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Seed priming with polyethylene glycol induces antioxidative defense and metabolic regulation of rice under nano-ZnO stress

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Abstract

The present study was carried out to investigate the beneficial impact of seed priming with polyethylene glycol (PEG) under different concentrations of zinc oxide nanoparticles (nano-ZnO), i.e., 0, 250, 500, and 750 mg L−1 in two cultivars of Oryza sativa (Zhu Liang You 06 and Qian You No. 1). Physiological parameters were improved by priming with 30 % PEG in both cultivars under stress treatments. Seed priming with 30 % PEG improved α-amylase activities and total soluble sugar contents of both cultivars under nano-ZnO stress. In addition, glutathione reductase (GR) activity, reactive oxygen species (ROS) accumulation, and proline contents decreased after the priming treatment in both cultivars under different nano-ZnO concentrations. Expression of GR1, GR2, Amy2A, and Amy3A genes in shoots and roots of both cultivars increased and had higher transcription levels under the nano-ZnO stress condition. Fourier transform infrared spectroscopy (FTIR) analysis did not show any significant effects of the priming treatment on the band observed at 3400, 900, 1600, and 1000 cm−1 corresponding to alkenyl stretch (C = C), carboxyl acid (O-H), nitrile (C = N), and aromatic (C-H), respectively, in both cultivars under nano-ZnO stress.

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Acknowledgments

This research was supported by Zhejiang Provincial Natural Science Foundation (LZ14C130002, LY15C130002), the Special Fund for Agro-scientific Research in the Public Interest (no. 201203052), the Project of the Science and Technology Department of Zhejiang Province (no. 2013C02005, 2013C32023), the Fundamental Research Funds for the Central Universities (no. 2015QNA6019), and Jiangsu Collaborative Innovation Center for Modern Crop Production, People’s Republic of China.

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Authors and Affiliations

  1. Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China

    Mohamed Salah Sheteiwy, Yuying Fu, Qijuan Hu, Aamir Nawaz, Yajing Guan, Zhan Li, Yutao Huang & Jin Hu

  2. Department of Agronomy, Faculty of Agriculture, Mansoura University, Mansoura, 35516, Egypt

    Mohamed Salah Sheteiwy

  3. Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, 60000, Pakistan

    Aamir Nawaz

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  1. Mohamed Salah Sheteiwy
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  5. Yajing Guan
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Correspondence to Yajing Guan or Jin Hu.

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Sheteiwy, M.S., Fu, Y., Hu, Q. et al. Seed priming with polyethylene glycol induces antioxidative defense and metabolic regulation of rice under nano-ZnO stress. Environ Sci Pollut Res 23, 19989–20002 (2016). https://doi.org/10.1007/s11356-016-7170-7

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