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Vascular biomechanical properties in mice with smooth muscle specific deletion of Ndst1

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Abstract

Heparan sulfate proteoglycans act as co-receptors for many chemokines and growth factors. The sulfation pattern of the heparan sulfate chains is a critical regulatory step affecting the binding of chemokines and growth factors. N-deacetylase-N-sulfotransferase1 (Ndst1) is one of the first enzymes to catalyze sulfation. Previously published work has shown that HSPGs alter tangent moduli and stiffness of tissues and cells. We hypothesized that loss of Ndst1 in smooth muscle would lead to significant changes in heparan sulfate modification and the elastic properties of arteries. In line with this hypothesis, the axial tangent modulus was significantly decreased in aorta from mice lacking Ndst1 in smooth muscle (SM22αcre+Ndst1−/−, p < 0.05, n = 5). The decrease in axial tangent modulus was associated with a significant switch in myosin and actin types and isoforms expressed in aorta and isolated aortic vascular smooth muscle cells. In contrast, no changes were found in the compliance of smaller thoracodorsal arteries of SM22αcre+Ndst1−/− mice. In summary, the major findings of this study were that targeted ablation of Ndst1 in smooth muscle cells results in altered biomechanical properties of aorta and differential expression of myosin and actin types and isoforms.

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Acknowledgments

This study was funded by a R01 to JLH (National Institute of Health-R01HL081715). Special thanks to the staff of the Histology Core facility, Lillehei Heart Institute, and Cynthia Dekay, Graphic Designer, Lillehei Heart Institute, for their help in the preparation of this manuscript.

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Authors and Affiliations

  1. Lillehei Heart Institute, Department of Medicine, University of Minnesota, 4-106 NHH, 312 Church St. SE, Minneapolis, MN, 55455, USA

    Neeta Adhikari, Marjorie Carlson, Kim Ramil C. Montaniel, Rod Staggs, Snider Desir & Jennifer L. Hall

  2. Robert M Berne Cardiovascular Research Center, School of Medicine, University of Virginia, Charlottesville, VA, 22908, USA

    Marie Billaud & Brant E. Isakson

  3. Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN, 55455, USA

    Spencer P. Lake & Victor H. Barocas

  4. Biomedical Genomics Center, University of Minnesota, Minneapolis, MN, 55455, USA

    Dinesha Walek

  5. Division of Cardiology, Department of Medicine, University of Minnesota, Minneapolis, MN, 55455, USA

    Jennifer L. Hall

  6. Department of Biostatistics, University of Minnesota, Minneapolis, MN, 55455, USA

    Weihua Guan

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  1. Neeta Adhikari
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Correspondence to Jennifer L. Hall.

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Adhikari, N., Billaud, M., Carlson, M. et al. Vascular biomechanical properties in mice with smooth muscle specific deletion of Ndst1. Mol Cell Biochem 385, 225–238 (2014). https://doi.org/10.1007/s11010-013-1831-3

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