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Identification of differential gene expression for Kr1 gene in bread wheat using annealing control primer system

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Abstract

Intergeneric hybridization is an important strategy to introgress alien genes into common wheat for its improvement. But presence of cross ability barrier mechanism regulated by Kr1 gene played a major destructive role for hybridization than other reported genes. In order to know the underlying molecular mechanism and to dissect out this barrier, a new annealing system, ACP (anneling control primer) system was used in chromosome 5B (containing Kr1 gene) specific Recombinant Inbred Line (RIL) population. Two differentially expressed fragments for Kr1 gene was identified, cloned and sequenced. Further the expression was confirmed by northern blotting analysis. Sequence analysis of the resulted clones revealed classes of putative genes, including stress responsive and signal transduction.

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Abbreviations

RIL:

Recombinant inbred lines

ACP:

Annealing control primer

PCR:

Polymerase chain reaction

DEF:

Differentially expressed fragment

PTI:

Pto interacting protein

CDPK:

Calcium dependent protein kinase

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Authors and Affiliations

  1. Faculty of Horticulture, Chiba University, 648 Matsudo, 271-8510, Japan

    Alagu Manickavelu, Takato Koba, Kohei Mishina & Hidenori Sassa

  2. Laboratory of Plant Genome Science, Kihara Institute for Biological Research, Yokohama City University, Maioka-cho 641-12, Totsuka-ku, Yokohama, 244-0813, Japan

    Alagu Manickavelu

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  1. Alagu Manickavelu
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  2. Takato Koba
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  3. Kohei Mishina
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  4. Hidenori Sassa
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Correspondence to Alagu Manickavelu.

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Manickavelu, A., Koba, T., Mishina, K. et al. Identification of differential gene expression for Kr1 gene in bread wheat using annealing control primer system. Mol Biol Rep 36, 2111–2118 (2009). https://doi.org/10.1007/s11033-008-9423-0

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  • DOI: https://doi.org/10.1007/s11033-008-9423-0

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