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Comparison of waterborne and in ovo nanoinjection exposures to assess effects of PFOS on zebrafish embryos

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Abstract

Since perfluorooctane sulfonate (PFOS) had been detected in eggs of seabirds and fish, toxicity of waterborne PFOS to embryonic development of zebrafish (Danio rerio) was investigated. However, because assessment of effects by use of dietary exposure of adults is time-consuming and expensive, a study was conducted to compare effects on embryos via nanoinjection and waterborne exposure. Nanoinjection, in which small amounts of chemicals are injected into developing eggs, was used to incorporate PFOS into the yolk sac of embryos of zebrafish. Effects of PFOS during the period of development of the embryo were assessed within 96 h post-fertilization (hpf). PFOS significantly retarded development of embryos of zebrafish and resulted in abnormalities as well as lethality of developing embryos. Both methods of exposure, waterborne and nanoinjection, resulted in similar dose-dependent effects. Some sublethal effects, such as edema at 48 hpf, delayed hatching, and curvature of the spine was observed after 72 hpf. In ovo, nanoinjection was deemed to be an accurate method of exposure for controlling the actual internal dose for study of adverse effects, which closely mimicked responses to waterborne exposure of zebrafish embryo to PFOS.

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Acknowledgments

This work was jointly funded by the National Natural Science Foundation of China (No. 21377053, 20977047), Major National Science and Technology Projects (No. 2012ZX07506-001, 2012ZX07501-003-02). Prof. Giesy was supported by the program of 2012 “High Level Foreign Experts” (GDW20123200120) funded by the State Administration of Foreign Experts Affairs, the P.R. China to Nanjing University and the Einstein Professor Program of the Chinese Academy of Sciences. He was also supported by the Canada Research Chair program, a Visiting Distinguished Professorship in the Department of Biology and Chemistry and State Key Laboratory in Marine Pollution, City University of Hong Kong.

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Authors and Affiliations

  1. State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China

    Yabing Li, Zhihua Han, Xinmei Zheng, Zhiyuan Ma, Hongling Liu, John P. Giesy, Yuwei Xie & Hongxia Yu

  2. Ministry Environment Protection China, Nanjing Institute Environment Science, Nanjing, 210042, China

    Zhihua Han

  3. Department of Biomedical and Veterinary Biosciences and Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

    John P. Giesy

  4. Department of Biology and Chemistry, State Key Laboratory for Marine Pollution, City University of Hong Kong, Kowloon, Hong Kong, SAR, China

    John P. Giesy

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  1. Yabing Li
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  2. Zhihua Han
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  3. Xinmei Zheng
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  4. Zhiyuan Ma
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  5. Hongling Liu
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  6. John P. Giesy
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  7. Yuwei Xie
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Correspondence to Hongling Liu or Hongxia Yu.

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Responsible editor: Philippe Garrigues

Yabing Li and Zhihua Han contributed equally to this work.

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Li, Y., Han, Z., Zheng, X. et al. Comparison of waterborne and in ovo nanoinjection exposures to assess effects of PFOS on zebrafish embryos. Environ Sci Pollut Res 22, 2303–2310 (2015). https://doi.org/10.1007/s11356-014-3527-y

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  • DOI: https://doi.org/10.1007/s11356-014-3527-y

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