Ernesto Oregel-Zamudio and Jesús Rubén Torres-García
Horticulturae 2024, 10(6), 600; https://doi.org/10.3390/horticulturae10060600 - 7 Jun 2024
Abstract
This study investigated the chemical and sensory distinctions in tomato fruits from three Physalis species (P. ixocarpa, P. angulata, and P. philadelphica) found in Michoacán, Mexico, using metabolomic fingerprinting through GC-MS analysis. The objective was to identify organoleptic differences that could [...] Read more.
This study investigated the chemical and sensory distinctions in tomato fruits from three Physalis species (P. ixocarpa, P. angulata, and P. philadelphica) found in Michoacán, Mexico, using metabolomic fingerprinting through GC-MS analysis. The objective was to identify organoleptic differences that could influence consumer preferences, highlighting the significance of these species’ unique traits. These species represented a valuable genetic reservoir for potential hybridization or selection aimed at enhancing commercial varieties by focusing on organoleptic properties rather than traditional selection criteria like fruit size or yield. This research emphasizes the importance of preserving Mexican biodiversity and providing insights into domestication processes that prioritize flavor and sensory qualities. By analyzing metabolite profiles and their correlation with taste preferences, this study contributes to understanding how these differences could be leveraged in breeding programs to develop new tomato varieties with preferred flavors. It was suggested that variations in taste among the species are mainly due to differences in metabolite expression. This knowledge underscores the importance of organoleptic properties in the selection and domestication of edible fruits, offering a pathway toward the conservation and enhancement of tomato varieties through the exploitation of genetic diversity for organoleptic improvement. Full article
(This article belongs to the Special Issue Fruits Quality and Sensory Analysis)
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16 pages, 5747 KiB  
Article
Overexpression of the CpCOR413PM1 Gene from Wintersweet (Chimonanthus praecox) Enhances Cold and Drought Tolerance in Arabidopsis
by Yeyuan Deng, Yi Lin, Guo Wei, Xiaoqian Hu, Yanghui Zheng and Jing Ma
Horticulturae 2024, 10(6), 599; https://doi.org/10.3390/horticulturae10060599 - 7 Jun 2024
Abstract
Wintersweet (Chimonanthus praecox (L.) Link) is a commercial significance woody plant. As a rare winter-blooming plant, C. praecox is widely distributed and highly adaptable to various climates, especially low temperatures. In this study, we identified the COR413 plasma membrane gene CpCOR413PM1 in [...] Read more.
Wintersweet (Chimonanthus praecox (L.) Link) is a commercial significance woody plant. As a rare winter-blooming plant, C. praecox is widely distributed and highly adaptable to various climates, especially low temperatures. In this study, we identified the COR413 plasma membrane gene CpCOR413PM1 in wintersweet. CpCOR413PM1 was expressed in all tissues of the plant, with the highest expression observed in the root and floral parts. Cultivation under 4 °C and with treatment of ABA led to the up-regulated expression of CpCOR413PM1. The expression of recombinant CpCOR413PM1 protein in Escherichia coli resulted in the tissues’ increased resilience to cold and drought stress. In vitro enzyme activity assays confirmed the protective impact of the CpCOR413PM1 protein on superoxide dismutase under low-temperature stress. Furthermore, the overexpression of CpCOR413PM1 in Arabidopsis thaliana resulted in increased cold and drought tolerance and ABA inhibited transgenic Arabidopsis seed germination. The CpCOR413PM1 gene promoter can influence expression of the GUS reporter gene under conditions of 4 °C, 42 °C and abscisic acid. Overall, our study demonstrates that CpCOR413PM1 plays a significate role in cold and drought stress. Our findings strengthen the knowledge of the molecular mechanisms underlying wintersweet’s tolerance to stress and lay the groundwork for the future investigation of the functions of the COR gene family. Full article
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15 pages, 1534 KiB  
Article
Naturally Colored Ice Creams Enriched with C-Phycocyanin and Spirulina Residual Biomass: Development of a Fermented, Antioxidant, Tasty and Stable Food Product
by Monize Bürck, Camilly Fratelli, Marcelo Assis and Anna Rafaela Cavalcante Braga
Fermentation 2024, 10(6), 304; https://doi.org/10.3390/fermentation10060304 - 7 Jun 2024
Abstract
Naturally colored fermented foods currently represent the trend toward a global demand for healthier products. This work produced naturally blue and green ice creams using C-phycocyanin (C-PC) and spirulina residual biomass (RB). The ice creams were assessed based on microbiological analysis, color stability [...] Read more.
