Abstract
Phototropism of Arabidopsis thaliana seedlings in response to a blue light source is initiated by nonphototropic hypocotyl 1 (NPH1), a light-activated serine-threonine protein kinase. Mutations in three loci [NPH2, root phototropism 2 (RPT2), and NPH3] disrupt early signaling occurring downstream of the NPH1 photoreceptor. The NPH3 gene, now cloned, encodes a NPH1-interacting protein. NPH3 is a member of a large protein family, apparently specific to higher plants, and may function as an adapter or scaffold protein to bring together the enzymatic components of a NPH1-activated phosphorelay.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Arabidopsis / genetics
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Arabidopsis / metabolism*
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Arabidopsis Proteins*
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Cell Membrane / metabolism
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Cloning, Molecular
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Escherichia coli
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Molecular Sequence Data
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Phosphoproteins / genetics
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Phosphoproteins / metabolism*
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Photoreceptor Cells, Invertebrate / metabolism*
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Phototropism
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Plant Proteins / genetics
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Plant Proteins / metabolism*
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Protein Binding
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Protein Serine-Threonine Kinases
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Two-Hybrid System Techniques
Substances
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Arabidopsis Proteins
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NPH3 protein, Arabidopsis
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Phosphoproteins
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Plant Proteins
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NPH1 protein, Arabidopsis
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Protein Serine-Threonine Kinases