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Association of LRP5 haplotypes with osteoporosis in Mexican women

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Abstract

Osteoporosis is a common health problem in Mexico, so it is essential to investigate the status of different gene polymorphisms that could serve as genetic susceptibility markers in the Mexican population. Genes with a role in bone metabolism are excellent candidates for association studies. In this study were determined the allelic and genotypic frequencies of four polymorphic markers (C/T rs3736228, G/A rs4988321, T/C rs627174 and T/C rs901824) in the low-density lipoprotein receptor-related protein 5 gene (LRP5) and their association with osteoporosis in 100 pos-menopausal osteoporotic Mexican women and their controls, using real time-PCR and TaqMan probes. Only the G/A polymorphism (rs4988321, Val667Met) showed significant differences (p = 0.039) when genotype frequencies were compared. However, when the haplotypes of these four polymorphisms were analyzed, interesting associations became evident. The CGTT haplotype showed significant association with low risk of osteoporosis (OR 0.629; p = 0.007; [95 % CI, 0.448–0.884]), whereas the TACT haplotype was significantly associated with a higher risk of osteoporosis (OR 7.965; p = 0.006; [95 % CI, 1.557–54.775]). Our results supported the association of LRP5 with osteoporosis and showed the potential value of LRP5 haplotypes to identify risk of osteoporosis in Mexican population.

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Acknowledgments

This work was supported by Consejo Nacional de Ciencia y Tecnología SALUD-2007-C01-69706.

Author information

Authors and Affiliations

  1. Dirección de Investigación, Servicio de Genética, Instituto Nacional de Rehabilitación, Calzada México-Xochimilco No. 289 Arenal de Guadalupe, 14389, México, D.F., México

    Edith Falcón-Ramírez, Leonora Casas-Avila & Margarita Valdés-Flores

  2. Facultad de Enfermería y Centro de Investigación y Desarrollo de Ciencias de la Salud UANL, Monterrey, Nuevo León, México

    Ricardo M. Cerda-Flores

  3. Departmento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, UNAM, México, D.F., México

    Clementina Castro-Hernández & Julieta Rubio-Lightbourn

  4. Instituto Nacional de Medicina Genómica, México, D.F., México

    Rafael Velázquez-Cruz

  5. Clínica de Osteoporosis, Instituto Nacional de Rehabilitación, México, D.F., México

    Pilar Diez-G

  6. Departamento de Sistemas Biológicos, UAM-X, México, D.F., México

    Rosenda Peñaloza-Espinosa

Authors
  1. Edith Falcón-Ramírez
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  2. Leonora Casas-Avila
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  3. Ricardo M. Cerda-Flores
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  4. Clementina Castro-Hernández
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  5. Julieta Rubio-Lightbourn
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  6. Rafael Velázquez-Cruz
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  7. Pilar Diez-G
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  8. Rosenda Peñaloza-Espinosa
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  9. Margarita Valdés-Flores
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Corresponding author

Correspondence to Margarita Valdés-Flores.

Additional information

Leonora Casas-Avila and Edith Falcón-Ramírez contributed equally to this study.

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Falcón-Ramírez, E., Casas-Avila, L., Cerda-Flores, R.M. et al. Association of LRP5 haplotypes with osteoporosis in Mexican women. Mol Biol Rep 40, 2705–2710 (2013). https://doi.org/10.1007/s11033-012-2357-6

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  • DOI: https://doi.org/10.1007/s11033-012-2357-6

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