Abstract
Many of the cell fate decisions in precursor B cells and more mature B cells are controlled by membrane immunoglobulin (Ig)M heavy chain (mu) and the Ig alpha-Ig beta signal transducers. The role of Ig beta in regulating early B cell development was examined in mice that lack Ig beta (Ig beta-/-). These mice had a complete block in B cell development at the immature CD43+B220+ stage. Immunoglobulin heavy chain diversity (DH) and joining (JH) segments rearranged, but variable (VH) to DJH recombination and immunoglobulin messenger RNA expression were compromised. These experiments define an unexpected, early requirement for Ig(beta) to produce B cells that can complete VDJH recombination.
MeSH terms
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Animals
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Antigens, CD / genetics
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Antigens, CD / physiology*
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B-Lymphocytes / cytology
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B-Lymphocytes / immunology*
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CD79 Antigens
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Gene Expression
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Gene Rearrangement, B-Lymphocyte, Heavy Chain*
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Gene Targeting
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Genes, Immunoglobulin
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Immunoglobulin Heavy Chains / genetics*
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Immunoglobulin Joining Region / genetics
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Immunoglobulin Light Chains / genetics*
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Immunoglobulin Variable Region / genetics
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Immunoglobulin mu-Chains / biosynthesis
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Immunoglobulin mu-Chains / genetics
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Immunoglobulin mu-Chains / physiology
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Lymph Nodes
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Mice
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Mice, Inbred C57BL
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Mutation
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RNA, Messenger / genetics
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Receptors, Antigen, B-Cell / physiology
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Recombination, Genetic*
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Signal Transduction
Substances
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Antigens, CD
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CD79 Antigens
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Cd79a protein, mouse
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Cd79b protein, mouse
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Immunoglobulin Heavy Chains
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Immunoglobulin Joining Region
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Immunoglobulin Light Chains
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Immunoglobulin Variable Region
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Immunoglobulin mu-Chains
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RNA, Messenger
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Receptors, Antigen, B-Cell