Naturally colored fermented foods currently represent the trend toward a global demand for healthier products. This work produced naturally blue and green ice creams using C-phycocyanin (C-PC) and spirulina residual biomass (RB). The ice creams were assessed based on microbiological analysis, color stability over 6 months, antioxidant activity before and after in vitro digestion, and sensory evaluation. Considering the microorganisms that must be analyzed in accordance with Brazilian legislation, no growth was detected during the storage period. L*, a*, and b* were maintained according to the expected colors. The blue color was intoned over the shelf life (SC-PC *b −9.46 to −19.44 and MC-PC *b from −9.87 to −18.04). The antioxidant activity of the fermented ice creams SC-PC and SRB increased from 15.4 to 41.3 and from 15.3 to 38.0 µM TE/g, respectively, after bioaccessibility analysis. The C-PC ice cream’s appearance received the highest rating, with 70.26% of volunteers expressing a strong preference, highlighting its attractiveness. However, there were no significant differences compared to control samples in the global acceptance. The RB ice cream presented lower results for flavor but moderate acceptance. Thus, these fermented ice creams presented color stability over 6 months, and their antioxidant activity increased after in vitro digestion, highlighting their biological potential. Full article
(This article belongs to the Special Issue Production and Purification of Microbial Dyes and Pigments)
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16 pages, 11322 KiB  
Article
In Situ Processing to Achieve High-Performance Epoxy Nanocomposites with Low Graphene Oxide Loading
by Miraidin Mirzapour, Mathieu Robert and Brahim Benmokrane
C 2024, 10(2), 52; https://doi.org/10.3390/c10020052 - 7 Jun 2024
Abstract
Modifying the polymer matrix by nanoparticles can be a promising approach to improve the performance of fiber-reinforced polymer (FRP) composites. Organic solvents are usually used for dispersing graphene oxide (GO) well in the polymer matrix. In this study, a green, facile, and efficient [...] Read more.
Modifying the polymer matrix by nanoparticles can be a promising approach to improve the performance of fiber-reinforced polymer (FRP) composites. Organic solvents are usually used for dispersing graphene oxide (GO) well in the polymer matrix. In this study, a green, facile, and efficient approach was developed to prepare epoxy/GO nanocomposites. In situ polymerization is used for synthesizing nanocomposites, eliminating the need for organic solvents and surfactants. By loading just 0.6 wt% of GO into the epoxy resin, Young’s modulus, tensile strength, and toughness improved by 38%, 46%, and 143%, respectively. Fractography analysis indicates smooth fracture surfaces of pure resin that changed to highly toughened fracture surfaces in this nanocomposite. Plastic deformation, crack pinning, and deflection contributed to improving the toughness of the nanocomposites. FTIR investigations show that amide bonding was created by the reaction of the carboxylic acid groups in GO with some amine groups in the curing agent during the dispersion processes. Full article
(This article belongs to the Special Issue High-Performance Carbon Materials and Their Composites)
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16 pages, 1477 KiB  
Article
Exploring the Resilience of Islamic Stock in Indonesia and Asian Markets
by Nofrianto Nofrianto, Deni Pandu Nugraha, Amanj Mohamed Ahmed, Zaenal Muttaqin, Maria Fekete-Farkas and István Hágen
J. Risk Financial Manag. 2024, 17(6), 239; https://doi.org/10.3390/jrfm17060239 - 7 Jun 2024
Abstract
This study aims to investigate the relationship between returns and risk of Islamic stock under stable economic conditions, crises, and pandemics within the scope of Indonesian and Asian Islamic capital markets. How do economic conditions affect the risks and returns of investors in [...] Read more.
