Abstract—
The seasonal dynamics of the structure of microbial biomass in a soddy-podzolic soil under fallow was assessed using luminescent microscopy. Samples from three soil horizons (P, 5‒15 cm, BEL, 30‒40 cm, and BT2, 50‒60 cm) were sampled monthly from March, 2017 to February, 2018, in the territory of Eldigino experimental station (Moscow oblast). In addition to microbial biomass measurement, soil temperature and moisture were recorded. The microbial biomass at all sampling times was dominated by fungi (up to 93%). Minimal microbial population and biomass were observed in the period from November to March. The biomass of prokaryotes increased twofold in May, the maximal values were observed in August and September. The length of actinomycete mycelium was maximal in July and August, when the soil water content was the lowest. Maximal fungal biomass was observed in July and September. Seasonal changes of microbial biomass were most pronounced in the upper soil horizon P, while they were more even in the BEL and BT horizons. Using regression analysis, we revealed a significant effect of temperature and sampling depth on the fungal and prokaryotic biomass. The results indicate substantial seasonal variations in biomass of soil microbiota, which should be taken into account when comparing soils sampled at different seasons.
Similar content being viewed by others
REFERENCES
A. Agnelli, J. Ascher, G. Corti, M. T. Ceccherini, P. Nannipieri, and G. Pietramellara, “Distribution of microbial communities in a forest soil profile investigated by microbial biomass, soil respiration and DGGE of total and extracellular DNA,” Soil Biol. Biochem. 36 (5), 859–868 (2004). https://doi.org/10.1016/j.soilbio.2004.02.004
N. D. Ananyeva, L. M. Polyanskaya, E. A. Susyan, I. V. Vasenkina, S. Wirth, and D. G. Zvyagintsev, “Comparative assessment of soil microbial biomass determined by the methods of direct microscopy and substrate-induced respiration,” Microbiology (Moscow) 77, 356–364 (2008).
N. D. Ananyeva, E. A. Susyan, O. V. Chernova, I. Yu. Chernov, and O. L. Makarova, “The ratio of fungi and bacteria in the biomass of different types of soil determined by selective inhibition,” Microbiology (Moscow) 75, 702–707 (2006).
Yu. A. Vinogradova, E. M. Lapteva, E. M. Perminova, S. S. Anisimov, and A. B. Novakovskii, “Microbial communities of podzolic soils on forest loggings in middle taiga,” Izv. Samar. Nauchn. Tsentra, Ross. Akad. Nauk 16 (5), 74–80 (2014).
A. V. Golovchenko and L. M. Polyanskaya, “Seasonal dynamics of population and biomass of microorganisms in the soil profile,” Eurasian Soil Sci. 29, 1145–1150 (1996).
A. D. Zhelznova, O. V. Kutovaya, V. N. Dmitrenko, A. K. Tkhakakhova, and S. F. Khokhlov, “Evaluation of DNA quantity of various groups of microorganisms in genetic horizons of dark gray soils,” Byull. Pochv. Inst. im. V.V. Dokuchaeva, No. 78, 87–98 (2015).
D. G. Zvyagintsev, Practical Manual on Soil Microbiology and Biochemistry (Moscow, 1991) [in Russian].
L. L. Shishov, V. D. Tonkonogov, I. I. Lebedeva, and M. I. Gerasimova, Classification and Diagnostic System of Russian Soils (Oikumena, Smolensk, 2004) [in Russian].
O. E. Marfenina, D. A. Nikitin, and A. E. Ivanova, “The structure of fungal biomass and diversity of cultivated micromycetes in Antarctic soils (Progress and Russkaya Stations),” Eurasian Soil Sci. 49, 934–941 (2016). https://doi.org/10.1134/S106422931608007X
L. M. Polyanskaya, V. V. Geidebrekht, and D. G. Zvyagintsev, “Fungal biomass in various soil types,” Pochvovedenie, No. 5, 566–572 (1995).
L. M. Polyanskaya and D. G. Zvyagintsev, “The content and composition of microbial biomass as an index of the ecological status of soil,” Eurasian Soil Sci. 38, 625–633 (2005).
