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In vitro gastro-intestinal method for the assessment of heavy metal bioavailability in contaminated soils

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Abstract

Introduction

Balya and its associated villages which is a town of the Balikesir region of Turkey have very rich zinc, lead, and manganese mines. These mines have been operating since the thirteenth century and now there is heavy metal contamination in both the soil and natural waters in these areas.

Materials and methods

Soils were collected from Sarı su, Enverpaşa, and Hastanetepe which are in Balya town and Kadıköy, Kaşıkcı, Müstecap, Patlak, Çakallar, and Bengiler which are the villages near Balya and the mine areas. Nine trace analytes (As, Ba, Cd, Cr, Cu, Mn, Ni, Pb, and Zn) were determined using an acid extraction procedure as well as from PBET in vitro gastro-intestinal experiments using ICP-OES.

Results and discussion

The results showed that high As, Ba, Pb, Zn, and Cd concentrations were found in these soils. The amounts ingested by pica behavior of children at the rate of 10 g day−1 are calculated using the results of in vitro intestinal bio-accessibility experiments.

Conclusion

The results showed that the amount of As, Pb, Ba, and Cd levels ingested by pica behavior are substantially higher than tolerable daily intake values in most of the soils. When normal ingestion is taken into account, the tolerable daily limits are only exceeded for one element (Pb) and even then, only at two sites.

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References

  • Baars AJ, Theelen RMC, Janssen PJCM, Hesse JM, van Apeldoorn ME, Meijerink MCM, Verdam L, Zeilmaker MJ (2001) Re-evaluation of human-toxicological maximum permissible risk levels. RIVM Report 711701025

  • Calabrese EJ, Stanek EJ (1995) Resolving intertracer inconsistencies in soil ingestion estimation. Environ Health Perspect 103:454–457

    CAS  Google Scholar 

  • Calabrese EJ, Barnes R, Stanek EJ, Pastides H, Gilbert CE, Veneman P, Wang X, Lasztity A, Kostecki PT (1989) How much soil do young children ingest: an epidemiologic study. Reg Tox Pharmacol 10:123–137

    Article  CAS  Google Scholar 

  • Dean JR (2007) Bioavailability, bioaccessibility and mobility of environmental contaminants. Wiley, Chichester, 194

    Book  Google Scholar 

  • Ellis CR, Schnoes CJ (2009) Eating disorder: Pica. < emedicine.medscape.com/article/914765-overview > (accessed 01.05.10)

  • Fanfani L, Zuddas P, Chessa A (1997) Heavy metals speciation analysis as a tool for studying mine tailings weathering. J Geochem Explor 58:241–248

    Article  CAS  Google Scholar 

  • Hamel SC, Buckley B, Lioy PJ (1998) Bioaccessibility of metals in soils for different liquid to solid ratios in synthetic gastric fluid. Environ Sci Technol 32:358–362

    Article  CAS  Google Scholar 

  • Intawongse M, Dean JR (2008) Use of the physiologically-based extraction test to assess the oral bioaccessibility of metals in vegetable plants grown in contaminated soil. Environ Pollut 152–1:60–72

    Article  Google Scholar 

  • Kaçar B (2009) Toprak Analizleri, Nobel Yayınları, Second Edition, 221

  • Miller DD, Schricker BR, Rasmussen RR, Van Campen D (1981) An invitro method for estimation of iron availability from meals. Am J Clin Nutr 34:2248–2256

    CAS  Google Scholar 

  • Oomen AG, Hack A, Minekus M, Zeijdner E, Cornelis C, Schoeters G, Verstraete W, Van De Wiele T, Wragg J, Rompelberg CJM, Sips AJAM, Van Wijnen JH (2002) Comparison of five in vitro digestion models to study the bioaccessibility of soil contaminants. Environ Sci Technol 36:3326–3334

    Article  CAS  Google Scholar 

  • Rodriguez RR, Basta NT, Casteel SW, Pace LW (1999) An in vitro gastrointestinal method to estimate bioavailable arsenic in contaminated soils and solid media. Environ Sci Technol 33:642–649

    Article  CAS  Google Scholar 

  • Ruby MV, Davis A, Link TE, Schoof R, Chaney RL, Freeman GB, Bergstrom P (1993) Development of an in-vitro screening-test to evaluate the in-vivo bioaccessibility of ingested mine-waste lead. Environ Sci Technol 27:2870–2877

    Article  CAS  Google Scholar 

  • Ruby MV, Davis A, Schoof R, Eberle S, Sellstone CM (1996) Estimation of lead and arsenic bioavailability using a physiologically based extraction test. Environ Sci Technol 30:422–430

    Article  CAS  Google Scholar 

  • Ruby MV, Schoof R, Brattin W, Goldade M, Post G, Harnois M, Mosby DE, Casteel SW, Berti W, Carpenter M, Edwards D, Cragin D, Chappell W (1999) Advances in evaluating the oral bioavailability of inorganics in soil for use in human health risk assessment. Environ Sci Technol 33:3697–3705

    Article  CAS  Google Scholar 

  • Schroder J, Basta NT, Si J, Casteel SW, Evans T, Payton M (2003) In vitro gastrointestinal method to estimate relative bioavailable cadmium in contaminated soil. Environ Sci Technol 37:1365–1370

    Article  CAS  Google Scholar 

  • Sutherland RA, Tack FMG (2000) Metal phase associations in soils from an urban watershed, Honolulu, Hawaii. Sci Total Environ 256:103–113

    Article  CAS  Google Scholar 

  • Turkish Environmental and Forestry Ministry (2005) Toprak Kirliliğinin Kontrolü Yönetmeliği, http://www2.cevreorman.gov.tr/yasa/yonetmelik.asp

  • US Environmental Protection Agency (EPA) (2008) Child-Specific Exposure Factors Handbook (Final Report) 2008. U.S. Environmental Protection Agency, Washington, DC, EPA/600/R-06/096F, 2008. Available from: http://cfpub.epa.gov/ncea

    Google Scholar 

  • USDA, Food and Nutrition Information Center (2009) Dietary Reference Intakes, Elements, <http://fnic.nal.usda.gov> (accessed 01.05.2010)

  • Van Wijnen JH, Clausing P, Brunekreef B (1990) Estimated soil ingestion by children. Environ Res 51:147–162

    Article  Google Scholar 

  • Zemberyova M, Bartekova J, Hagarova I (2006) The utilization of modified BCR three-step sequential extraction procedure for the fractionation of Cd, Cr, Cu, Ni, Pb and Zn in soil reference materials of different origins. Talanta 70:973–978

    Article  CAS  Google Scholar 

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Correspondence to Derya Kara.

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Responsible editor: Markus Hecker

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Karadaş, C., Kara, D. In vitro gastro-intestinal method for the assessment of heavy metal bioavailability in contaminated soils. Environ Sci Pollut Res 18, 620–628 (2011). https://doi.org/10.1007/s11356-010-0404-1

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