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Effect of the Mammalian Arginase Inhibitor 2(S)-Amino-6-Boronohexanoic Acid on Bacillus anthracis Arginase

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Abstract

Macrophages, upon phagocytosing endospores of Bacillus anthracis, up-regulate the expression of the immunological isoform of nitric oxide synthase, NOS 2, concomitant with production of nitric oxide (NO•) from metabolism of l-arginine. We have previously demonstrated that macrophages that secrete NO• kill the bacilli of B. anthracis. To circumvent this microbicidal activity of NO•, B. anthracis has evolved pathways that include the enzyme arginase, which metabolizes l-arginine to ornithine and urea. Compounds that inhibit arginase might, therefore, offer a therapeutic approach to controlling B. anthracis infection. 2(S)-Amino-6-boronohexanoic acid (ABH) has been reported to be an inhibitor of mammalian arginase. In this study, we explore the inhibitory effect of ABH against B. anthracis arginase and its potential for future development, as an effective therapeutic agent against microbial infection. We found that ABH is an inhibitor of bacterial arginase in several different endospore strains of B. anthracis. Further, ABH inhibits neither the phagocytosis of these endospores nor the up-regulation of NOS 2 concomitant with secretion of NO•. These findings set the stage to determine how efficacious ABH will be in promoting NO•-mediating killing of B. anthracis.

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Acknowledgment

This research was supported in part by a Grant from the Maryland Industrial Partnership and Corridor Pharmaceuticals, Inc.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, 20 North Pine St., Baltimore, MD, 21201, USA

    Pei Tsai, Guan-Liang Cao, Paul Shapiro & Gerald M. Rosen

  2. Center for EPR Imaging In Vivo Physiology, University of Maryland, Baltimore, MD, 21201, USA

    Pei Tsai, Guan-Liang Cao & Gerald M. Rosen

  3. Corridor Pharmaceuticals, Inc, Baltimore, MD, 21093, USA

    Bruce Tomczuk & Peter D. Suzdak

  4. Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, 21201, USA

    Alan S. Cross

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  1. Pei Tsai
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  2. Guan-Liang Cao
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  3. Bruce Tomczuk
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  4. Peter D. Suzdak
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  5. Alan S. Cross
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  6. Paul Shapiro
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  7. Gerald M. Rosen
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Correspondence to Gerald M. Rosen.

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Tsai, P., Cao, GL., Tomczuk, B. et al. Effect of the Mammalian Arginase Inhibitor 2(S)-Amino-6-Boronohexanoic Acid on Bacillus anthracis Arginase. Curr Microbiol 64, 379–384 (2012). https://doi.org/10.1007/s00284-012-0084-9

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  • DOI: https://doi.org/10.1007/s00284-012-0084-9

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