Abstract
Linker proteins function as molecular scaffolds to localize enzymes with substrates. In B cells, B cell linker protein (BLNK) links the B cell receptor (BCR)-activated Syk kinase to the phosphoinositide and mitogen-activated kinase pathways. To examine the in vivo role of BLNK, mice deficient in BLNK were generated. B cell development in BLNK-/- mice was blocked at the transition from B220+CD43+ progenitor B to B220+CD43- precursor B cells. Only a small percentage of immunoglobulin M++ (IgM++), but not mature IgMloIgDhi, B cells were detected in the periphery. Hence, BLNK is an essential component of BCR signaling pathways and is required to promote B cell development.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adaptor Proteins, Signal Transducing
-
Aging
-
Animals
-
B-Lymphocyte Subsets / cytology
-
B-Lymphocyte Subsets / immunology
-
B-Lymphocytes / cytology*
-
B-Lymphocytes / immunology
-
B-Lymphocytes / metabolism*
-
Bone Marrow Cells / cytology
-
Bone Marrow Cells / immunology
-
Carrier Proteins / genetics
-
Carrier Proteins / physiology*
-
Cell Count
-
Cell Differentiation
-
Cell Separation
-
Cell Size
-
Flow Cytometry
-
Gene Targeting
-
Hematopoietic Stem Cells / cytology*
-
Hematopoietic Stem Cells / metabolism
-
Immunoglobulin M / analysis
-
Leukopoiesis
-
Lymphoid Tissue / cytology
-
Lymphoid Tissue / immunology
-
Mice
-
Mice, Inbred C57BL
-
Phosphoproteins*
-
Receptors, Antigen, B-Cell / metabolism*
-
Second Messenger Systems
-
Signal Transduction
Substances
-
Adaptor Proteins, Signal Transducing
-
B cell linker protein
-
Carrier Proteins
-
Immunoglobulin M
-
Phosphoproteins
-
Receptors, Antigen, B-Cell