Summary
This report deals with a histochemical, electron microscopic and chemical study of carbohydrates of the guinea pig kidney. Histochemical reactions were positive for glycoproteic components in the lumenal surface of the proximal convoluted tubules as well as in the basal aspects of tubular cells. Mucosubstances containing carboxyl and sulphate groups and sialic acid were present at the cell surfaces of the Bowman's capsule, proximal and distal convoluted tubules, loop of Henle and, in greater concentrations, at the cell surfaces of the collecting tubules and papillary epithelium. Data seemed to indicate that glycosaminoglycans were present at the cell surfaces of the collecting tubules.
By electron microscopy, the alcian blue stained a dense coat of granular appearance at the lumenal surface of the loop of Henle, measuring up to 100 nm in thickness. Ruthenium red stained the glycocalyx of the collecting tubules which measured up to 60 nm in thickness. The glycocalyx of the collecting tubules was made up of thin filaments and a dense component of about 10 nm thick which merged with the outer leaflet of lumenal plasma membrane, which thus appeared asymmetric. With Alcian blue, the glycocalyx of the collecting tubules, which measured 600 to 1500 nm in thickness, was made up of a meshwork of filaments containing globular or irregular structures measuring 50 to 150 nm in diameter. Chemical assays of kidney homogenates revealed carbohydrate components and sulphate of glycosaminoglycans.
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References
Antonopoulos, C. A.: A modification of the determination of sulphate in mucopolysaccharides by the benzidine method. Acta chem. scand. 16, 1521–1522 (1962)
Benassi, G., Zini, F.: Distribuzione di sostanza metachromatiche e ossidabile con acido periodico nei reni di alcuni specie animali. Arch. Sci. Biol. Bologna 35, 377–382 (1951)
Butt, A. J., Hauser, E. A.: The importance of protective urinary colloids in the prevention and treatment of kidney stones. Science 115, 308–310 (1952)
Dische, Z.: A new specific color reaction of hexuronic acids. J. biol. Chem. 167, 189–198 (1947)
Dische, Z.: New color reactions for determination of sugars in polysaccharides. Methods of Biochem. Analysis 2, 313–358 (1955)
Dische, Z., Shettles, L. B.: A specific color reaction of methylpentoses and a spectrophotometric micromethod for their determination. J. biol. Chem. 175, 595–603 (1948)
Drenckhahn, F. O., Meissner, J.: Autoradiographische Untersuchungen über die Lokalisation von Na2 35SO4 in der Meerschweinchenniere. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 227, 444–455 (1956)
Elson, L. A., Morgan, W. T. J.: A colorimetric method for the determination of glucosamine and chondrosamine. Biochem. J. 27, 1824–1828 (1933)
Fischer, E. R., Lillie, R. D.: The effect of methylation on basophilia. J. Histochem. Cytochem. 2, 81–87 (1954)
Fletcher, A. P., McLaughlin, J. E., Ratcliffe, W. A., Woods, D. A.: The chemical composition and electron microscopic appearance of a protein derived from urinary casts. Biochem. biophys. Acta (Amst.) 214, 299–308 (1970)
Francois, C., Marshal, R. D., Neuberger, A.: Carbohydrates in protein. 4. The determination on mannose in hen's-egg albumin by radioisotope dilution. Biochem. J. 83, 335–341 (1962)
Groniowski, J., Biczyskowa, W., Walski, M.: Electron microscope studies on the surface coat of the nephron. J. Cell Biol. 40, 585–601 (1969)
Kantor, T. G., Schubert, M.: A method for the desulfation of chondroitin sulfate. J. Amer. chem. Soc. 79, 152–154 (1957)
Kraan, J. C., Muir, H.: Determination of glucosamine. Biochem. J. 66, 55p (1957)
Lillie, R. D.: Histopathologic technic and practical histochemistry, 2nd ed., New York: The Blakiston Co. 1954
Longley, J. B., Burtner, J. H., Monis, B.: Mucous substance of excretory organs: a comparative study. Ann. N. Y. Acad. Sci. 106, 493–501 (1963)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Monis, B., Gómez, A., Candiotti, A., Ibáñez, N.: The Tamm-Horsfall glycoprotein and the urinary system, electrophoretic and electron microscopic observations. Life. Sci. part II, 11, 699–708 (1972)
Monis, B., Longley, J. B.: Mucins in the epithelium of renal collecting tubules. Nature (Lond.) 176, 741–742 (1955)
Morard, J. C., Abadie, A., Poirier, M. F.: A study of modification induced by antidiuretic hormone (ADH) in the epithelial polyanions of the renal medullary collecting tubules with special references to cell coat. Rev. Europ. Etudes Clin. et Biol. 17, 765–775 (1972)
Mowry, R. W.: The special value of methods that color both acidic and vicinal hydroxyl groups in the histochemical study of mucins. Ann. N. Y. Acad. Sci. 106, 402–423 (1963)
Olsson, J., Gardell, S.: Isolation and characterization of glycosaminoglycans from human leukocytes and platelets. Biochem. biophys. Acta (Amst.) 141, 348–357 (1967)
Pease, D. C.: Polysaccharides associated with the exterior surface of epithelial cells. Anat. Rec. 154, 400 (1966)
Quintarelli, G., Scott, J. E., Dellovo, M. C.: The chemical and histochemical properties of alcian blue. II. Dye binding of tissue polyanions. Histochem. 4, 86–98 (1964)
Rambourg, A.: An improved silver methenamine technique for the detection of periodic acid-reactive complex carbohydrates with the electron microscope. J. Histochem. Cytochem. 15, 409–412 (1967)
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)
Rodriguez, E. M.: Fixation of the central nervous system by perfusion of the cerebral ventricles with a threefold aldehyde mixture. Brain Res. 15, 395–412 (1969)
Scott, J. E.: Aliphatic ammonium salts in the assay of acidic polysaccarides from tissues. Methods of Biochem. Analysis 8, 145–197 (1960)
Scott, J. E., Dorling, J.: Periodate oxidation of acid polysaccharides. III. A PAS method for chondroitin sulphates and other glycosaminoglycuronans. Histochemie 19, 295–301 (1969)
Scott, J. E., Quintarelli, G., Dellovo, M. C.: The chemical and histochemical properties of alcian blue. I. The mechanism of alcian blue staining. Histochemie 4, 73–85 (1964)
Spicer, S. S., Horn, R. G., Leppi, T. J.: Connective Tissues. Internat. Acad. of Path. Monograph No 7, Baltimore (1967)
Spicer, S. S., Jarrels, M. H.: Histochemical reaction of an aromatic diamine with acid groups and periodate engendered aldehydes in mucopolysaccharides. J. Histochem. Cytochem. 9, 368–379 (1961)
Spicer, S. S., Lillie, R. D.: Saponification as a means of selectively reversing the methylation blockade of tissue basophilia. J. Histochem. Cytochem. 7, 123–125 (1959)
Spicer, S. S., Meyer, D. B.: Histochemical differentiation of acid mucopolysaccharides by means of combined aldehyde fuchsin-alcian blue staining. Amer. clin. Path. 33, 453–460 (1960)
Spicer, S. S., Warren, L.: The histochemistry of sialic acid containing mucoproteins. J. Histochem. Cytochem. 8, 135–137 (1960)
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Rovasio, R.A., Lis, D. & Monis, B. Histochemistry and ultrastructure of the cell surfaces of the guinea pig kidney with quantitative data on carbohydrate components of glycosaminoglycans of kidney. Histochemistry 40, 241–251 (1974). https://doi.org/10.1007/BF00501959
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DOI: https://doi.org/10.1007/BF00501959