This study aims to investigate the relationship between returns and risk of Islamic stock under stable economic conditions, crises, and pandemics within the scope of Indonesian and Asian Islamic capital markets. How do economic conditions affect the risks and returns of investors in the Indonesian and Asian Islamic capital markets? Verification of the veracity of the Islamic capital market serves as a more resilient option for alternative investments. This study uses Granger causality to determine exogenous and endogenous variables when building the model. The model that is formed is then analyzed using regression with dummy variables of stable economic conditions, crises, and pandemics. The first research findings on differences in crisis, stable and pandemic times in the Asian stock market show that there is no significant difference in effect between stable times and during a crisis, but there are differences in the effect during stable and pandemic times. The second research finding states that the return on Asian market Shariah stocks has no influence on increasing or reducing the value of risk or value at risk. The third finding explains that Islamic stocks in Indonesia have a greater risk value during pandemics and crises than in stable times, but the effect of pandemic and crisis conditions is not as great as Islamic stocks in Asia as a whole. In order to stabilize markets and reduce risks, regulatory bodies and governments frequently employ a variety of actions during times of crisis. When applied to trading volume, risk, and return patterns, these findings can help determine the appropriate policy. Full article
(This article belongs to the Section Financial Markets)
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14 pages, 253 KiB  
Article
CEO Characteristics and Risk-Taking under Economic Policy Uncertainty
by Ivan Stetsyuk, Ayca Altintig, Kerim Peren Arin and Moo Sung Kim
J. Risk Financial Manag. 2024, 17(6), 238; https://doi.org/10.3390/jrfm17060238 - 7 Jun 2024
Abstract
This paper investigates the effects of such CEO characteristics as gender, age, and education on the CEOs’ risk-taking behavior during periods of economic policy uncertainty. The paper utilizes Execucomp, BoardEx, and Compustat data from 2005 to 2017 in order to give a novel [...] Read more.
This paper investigates the effects of such CEO characteristics as gender, age, and education on the CEOs’ risk-taking behavior during periods of economic policy uncertainty. The paper utilizes Execucomp, BoardEx, and Compustat data from 2005 to 2017 in order to give a novel perspective on how CEO characteristics may provide differing risk-taking positions when faced with varying levels of uncertainty. The results offer robust evidence that older CEOs generally take less risk—regardless of the level of economic policy uncertainty. However, more educated CEOs take less risk only during economically uncertain times. The results also indicate that while female CEOs tend to be younger and have lower levels of education, gender does not provide a significant difference in risk-taking behavior during periods of economic policy uncertainty. Furthermore, we do not find any significant effect of insider status or corporate governance variables on CEO risk-taking under economic policy uncertainty once gender, age, and education are controlled for. Full article
(This article belongs to the Special Issue Economic Policy Uncertainty)
10 pages, 7985 KiB  
Article
Establishment and Characterization of a New Cell Line from Enzootic Nasal Adenocarcinoma in Goats: ENA-1
by Lingxu Li, Weiye Tan, Zhen Wang, Wenqing Guo, Deji Yang and Dawei Yao
Vet. Sci. 2024, 11(6), 260; https://doi.org/10.3390/vetsci11060260 - 7 Jun 2024
Abstract
Enzootic nasal adenocarcinoma (ENA) is a contagious tumor disease of goats and sheep, which is caused by enzootic nasal tumor virus (ENTV). To better understand the pathogenesis of ENA, this study aimed to establish a goat ENA cell line (ENA-1). The cells have [...] Read more.
