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Mutagenic effect of N-Methyl-N-nitrosourea onMycobacterium phlei

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Abstract

N-Methyl-N-nitrosourea was used to induce auxotrophic, scotochromogenic and isonicotinic acid hydrazide resistant mutants inMycobacterium phlei and its effect was compared with that of nitrosoguanidine. Seventeen auxotrophic mutants requiring arnino acids or vitamins and 52 sootochromosgenic mutants with orange colonies were induced. The frequency of isonicotinic acid hydrazide-resistant mutants increased by two orders of magnitude.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20, Prague 4

    J. Koníček & M. Koníčková-Radochová

  2. Department of Chemical Mutagenesis, Institute of Biochemistry, Academy of Sciences of the Georgian SSR, 380 031, Tbilisi, USSR

    G. Y. Daraselia

Authors
  1. J. Koníček
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  2. G. Y. Daraselia
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  3. M. Koníčková-Radochová
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The authors thank Mr. V. Košťál for his excellent technical assistance.

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Koníček, J., Daraselia, G.Y. & Koníčková-Radochová, M. Mutagenic effect of N-Methyl-N-nitrosourea onMycobacterium phlei . Folia Microbiol 26, 62–64 (1981). https://doi.org/10.1007/BF02927225

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  • DOI: https://doi.org/10.1007/BF02927225

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