Abstract
Early stages of T cell development are thought to include a series of coordinated interactions between thymocytes and other cells of the thymus. A monoclonal antibody specific for mouse CD81 was identified that blocked the appearance of alpha beta but not gamma delta T cells in fetal organ cultures initiated with day 14.5 thymus lobes. In reaggregation cultures with CD81-transfected fibroblasts, CD4-CD8- thymocytes differentiated into CD4+CD8+ T cells. Thus, interactions between immature thymocytes and stromal cells expressing CD81 are required and may be sufficient to induce early events associated with T cell development.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Antibodies, Monoclonal / immunology
-
Antigens, CD / genetics
-
Antigens, CD / immunology
-
Antigens, CD / physiology*
-
Base Sequence
-
CD4 Antigens / analysis
-
CD8 Antigens / analysis
-
CHO Cells
-
Cell Differentiation
-
Cricetinae
-
Membrane Proteins / immunology
-
Membrane Proteins / physiology*
-
Mice
-
Molecular Sequence Data
-
Organ Culture Techniques
-
Receptors, Antigen, T-Cell, alpha-beta / analysis*
-
Receptors, Antigen, T-Cell, gamma-delta / analysis
-
Stromal Cells / immunology
-
T-Lymphocyte Subsets / cytology
-
T-Lymphocyte Subsets / immunology*
-
Tetraspanin 28
-
Thymus Gland / cytology
-
Thymus Gland / immunology*
-
Transfection
Substances
-
Antibodies, Monoclonal
-
Antigens, CD
-
CD4 Antigens
-
CD8 Antigens
-
Cd81 protein, mouse
-
Membrane Proteins
-
Receptors, Antigen, T-Cell, alpha-beta
-
Receptors, Antigen, T-Cell, gamma-delta
-
Tetraspanin 28