Abstract
Src homology 2 (SH2) domains bind specifically to tyrosine-phosphorylated proteins that participate in signaling by growth factors and oncogenes. A protein domain was identified that bound specifically to the tyrosine-phosphorylated form of its target protein but differs from known SH2 sequences. Phosphotyrosine-binding (PTB) domains were found in two proteins: SHC, a protein implicated in signaling through Ras; and SCK, encoded by a previously uncharacterized gene. The PTB domain of SHC specifically bound to a tyrosine-phosphorylated 145-kilodalton protein. PTB domains are an alternative to SH2 domains for specifically recruiting tyrosine-phosphorylated proteins into signaling complexes and are likely to take part in signaling by many growth factors.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3T3 Cells
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Adaptor Proteins, Signal Transducing*
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Adaptor Proteins, Vesicular Transport*
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Amino Acid Sequence
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Animals
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Cell Line
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Humans
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Mice
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Molecular Sequence Data
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Phosphoproteins / metabolism*
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Phosphorylation
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Phosphotyrosine
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Platelet-Derived Growth Factor / pharmacology
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Protein Binding
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Protein-Tyrosine Kinases / chemistry
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Protein-Tyrosine Kinases / metabolism
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Proteins / chemistry
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Proteins / metabolism*
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Shc Signaling Adaptor Proteins
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Signal Transduction*
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Src Homology 2 Domain-Containing, Transforming Protein 1
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Tyrosine / analogs & derivatives*
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Tyrosine / metabolism
Substances
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Adaptor Proteins, Signal Transducing
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Adaptor Proteins, Vesicular Transport
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Phosphoproteins
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Platelet-Derived Growth Factor
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Proteins
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SHC1 protein, human
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Shc Signaling Adaptor Proteins
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Shc1 protein, mouse
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Src Homology 2 Domain-Containing, Transforming Protein 1
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Phosphotyrosine
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Tyrosine
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Protein-Tyrosine Kinases