Enzootic nasal adenocarcinoma (ENA) is a contagious tumor disease of goats and sheep, which is caused by enzootic nasal tumor virus (ENTV). To better understand the pathogenesis of ENA, this study aimed to establish a goat ENA cell line (ENA-1). The cells have been characterized with regard to morphology, growth rate, ultrastructural features, chromosome number, expression of CK7 and CK18, tumorigenicity, species, and mycoplasma contamination. ENA-1 had an epithelioid cell morphology with an unstable chromosome number under a light microscope. Under an electron microscope, the cell nuclear heterogeneity was not obvious, and there were more intermediate filaments and a small number of immature retrovirus-like particles in the cytoplasm. ENA-1 had strong proliferative potential, and the cell multiplication time was about 36 h, which could make BALB/c nude mice develop tumors. CK7 and CK18 were expressed in the cytoplasm of primary goat tumors, in transplanted tumors from nude mice, and un ENA-1 cells with the same intensity. PCR revealed that ENA-1 continuously carried ENTV-2 up to the 17th generation with no germline contamination or mycoplasma contamination. In conclusion, using a serum-containing culture system, ENA-1 cells were successfully isolated, cultured, and purified from goat tumor tissues. The isolated ENA-1 cells retained robust proliferation potential and maintained their phenotype, indicating the potential application of the ENA-1 cell line as an in vitro model of ENA. Full article
(This article belongs to the Special Issue Comparative Oncology and Veterinary Cancer Surveillance)
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14 pages, 5268 KiB  
Article
West Nile Virus Seroprevalence in Wild Birds and Equines in Madrid Province, Spain
by Richard A. J. Williams, Hillary A. Criollo Valencia, Irene López Márquez, Fernando González González, Francisco Llorente, Miguel Ángel Jiménez-Clavero, Núria Busquets, Marta Mateo Barrientos, Gustavo Ortiz-Díez and Tania Ayllón Santiago
Vet. Sci. 2024, 11(6), 259; https://doi.org/10.3390/vetsci11060259 - 7 Jun 2024
Abstract
West Nile virus (WNV) is a re-emerging flavivirus, primarily circulating among avian hosts and mosquito vectors, causing periodic outbreaks in humans and horses, often leading to neuroinvasive disease and mortality. Spain has reported several outbreaks, most notably in 2020 with seventy-seven human cases [...] Read more.
West Nile virus (WNV) is a re-emerging flavivirus, primarily circulating among avian hosts and mosquito vectors, causing periodic outbreaks in humans and horses, often leading to neuroinvasive disease and mortality. Spain has reported several outbreaks, most notably in 2020 with seventy-seven human cases and eight fatalities. WNV has been serologically detected in horses in the Community of Madrid, but to our knowledge, it has never been reported from wild birds in this region. To estimate the seroprevalence of WNV in wild birds and horses in the Community of Madrid, 159 wild birds at a wildlife rescue center and 25 privately owned equines were sampled. Serum from thirteen birds (8.2%) and one equine (4.0%) tested positive with a WNV competitive enzyme-linked immunosorbent assay (cELISA) designed for WNV antibody detection but sensitive to cross-reacting antibodies to other flaviviruses. Virus-neutralization test (VNT) confirmed WNV antibodies in four bird samples (2.5%), and antibodies to undetermined flavivirus in four additional samples. One equine sample (4.0%) tested positive for WNV by VNT, although this horse previously resided in a WN-endemic area. ELISA-positive birds included both migratory and resident species, juveniles and adults. Two seropositive juvenile birds suggest local flavivirus transmission within the Community of Madrid, while WNV seropositive adult birds may have been infected outside Madrid. The potential circulation of flaviviruses, including WNV, in birds in the Madrid Community raises concerns, although further surveillance of mosquitoes, wild birds, and horses in Madrid is necessary to establish the extent of transmission and the principal species involved. Full article
(This article belongs to the Special Issue Wild Birds as Sentinels of the Health Status of the Environment)
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23 pages, 4341 KiB  
Article
Unleashing the Power of Closed-Loop Supply Chains: A Stackelberg Game Analysis of Rare Earth Resources Recycling
by Chenghao Lai, Xiuli Wang, Hengkai Li and Yanbing Zhou
Sustainability 2024, 16(12), 4899; https://doi.org/10.3390/su16124899 (registering DOI) - 7 Jun 2024
Abstract
Due to the rapid development of the clean energy sector against the global backdrop of carbon emission reduction, the availability of rare earths is becoming scarce. The strategic recycling of rare earth resources serves the dual purpose of alleviating supply shortages and aligning [...] Read more.
