Abstract
A human intestinal intraepithelial lymphocyte (IEL) T cell line was established from jejunum to characterize the structure and function of the alpha beta T cell antigen receptors (TCRs) expressed by this population. Single-sided polymerase chain reaction (PCR) amplification cloning and quantitative PCR amplification of the TCR chains from the cell line and from fresh IELs demonstrated that IELs were oligoclonal. The IEL T cell line exhibited CD1-specific cytotoxicity and a dominant IEL T cell clone was CD1c-specific. Thus, human jejunal intraepithelial lymphocytes are oligoclonal and recognize members of the CD1 gene family.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Antigens, CD / genetics*
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Antigens, CD / immunology
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Antigens, CD1
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Base Sequence
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Cell Line
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Clone Cells
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Epithelium / physiology
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Humans
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Jejunum / immunology
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Molecular Sequence Data
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Oligonucleotide Probes
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Polymerase Chain Reaction / methods
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Receptors, Antigen, T-Cell / genetics*
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T-Lymphocytes / immunology*
Substances
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Antigens, CD
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Antigens, CD1
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Oligonucleotide Probes
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Receptors, Antigen, T-Cell
Associated data
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GENBANK/M61419
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GENBANK/M61420
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GENBANK/M61421
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GENBANK/M61422
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GENBANK/M61423
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GENBANK/M61424
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GENBANK/M61425
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GENBANK/M61426
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GENBANK/S56162
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GENBANK/S56171