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Development of Graphene Oxide Functionalized Cotton Fiber Based Solid Phase Extraction Combined with Liquid Chromatography-Fluorescence Detection for Determination of Trace Auxins in Plant Samples

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Abstract

In this work, a convenient and sensitive solid phase extraction (SPE) method was developed based on graphene oxide functionalized cotton fiber (GO-CF), a SPE sorbent followed by liquid chromatography-fluorescence detection (LC-FLD) for determination of trace indole acetic acid, indole propionic acid, indole butyric acid and naphthalene acetic acid. GO-CF was synthesized via covalent bonding method on the surface of cotton fiber, and exhibited higher extraction capacity in comparison with that of cotton fiber. The conditions affecting the extraction efficiency, including sample pH, elution solvent, elution volume, extraction time, and sample volume, were optimized to obtain high extraction recoveries. Under optimal conditions, the calibration curves were linear over the concentration ranging from 2 to 200 ng mL−1 with correlation coefficients between 0.9987 and 0.9998 for all the analytes. The limits of detection were lower than 0.371 ng mL−1 for the four auxins. The intra- and inter-day relative standard deviations (RSDs) were less than 3.5 and 7.1%, respectively. The mean recoveries ranged from 73.5 to 105.4% with RSDs lower than 12.6%. The developed GO-CF–SPE–LC-FLD method has been successfully applied to the analysis of four auxins in real plant samples, including mung bean stalk, mung bean sprout, and tobacco leaf samples.

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Acknowledgements

This study was funded by the National Natural Science Foundation of China (Grant nos. 21465008, 21265004, and 21665006), the Natural Science Foundation from Guangxi Zhuang Autonomous Region (nos. 2015GXNSFAA139024 and 2015GXNSFFA139005), the Project of High Level Innovation Team and Outstanding Scholar in Guangxi Colleges and Universities (Guijiaoren[2014]49), and the Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection.

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  1. Qiulian Zeng and Yuji Ruan contributed equally to this work.

Authors and Affiliations

  1. Guangxi Key Laboratory of Electrochemical and Magnetochemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China

    Qiulian Zeng, Lingshun Sun, Fuyou Du, Lin Guo, Zhenfang Cheng, Guihua Ruan & Jianping Li

  2. School of Biomedical Engineering, Southern Medical University, Guangzhou, 510515, China

    Yuji Ruan

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  1. Qiulian Zeng
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  3. Lingshun Sun
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  4. Fuyou Du
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  6. Zhenfang Cheng
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Correspondence to Fuyou Du or Guihua Ruan.

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Zeng, Q., Ruan, Y., Sun, L. et al. Development of Graphene Oxide Functionalized Cotton Fiber Based Solid Phase Extraction Combined with Liquid Chromatography-Fluorescence Detection for Determination of Trace Auxins in Plant Samples. Chromatographia 81, 861–869 (2018). https://doi.org/10.1007/s10337-018-3518-0

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  • DOI: https://doi.org/10.1007/s10337-018-3518-0

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