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Fabrication and Characterization of a Dye-Immobilized Yttria-Stabilized Zirconia Pellicular Adsorbent for Expanded Bed Adsorption Chromatography

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Abstract

This study deals with the fabrication and characterization of a pellicular adsorbent appropriate for the expanded bed adsorption (EBA) process. The synthesized adsorbent has an yttria-stabilized zirconia nucleus coated with agarose. Morphological analysis of the coated particles was performed by light-scattering microscopy and showed an average diameter of 197.54 and 202.25 µm, for the nucleus and coated particle, respectively. A screening for the reactive dyes reactive blue 19 (RB19), reactive blue 21 (RB21) and reactive orange 107 (RO107) was performed after immobilization onto the pellicular adsorbent by changing the pH, aiming at finding the binding capacity of these to adsorb bovine serum albumin (BSA). The reactive orange 107 was selected and it was more stable at pH 4.5. Study of the kinetics between BSA and the dye-immobilized particle showed that equilibrium is reached before 1 h. The adsorption isotherm of BSA onto RO107-immobilized adsorbent fitted the Langmuir model showing a qm = 102.328 mg BSA/mL of adsorbent. The pellicular adsorbent also showed good expansion even at a high operating flow rate. Therefore, at a linear velocity as high as 2725 cm/h, a dynamic capacity of 15.7 mg of BSA/mL of adsorbent was obtained.

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Acknowledgements

The authors thank the CAPES and CNPq for the financial support.

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Authors and Affiliations

  1. Department of Chemical Engineering, Technology Center, Federal University of Rio Grande do Norte, Natal, CEP 59072-970, Brazil

    Cinthia Meirelly de Araújo Elpídio, Carlos Eduardo de Araújo Padilha, Francisco Canindé de Sousa Júnior, Ruthinéia Jéssica Alves do Nascimento, Camilla Milena de Araújo Elpídio, Sérgio Dantas de Oliveira Júnior, Gorete Ribeiro de Macedo & Everaldo Silvino dos Santos

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  1. Cinthia Meirelly de Araújo Elpídio
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  2. Carlos Eduardo de Araújo Padilha
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Correspondence to Everaldo Silvino dos Santos.

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de Araújo Elpídio, C.M., de Araújo Padilha, C.E., de Sousa Júnior, F.C. et al. Fabrication and Characterization of a Dye-Immobilized Yttria-Stabilized Zirconia Pellicular Adsorbent for Expanded Bed Adsorption Chromatography. Chromatographia 81, 1355–1364 (2018). https://doi.org/10.1007/s10337-018-3581-6

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