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Simultaneous Determination of Neuroactive Amino Acids in Serum by CZE Coupled with Amperometric Detection

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Abstract

A quantitative determination of six neuroactive amino acids (NAAs) was performed by capillary zone electrophoresis with amperometric detection (CZE-AD). This CZE-AD method utilized two electrolytes: the borate solution flowing in a capillary has the NAAs-separation effects, and the sodium hydroxide (NaOH) solution filled in the detection reservoir for the amperometric analysis of NAAs. The following experimental parameters were optimized: the working electrode potential, the pH value, the component, and the concentration of running buffer, the separation voltage, and the injection time on CZE-AD. Then, under the optimum conditions, the six NAAs could be completely separated in 30 min and had well-shaped AD responses at 0.75 V (versus SCE) on a copper electrode. The linear calibration range of NAAs was from 5 × 10−4 to 5 × 10−6 mol L−1 with the limits of detection (LODs) ranging from 10−6 to 10−7 mol L−1 (signal-to-noise ratio = 3), and the relative standard deviations (RSDs) of the migration time and peak area were 0.45–0.55 and 3.8–6.3 %, respectively. Moreover, this method has succeeded in human serum analysis, and the determined contents of the six NAAs in human serum were in an average recovery range of 85.3–117.9 %, which confirmed the validity and practicability of this method.

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Acknowledgments

This work was financially supported by the Program New Century Excellent Talents in University (Grant NCET-08-0191) and the National Program on the Development of Scientific Instrument and Equipment (Grant 2011YQ150072).

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Authors and Affiliations

  1. Department of Chemistry, East China Normal University, Shanghai, 200241, People’s Republic of China

    Shuli Ge, Huan Wang, Zhifang Wang, Shuang Cheng, Qingjiang Wang, Pingang He & Yuzhi Fang

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  1. Shuli Ge
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  2. Huan Wang
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  3. Zhifang Wang
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  5. Qingjiang Wang
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Correspondence to Qingjiang Wang.

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Ge, S., Wang, H., Wang, Z. et al. Simultaneous Determination of Neuroactive Amino Acids in Serum by CZE Coupled with Amperometric Detection. Chromatographia 76, 149–155 (2013). https://doi.org/10.1007/s10337-012-2378-2

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