Abstract
DNA sequence divergence was analyzed in some sibling species of the Drosophila virilis group. Clones comprising about 0.1% of the genome DNA were selected at random from a D. virilis library for a comparative study on DNA from D. lummei, D. novamexicana, D. borealis, and D. lacicola. Blot hybridization experiments indicated that about 70% of DNA from D. lummei and D. novamexicana and less than 50% of DNA from D. borealis and D. lacicola share sequences that are homologous to DNA in D. virilis. This finding is in excellent agreement with the genealogical tree based on cytological studies (Throckmorton 1982). — Four plasmids with inserts which are present in one or a few copies per genome were hybridized in situ to polytene chromosomes. These experiments demonstrate that (1) homologous “unique” DNA sequences are localized exclusively in homologous bands and (2) homologous bands that appear to be identical in different species may contain different DNA sequences.
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References
Boyer HW, Roulland-Dussoix D (1969) A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41:459–472
Cohen EH, Rae PMM, Tsai L-J (1980) Relatedness of rDNA intervening sequences among species of Drosophila. J Cell Biol 87,1:D112a
Denhardt DT (1966) A membrane filter technique for the detection of complementary DNA. Biochem Biophys Res Commun 23:641–646
Hanahan D, Meselson M (1980) Plasmid screening at high colony density. Gene 10:63–67
Hollenberg CP (1976) Proportionate representation of rDNA and Balbiani ring DNA in polytene chromosomes of Chironomus tentans. Chromosoma 57:185–197
Hsu TC (1952) Chromosomal variation and evolution in the virilis group of Drosophila. In: Patternson (ed) Studies in the genetics of Drosophila 7:60–65
Jones CW, Rosenthal N, Rodaleis GC, Kafatos FC (1979) Evolution of two major chorion multigene families as inferred from cloned cDNA and protein sequences. Cell 18:1317–1322
Katz LR, Kingsbury DT, Helinsky DR (1973) Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex. J Bacteriol 114:577–591
Kress H (1972) Das Puffmuster der Riesenchromosomen in den larvalen Speicheldrüsen von Drosophila virilis. Seine Veränderung in der Normalentwicklung und nach Injektion von Ecdyson. Verl. Bayer. Akad. Wissensch., München
Laird CD, McCarthy BJ (1969) Molecular characterization of the Drosophila genome. Genetics 63:865–882
Lennox ES (1955) Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1:190–206
Maniatis T, Kee SK, Efstratiadis A, Kafatos FC (1976) Amplitification and characterization of a β-globin gene synthesized in vitro. Cell 8:163–182
Pardue ML, Gall JG (1975) Nucleic acid hybridization to the DNA of cytological preparations. Methods in Cell Biol 10:1–16
Patterson JT, Stone WS (1952) Evolution in the genus Drosophila. Macmillan Co, New York
Rae PMM (1970) Chromosomal distribution of rapidly reannealing DNA in Drosophila melanogaster. Proc Natl Acad Sci 67:1018–1025
Rae PMM, Franke WW (1972) The interphase distribution of satellite DNA containing heterochromatin in mouse nuclei. Chromosoma 39:433–456
Sorsa V, Green MM, Beermann W (1973) Cytogenetic fine structure and chromosomal localization of the white gene in Drosophila melanogaster. Nat New Biol 245:34–37
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Sussenbach JS, Monfoort CH, Schiphof R, Stobberingh EE (1976) A restriction endonuclease from Staphylococcus aureus. Nucl Acids Res 3:3193–3202
Sutcliffe JG (1978) pBR 322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4362 nucleotide pairs long. Nucl Acids Res 5:2721–2728
Throckmorton LH (1982) The virilis species group. In: Ashburner M, Carson HL, Thompson JN Jr (eds) The Genetics and Biology of Drosophila, vol 3b. Adademic Press, New York, pp 227–296
Wahl GM, Stern M, Stark GR (1979) Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci 76:3683–3687
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Riede, I., Jacob, E. & Renz, M. DNA sequence divergence in the Drosophila virilis group. Chromosoma 88, 109–115 (1983). https://doi.org/10.1007/BF00327330
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DOI: https://doi.org/10.1007/BF00327330