Due to the rapid development of the clean energy sector against the global backdrop of carbon emission reduction, the availability of rare earths is becoming scarce. The strategic recycling of rare earth resources serves the dual purpose of alleviating supply shortages and aligning with the global quest for carbon reduction and environmental preservation. This study establishes a second-level, closed-loop supply chain that includes a rare earth group (REG) that synergizes rare earth mining and smelting companies with rare earth material manufacturing companies, as well as a specialized rare earth recycler. The study considers three different recycling scenarios: the recycler only recycles oil sludge scrap (S1), the recycler only recycles end-of-life products (S2), and the recycler both recycles oil sludge scrap and end-of-life products (S3). In addition, the study examines the impact of government subsidies, carbon trading mechanisms, and corporate research and development (R&D) initiatives on closed-loop supply chains. The findings are summarized below. (1) Rare earth recycling does not impact the rare earth market downstream. (2) The profits of both REG and the recycler have a positive correlation with the quantity of recycling. However, recycling sludge waste can generate higher revenues for the recycler compared to recycling end-of-life products. (3) Government subsidies exert a positive effect on the recycler’s profits and the production of secondary rare earths. However, the REG redirects a segment of the recycler’s profits in its direction. Elevated carbon trading prices encroach upon the REG’s profitability, prompting increased procurement of secondary supply rare earths. (4) The benefits of increasing rare earth recycling rates outweigh government subsidies and carbon trading valuations in the supply chain. Therefore, enterprise-focused research and development initiatives play a critical role in improving the efficiency of the closed-loop rare earth supply chain. Full article
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10 pages, 1582 KiB  
Article
Survival Rates of Amalgam and Composite Resin Restorations from Big Data Real-Life Databases in the Era of Restricted Dental Mercury Use
by Guy Tobias, Tali Chackartchi, Jonathan Mann, Doron Haim and Mordechai Findler
Bioengineering 2024, 11(6), 579; https://doi.org/10.3390/bioengineering11060579 - 7 Jun 2024
Abstract
Tooth decay, also known as caries, is a significant medical problem that harms teeth. Treatment is based on the removal of the carious material and then filling the cavity left in the tooth, most commonly with amalgam or composite resin. The consequences of [...] Read more.
Tooth decay, also known as caries, is a significant medical problem that harms teeth. Treatment is based on the removal of the carious material and then filling the cavity left in the tooth, most commonly with amalgam or composite resin. The consequences of filling failure include repeating the filling or performing another treatment such as a root canal or extraction. Dental amalgam contains mercury, and there is a global effort to reduce its use. However, no consensus has been reached regarding whether amalgam or composite resin materials are more durable, and which is the best restorative material, when using randomized clinical trials. To determine which material is superior, we performed a retrospective cohort study using a large database where the members of 58 dental clinics with 440 dental units were treated. The number of failures of the amalgam compared to composite resin restorations between 2014 and 2021 were compared. Our data included information from over 650,000 patients. Between 2014–2021, 260,905 patients were treated. In total, 19,692 out of the first 113,281 amalgam restorations failed (17.49%), whereas significantly fewer composite restorations failed (11.98%) with 65,943 out of 555,671. This study indicates that composite is superior to amalgam and therefore it is reasonable to cease using mercury-containing amalgam. Full article
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12 pages, 1901 KiB  
Article
Visualized Lead Selection for Arrhythmia Classification Based on a Lead Activation Heatmap Using Multi-Lead ECGs
by Heng Wang, Tengqun Shen, Shoufen Jiang, Jilin Wang, Yijun Ma and Yatao Zhang
Bioengineering 2024, 11(6), 578; https://doi.org/10.3390/bioengineering11060578 - 7 Jun 2024
Abstract
Visualizing the decision-making process is a key aspect of research regarding explainable arrhythmia recognition. This study proposed a visualized lead selection method to classify arrhythmia for multi-lead ECG signals. The proposed method has several advantages, as it uses a visualized approach to select [...] Read more.
Visualizing the decision-making process is a key aspect of research regarding explainable arrhythmia recognition. This study proposed a visualized lead selection method to classify arrhythmia for multi-lead ECG signals. The proposed method has several advantages, as it uses a visualized approach to select effective leads, avoiding redundant leads and invalid information. It also captures the temporal dependencies of ECG signals and the complementary information between leads. The method deployed a lead activation heatmap (LA heatmap) based on a lead-wise network to select the proper 5 leads from 12-lead ECG heartbeats extracted from the public 2018 Chinese Physiological Signal Challenge database (CPSC 2018 DB), which were then fed into a ResBiTime network combining bidirectional long short-term memory (Bi-LSTM) networks and residual connections for a classification task of nine heartbeat categories (i.e., N, AF, I-AVB, RBBB, PAC, PVC, STD, LBBB, and STE). The results indicate an average precision of 93.25%, an average recall of 93.03%, an average F1-score of 0.9313, and that the proposed method can effectively extract additional information from ECG heartbeat data. Full article
(This article belongs to the Special Issue 10th Anniversary of Bioengineering: Biosignal Processing)
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