V. M. Semenov and B. M. Kogut, Soil Organic Matter (GEOS, Moscow, 2015) [in Russian].
M. V. Semenov, N. A. Manucharova, and A. L. Stepanov, “Distribution of metabolically active prokaryotes (Archaea and Bacteria) throughout the profiles of chernozem and brown semidesert soil,” Eurasian Soil Sci. 49, 217–225 (2016). https://doi.org/10.1134/S1064229316020101
M. V. Semenov, E. V. Stolnikova, N. D. Ananyeva, and K. V. Ivashchenko, “Structure of the microbial community in soil catena of the right bank of the Oka River,” Biol. Bull. (Moscow) 40, 266–274 (2013).
E. B. Skvortsova, V. A. Rozhkov, K. N. Abrosimov, K. A. Romanenko, S. F. Khokhlov, D. D. Khaidapova, V. V. Klyueva, and A. V. Yudina, “Microtomographic analysis of pore space in a virgin soddy-podzolic soil,” Eurasian Soil Sci. 49, 1250–1258 (2016). https://doi.org/10.1134/S1064229316110090
E. A. Susyan, N. D. Ananyeva, and E. V. Blagodatskaya, “The antibiotic-aided distinguishing of fungal and bacterial substrate-induced respiration in various soil ecosystems,” Microbiology (Moscow) 74, 336–342 (2005).
F. M. Khabibullina, E. G. Kuznetsova, and I. Z. Vaseneva, “Micromycetes in podzolic and bog-podzolic soils in the middle taiga subzone of northeastern European Russia,” Eurasian Soil Sci. 47, 1027–1032 (2014). https://doi.org/10.1134/S1064229314100044
E. V. Shein, E. B. Skvortsova, A. V. Dembovetskii, K. N. Abrosimov, L. I. Il’in, and N. A. Shnyrev, “Pore-size distribution in loamy soils: A comparison between microtomographic and capillarimetric determination methods,” Eurasian Soil Sci. 49, 315–325 (2016). https://doi.org/10.1134/S1064229316030091
M. S. Aulakh, R. Wassmann, C. Bueno, J. Kreuzwieser, and H. Rennenberg, “Characterization of root exudates at different growth stages of ten rice (Oryza sativa L.) cultivars,” Plant Biol. 3 (2), 139–148 (2001). https://doi.org/10.1055/s-2001-12905
G. D. Bending, M. K. Turner, F. Rayns, M. C. Marx, and M. Wood, “Microbial and biochemical soil quality indicators and their potential for differentiating areas under contrasting agricultural management regimes,” Soil Biol. Biochem. 36 (11), 1785–1792 (2004). https://doi.org/10.1016/j.soilbio.2004.04.035
J. Bloem, “Microscopic methods for counting bacteria and fungi in soil,” in Methods in Applied Soil Microbiology and Biochemistry (Academic, New York, 1995), pp. 162–173.
N. Dechassa and M. K. Schenk, “Exudation of organic anions by roots of cabbage, carrot, and potato as influenced by environmental factors and plant age,” J. Plant Nutr. Soil Sci. 167 (5), 623–629 (2004). https://doi.org/10.1002/jpln.200420424
D. Epron, V. Le Dantec, E. Dufrene, and A. Granier, “Seasonal dynamics of soil carbon dioxide efflux and simulated rhizosphere respiration in a beech forest,” Tree Physiol. 21 (2–3), 145–152 (2001). https://doi.org/10.1093/treephys/21.2-3.145
A. J. Franzluebbers, F. M. Hons, and D. A. Zuberer, “Tillage and crop effects on seasonal soil carbon and nitrogen dynamics,” Soil Sci. Soc. Am. J. 59 (6), 1618–1624 (1995). https://doi.org/10.2136/sssaj1995.03615995005900060016x
H. Habashi, “Effect of forest and soil type on microbial biomass carbon and respiration,” Eurasian Soil Sci. 49, 1084–1089 (2016). https://doi.org/10.1134/S1064229316090064
