Abstract
We identified a naturally occurring hepatocyte growth factor (HGF) variant, whose predicted sequence extends only through the second kringle domain of this plasminogen-related molecule. This smaller molecule, derived from an alternative HGF transcript, lacked mitogenic activity but specifically inhibited HGF-induced mitogenesis. Cross-linking studies demonstrated that the truncated molecule competes with HGF for binding to the HGF receptor, which has been identified as the c-met protooncogene product. Thus, the same gene encodes both a growth factor and its direct antagonist.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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Cell Line
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Culture Media
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DNA / genetics
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DNA Replication / drug effects
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Epidermal Growth Factor / pharmacology
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Growth Substances / genetics*
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Growth Substances / isolation & purification
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Growth Substances / pharmacology
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Hepatocyte Growth Factor
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Humans
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Kinetics
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Plasmids
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Poly A / genetics
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Poly A / isolation & purification
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Polymerase Chain Reaction / methods
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RNA / genetics
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RNA / isolation & purification
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RNA, Messenger
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Thymidine / metabolism
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Transcription, Genetic*
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Transfection
Substances
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Culture Media
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Growth Substances
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Oligodeoxyribonucleotides
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RNA, Messenger
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Poly A
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Epidermal Growth Factor
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RNA
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Hepatocyte Growth Factor
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DNA
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Thymidine