K. Isobe, H. Oka, T. Watanabe, R. Tateno, R. Urakawa, C. Liang, K. Senoo, and H. Shibata, “High soil microbial activity in the winter season enhances nitrogen cycling in a cool-temperate deciduous forest,” Soil Biol. Biochem. 124, 90–100 (2018). https://doi.org/10.1016/j.soilbio.2018.05.028
R. G. Joergensen and F. Wichern, “Quantitative assessment of the fungal contribution to microbial tissue in soil,” Soil Biol. Biochem. 40 (12), 2977–2991 (2008). https://doi.org/10.1016/j.soilbio.2008.08.017
K. S. Khan, R. Mack, X. Castillo, M. Kaiser, and R. G. Joergensen, “Microbial biomass, fungal and bacterial residues, and their relationships to the soil organic matter C/N/P/S ratios,” Geoderma 271, 115–123 (2016). https://doi.org/10.1016/j.geoderma.2016.02.019
S. Kramer, S. Marhan, H. Haslwimmer, L. Ruess, and E. Kandeler, “Temporal variation in surface and subsoil abundance and function of the soil microbial community in an arable soil,” Soil Biol. Biochem. 61, 76–85 (2013). https://doi.org/10.1016/j.soilbio.2013.02.006
R. Kuhnert, V. Oberkofler, and U. Peintner, “Fungal growth and biomass development is boosted by plants in snow-covered soil,” Microb. Ecol. 64 (1), 79–90 (2012). https://doi.org/10.1007/s00248-011-0001-y
J. P. van Leeuwen, V. Djukic, J. Bloem, T. Lehtinen, L. Hemerik, P. C. Ruiter de, and G. J. Lair, “Effects of land use on soil microbial biomass, activity and community structure at different soil depths in the Danube floodplain,” Eur. J. Soil Biol. 79, 14–20 (2017). https://doi.org/10.1016/j.ejsobV.2017.02.001
M. Lesiv, D. Schepaschenko, E. Moltchanova, R. Bun, M. Durauer, A. V. Prishchepov, F. Schierhorn, S. Estel, T. Kuemmerle, C. Alcántara, N. Kussul, M. Shchepashchenko, O. Kutovaya, O. Martynenko, V. Karminov, et al., “Spatial distribution of arable and abandoned land across former Soviet Union countries,” Sci. Data 5, 1–12 (2018). https://doi.org/10.1038/sdata.2018.56
M. V. Semenov, T. V. Chernov, A. K. Tkhakakhova, A. D. Zhelezova, E. A. Ivanova, T. V. Kolganova, and O. V. Kutovaya, “Distribution of prokaryotic communities throughout the Chernozem profiles under different land uses for over a century,” Apll. Soil Ecol. 127, 8–18 (2018). https://doi.org/10.1016/j.apsoil.2018.03.002
M. S. Strickland and J. Rousk, “Considering fungal: bacterial dominance in soils—Methods, controls, and ecosystem implications,” Soil Biol. Biochem. 42 (9), 1385–1395 (2010). https://doi.org/10.1016/j.soilbio.2010.05.007
F. T. Vries, E. Hoffland, N. van Eekeren, L. Brussaard, and J. Bloem, “Fungal/bacterial ratios in grasslands with contrasting nitrogen management,” Soil Biol. Biochem. 38 (8), 2092–2103 (2006). https://doi.org/10.1016/j.soilbio.2006.01.008
D. Zhang, X. Sun, G. Zhou, J. Yan, Y. Wang, S. Liu, C. Zhou, J. Liu, X. Tang, J. Li, and Q. Zhang, “Seasonal dynamics of soil CO2 effluxes with responses to environmental factors in lower subtropical forests of China,” Sci. China, Ser. D: Earth Sci. 49 (2), 139–149 (2006). https://doi.org/10.1007/s11430-006-8139-z
ACKNOWLEDGMENTS
This work was supported by the Russian Science Foundation, project no. 17-16-01057.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated by T. Chicheva
Rights and permissions
About this article
Cite this article
Nikitin, D.A., Chernov, T.V., Zhelezova, A.D. et al. Seasonal Dynamics of Microbial Biomass in Soddy-Podzolic Soil. Eurasian Soil Sc. 52, 1414–1421 (2019). https://doi.org/10.1134/S1064229319110073
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1064229319110073